LOCUS       pETSXM2-placI-Mt        5886 bp    DNA     circular     12-APR-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|730854408|
COMMENT     VNTDBDATE|-11716773|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pETSXM2-placI-MtrC|
COMMENT     VNTAUTHORNAME|NCBI Entrez|
COMMENT     VNTAUTHORTEL|(301)496-2475|
COMMENT     VNTAUTHORFAX|(301)480-9241|
COMMENT     VNTAUTHOREML|info@ncbi.nlm.nih.gov|
COMMENT     VNTAUTHORWWW|http://www3.ncbi.nlm.nih.gov/Entrez/|
COMMENT     VNTAUTHORAD1|National Center for Biotechnology Information|
COMMENT     VNTAUTHORAD2|National Library of Medicine|
COMMENT     VNTAUTHORAD3|Building 38A, Room 8N805|
COMMENT     VNTAUTHORAD4|Bethesda, MD 20894, U.S.A.|
FEATURES             Location/Qualifiers
     misc_feature    complement(5420..5875)
                     /vntifkey="21"
                     /label=f1\origin
     misc_marker     4512..5324
                     /vntifkey="22"
                     /label=Kan
     primer          3670..3694
                     /vntifkey="27"
                     /label=pET-SpeI-F
     primer          complement(37..61)
                     /vntifkey="27"
                     /label=pET-BamHI-R
     misc_feature    68..92
                     /vntifkey="21"
                     /label=XMU-BamHI-F¡¯
     misc_feature    complement(3639..3663)
                     /vntifkey="21"
                     /label=XMU-SpeI-R
     CDS             422..1105
                     /vntifkey="4"
                     /label=ORF3-repB\from\SXM
     rep_origin      497..521
                     /vntifkey="33"
                     /label=rep-F(for\RT-PCR)
     misc_feature    670..694
                     /vntifkey="21"
                     /label=rep-R(for\RT-PCR)
     -10_signal      178..186
                     /vntifkey="47"
                     /label=Pro
     -35_signal      158..163
                     /vntifkey="48"
                     /label=pro
     RBS             384..387
                     /vntifkey="32"
                     /label=RBS
     misc_feature    3787..4406
                     /vntifkey="21"
                     /label=pBR322\ori
     promoter        complement(3285..3484)
                     /vntifkey="30"
                     /label=pLacI\(R0010)
     RBS             complement(3265..3276)
                     /vntifkey="32"
                     /label=RBS\(B0034)
     misc_feature    complement(1142..1270)
                     /vntifkey="21"
                     /label=TT
     misc_feature    complement(1285..3258)
                     /vntifkey="21"
                     /label=MtrC
BASE COUNT     1582 a      1297 c      1392 g      1615 t 
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
       61 gggatccgag cctttaggcg aactagggcg aagcccttgg tcgcggcgca gccgccagat 
      121 gctcaaattt acttgtttat ccacaggcga aacccttttt aatagatctt tcttgttttt 
      181 taagatcttt cttgttttta gcgccgctgt agcccttggt gctaaagggc tacagcggtt 
      241 taatgtggag agatttacgg taaaacagcc cctaaaggtg gagagattta cggtaaaaca 
      301 atccctaaag gtggagagat ttacggtgat attcacagtt aattgtggag aacgttgata 
      361 gcaattccac aattggttat tgtggaatcc ccaaaaataa actccacaat agtgacgtaa 
      421 aatgaacaac ctatctgtaa ctaaatcaaa tagcttgata gatgctagtt acaaactaaa 
      481 tgtacaagct caaaaactgg tcttagcttg cctcgggaaa gtagattcta gaggtaatgc 
      541 accaaaggaa atgaccctaa cagcccttga attctcagaa cttatgggga tagacatcaa 
      601 aaatgctcat agagagctat acaaggcgtc tgataccctt ttcgatgccg taatactcct 
      661 aagagatgag caagaagagg taaagcttag gtggattcaa aaaggagtga aaaaactcaa 
      721 aggacaagga gcaataacaa tcacttggac tgatgaagtt ttgaagtaca tcagtagttt 
      781 acagagcagg tttacaactt ataagctgcg acatattgcg aatctacagt cagctcattc 
      841 tattcgttta tacgaacttt tgatgaaatt taacgctact ggagaaagag ttatctatgt 
      901 ggatgacttc cgctcagcat taggtatctc cgacaaatac ccacaattta gagacttaaa 
      961 taaatgggtt atcaagccgg ccgtagatga actaaatcag cgttcagacc tcaccataaa 
     1021 ttacgagacc attaagaaag ggcgaacagt ggctgcattg tcatttgagt tcaagcagag 
     1081 cgcacagcta aaattagacg tataatcagt cgacccatgg ctgcagcggc cgctactagt 
     1141 atataaacgc agaaaggccc acccgaaggt gagccagtgt gactctagta gagagcgttc 
     1201 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt 
     1261 tgatgcctgg ctctagtaca tatgttacat tttcacttga gtgtgatctg caactgttgg 
     1321 ggtatggcag tagaaacagg tttctgactg tgccgcttgg ttggcttgag tataatcacc 
     1381 atcaataaca gcaccgtagc ttacaagggt gtcgacagaa tgtttggtca ttttatctgt 
     1441 accaatggta tgacatgagg tacaagtggc tgtgattggg gtggtatagc cttttgttgt 
     1501 cgctagtgca cctttcagtt taaatgcatc caaattgaag tcgttatgac attgggtaca 
     1561 ttctttaatg ataccttgct caccatgcac cacgtgtagt ttcatctcta atgcaccgag 
     1621 tttagtcggg ctataggtac cgtctggtgt atggcatgcc acacaagcat caacgccgac 
     1681 gataggctta ccgcttgcat ctttggcatg tgaaacttgt tcagacatta caaagcctgc 
