LOCUS       pB80-FKBP-mCherr        7864 bp    DNA     circular     18-AUG-2021
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14004780|
COMMENT     VNTDBDATE|-14004780|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pB80-FKBP-mCherry-RAB11|
COMMENT     VNTAUTHORNAME|Wilco Nijenhuis|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #29=(CRSite (CStringList) "SacI" "gagctc" 6 0 0 0 "") 
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FEATURES             Location/Qualifiers
     promoter        5734..7864
                     /vntifkey="29"
                     /label=B-actin\promoter
     CDS             4333..5193
                     /vntifkey="4"
                     /label=Amp\(r)
     primer_bind     2968..2991
                     /vntifkey="28"
                     /label=pB80\rev
     misc_feature    1019..1021
                     /vntifkey="21"
                     /label=Kozak
     CDS             1022..1345
                     /vntifkey="4"
                     /label=FKBP
     primer_bind     936..958
                     /vntifkey="28"
                     /label=pB80\fwd
     CDS             2249..2896
                     /vntifkey="4"
                     /label=RAB11
     CDS             1445..2149
                     /vntifkey="4"
                     /label=mCherry
BASE COUNT     1679 a      2187 c      2317 g      1681 t 
ORIGIN
        1 agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc gcccgccccg 
       61 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg 
      121 ctgtaattag cgcttggttt aatgacggct cgtttctttt ctgtggctgc gtgaaagcct 
      181 taaagggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt 
      241 gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg agcgctgcgg 
      301 gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc cgggggcggt 
      361 gccccgcggt gcgggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg 
      421 gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta acccccccct gcacccccct 
      481 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg gcgtggcgcg 
      541 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg 
      601 cctcgggccg gggagggctc gggggagggg cgcggcggcc ccggagcgcc ggcggctgtc 
      661 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac 
      721 ttcctttgtc ccaaatctgg cggagccgaa atctgggagg cgccgccgca ccccctctag 
      781 cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt 
      841 gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgccg cagggggacg 
      901 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcgggg 
      961 tttatatctt cccttctctg ttcctccgca gcccccaagc taggcaattg ggcgcgccac 
     1021 catgggagtg caggtggaga ctatctcccc aggagacggg cgcaccttcc ccaagcgcgg 
     1081 ccagacctgc gtggtgcact acaccgggat gcttgaagat ggaaagaaat ttgattcctc 
     1141 ccgggacaga aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg 
     1201 ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc aaactgacta tatctccaga 
     1261 ttatgcctat ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt 
     1321 cgatgtggag cttctaaaac tggaatctag aggtggcggg ggcagtgggg gagggggaag 
     1381 tggaggcggc ggttctggcg ggggaggctc cggtggcggg gggagcgggg gtgggagtaa 
     1441 gcttgtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa 
     1501 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg 
     1561 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc 
     1621 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca 
     1681 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg 
     1741 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga 
     1801 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggtcccgt 
     1861 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg 
     1921 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc 
     1981 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt 
     2041 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga 
     2101 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg ctagcggagg 
     2161 cggtggctca ggcggaggag ggtcaggagg gggaggcagt ggcgggggcg gctctggagg 
     2221 agggggttct ggtggtggta gctacgtagg cacccgcgac gacgagtacg actatctctt 
     2281 caaagttgtc cttattggag attctggtgt tggaaagagt aatctcttgt ctcgatttac 
     2341 tcgaaatgag tttaatctcg aaagtaagag caccattgga gtagagtttg caacaagaag 
     2401 catccaggtt gatgggaaaa caataaaggc acagatatgg gacacggcag ggcaggagcg 
     2461 ataccgagct ataacatcag catattatcg tggagctgta ggtgccttac tggtttatga 
     2521 cattgctaag catctcacat atgaaaatgt agaacgatgg ctgaaagaac tgagagatca 
     2581 tgctgatagt aacattgtta tcatgcttgt gggcaataag agtgatttgc gtcatctcag 
     2641 ggcagttcct acagatgaag caagagcttt tgcagaaaag aatggtttgt cattcattga 
     2701 gacttccgct ctagactcta caaatgtaga agctgctttt cagacaattc tgacagagat 
     2761 ataccgcatt gtttcccaga aacaaatgtc agacagacgt gaaaatgaca tgtctccaag 
     2821 caacaatgtg gttcctattc atgttccacc aaccactgaa aacaagccaa aggtgcagtg 
     2881 ctgtcagaac atctaaaccg gtcctagata agtaatgatc ataatcagcc atatcacatc 
     2941 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa 
     3001 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag 
     3061 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 
     3121 gtccaaactc atcaatgtat cttatcatgt ctggatcaat tcgtaatcat ggtcatagct 
     3181 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 
