LOCUS       pB80\(B-actin)          6155 bp    DNA     circular     17-FEB-2015
SOURCE      
  ORGANISM  
COMMENT     Custom cloning vector from the Hoogenraad lab. Drives expression from a B-actin promotor.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|722434724|
COMMENT     VNTDBDATE|725875687|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pB80 (B-actin)|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT         "MluI" "NarI" "NcoI" "NdeI" "NheI" "NotI" "PacI" "PmeI" "PstI" "SacI" 
COMMENT         "SacII" "SnaBI" "SpeI" "SphI" "XbaI" "XhoI" "XmaI" "AscI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT        #2=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 6 2116 0 3362 0 3859 0 
COMMENT            5167 0 5179 0 5563 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AvrII" "cctagg" 2 0 1 1134 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 4 761 0 804 0 1107 0 3921 0 
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COMMENT        #6=(CRSite (CStringList) "BglII" "agatct" 2 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "BspEI" "tccgga" 2 0 2 1151 0 4241 0 0 "") 
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COMMENT        #9=(CRSite (CStringList) "EagI" "cggccg" 2 0 2 345 0 1118 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 1030 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1006 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "HpaI" "gttaac" 4 0 2 1178 0 1311 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "KasI" "ggcgcc" 2 0 4 757 0 800 0 1103 0 3917 
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COMMENT        #16=(CRSite (CStringList) "MfeI" "caattg" 2 0 1 1298 0 0 "") 
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COMMENT        #18=(CRSite (CStringList) "NarI" "ggcgcc" 3 0 4 758 0 801 0 1104 0 3918 
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COMMENT        #21=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 997 0 0 "") 
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COMMENT        #23=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 1 4528 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "PstI" "ctgcag" 6 0 6 1115 0 4663 0 4711 0 
COMMENT             4979 0 5266 0 5658 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 1065 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 3 365 0 1079 0 4393 0 0 
COMMENT             "") #28=(CRSite (CStringList) "SnaBI" "tacgta" 4 0 1 1162 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 1048 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "SphI" "gcatgc" 6 0 10 4898 0 5371 0 5375 0 
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COMMENT        #32=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 1058 0 5875 0 0 "") 
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COMMENT                          "Foreign object. Author: Demo User. Original author: Demo User" 
COMMENT                          1) 
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FEATURES             Location/Qualifiers
     CDS             2621..3481
                     /vntifkey="4"
                     /label=Amp\(r)
     promoter        4022..6152
                     /vntifkey="29"
                     /label=B-actin\promoter
     primer_bind     933..955
                     /vntifkey="28"
                     /label=pB80\fwd
     primer_bind     1256..1279
                     /vntifkey="28"
                     /label=pB80\rev
BASE COUNT     1240 a      1774 c      1781 g      1360 t 
ORIGIN
        1 cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc cgccccggct 
       61 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg 
      121 taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg aaagccttaa 
      181 agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg tgcgtgtgtg 
      241 tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc gctgcgggcg 
      301 cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg gggcggtgcc 
      361 ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg 
      421 ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca cccccctccc 
      481 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg tggcgcgggg 
      541 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct 
      601 cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc ggctgtcgag 
      661 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc 
      721 ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg 
      781 gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg 
      841 tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag ggggacggct 
      901 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggggttt 
      961 atatcttccc ttctctgttc ctccgcagcc cccaagctag cgctaagctt gccaccatgg 
     1021 gcgcgcctga attcaggatc cagtcgacta gtacttctcg agctctgtac atgtccgcgg 
     1081 tcgcgacgta cgcgtatcga tggcgccagc tgcaggcggc cgccatatgc atcctaggcc 
     1141 tattaatatt ccggagtata cgtagccggc taacgttaac aaccggtacc tctagataag 
     1201 taatgatcat aatcagccat atcacatctg tagaggtttt acttgcttta aaaaacctcc 
     1261 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta 
     1321 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 
     1381 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 
     1441 ggatcaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 
     1501 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 
