LOCUS       pB80-FRB-GFP-ppK        8614 bp    DNA     circular     23-AUG-2021
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14011799|
COMMENT     VNTDBDATE|-14011799|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pB80-FRB-GFP-ppKin14VIb.gb|
COMMENT     VNTAUTHORNAME|Wilco Nijenhuis|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     primer_bind     936..958
                     /vntifkey="28"
                     /label=pB80\fwd
     primer_bind     3718..3741
                     /vntifkey="28"
                     /label=pB80\rev
     CDS             5083..5943
                     /vntifkey="4"
                     /label=Amp\(r)
     promoter        6484..8614
                     /vntifkey="29"
                     /label=B-actin\promoter
     CDS             2222..3640
                     /vntifkey="4"
                     /label=Kin14\VIb\(Physcomitella\patens)
     CDS             1022..1303
                     /vntifkey="4"
                     /label=FRB
     CDS             1409..2122
                     /vntifkey="4"
                     /label=GFP
BASE COUNT     1941 a      2345 c      2484 g      1844 t 
ORIGIN
        1 agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc gcccgccccg 
       61 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg 
      121 ctgtaattag cgcttggttt aatgacggct cgtttctttt ctgtggctgc gtgaaagcct 
      181 taaagggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt 
      241 gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg agcgctgcgg 
      301 gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc cgggggcggt 
      361 gccccgcggt gcgggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg 
      421 gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta acccccccct gcacccccct 
      481 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg gcgtggcgcg 
      541 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg 
      601 cctcgggccg gggagggctc gggggagggg cgcggcggcc ccggagcgcc ggcggctgtc 
      661 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac 
      721 ttcctttgtc ccaaatctgg cggagccgaa atctgggagg cgccgccgca ccccctctag 
      781 cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt 
      841 gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgccg cagggggacg 
      901 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcgggg 
      961 tttatatctt cccttctctg ttcctccgca gcccccaagc taggcaattg ggcgcgccac 
     1021 catgatcctc tggcatgaga tgtggcatga aggcctggaa gaggcatctc gtttgtactt 
     1081 tggggaaagg aacgtgaaag gcatgtttga ggtgctggag cccttgcatg ctatgatgga 
     1141 acggggcccc cagactctga aggaaacatc ctttaatcag gcctatggtc gagatttaat 
     1201 ggaggcccaa gagtggtgca ggaagtacat gaaatcaggg aatgtcaagg acctcctcca 
     1261 agcctgggac ctctattatc atgtgttccg acgaatctca aagcaattgt ctagaggtgg 
     1321 cgggggcagt gggggagggg gaagtggagg cggcggttct ggcgggggag gctccggtgg 
     1381 cggggggagc gggggtggga gtaagcttgt gagcaagggc gaggagctgt tcaccggggt 
     1441 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 
     1501 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 
     1561 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt 
     1621 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 
     1681 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 
     1741 ggtgaagttc gagggcgaca ccctggtgaa ccgcattgag ctgaagggca tcgacttcaa 
     1801 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta 
     1861 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat 
     1921 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 
     1981 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc 
     2041 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 
     2101 cggcatggac gagctgtaca aggctagcgg aggcggtggc tcaggcggag gagggtcagg 
     2161 agggggaggc agtggcgggg gcggctctgg aggagggggt tctggtggtg gtagctacgt 
     2221 aagagatgat ttgaggggac gacttgtaac tctcgagaag aaattagcag agcgtaccca 
     2281 agatttgagt cttgcagagt ctactttggc aactcgtgag ggtgaattcg aatccctgca 
     2341 aagcaatcta atggagctta atgagctccg ggagatgaag gaggatatcg acagaaagaa 
     2401 tgagcaaaca gcagctatat tgaagcgaca agcagaccag ataactgaat tagaaactct 
     2461 ttatagggaa gaacaagtcc ttcgaaaacg atacttcaac atgatggaag acatgaaagg 
     2521 taagatcagg gtatatgctc ggtggagacc cctaagtagt aaggaggtca aagagcggca 
     2581 acaaaacgtg ttgatagcac ctgatgaatt caccatcgaa catccttgga aagacgacaa 
     2641 acccaaacaa catcaatttg atcacgtttt cgaccaccat gcgacccaag aagaggtctt 
     2701 tgaggataca aagtatttgg tccaatcagc aattgacgga tacaatgtct gtatatttgc 
     2761 atacgggcaa actgggtcag gcaaaacgtt tacgatttac ggttctgaca acaacccagg 
     2821 tctaactcca cgagctacca aagaactttt tggctatctt aagcgagacg ccaataagtt 
     2881 ttcgtttgct ctgaaggttt acatgcttga aatatatcaa gattcgctca tcgatctgct 
     2941 tctaccaaag agtgctgcaa agccacgaaa gcttgagatt aagaaggact ccaagggaat 
     3001 ggttgtcgta gagaatgcaa ctctactgcc gattgcatcc catgatgaac tgcaagccat 
     3061 tgttcataag ggtctggaga gacgacatgt ttcgggcacc cacatgaatg cggagagttc 
     3121 acggtcacac ctcattctat cagtaatcgt tgaaagcacc aaccgacagt ctcaagttct 
     3181 tgtgaagggc aagctgagtt ttgtggatct tgcgggctct gaaagagtga agaaatctgg 
     3241 atccagtggt gaacaactga aagaggccca aagcattaat aaatctttgt cagcactagg 
     3301 tgatgtcatt agcgcccttg caacagagga gcagcatatt ccttaccgta accacaagct 
     3361 aacaatgctt atgagcgatt ctctcggtgg aaatgcgaag accctcatgt ttgtaaatat 
