LOCUS       pB80-LAMP1-mCher        8584 bp    DNA     circular     18-AUG-2021
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14004794|
COMMENT     VNTDBDATE|-14004794|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pB80-LAMP1-mCherry-iLID.gb|
COMMENT     VNTAUTHORNAME|Wilco Nijenhuis|
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FEATURES             Location/Qualifiers
     promoter        6454..8584
                     /vntifkey="29"
                     /label=B-actin\promoter
     primer_bind     936..958
                     /vntifkey="28"
                     /label=pB80\fwd
     CDS             5053..5913
                     /vntifkey="4"
                     /label=Amp\(r)
     primer_bind     3688..3711
                     /vntifkey="28"
                     /label=pB80\rev
     CDS             3176..3613
                     /vntifkey="4"
                     /label=iLID
     CDS             1022..2272
                     /vntifkey="4"
                     /label=LAMP1
     CDS             2372..3076
                     /vntifkey="4"
                     /label=mCherry
BASE COUNT     1808 a      2466 c      2511 g      1799 t 
ORIGIN
        1 agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc gcccgccccg 
       61 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg 
      121 ctgtaattag cgcttggttt aatgacggct cgtttctttt ctgtggctgc gtgaaagcct 
      181 taaagggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt 
      241 gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg agcgctgcgg 
      301 gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc cgggggcggt 
      361 gccccgcggt gcgggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg 
      421 gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta acccccccct gcacccccct 
      481 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg gcgtggcgcg 
      541 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg 
      601 cctcgggccg gggagggctc gggggagggg cgcggcggcc ccggagcgcc ggcggctgtc 
      661 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac 
      721 ttcctttgtc ccaaatctgg cggagccgaa atctgggagg cgccgccgca ccccctctag 
      781 cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt 
      841 gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgccg cagggggacg 
      901 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcgggg 
      961 tttatatctt cccttctctg ttcctccgca gcccccaagc taggcaattg ggcgcgccac 
     1021 catggcggcc cccggcagcg cccggcgacc cctgctgctg ctactgctgt tgctgctgct 
     1081 cggcctcatg cattgtgcgt cagcagcaat gtttatggtg aaaaatggca acgggaccgc 
     1141 gtgcataatg gccaacttct ctgctgcctt ctcagtgaac tacgacacca agagtggccc 
     1201 taagaacatg acctttgacc tgccatcaga tgccacagtg gtgctcaacc gcagctcctg 
     1261 tggaaaagag aacacttctg accccagtct cgtgattgct tttggaagag gacatacact 
     1321 cactctcaat ttcacgagaa atgcaacacg ttacagcgtc cagctcatga gttttgttta 
     1381 taacttgtca gacacacacc ttttccccaa tgcgagctcc aaagaaatca agactgtgga 
     1441 atctataact gacatcaggg cagatataga taaaaaatac agatgtgtta gtggcaccca 
     1501 ggtccacatg aacaacgtga ccgtaacgct ccatgatgcc accatccagg cgtacctttc 
     1561 caacagcagc ttcagcaggg gagagacacg ctgtgaacaa gacaggcctt ccccaaccac 
     1621 agcgccccct gcgccaccca gcccctcgcc ctcacccgtg cccaagagcc cctctgtgga 
     1681 caagtacaac gtgagcggca ccaacgggac ctgcctgctg gccagcatgg ggctgcagct 
     1741 gaacctcacc tatgagagga aggacaacac gacggtgaca aggcttctca acatcaaccc 
     1801 caacaagacc tcggccagcg ggagctgcgg cgcccacctg gtgactctgg agctgcacag 
     1861 cgagggcacc accgtcctgc tcttccagtt cgggatgaat gcaagttcta gccggttttt 
     1921 cctacaagga atccagttga atacaattct tcctgacgcc agagaccccg cctttaaagc 
     1981 tgccaacggc tccctgcgag cgctgcaggc cacagtcggc aattcctaca agtgcaacgc 
     2041 ggaggagcac gtccgtgtca cgaaggcgtt ttcagtcaat atattcaaag tgtgggtcca 
     2101 ggctttcaag gtggaaggtg gccagtttgg ctctgtggag gagtgtctgc tggacgagaa 
     2161 cagcatgctg atccccatcg ctgtgggtgg tgccctggcg gggctggtcc tcatcgtcct 
     2221 catcgcctac ctcgtcggca ggaagaggag tcacgcaggc taccagacta tctctagagg 
     2281 tggcgggggc agtgggggag ggggaagtgg aggcggcggt tctggcgggg gaggctccgg 
     2341 tggcgggggg agcgggggtg ggagtaagct tgtgagcaag ggcgaggagg ataacatggc 
     2401 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga 
     2461 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct 
     2521 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat 
     2581 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc 
     2641 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac 
     2701 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg 
     2761 caccaacttc ccctccgacg gtcccgtaat gcagaagaag accatgggct gggaggcctc 
     2821 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa 
     2881 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc 
     2941 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga 
     3001 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat 
     3061 ggacgagctg tacaaggcta gcggaggcgg tggctcaggc ggaggagggt caggaggggg 
     3121 aggcagtggc gggggcggct ctggaggagg gggttctggt ggtggtagct acgtactggc 
     3181 aaccacactg gaacggatcg agaaaaattt cgtgattact gatccgagac tgcctgacaa 
     3241 cccaatcatt tttgcgagcg attccttcct gcagctgaca gaatattctc gggaagagat 
     3301 cctggggcgc aattgccgtt ttctgcaggg acccgagaca gaccgtgcca ctgttcggaa 
     3361 aatcagagat gctattgaca accagactga agtgaccgtt cagctgatca attataccaa 
     3421 gagcggcaag aagttctgga acgtgttcca cctgcagccg atgcgcgatt ataagggcga 
     3481 cgtccagtac ttcattggcg tgcagctgga tggcaccgaa cgtcttcatg gcgccgctga 
     3541 gcgtgaggcg gtctgcctga tcaaaaagac agcctttcag attgctgagg cagcgaacga 
     3601 cgaaaattac ttttgaaccg gtcctagata agtaatgatc ataatcagcc atatcacatc 
