LOCUS       pICSL01002      2842 bp    DNA     circular UNA 3-MAR-2009
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     CDS             complement(160..1170)
                     /vntifkey="4"
                     /label="Sm/Sp no DraIII"
     Mutation        complement(193)
                     /vntifkey="62"
                     /label="G to A"
     Promoter        1442..1565
                     /label="lac promoter"
     CDS             1572..1895
                     /label=Alpha
     Promoter        complement(1857)
                     /vntifkey="30"
                     /label=P(LAC)
                     /note=" lac promoter "
     Misc._feature   complement(2228..2842)
                     /vntifkey="21"
                     /label="rep (pMB1)"
     Replication_ori 2243
                     /vntifkey="33"
                     /label=ORI
                     /note=" RNaseH cleavage point "
ORIGIN
        1 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
       61 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
      121 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TATTTGCCGA CTACCTTGGT
      181 GATCTCGCCT TTTACGTAAT GGACAAATTC TTCCAACTGA TCTGCGCGCG AGGCCAAGCG
      241 ATCTTCTTCT TGTCCAAGAT AAGCCTGTCT AGCTTCAAGT ATGACGGGCT GATACTGGGC
      301 CGGCAGGCGC TCCATTGCCC AGTCGGCAGC GACATCCTTC GGCGCGATTT TGCCGGTTAC
      361 TGCGCTGTAC CAAATGCGGG ACAACGTAAG CACTACATTT CGCTCATCGC CAGCCCAGTC
      421 GGGCGGCGAG TTCCATAGCG TTAAGGTTTC ATTTAGCGCC TCAAATAGAT CCTGTTCAGG
      481 AACCGGATCA AAGAGTTCCT CCGCCGCTGG ACCTACCAAG GCAACGCTAT GTTCTCTTGC
      541 TTTTGTCAGC AAGATAGCCA GATCAATGTC GATCGTGGCT GGCTCGAAGA TACCTGCAAG
      601 AATGTCATTG CGCTGCCATT CTCCAAATTG CAGTTCGCGC TTAGCTGGAT AACGCCACGG
      661 AATGATGTCG TCGTGCACAA CAATGGTGAC TTCTACAGCG CGGAGAATCT CGCTCTCTCC
      721 AGGGGAAGCC GAAGTTTCCA AAAGGTCGTT GATCAAAGCT CGCCGCGTTG TTTCATCAAG
      781 CCTTACGGTC ACCGTAACCA GCAAATCAAT ATCGCTGTGT GGCTTCAGGC CGCCATCCAC
      841 TGCGGAGCCG TACAAATGTA CGGCCAGCAA CGTCGGTTCG AGATGGCGCT CGATGACGCC
      901 AACTACCTCT GATAGTTGAG TCGATACTTC GGCGATCACC GCTTCCCTCA TGATGTTTAA
      961 CTTTGTTTTA GGGCGACTGC CCTGCTGCGT AACATCGTTG CTGCTCCATA ACATCAAACA
     1021 TCGACCCACG GCGTAACGCG CTTGCTGCTT GGATGCCCGA GGCATAGACT GTACCCCAAA
     1081 AAAACAGTCA TAACAAGCCA TGAAAACCGC CACTGCGCCG TTACCACCGC TGCGTTCGGT
     1141 CAAGGTTCTG GACCAGTTGC GTGAGCGCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT
     1201 TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA
     1261 AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT
     1321 TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC CACTGAAGAG
     1381 CCACTGTGGT CTCACCATTT GTCTTCGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC
     1441 GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT
     1501 ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC
     1561 AGGAAACAGC TATGACCATG ATTACGCCAA GCTTGCATGC CTGCAGGTCG ACTCTAGAGG
     1621 ATCCCCGGGT ACCGAGCTCG AATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA
     1681 ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA
     1741 ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT
     1801 GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATGGT
     1861 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA
     1921 CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG
     1981 TGACGAAGAC AAAATGTGAG ACCACGAGTG ATTAATGAAT CGGCCAACGC GCGGGGAGAG
     2041 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG
     2101 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT
     2161 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA
     2221 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA
     2281 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC
     2341 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT
     2401 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA
     2461 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG
     2521 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT
     2581 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA
     2641 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT
     2701 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC
     2761 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA
     2821 AAGGATCTCA AGAAGATCCT TT
//