     1741 atggtggcct ttgtggattt caaaggtgtc gccgtgacag ttttggcatg tcgcaaactc 
     1801 aaccgagtta acatggcgca tgcttggtgc tttgccagac ttagtgccaa aggctaactc 
     1861 ggctttcata ctggtggttg tgctattagc ggtacaagct gttagatctg tgccggtatt 
     1921 gcacatttct agaccgatga agctaaaggc ggtatcagta tcgcccgtgc caaatggcag 
     1981 ggcaggtgtg gtgaacacca gtttaccgtc aactacgctg acatctgctt gcagtgcgcc 
     2041 atccttgact aagtctttag tcactttctt atattgtgtt actgggcttg ggttatagcc 
     2101 catgatgggg aagttaggac caacgttagt gatagtttct aaacgtttga tcttattttg 
     2161 tatgctcgcc gcatcaatgg cattgccgtc tttatctaag atagacacgg ttaataccgc 
     2221 agtattatca gcttgagcag ctaacgttgc agacatacct aattgagcga taacggcttt 
     2281 tttgtcagcg gttttagctg tatgtaactc ggctgtccaa tcactcttat gacaggcgat 
     2341 acagtttgag ttatcgagct gttgtgagtg gcctttaccc gcagcaaaat cgatgtctac 
     2401 gtgacagcta gaacagactt ccattgttgg aactcgtgac cagttttttg cctcggtaag 
     2461 catgtcggat tcaatgtgac acacttggca attatcttgg acaatttttt gcgcaacttc 
     2521 agggacttta ttgtttttgc cccaagcttt gttggcatta tgcacattgt gaattaagtg 
     2581 actaaaggcg acatggggtt taccgcgacc atcagcaaac tcttgtgtgt ggcaagatat 
     2641 acaggtttcg acttcagttg cttggtgata aatcttttca ccttcaacgt ggcaagaagc 
     2701 acatacttcg tggctaacga tatttttggt gtattgcgga gcattgcctt ggccgtcgaa 
     2761 atcttcaacc atttcggcaa cggggacggt cgttccgtct gctaacttac ccgcagcaga 
     2821 aacaacgtta aaccgttgcg ttaattgggc attaaaaact ttattgctat cgaaggcgtc 
     2881 gaatttaaag gtatagctac cgtttttatt atcgacatag ctctttgaag agcctaatcc 
     2941 ttgccagtta gcactattac cagggcctgt cgccccttcc ggtattaatt gcagtgcttt 
     3001 tttgatttct aaatttgcga gacccacaat tggcatatcg gcttcgttgg tagcgaaaac 
     3061 agtgaccaca ggcgaaccgt tttcatagct cactttcgtg atatcgaggt ttaccttatg 
     3121 gatagtacca gcgggttctc cacctggctc accagggtta ccatcggtac catcgctacc 
     3181 accacaacca gttaaggcca tggagatggc acttgctgcg agcagcagtg cgatttttga 
     3241 tttttttgcg ttcatcatga gctctttctc ctctttctct agtatgtgtg aaattgttat 
     3301 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 
     3361 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 
     3421 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 
     3481 attgctctag aagcggccgc gaattccaga aatcatcctt agcgaaagct aaggattttt 
     3541 tttatctgaa attctgcctc gtgatacgcc tatttttata ggttaatgtc atgataataa 
     3601 tggtttctta gacgtcaggt ggcactcgag aatgcaacac acgcaacacc gcatctcttg 
     3661 atgactagtg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 
     3721 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 
     3781 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 
     3841 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 
     3901 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 
     3961 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 
     4021 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 
     4081 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 
     4141 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 
     4201 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 
     4261 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 
     4321 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 
     4381 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 
     4441 ctcacgttaa gggattttgg tcatgaacaa taaaactgtc tgcttacata aacagtaata 
     4501 caaggggtgt tatgagccat attcaacggg aaacgtcttg ctctaggccg cgattaaatt 
     4561 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag 
     4621 gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg 
     4681 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg 
     4741 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac 
     4801 tcaccactgc gatccccggg aaaacagcat tccaggtatt agaagaatat cctgattcag 
     4861 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt 
     4921 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga 
     4981 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac 
     5041 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg 
     5101 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg 
     5161 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg 
     5221 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg 
     5281 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaagaa ttaattcatg 
     5341 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 
     5401 ccccgaaaag tgccacctga aattgtaaac gttaatattt tgttaaaatt cgcgttaaat 
     5461 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa 
     5521 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta 
     5581 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 
     5641 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 
     5701 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 
     5761 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 
     5821 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat 
     5881 tcgcca 
//
