LOCUS       pB80-KIF1a(1-365        8362 bp    DNA     circular     18-AUG-2021
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14004795|
COMMENT     VNTDBDATE|-14004795|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pB80-KIF1a(1-365)-GFP-SSPB(micro).gb|
COMMENT     VNTAUTHORNAME|Wilco Nijenhuis|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #2=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 6 4326 0 5572 0 6069 0 
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COMMENT        #3=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 1013 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 3 764 0 807 0 6131 0 0 "") 
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COMMENT        #9=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 4 1650 1 1713 1 2034 1 
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COMMENT        #11=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
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COMMENT        #13=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 3054 0 0 "") 
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COMMENT        #17=(CRSite (CStringList) "KasI" "ggcgcc" 2 0 3 760 0 803 0 6127 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "MfeI" "caattg" 2 0 2 1006 0 3508 0 0 "") 
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COMMENT        #25=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 1 6738 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "PstI" "ctgcag" 6 0 6 3052 0 6873 0 6921 0 
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COMMENT        #29=(CRSite (CStringList) "SacI" "gagctc" 6 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 368 0 6603 0 0 "") 
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COMMENT        #33=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") 
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COMMENT                          "Created: 08/18/21 12:30PM" 1) 
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COMMENT                          "Last Modified: 08/18/21 12:30PM" 1) 
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FEATURES             Location/Qualifiers
     CDS             2216..2929
                     /vntifkey="4"
                     /label=EGFP
     promoter        6232..8362
                     /vntifkey="29"
                     /label=B-actin\promoter
     CDS             4831..5691
                     /vntifkey="4"
                     /label=Amp\(r)
     primer_bind     3466..3489
                     /vntifkey="28"
                     /label=pB80\rev
     primer_bind     936..958
                     /vntifkey="28"
                     /label=pB80\fwd
     CDS             1022..2116
                     /vntifkey="4"
                     /label=KIF1A\(1-365)
     CDS             3029..3391
                     /vntifkey="4"
                     /label=SSPB(micro)
BASE COUNT     1796 a      2395 c      2435 g      1736 t 
ORIGIN
        1 agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc gcccgccccg 
       61 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg 
      121 ctgtaattag cgcttggttt aatgacggct cgtttctttt ctgtggctgc gtgaaagcct 
      181 taaagggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt 
      241 gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg agcgctgcgg 
      301 gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc cgggggcggt 
      361 gccccgcggt gcgggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg 
      421 gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta acccccccct gcacccccct 
      481 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg gcgtggcgcg 
      541 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg 
      601 cctcgggccg gggagggctc gggggagggg cgcggcggcc ccggagcgcc ggcggctgtc 
      661 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac 
      721 ttcctttgtc ccaaatctgg cggagccgaa atctgggagg cgccgccgca ccccctctag 
      781 cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt 
      841 gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgccg cagggggacg 
      901 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcgggg 
      961 tttatatctt cccttctctg ttcctccgca gcccccaagc taggcaattg ggcgcgccac 
     1021 catggccggg gcttcggtga aggtggcggt gcgtgtccgc cccttcaact cccgggaaat 
     1081 gagccgagac tccaagtgca tcattcagat gtctggaagc accaccacta ttgtcaaccc 
     1141 caaacagccc aaggagacac ccaagagctt cagctttgat tactcctact ggtcacatac 
     1201 ctcgccggag gacatcaact atgcctctca gaagcaggtc tacagggaca ttggggaaga 
     1261 gatgctacag catgcctttg agggctacaa tgtgtgcatc tttgcctatg ggcagacggg 
     1321 ggccggcaag tcctacacca tgatggggaa acaggagaag gaccagcaag gcatcattcc 
     1381 acagctctgc gaagacctct tctctcgaat caacgacacc accaacgaca acatgtccta 
     1441 ctctgtggag gtcagctaca tggagattta ctgtgagcga gtccgtgacc tcctgaaccc 
     1501 caagaacaaa ggcaacctgc gtgtgaggga gcaccctctg ctggggcctt atgtggagga 
     1561 cctctccaag ctggctgtaa cctcctacaa tgacatccag gacctcatgg actcagggaa 
     1621 caaggccagg actgtggcgg ccacaaacat gaatgagacc agcagccgtt ctcacgcagt 
     1681 cttcaacatc atctttaccc agaagcgcca tgatgcagag accaacatca ccacagagaa 
     1741 agtgagcaaa atcagcctgg tggacctggc aggcagtgaa cgagccgact ccacaggagc 
     1801 caagggcacg cgactcaagg agggggccaa catcaacaag tctttgacca ccctgggaaa 
     1861 ggtcatctct gctctggctg aaatggactc tggacccaac aagaacaaga aaaagaagaa 
     1921 gacagatttc atcccttacc gagactccgt attgacatgg cttctccggg aaaacctcgg 
     1981 tggcaactca aggacagcca tggtggcagc cctgagccct gcggacatca actatgatga 
     2041 gaccctcagc acactgagat atgcagatcg ggccaaacaa atccgctgca atgccatcat 
     2101 caatgaagac cccaactcta gaggtggcgg gggcagtggg ggagggggaa gtggaggcgg 
     2161 cggttctggc gggggaggct ccggtggcgg ggggagcggg ggtgggagta agcttgtgag 
     2221 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt 
     2281 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct 
     2341 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac 
     2401 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga 
     2461 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga 
     2521 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg 
     2581 cattgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga 
     2641 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa 
     2701 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta 
     2761 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag 
