LOCUS       PB-mCar-EGFP-WPRE-BGH-p 8566 bp ds-DNA     circular     03-AUG-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(263..569)
                     /label="F1 ori"
     CDS             complement(588..656)
                     /label="LacZ alpha"
     primer_bind     727..744
                     /label="M13-fwd"
     misc_feature    870..1111
                     /label="3' TF long_misc_feature"
     misc_feature    874..936
                     /label="3' TF short_misc_feature"
     misc_feature    1113..1344
                     /label="Core insulator_misc_feature"
     primer_bind     1278..1297
                     /label="primer_bind"
     misc_feature    1364..1884
                     /label="mCar-Promoter"
     misc_feature    1885..1890
                     /label="unique XbaI site"
     misc_feature    1897..2616
                     /label="eGFP"
     misc_feature    2624..3212
                     /label="WPRE"
     misc_feature    3213..3218
                     /label="unique ECO site"
     misc_feature    3219..3443
                     /label="BGH pA"
     misc_feature    3444..3450
                     /label="unique CLAI site"
     misc_feature    3451..4668
                     /label="UBC_Promoter"
     misc_feature    3831..4061
                     /label="Core insulator_misc_feature"
     misc_feature    4062..4374
                     /label="5' TR long_misc_feature"
     misc_feature    4336..4370
                     /label="5' TR_misc_feature"
     misc_feature    4700..5098
                     /label="BlastiR"
     misc_feature    5137..5267
                     /label="SV 40 POLY(A) [3']"
     primer_bind     complement(6541..6561)
                     /label="M13-rev"
     misc_binding    complement(6567..6589)
                     /label="LacO"
     promoter        complement(6593..6623)
                     /label="lac promoter"
     rep_origin      complement(6929..7611)
                     /label="ColE1 origin"
     CDS             complement(7709..8368)
                     /label="AmpR"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1081 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
     1141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     1201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     1261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     1321 acacctgggg ggatacgggg aaaaggcctc cacggccact agtGGTTCTT CCCATTTTGG
     1381 CTACATGGTC TTTTTTTTTA CCTTTTTGGT TCCTTTGGCC TTTTGGCTTT TGGCTTCCAG
     1441 GGCTTCTGGA TCCCCCCCAA CCCCTCCCAT ACACATACAC ATGTGCACTC GTGCACTCAA
     1501 CCCAGCACAG GATAATGTTC ATTCTTGACC TTTCCACATA CATCTGGCTA TGTTCTCTCT
     1561 CTTATCTACA ATAAATCTCC TCCACTATAC TTAGGAGCAG TTATGTTCTT CTTCTTTCTT
     1621 TCTTTTTTTT TTTTTTCATT CAGTAACATC ATCAGAATCC CCTAGCTCTG GCCTACCTCC
     1681 TCAGTAACAA TCAGCTGATC CCTGGCCACT AATCTGTACT CACTAATCTG TTTTCCAAAC
     1741 TCTTGGCCCC TGAGCTAATT ATAGCAGTGC TTCATGCCAC CCACCCCAAC CCTATTCTTG
     1801 TTCTCTGACT CCCACTAATC TACACATTCA GAGGATTGTG GATATAAGAG GCTGGGAGGC
     1861 CAGCTTAGCA ACCAGAGCTG GAGGtctaga gccaccATGG TGAGCAAGGG CGAGGAGCTG
     1921 TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC
     1981 AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC
     2041 TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC
     2101 GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC
     2161 ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG
     2221 ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC
     2281 ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC
     2341 CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC
     2401 CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC
     2461 ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG
     2521 AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC
     2581 GGGATCACTC TCGGCATGGA CGAGCTGTAc aAGTGAggat cctaatcaac ctctggatta
     2641 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     2701 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     2761 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     2821 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     2881 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     2941 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     3001 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     3061 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     3121 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     3181 gagtcggatc tccctttggg ccgcctcccc gcgaattcCT GTGCCTTCTA GTTGCCAGCC
     3241 ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT
     3301 CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT
     3361 GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC
     3421 TGGGGATGCG GTGGGCTCTA TGGatcgatg atctggcctc cgcgccgggt tttggcgcct
     3481 cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcaggagc
     3541 gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct
     3601 tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac
     3661 tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg
     3721 cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg
     3781 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg
     3841 atcgtcactt ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc
     3901 gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc
     3961 aaggttgccc tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc
     4021 ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg
     4081 gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg
     4141 gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga
     4201 actcggtttg tcgtctgttg cgggggcggc agttatgcgg tgccgttggg cagtgcaccc
     4261 gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata
     4321 atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc
     4381 agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc tggtgagggg
     4441 agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt cttaagtagc
     4501 tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt
     4561 aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt tagactagta
     4621 aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacgaagCT TGGGCTGCAG
     4681 GTCGACTCTA GATGCCACCA tggccaagcc tttgtctcaa gaagaatcca ccctcattga
     4741 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc
     4801 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
     4861 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
     4921 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
     4981 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
     5041 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaagt
     5101 cgacaatcaa cctctggatt acaaaatttg tgggttaact tgtttattgc agcttataat
     5161 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5221 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggaa ttgactcaaa
     5281 tgatgtcaat tagtctatca gaagctatct ggtctccctt ccgggggaca agacatccct
     5341 gtttaatatt taaacagcag tgttcccaaa ctgggttctt atatcccttg ctctggtcaa
     5401 ccaggttgca gggtttcctg tcctcacagg aacgaagtcc ctaaagaaac agtggcagcc
     5461 aggtttagcc ccggaattga ctggattcct tttttagggc ccattggtat ggctttttcc
     5521 ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg
     5581 atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg
     5641 gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga
     5701 gggacgtaat tacatccctg ggggctttgg gggggggctg tccctgatat ctataacaag
     5761 aaaatatata tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg
     5821 tatgagttaa atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg
     5881 tcgttatagt tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc
     5941 caagcggcga ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga
     6001 gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaatc
     6061 tagctgcatc aggatcatat cgtcgggtct tttttccggc tcagtcatcg cccaagctgg
     6121 cgctatctgg gcatcgggga ggaagaagcc cgtgcctttt cccgcgaggt tgaagcggca
     6181 tggaaagagt ttgccgagga tgactgctgc tgcattgacg ttgagcgaaa acgcacgttt
     6241 accatgatga ttcgggaagg tgtggccatg cacgccttta acggtgaact gttcgttcag
     6301 gccacctggg ataccagttc gtcgcggctt ttccggacac agttccggat ggtcagcccg
     6361 aagcgcatca gcaacccgaa caataccggc gacagccgga actgccgtgc cggtgtgcag
     6421 attaatgaca gcggtgcggc gctgggatat tacgtcagcg aggacgggta tcctggctgg
     6481 atgccgcaga aatggacatg gataccccgt gagttacccg gcgggcgcgc ttggcgtaat
     6541 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6601 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6661 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6721 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6781 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6841 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6901 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6961 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7021 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7081 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7141 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7201 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7261 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7321 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7381 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7441 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7501 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7561 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7621 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7681 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7741 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7801 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7861 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7921 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7981 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     8041 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8101 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8161 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8221 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8281 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     8341 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8401 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8461 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8521 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcat
//