     1561 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 
     1621 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 
     1681 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 
     1741 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 
     1801 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 
     1861 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 
     1921 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 
     1981 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 
     2041 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 
     2101 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 
     2161 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 
     2221 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 
     2281 ctaactacgg ctacagtaga aggacagtat ttggtatctg cgctctgctg aagccagtta 
     2341 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 
     2401 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 
     2461 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 
     2521 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 
     2581 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 
     2641 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 
     2701 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 
     2761 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 
     2821 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 
     2881 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 
     2941 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 
     3001 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 
     3061 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 
     3121 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 
     3181 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 
     3241 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 
     3301 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 
     3361 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 
     3421 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 
     3481 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 
     3541 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 
     3601 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 
     3661 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 
     3721 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 
     3781 gtcagggcgc gtcagcgggt gttggcgggt gtcggggttg gcttaactat gcggcatcag 
     3841 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 
     3901 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 
     3961 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 
     4021 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 
     4081 agttgggatc tttgcattgg cccacggctc tcaggatggg gatgctcccc ttcagcaccc 
     4141 ggttcccctt ggaaactgat ggtcctggct ctgtggcatg gcagtggcac tgtgaggagc 
     4201 ccctaccagc agcacacagt gggtttggca ctgccacgct ccggatgccg cgctctgatc 
     4261 caaccccata atcaagggaa cccgaattgc cccatcattg cccccaccac ccccatcctg 
     4321 ccgggccctc acaccccacg ctgccttgtg gtgacattcc ccagcccaaa cccacggctt 
     4381 catggctacc gcggggcatt tcccattgcc gccccattat cagctctgca cacctcccgc 
     4441 tgtacccatg cctcgtggct gcccttcttt gacgtataat cttctaatta atacccggcc 
     4501 ttgtcaaagt ggagcacaaa cgttaattaa ttccccagca ggcaggtaat taacagtgtg 
     4561 actccctttt tgctgcgagt ggggctgata cagagagatg tggcactatg gagcccacgg 
     4621 ggtcctggca ctgggtgccc acggaggtcc ccatgtgctg cagtgtcacc gcctccgagg 
     4681 tgacagtatt gtccctgcgg tgtccctgca gctcagctct gtccacaggg ccacctccag 
     4741 tttggagggg acacaatgca gccccgatgc aacccatcct cgcagcatcc cagggacaaa 
     4801 gaccccactg caagaccgca cacagggctg ggtcccgctc ccctaatatc tacagtgctt 
     4861 ttgcatggcc ccttaatcaa tgcagttaat cagcatgcgc tcatgcaccg ctctggagct 
     4921 gcaaagcccc tcgcagcgct gctcaccaac accgcgcacc gccccggccc agcctgcagc 
     4981 acgcgctgca aacaggaaag aaacaaaata ttgcccaaat gtaggcaaag gcattcggct 
     5041 gccttgacct ccgccgggcc gggccctgcc tgactcagct ccttactcag cgctcgcttc 
     5101 ctccctccgg ctgccaccgc cgcagcgcac accctgacaa agagtggccc ttaacgggct 
     5161 ctgaggtgca cccagcagtg cactcagcag tccaagggcc ggcctggagg tttgcaccgc 
     5221 tacgtgctga cattagcatt gaacttggcc ctgggtagtg ctgcaggccg ggcggggtgg 
     5281 gtgtagagag tgcagcgcgc gttgcacccg gtgccccttc ccctcccttg catcccagca 
     5341 ggctgcaccc cagcaccagg cccgtgcatg catgctcctg gtgttattgc agcctggtgc 
     5401 atgcatgcgt cttagtggtg cagcgctgtg catgcatcct ccttggtgtg tagcagctta 
     5461 gtgcatgcat acccctcggt gttattgctg ctctgtgcag gcacgctcat tgtatcactt 
     5521 catcccagtg catgcactca cactggagcg attgctgctc ggtgcacgca cactcattgt 
     5581 atcacgtcag ctcagtggct gcacgcacac cggtgttatt gctgctcggt gcgtgcatgc 
     5641 acatcagtgt cgctgcagct cagtgcatgc acgctcattg cccatcgcta tccctgcctc 
     5701 tcctgctggc gctccccggg aggtgacttc aaggggaccg caggaccacc tcgggggtgg 
     5761 ggggagggct gcacacgcgg accccgctcc ccctccccaa caaagcactg tggaatcaaa 
     5821 aaggggggag gggggatgga ggggcgcgtc acacccccgc cccacaccct cacctcgagg 
     5881 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt 
     5941 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggcgcgcg 
     6001 ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca 
     6061 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg 
     6121 ccctataaaa agcgaagcgc gcggcgggcg ggagt 
//