     3661 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa 
     3721 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag 
     3781 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 
     3841 gtccaaactc atcaatgtat cttatcatgt ctggatcaat tcgtaatcat ggtcatagct 
     3901 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 
     3961 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 
     4021 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 
     4081 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 
     4141 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 
     4201 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 
     4261 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 
     4321 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 
     4381 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 
     4441 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 
     4501 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 
     4561 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 
     4621 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 
     4681 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacagta gaaggacagt 
     4741 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 
     4801 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 
     4861 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 
     4921 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 
     4981 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 
     5041 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 
     5101 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 
     5161 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 
     5221 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 
     5281 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 
     5341 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 
     5401 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 
     5461 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 
     5521 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 
     5581 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 
     5641 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 
     5701 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 
     5761 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 
     5821 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 
     5881 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 
     5941 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 
     6001 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 
     6061 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg 
     6121 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 
     6181 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 
     6241 gtgtcggggt tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 
     6301 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc 
     6361 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 
     6421 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca 
     6481 gtcacgacgt tgtaaaacga cggccagtgc caagttggga tctttgcatt ggcccacggc 
     6541 tctcaggatg gggatgctcc ccttcagcac ccggttcccc ttggaaactg atggtcctgg 
     6601 ctctgtggca tggcagtggc actgtgagga gcccctacca gcagcacaca gtgggtttgg 
     6661 cactgccacg ctccggatgc cgcgctctga tccaacccca taatcaaggg aacccgaatt 
     6721 gccccatcat tgcccccacc acccccatcc tgccgggccc tcacacccca cgctgccttg 
     6781 tggtgacatt ccccagccca aacccacggc ttcatggcta ccgcggggca tttcccattg 
     6841 ccgccccatt atcagctctg cacacctccc gctgtaccca tgcctcgtgg ctgcccttct 
     6901 ttgacgtata atcttctaat taatacccgg ccttgtcaaa gtggagcaca aacgttaatt 
     6961 aattccccag caggcaggta attaacagtg tgactccctt tttgctgcga gtggggctga 
     7021 tacagagaga tgtggcacta tggagcccac ggggtcctgg cactgggtgc ccacggaggt 
     7081 ccccatgtgc tgcagtgtca ccgcctccga ggtgacagta ttgtccctgc ggtgtccctg 
     7141 cagctcagct ctgtccacag ggccacctcc agtttggagg ggacacaatg cagccccgat 
     7201 gcaacccatc ctcgcagcat cccagggaca aagaccccac tgcaagaccg cacacagggc 
     7261 tgggtcccgc tcccctaata tctacagtgc ttttgcatgg ccccttaatc aatgcagtta 
     7321 atcagcatgc gctcatgcac cgctctggag ctgcaaagcc cctcgcagcg ctgctcacca 
     7381 acaccgcgca ccgccccggc ccagcctgca gcacgcgctg caaacaggaa agaaacaaaa 
     7441 tattgcccaa atgtaggcaa aggcattcgg ctgccttgac ctccgccggg ccgggccctg 
     7501 cctgactcag ctccttactc agcgctcgct tcctccctcc ggctgccacc gccgcagcgc 
     7561 acaccctgac aaagagtggc ccttaacggg ctctgaggtg cacccagcag tgcactcagc 
     7621 agtccaaggg ccggcctgga ggtttgcacc gctacgtgct gacattagca ttgaacttgg 
     7681 ccctgggtag tgctgcaggc cgggcggggt gggtgtagag agtgcagcgc gcgttgcacc 
     7741 cggtgcccct tcccctccct tgcatcccag caggctgcac cccagcacca ggcccgtgca 
     7801 tgcatgctcc tggtgttatt gcagcctggt gcatgcatgc gtcttagtgg tgcagcgctg 
     7861 tgcatgcatc ctccttggtg tgtagcagct tagtgcatgc atacccctcg gtgttattgc 
     7921 tgctctgtgc aggcacgctc attgtatcac ttcatcccag tgcatgcact cacactggag 
     7981 cgattgctgc tcggtgcacg cacactcatt gtatcacgtc agctcagtgg ctgcacgcac 
     8041 accggtgtta ttgctgctcg gtgcgtgcat gcacatcagt gtcgctgcag ctcagtgcat 
     8101 gcacgctcat tgcccatcgc tatccctgcc tctcctgctg gcgctccccg ggaggtgact 
     8161 tcaaggggac cgcaggacca cctcgggggt ggggggaggg ctgcacacgc ggaccccgct 
     8221 ccccctcccc aacaaagcac tgtggaatca aaaagggggg aggggggatg gaggggcgcg 
     8281 tcacaccccc gccccacacc ctcacctcga ggtgagcccc acgttctgct tcactctccc 
     8341 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc 
     8401 agcgatgggg gcgggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc 
     8461 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt 
     8521 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg 
     8581 cggg 
//
