LOCUS       pICSL30015              2269 bp ds-DNA     circular     18-JAN-2017
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(160..1170)
                     /vntifkey="4"
                     /label=Sm/Sp no DraIII
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     Mutation        complement(193..193)
                     /vntifkey="62"
                     /label=G to A
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     Misc._feature   complement(1655..2269)
                     /vntifkey="21"
                     /label=rep (pMB1)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     Replication_ori 1670..1670
                     /vntifkey="33"
                     /note=" RNaseH cleavage point "
                     /label=ORI
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1395..1423
                     /label=6HIS
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
       61 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
      121 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TATTTGCCGA CTACCTTGGT
      181 GATCTCGCCT TTTACGTAAT GGACAAATTC TTCCAACTGA TCTGCGCGCG AGGCCAAGCG
      241 ATCTTCTTCT TGTCCAAGAT AAGCCTGTCT AGCTTCAAGT ATGACGGGCT GATACTGGGC
      301 CGGCAGGCGC TCCATTGCCC AGTCGGCAGC GACATCCTTC GGCGCGATTT TGCCGGTTAC
      361 TGCGCTGTAC CAAATGCGGG ACAACGTAAG CACTACATTT CGCTCATCGC CAGCCCAGTC
      421 GGGCGGCGAG TTCCATAGCG TTAAGGTTTC ATTTAGCGCC TCAAATAGAT CCTGTTCAGG
      481 AACCGGATCA AAGAGTTCCT CCGCCGCTGG ACCTACCAAG GCAACGCTAT GTTCTCTTGC
      541 TTTTGTCAGC AAGATAGCCA GATCAATGTC GATCGTGGCT GGCTCGAAGA TACCTGCAAG
      601 AATGTCATTG CGCTGCCATT CTCCAAATTG CAGTTCGCGC TTAGCTGGAT AACGCCACGG
      661 AATGATGTCG TCGTGCACAA CAATGGTGAC TTCTACAGCG CGGAGAATCT CGCTCTCTCC
      721 AGGGGAAGCC GAAGTTTCCA AAAGGTCGTT GATCAAAGCT CGCCGCGTTG TTTCATCAAG
      781 CCTTACGGTC ACCGTAACCA GCAAATCAAT ATCGCTGTGT GGCTTCAGGC CGCCATCCAC
      841 TGCGGAGCCG TACAAATGTA CGGCCAGCAA CGTCGGTTCG AGATGGCGCT CGATGACGCC
      901 AACTACCTCT GATAGTTGAG TCGATACTTC GGCGATCACC GCTTCCCTCA TGATGTTTAA
      961 CTTTGTTTTA GGGCGACTGC CCTGCTGCGT AACATCGTTG CTGCTCCATA ACATCAAACA
     1021 TCGACCCACG GCGTAACGCG CTTGCTGCTT GGATGCCCGA GGCATAGACT GTACCCCAAA
     1081 AAAACAGTCA TAACAAGCCA TGAAAACCGC CACTGCGCCG TTACCACCGC TGCGTTCGGT
     1141 CAAGGTTCTG GACCAGTTGC GTGAGCGCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT
     1201 TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA
     1261 AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT
     1321 TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC CACTGAAGAG
     1381 CCACTGTGGT CTCACCATGC ATCACCATCA CCATCACTCA ATGTGAGACC ACGAGTGATT
     1441 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT
     1501 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA
     1561 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA
     1621 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC
     1681 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA
     1741 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC
     1801 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT
     1861 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT
     1921 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG
     1981 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA
     2041 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT
     2101 ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA
     2161 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT
     2221 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTT 
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