LOCUS       pB80-KIF1A(1-365        8767 bp    DNA     circular     23-AUG-2021
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14011798|
COMMENT     VNTDBDATE|-14011798|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro).gb|
COMMENT     VNTAUTHORNAME|Wilco Nijenhuis|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #5=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 1021 0 1999 0 0 "") 
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COMMENT        #26=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 3336 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 1 7143 0 0 "") 
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COMMENT        #30=(CRSite (CStringList) "SacI" "gagctc" 6 0 0 0 "") 
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FEATURES             Location/Qualifiers
     promoter        6637..8767
                     /vntifkey="29"
                     /label=B-actin\promoter
     CDS             5236..6096
                     /vntifkey="4"
                     /label=Amp\(r)
     primer_bind     3871..3894
                     /vntifkey="28"
                     /label=pB80\rev
     primer_bind     936..958
                     /vntifkey="28"
                     /label=pB80\fwd
     CDS             1022..2116
                     /vntifkey="4"
                     /label=KIF1A\(1-365)
     CDS             3434..3796
                     /vntifkey="4"
                     /label=SSPB(micro)
     CDS             2147..2590
                     /vntifkey="4"
                     /label=VVDfast
     CDS             2621..3334
                     /vntifkey="4"
                     /label=mVenus
BASE COUNT     1916 a      2494 c      2526 g      1831 t 
ORIGIN
        1 agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc gcccgccccg 
       61 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg 
      121 ctgtaattag cgcttggttt aatgacggct cgtttctttt ctgtggctgc gtgaaagcct 
      181 taaagggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt 
      241 gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg agcgctgcgg 
      301 gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc cgggggcggt 
      361 gccccgcggt gcgggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg 
      421 gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta acccccccct gcacccccct 
      481 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg gcgtggcgcg 
      541 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg 
      601 cctcgggccg gggagggctc gggggagggg cgcggcggcc ccggagcgcc ggcggctgtc 
      661 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac 
      721 ttcctttgtc ccaaatctgg cggagccgaa atctgggagg cgccgccgca ccccctctag 
      781 cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt 
      841 gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgccg cagggggacg 
      901 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcgggg 
      961 tttatatctt cccttctctg ttcctccgca gcccccaagc taggcaattg ggcgcgccac 
     1021 catggccggg gcttcggtga aggtggcggt gcgtgtccgc cccttcaact cccgggaaat 
     1081 gagccgagac tccaagtgca tcattcagat gtctggaagc accaccacta ttgtcaaccc 
     1141 caaacagccc aaggagacac ccaagagctt cagctttgat tactcctact ggtcacatac 
     1201 ctcgccggag gacatcaact atgcctctca gaagcaggtc tacagggaca ttggggaaga 
     1261 gatgctacag catgcctttg agggctacaa tgtgtgcatc tttgcctatg ggcagacggg 
     1321 ggccggcaag tcctacacca tgatggggaa acaggagaag gaccagcaag gcatcattcc 
     1381 acagctctgc gaagacctct tctctcgaat caacgacacc accaacgaca acatgtccta 
     1441 ctctgtggag gtcagctaca tggagattta ctgtgagcga gtccgtgacc tcctgaaccc 
     1501 caagaacaaa ggcaacctgc gtgtgaggga gcaccctctg ctggggcctt atgtggagga 
     1561 cctctccaag ctggctgtaa cctcctacaa tgacatccag gacctcatgg actcagggaa 
     1621 caaggccagg actgtggcgg ccacaaacat gaatgagacc agcagccgtt ctcacgcagt 
     1681 cttcaacatc atctttaccc agaagcgcca tgatgcagag accaacatca ccacagagaa 
     1741 agtgagcaaa atcagcctgg tggacctggc aggcagtgaa cgagccgact ccacaggagc 
     1801 caagggcacg cgactcaagg agggggccaa catcaacaag tctttgacca ccctgggaaa 
     1861 ggtcatctct gctctggctg aaatggactc tggacccaac aagaacaaga aaaagaagaa 
     1921 gacagatttc atcccttacc gagactccgt attgacatgg cttctccggg aaaacctcgg 
     1981 tggcaactca aggacagcca tggtggcagc cctgagccct gcggacatca actatgatga 
     2041 gaccctcagc acactgagat atgcagatcg ggccaaacaa atccgctgca atgccatcat 
     2101 caatgaagac cccaactcta gaggtggcgg gggcagtggg ggatcccata ctctttatgc 
     2161 ccccggtgga tatgacatta tgggatatct gtgccagatc atgaaccggc caaacccgca 
     2221 ggtggaactg ggccccgtgg atacatcctg cgccttggtt ctttgtgacc tgaaacagaa 
     2281 agacaccccg gtagtttacg cgagtgaagc cttcctctac atgacaggtt acagcaacgc 
     2341 agaggtgctg ggccggaatt gccggtttct gcaaagccct gacggcatgg tgaagcccaa 
     2401 gagcacccgg aagtacgtgg atagtaacac aatcaatact atgcgcaagg caatcgacag 
     2461 gaatgccgag gtgcaggttg aagtagtcaa ttttaaaaag aatggacagc gatttgttaa 
     2521 tttcctgact atgatacctg ttagggacga aacaggcgag tatcgatact ctatgggatt 
     2581 ccagtgcgaa gtcgacggga gcgggggtgg gagtaagctt gtgagcaagg gcgaggagct 
     2641 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt 
     2701 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttgat 
     2761 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tcggctacgg 
     2821 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc 
     2881 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 
     2941 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 
     3001 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag 
     3061 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggcaa acttcaagat 
     3121 ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc 
     3181 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaaact 
     3241 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 
     3301 cgggatcact ctcggcatgg acgagctgta caaggctagc ggaggcggtg gctcaggcgg 
     3361 aggagggtca ggagggggag gcagtggcgg gggcggctct ggaggagggg gttctggtgg 
     3421 tggtagctac gtagatctga gcggcctgac cctgcaggaa ttcagctccc cgaaacgccc 
     3481 taagctgctg cgtgaatatt acgattggct ggttgataac agctttaccc catatctggt 
