LOCUS       pLKO.1_mito-mEmerald        7289 bp    DNA     circular UNA 29-JUL-2021
DEFINITION  .
ACCESSION   urn.local...l-do5hn2d
VERSION     urn.local...l-do5hn2d
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             535..609
                     /standard_name="COX8 presequence"
     regulatory      637..646
                     /regulatory_class="other"
                     /note="<html><body>vertebrate consensus sequence for
                     strong initiation of translation (<a
                     href=""https://www.ncbi.nlm.nih.gov/pubmed/3313277""
                     title=""https://www.ncbi.nlm.nih.gov/pubmed/3313277"">Koza
                     k, 1987</a>)</body></html>"
                     /standard_name="Kozak sequence"
     CDS             join(643..645,646..648,649..1362)
                     /transl_table=1
                     /note="<html><body>mammalian
                     codon-optimized</body></html>"
                     /product="<html><body>brightly fluorescent monomeric
                     variant of GFP</body></html>"
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGK
                     LTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
                     FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQ
                     KNGIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKR
                     DHMVLLEFVTAAGITLGMDELYK*"
                     /codon_start=1
                     /standard_name="mEmerald"
     misc_feature    1513..1746
                     /note="Geneious type: LTR"
                     /note="<html><body>self-inactivating&nbsp;3' long terminal
                     repeat (LTR) from HIV-1</body></html>"
                     /standard_name="3' LTR (ΔU3)"
     misc_feature    1818..1939
                     /note="Geneious type: polyA_signal"
                     /note="<html><body>SV40 polyadenylation
                     signal</body></html>"
                     /standard_name="SV40 poly(A) signal"
     rep_origin      1979..2114
     regulatory      complement(2135..2153)
                     /note="Geneious type: promoter"
                     /note="<html><body>promoter for bacteriophage T7 RNA
                     polymerase</body></html>"
                     /standard_name="T7 promoter"
     primer_bind     complement(2163..2179)
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
                     /standard_name="M13 fwd"
     rep_origin      2321..2776
     regulatory      2802..2906
                     /note="Geneious type: promoter"
                     /gene="<html><body><i>bla</i></body></html>"
                     /standard_name="AmpR promoter"
     CDS             join(2907..2975,2976..3767)
                     /transl_table=1
                     /note="<html><body>confers resistance to ampicillin,
                     carbenicillin, and related antibiotics</body></html>"
                     /product="<html><body>β-lactamase</body></html>"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVG
                     YIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLV
                     EYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGP
                     LLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQI
                     AEIGASLIKHW*"
                     /codon_start=1
                     /gene="<html><body><i>bla</i></body></html>"
                     /standard_name="AmpR"
     rep_origin      3938..4526
     protein_bind    4814..4835
                     /note="<html><body>CAP binding activates transcription in
                     the presence of cAMP.</body></html>"
                     /bound_moiety="<html><body><i>E. coli</i> catabolite
                     activator protein</body></html>"
                     /standard_name="CAP binding site"
     regulatory      join(4850..4855,4856..4873,4874..4880)
                     /note="Geneious type: promoter"
                     /note="<html><body>promoter for the <i>E. coli lac</i>
                     operon</body></html>"
                     /standard_name="lac promoter"
     protein_bind    4888..4904
                     /note="<html><body>The <i>lac</i> repressor binds to the
                     <i>lac</i> operator to inhibit transcription in <i>E.
                     coli</i>. This inhibition can be relieved by adding
                     lactose or isopropyl-β-D-thiogalactopyranoside
                     (IPTG).</body></html>"
                     /bound_moiety="<html><body><i>lac</i> repressor encoded by
                     <i>lacI</i></body></html>"
                     /standard_name="lac operator"
     primer_bind     4912..4928
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
                     /standard_name="M13 rev"
     regulatory      4949..4967
                     /note="Geneious type: promoter"
                     /note="<html><body>promoter for bacteriophage T3 RNA
                     polymerase</body></html>"
                     /standard_name="T3 promoter"
     regulatory      4995..5221
                     /note="Geneious type: promoter"
                     /note="<html><body>Rous sarcoma virus
                     enhancer/promoter</body></html>"
                     /standard_name="RSV promoter"
     misc_feature    5222..5402
                     /note="Geneious type: LTR"
                     /note="<html><body>truncated 5' long terminal repeat (LTR)
                     from HIV-1</body></html>"
                     /standard_name="5' LTR (truncated)"
     misc_feature    5449..5574
                     /note="<html><body>packaging signal of human
                     immunodeficiency virus type 1</body></html>"
                     /standard_name="HIV-1 Ψ"
     misc_feature    6067..6300
                     /note="<html><body>The Rev response element (RRE) of HIV-1
                     allows for Rev-dependent mRNA export from the nucleus to
                     the cytoplasm.</body></html>"
                     /standard_name="RRE"
     regulatory      6827..7067
                     /note="Geneious type: promoter"
                     /note="<html><body>RNA polymerase III promoter for human
                     U6 snRNA</body></html>"
                     /standard_name="U6 promoter"
     misc_feature    7120..7237
                     /note="<html><body>central polypurine tract and central
                     termination sequence of HIV-1</body></html>"
                     /standard_name="cPPT/CTS"
     regulatory      7286..507
                     /note="Geneious type: promoter"
                     /note="<html><body>human phosphoglycerate kinase 1
                     promoter</body></html>"
                     /standard_name="hPGK promoter"
ORIGIN      
        1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
       61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
      121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
      181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
      241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
      301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
      361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
      421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
      481 gaccgaatca ccgacctctc tccccagggg gatccgacgt caccggagct taccatgtcc
      541 gtcctgacgc cgctgctgct gcggggcttg acaggctcgg cccggcggct cccagtgccg
      601 cgcgccaaga tccattcgtt gggggatcca ccggtcgcca ccatggtgag caagggcgag
      661 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      721 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      781 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttgacc
      841 tacggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
      901 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      961 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1021 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1081 aacagccaca aggtctatat caccgccgac aagcagaaga acggcatcaa ggtgaacttc
     1141 aagacccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1201 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1261 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1321 gccgccggga tcactctcgg catggacgag ctgtacaagt aacgtacgga tatctgtaca
     1381 agtaacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat
     1441 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     1501 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     1561 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     1621 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     1681 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     1741 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     1801 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     1861 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     1921 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     1981 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     2041 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     2101 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     2161 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     2221 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     2281 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     2341 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     2401 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2461 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2521 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     2581 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     2641 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     2701 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     2761 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2821 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2881 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2941 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     3001 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     3061 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     3121 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     3181 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     3241 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     3301 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     3361 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     3421 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     3481 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     3541 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     3601 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     3661 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     3721 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     3781 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3841 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3901 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3961 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     4021 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     4081 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     4141 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     4201 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4261 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4321 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     4381 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4441 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4501 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4561 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4621 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4681 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     4741 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4801 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     4861 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     4921 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     4981 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     5041 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     5101 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     5161 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     5221 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     5281 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     5341 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     5401 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     5461 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     5521 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     5581 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     5641 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     5701 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     5761 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     5821 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     5881 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     5941 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     6001 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     6061 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     6121 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     6181 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     6241 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     6301 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     6361 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     6421 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     6481 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     6541 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     6601 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     6661 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     6721 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
     6781 acggtatcga tcacgagact agcctcgagc ggccgccccc ttcaccgagg gcctatttcc
     6841 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa
     6901 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
     6961 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac
     7021 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccggtga
     7081 attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     7141 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     7201 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     7261 agatccactt tggccgcggc tcgaggggg
//