     3241 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 
     3301 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 
     3361 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 
     3421 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 
     3481 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 
     3541 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 
     3601 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 
     3661 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 
     3721 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 
     3781 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 
     3841 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 
     3901 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 
     3961 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacagta gaaggacagt 
     4021 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 
     4081 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 
     4141 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 
     4201 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 
     4261 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 
     4321 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 
     4381 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 
     4441 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 
     4501 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 
     4561 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 
     4621 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 
     4681 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 
     4741 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 
     4801 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 
     4861 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 
     4921 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 
     4981 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 
     5041 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 
     5101 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 
     5161 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 
     5221 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 
     5281 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 
     5341 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg 
     5401 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 
     5461 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 
     5521 gtgtcggggt tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 
     5581 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc 
     5641 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 
     5701 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca 
     5761 gtcacgacgt tgtaaaacga cggccagtgc caagttggga tctttgcatt ggcccacggc 
     5821 tctcaggatg gggatgctcc ccttcagcac ccggttcccc ttggaaactg atggtcctgg 
     5881 ctctgtggca tggcagtggc actgtgagga gcccctacca gcagcacaca gtgggtttgg 
     5941 cactgccacg ctccggatgc cgcgctctga tccaacccca taatcaaggg aacccgaatt 
     6001 gccccatcat tgcccccacc acccccatcc tgccgggccc tcacacccca cgctgccttg 
     6061 tggtgacatt ccccagccca aacccacggc ttcatggcta ccgcggggca tttcccattg 
     6121 ccgccccatt atcagctctg cacacctccc gctgtaccca tgcctcgtgg ctgcccttct 
     6181 ttgacgtata atcttctaat taatacccgg ccttgtcaaa gtggagcaca aacgttaatt 
     6241 aattccccag caggcaggta attaacagtg tgactccctt tttgctgcga gtggggctga 
     6301 tacagagaga tgtggcacta tggagcccac ggggtcctgg cactgggtgc ccacggaggt 
     6361 ccccatgtgc tgcagtgtca ccgcctccga ggtgacagta ttgtccctgc ggtgtccctg 
     6421 cagctcagct ctgtccacag ggccacctcc agtttggagg ggacacaatg cagccccgat 
     6481 gcaacccatc ctcgcagcat cccagggaca aagaccccac tgcaagaccg cacacagggc 
     6541 tgggtcccgc tcccctaata tctacagtgc ttttgcatgg ccccttaatc aatgcagtta 
     6601 atcagcatgc gctcatgcac cgctctggag ctgcaaagcc cctcgcagcg ctgctcacca 
     6661 acaccgcgca ccgccccggc ccagcctgca gcacgcgctg caaacaggaa agaaacaaaa 
     6721 tattgcccaa atgtaggcaa aggcattcgg ctgccttgac ctccgccggg ccgggccctg 
     6781 cctgactcag ctccttactc agcgctcgct tcctccctcc ggctgccacc gccgcagcgc 
     6841 acaccctgac aaagagtggc ccttaacggg ctctgaggtg cacccagcag tgcactcagc 
     6901 agtccaaggg ccggcctgga ggtttgcacc gctacgtgct gacattagca ttgaacttgg 
     6961 ccctgggtag tgctgcaggc cgggcggggt gggtgtagag agtgcagcgc gcgttgcacc 
     7021 cggtgcccct tcccctccct tgcatcccag caggctgcac cccagcacca ggcccgtgca 
     7081 tgcatgctcc tggtgttatt gcagcctggt gcatgcatgc gtcttagtgg tgcagcgctg 
     7141 tgcatgcatc ctccttggtg tgtagcagct tagtgcatgc atacccctcg gtgttattgc 
     7201 tgctctgtgc aggcacgctc attgtatcac ttcatcccag tgcatgcact cacactggag 
     7261 cgattgctgc tcggtgcacg cacactcatt gtatcacgtc agctcagtgg ctgcacgcac 
     7321 accggtgtta ttgctgctcg gtgcgtgcat gcacatcagt gtcgctgcag ctcagtgcat 
     7381 gcacgctcat tgcccatcgc tatccctgcc tctcctgctg gcgctccccg ggaggtgact 
     7441 tcaaggggac cgcaggacca cctcgggggt ggggggaggg ctgcacacgc ggaccccgct 
     7501 ccccctcccc aacaaagcac tgtggaatca aaaagggggg aggggggatg gaggggcgcg 
     7561 tcacaccccc gccccacacc ctcacctcga ggtgagcccc acgttctgct tcactctccc 
     7621 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc 
     7681 agcgatgggg gcgggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc 
     7741 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt 
     7801 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg 
     7861 cggg 
//