     2821 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga 
     2881 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg ctagcggagg 
     2941 cggtggctca ggcggaggag ggtcaggagg gggaggcagt ggcgggggcg gctctggagg 
     3001 agggggttct ggtggtggta gctacgtaga tctgagcggc ctgaccctgc aggaattcag 
     3061 ctccccgaaa cgccctaagc tgctgcgtga atattacgat tggctggttg ataacagctt 
     3121 taccccatat ctggtggtgg atgccacata cctgggcgtg aacgtgcccg tggagtatgt 
     3181 gaaagacggt cagatcgtgc tgaatctgtc tgcaagtgcg accggcaacc tgcaactgac 
     3241 aaatgatttt atccagttca acgcccagtt taagggcgtg tctcgtgaac tgtatatccc 
     3301 gatgggtgcc gctctggcca tttacgctcg cgagaacggc gatggtgtga tgttcgaacc 
     3361 agaagaaatc tatgacgagc tgaatattgg ttaaaccggt cctagataag taatgatcat 
     3421 aatcagccat atcacatctg tagaggtttt acttgcttta aaaaacctcc cacacctccc 
     3481 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta 
     3541 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 
     3601 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaattc 
     3661 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 
     3721 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 
     3781 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 
     3841 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 
     3901 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 
     3961 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 
     4021 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 
     4081 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 
     4141 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 
     4201 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 
     4261 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 
     4321 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 
     4381 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 
     4441 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 
     4501 ctacagtaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 
     4561 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 
     4621 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 
     4681 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 
     4741 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 
     4801 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 
     4861 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 
     4921 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 
     4981 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 
     5041 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 
     5101 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 
     5161 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 
     5221 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 
     5281 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 
     5341 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 
     5401 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 
     5461 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 
     5521 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 
     5581 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 
     5641 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 
     5701 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 
     5761 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 
     5821 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 
     5881 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 
     5941 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 
     6001 gtcagcgggt gttggcgggt gtcggggttg gcttaactat gcggcatcag agcagattgt 
     6061 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 
     6121 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 
     6181 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 
     6241 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agttgggatc 
     6301 tttgcattgg cccacggctc tcaggatggg gatgctcccc ttcagcaccc ggttcccctt 
     6361 ggaaactgat ggtcctggct ctgtggcatg gcagtggcac tgtgaggagc ccctaccagc 
     6421 agcacacagt gggtttggca ctgccacgct ccggatgccg cgctctgatc caaccccata 
     6481 atcaagggaa cccgaattgc cccatcattg cccccaccac ccccatcctg ccgggccctc 
     6541 acaccccacg ctgccttgtg gtgacattcc ccagcccaaa cccacggctt catggctacc 
     6601 gcggggcatt tcccattgcc gccccattat cagctctgca cacctcccgc tgtacccatg 
     6661 cctcgtggct gcccttcttt gacgtataat cttctaatta atacccggcc ttgtcaaagt 
     6721 ggagcacaaa cgttaattaa ttccccagca ggcaggtaat taacagtgtg actccctttt 
     6781 tgctgcgagt ggggctgata cagagagatg tggcactatg gagcccacgg ggtcctggca 
     6841 ctgggtgccc acggaggtcc ccatgtgctg cagtgtcacc gcctccgagg tgacagtatt 
     6901 gtccctgcgg tgtccctgca gctcagctct gtccacaggg ccacctccag tttggagggg 
     6961 acacaatgca gccccgatgc aacccatcct cgcagcatcc cagggacaaa gaccccactg 
     7021 caagaccgca cacagggctg ggtcccgctc ccctaatatc tacagtgctt ttgcatggcc 
     7081 ccttaatcaa tgcagttaat cagcatgcgc tcatgcaccg ctctggagct gcaaagcccc 
     7141 tcgcagcgct gctcaccaac accgcgcacc gccccggccc agcctgcagc acgcgctgca 
     7201 aacaggaaag aaacaaaata ttgcccaaat gtaggcaaag gcattcggct gccttgacct 
     7261 ccgccgggcc gggccctgcc tgactcagct ccttactcag cgctcgcttc ctccctccgg 
     7321 ctgccaccgc cgcagcgcac accctgacaa agagtggccc ttaacgggct ctgaggtgca 
     7381 cccagcagtg cactcagcag tccaagggcc ggcctggagg tttgcaccgc tacgtgctga 
     7441 cattagcatt gaacttggcc ctgggtagtg ctgcaggccg ggcggggtgg gtgtagagag 
     7501 tgcagcgcgc gttgcacccg gtgccccttc ccctcccttg catcccagca ggctgcaccc 
     7561 cagcaccagg cccgtgcatg catgctcctg gtgttattgc agcctggtgc atgcatgcgt 
     7621 cttagtggtg cagcgctgtg catgcatcct ccttggtgtg tagcagctta gtgcatgcat 
     7681 acccctcggt gttattgctg ctctgtgcag gcacgctcat tgtatcactt catcccagtg 
     7741 catgcactca cactggagcg attgctgctc ggtgcacgca cactcattgt atcacgtcag 
     7801 ctcagtggct gcacgcacac cggtgttatt gctgctcggt gcgtgcatgc acatcagtgt 
     7861 cgctgcagct cagtgcatgc acgctcattg cccatcgcta tccctgcctc tcctgctggc 
     7921 gctccccggg aggtgacttc aaggggaccg caggaccacc tcgggggtgg ggggagggct 
     7981 gcacacgcgg accccgctcc ccctccccaa caaagcactg tggaatcaaa aaggggggag 
     8041 gggggatgga ggggcgcgtc acacccccgc cccacaccct cacctcgagg tgagccccac 
     8101 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat 
     8161 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg 
     8221 cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga 
     8281 gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa 
     8341 agcgaagcgc gcggcgggcg gg 
//
