LOCUS       Exported                1187 bp DNA     linear   UNA 01-AUG-2021
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Sailan
  TITLE     Direct Submission
  JOURNAL   Exported Aug 1, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     RBS             21..43
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             564..581
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      600..647
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(822..850)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        963..1066
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 nnatttccct ctagaataat tttgtttaac tttaagaagg agatatacat atggcacatc
       61 cgggtcggac tggatacgac aatagggaga ttgttatgaa gtatatacac tataagttgt
      121 cacagagagg gtatgaatgg gacgccggtg acgatgtgga ggagaataga acagaagccc
      181 cagaaggcac cgagagcgag gtagtgcact tgaccctgag acaggttggg gataattttt
      241 cccgacggta ccgacgcgat ttcgctgaaa tgagcagtca acttcacctc acccctttta
      301 ctgcgcragg caggttcgca accgtggttg aagagttgtt ccgagatggc gttaactggg
      361 gacgcattgt cgccttcttc gagttcggtg gcgttatgtg tgtggaaagc gtcaaccgag
      421 aaatgagccc gcttgttgat aatatcgccc tgtggatgac agagtatctc aacagacatt
      481 tgcacacgtg gattcaggat aatggcggat gggatgcttt cgtagaattg catgggccta
      541 ctagtggagc aggatccgga tggcaccacc accaccacca ctgatgagct gagcaataac
      601 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
      661 ctatatccgg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac
      721 agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg
      781 tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat
      841 aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
      901 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      961 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     1021 gacaataacc ctgataaatg cttcataata ttgaaaaagg aagagtatga gtattcaaca
     1081 tttccgkgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     1141 agaaacgctg gtgaaagtaa agaatgctga agatcatttg ggtgcmc
//
