LOCUS       pICSL30027              2491 bp ds-DNA     circular     14-FEB-2019
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(160..1170)
                     /vntifkey="4"
                     /label=Sm/Sp no DraIII
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     Mutation        complement(193..193)
                     /vntifkey="62"
                     /label=G to A
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     Misc._feature   complement(1877..2491)
                     /vntifkey="21"
                     /label=rep (pMB1)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     Replication_ori 1892..1892
                     /vntifkey="33"
                     /note=" RNaseH cleavage point "
                     /label=ORI
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1395..1645
                     /label=GB1 Solubility TAG + C3
                     /ApEinfo_fwdcolor=#feb0bf
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
       61 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
      121 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TATTTGCCGA CTACCTTGGT
      181 GATCTCGCCT TTTACGTAAT GGACAAATTC TTCCAACTGA TCTGCGCGCG AGGCCAAGCG
      241 ATCTTCTTCT TGTCCAAGAT AAGCCTGTCT AGCTTCAAGT ATGACGGGCT GATACTGGGC
      301 CGGCAGGCGC TCCATTGCCC AGTCGGCAGC GACATCCTTC GGCGCGATTT TGCCGGTTAC
      361 TGCGCTGTAC CAAATGCGGG ACAACGTAAG CACTACATTT CGCTCATCGC CAGCCCAGTC
      421 GGGCGGCGAG TTCCATAGCG TTAAGGTTTC ATTTAGCGCC TCAAATAGAT CCTGTTCAGG
      481 AACCGGATCA AAGAGTTCCT CCGCCGCTGG ACCTACCAAG GCAACGCTAT GTTCTCTTGC
      541 TTTTGTCAGC AAGATAGCCA GATCAATGTC GATCGTGGCT GGCTCGAAGA TACCTGCAAG
      601 AATGTCATTG CGCTGCCATT CTCCAAATTG CAGTTCGCGC TTAGCTGGAT AACGCCACGG
      661 AATGATGTCG TCGTGCACAA CAATGGTGAC TTCTACAGCG CGGAGAATCT CGCTCTCTCC
      721 AGGGGAAGCC GAAGTTTCCA AAAGGTCGTT GATCAAAGCT CGCCGCGTTG TTTCATCAAG
      781 CCTTACGGTC ACCGTAACCA GCAAATCAAT ATCGCTGTGT GGCTTCAGGC CGCCATCCAC
      841 TGCGGAGCCG TACAAATGTA CGGCCAGCAA CGTCGGTTCG AGATGGCGCT CGATGACGCC
      901 AACTACCTCT GATAGTTGAG TCGATACTTC GGCGATCACC GCTTCCCTCA TGATGTTTAA
      961 CTTTGTTTTA GGGCGACTGC CCTGCTGCGT AACATCGTTG CTGCTCCATA ACATCAAACA
     1021 TCGACCCACG GCGTAACGCG CTTGCTGCTT GGATGCCCGA GGCATAGACT GTACCCCAAA
     1081 AAAACAGTCA TAACAAGCCA TGAAAACCGC CACTGCGCCG TTACCACCGC TGCGTTCGGT
     1141 CAAGGTTCTG GACCAGTTGC GTGAGCGCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT
     1201 TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA
     1261 AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT
     1321 TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC CACTGAAGAG
     1381 CCACTGTGGT CTCACCATGC ATCATCATCA TCATCATAGC AGCGGCATCG AAGGCCGCGG
     1441 CCGCCAGTAC AAACTGATCC TGAACGGTAA AACCCTGAAA GGTGAAACCA CCACCGAAGC
     1501 TGTTGACGCT GCTACCGCGG AAAAAGTTTT CAAACAGTAC GCTAACGACA ACGGTGTTGA
     1561 CGGTGAATGG ACCTACGACG ACGCTACCAA AACCTTCACC GTTACCGAAA GCAGCGGCCT
     1621 GGAAGTTCTG TTTCAGGGCC CAATGTGAGA CCACGAGTGA TTAATGAATC GGCCAACGCG
     1681 CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC
     1741 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT
     1801 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA
     1861 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC
     1921 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC
     1981 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG
     2041 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA
     2101 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG
     2161 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC
     2221 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG
     2281 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT
     2341 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT
     2401 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC
     2461 GCAGAAAAAA AGGATCTCAA GAAGATCCTT T  
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