     3541 ggtggatgcc acatacctgg gcgtgaacgt gcccgtggag tatgtgaaag acggtcagat 
     3601 cgtgctgaat ctgtctgcaa gtgcgaccgg caacctgcaa ctgacaaatg attttatcca 
     3661 gttcaacgcc cagtttaagg gcgtgtctcg tgaactgtat atcccgatgg gtgccgctct 
     3721 ggccatttac gctcgcgaga acggcgatgg tgtgatgttc gaaccagaag aaatctatga 
     3781 cgagctgaat attggttaaa ccggtcctag ataagtaatg atcataatca gccatatcac 
     3841 atctgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca 
     3901 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata 
     3961 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 
     4021 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aattcgtaat catggtcata 
     4081 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 
     4141 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 
     4201 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 
     4261 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 
     4321 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 
     4381 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 
     4441 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 
     4501 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 
     4561 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 
     4621 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 
     4681 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 
     4741 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 
     4801 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 
     4861 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca gtagaaggac 
     4921 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 
     4981 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 
     5041 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 
     5101 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 
     5161 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 
     5221 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 
     5281 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 
     5341 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 
     5401 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 
     5461 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 
     5521 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 
     5581 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 
     5641 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 
     5701 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 
     5761 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 
     5821 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 
     5881 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 
     5941 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 
     6001 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 
     6061 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 
     6121 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 
     6181 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 
     6241 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 
     6301 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 
     6361 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 
     6421 cgggtgtcgg ggttggctta actatgcggc atcagagcag attgtactga gagtgcacca 
     6481 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 
     6541 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 
     6601 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 
     6661 ccagtcacga cgttgtaaaa cgacggccag tgccaagttg ggatctttgc attggcccac 
     6721 ggctctcagg atggggatgc tccccttcag cacccggttc cccttggaaa ctgatggtcc 
     6781 tggctctgtg gcatggcagt ggcactgtga ggagccccta ccagcagcac acagtgggtt 
     6841 tggcactgcc acgctccgga tgccgcgctc tgatccaacc ccataatcaa gggaacccga 
     6901 attgccccat cattgccccc accaccccca tcctgccggg ccctcacacc ccacgctgcc 
     6961 ttgtggtgac attccccagc ccaaacccac ggcttcatgg ctaccgcggg gcatttccca 
     7021 ttgccgcccc attatcagct ctgcacacct cccgctgtac ccatgcctcg tggctgccct 
     7081 tctttgacgt ataatcttct aattaatacc cggccttgtc aaagtggagc acaaacgtta 
     7141 attaattccc cagcaggcag gtaattaaca gtgtgactcc ctttttgctg cgagtggggc 
     7201 tgatacagag agatgtggca ctatggagcc cacggggtcc tggcactggg tgcccacgga 
     7261 ggtccccatg tgctgcagtg tcaccgcctc cgaggtgaca gtattgtccc tgcggtgtcc 
     7321 ctgcagctca gctctgtcca cagggccacc tccagtttgg aggggacaca atgcagcccc 
     7381 gatgcaaccc atcctcgcag catcccaggg acaaagaccc cactgcaaga ccgcacacag 
     7441 ggctgggtcc cgctccccta atatctacag tgcttttgca tggcccctta atcaatgcag 
     7501 ttaatcagca tgcgctcatg caccgctctg gagctgcaaa gcccctcgca gcgctgctca 
     7561 ccaacaccgc gcaccgcccc ggcccagcct gcagcacgcg ctgcaaacag gaaagaaaca 
     7621 aaatattgcc caaatgtagg caaaggcatt cggctgcctt gacctccgcc gggccgggcc 
     7681 ctgcctgact cagctcctta ctcagcgctc gcttcctccc tccggctgcc accgccgcag 
     7741 cgcacaccct gacaaagagt ggcccttaac gggctctgag gtgcacccag cagtgcactc 
     7801 agcagtccaa gggccggcct ggaggtttgc accgctacgt gctgacatta gcattgaact 
     7861 tggccctggg tagtgctgca ggccgggcgg ggtgggtgta gagagtgcag cgcgcgttgc 
     7921 acccggtgcc ccttcccctc ccttgcatcc cagcaggctg caccccagca ccaggcccgt 
     7981 gcatgcatgc tcctggtgtt attgcagcct ggtgcatgca tgcgtcttag tggtgcagcg 
     8041 ctgtgcatgc atcctccttg gtgtgtagca gcttagtgca tgcatacccc tcggtgttat 
     8101 tgctgctctg tgcaggcacg ctcattgtat cacttcatcc cagtgcatgc actcacactg 
     8161 gagcgattgc tgctcggtgc acgcacactc attgtatcac gtcagctcag tggctgcacg 
     8221 cacaccggtg ttattgctgc tcggtgcgtg catgcacatc agtgtcgctg cagctcagtg 
     8281 catgcacgct cattgcccat cgctatccct gcctctcctg ctggcgctcc ccgggaggtg 
     8341 acttcaaggg gaccgcagga ccacctcggg ggtgggggga gggctgcaca cgcggacccc 
     8401 gctccccctc cccaacaaag cactgtggaa tcaaaaaggg gggagggggg atggaggggc 
     8461 gcgtcacacc cccgccccac accctcacct cgaggtgagc cccacgttct gcttcactct 
     8521 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg 
     8581 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg 
     8641 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa 
     8701 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc 
     8761 gggcggg 
//