     3421 ctcaccagca gagtctaatc tggacgagac ccataattca ctctgctatg ccacaagggt 
     3481 aagatccatc atcaatgagg ccagtaaaaa tactacaacg aaagagatac tgcgactgaa 
     3541 gaaacaaatc gcgtactgga aggagcaagc gggcaggatg ggagggtccg aggatctgga 
     3601 agaggttatg gatgatcgtg gaatcaaaac tgcagattaa acgcgtaccg gtcctagata 
     3661 agtaatgatc ataatcagcc atatcacatc tgtagaggtt ttacttgctt taaaaaacct 
     3721 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt 
     3781 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 
     3841 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 
     3901 ctggatcaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 
     3961 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 
     4021 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 
     4081 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 
     4141 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     4201 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     4261 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     4321 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     4381 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     4441 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     4501 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     4561 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     4621 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     4681 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     4741 gcctaactac ggctacagta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 
     4801 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     4861 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
     4921 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 
     4981 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 
     5041 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 
     5101 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 
     5161 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 
     5221 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 
     5281 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 
     5341 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 
     5401 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 
     5461 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 
     5521 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 
     5581 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 
     5641 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 
     5701 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 
     5761 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 
     5821 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 
     5881 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 
     5941 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 
     6001 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 
     6061 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 
     6121 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 
     6181 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 
     6241 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggt tggcttaact atgcggcatc 
     6301 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 
     6361 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 
     6421 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 
     6481 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 
     6541 caagttggga tctttgcatt ggcccacggc tctcaggatg gggatgctcc ccttcagcac 
     6601 ccggttcccc ttggaaactg atggtcctgg ctctgtggca tggcagtggc actgtgagga 
     6661 gcccctacca gcagcacaca gtgggtttgg cactgccacg ctccggatgc cgcgctctga 
     6721 tccaacccca taatcaaggg aacccgaatt gccccatcat tgcccccacc acccccatcc 
     6781 tgccgggccc tcacacccca cgctgccttg tggtgacatt ccccagccca aacccacggc 
     6841 ttcatggcta ccgcggggca tttcccattg ccgccccatt atcagctctg cacacctccc 
     6901 gctgtaccca tgcctcgtgg ctgcccttct ttgacgtata atcttctaat taatacccgg 
     6961 ccttgtcaaa gtggagcaca aacgttaatt aattccccag caggcaggta attaacagtg 
     7021 tgactccctt tttgctgcga gtggggctga tacagagaga tgtggcacta tggagcccac 
     7081 ggggtcctgg cactgggtgc ccacggaggt ccccatgtgc tgcagtgtca ccgcctccga 
     7141 ggtgacagta ttgtccctgc ggtgtccctg cagctcagct ctgtccacag ggccacctcc 
     7201 agtttggagg ggacacaatg cagccccgat gcaacccatc ctcgcagcat cccagggaca 
     7261 aagaccccac tgcaagaccg cacacagggc tgggtcccgc tcccctaata tctacagtgc 
     7321 ttttgcatgg ccccttaatc aatgcagtta atcagcatgc gctcatgcac cgctctggag 
     7381 ctgcaaagcc cctcgcagcg ctgctcacca acaccgcgca ccgccccggc ccagcctgca 
     7441 gcacgcgctg caaacaggaa agaaacaaaa tattgcccaa atgtaggcaa aggcattcgg 
     7501 ctgccttgac ctccgccggg ccgggccctg cctgactcag ctccttactc agcgctcgct 
     7561 tcctccctcc ggctgccacc gccgcagcgc acaccctgac aaagagtggc ccttaacggg 
     7621 ctctgaggtg cacccagcag tgcactcagc agtccaaggg ccggcctgga ggtttgcacc 
     7681 gctacgtgct gacattagca ttgaacttgg ccctgggtag tgctgcaggc cgggcggggt 
     7741 gggtgtagag agtgcagcgc gcgttgcacc cggtgcccct tcccctccct tgcatcccag 
     7801 caggctgcac cccagcacca ggcccgtgca tgcatgctcc tggtgttatt gcagcctggt 
     7861 gcatgcatgc gtcttagtgg tgcagcgctg tgcatgcatc ctccttggtg tgtagcagct 
     7921 tagtgcatgc atacccctcg gtgttattgc tgctctgtgc aggcacgctc attgtatcac 
     7981 ttcatcccag tgcatgcact cacactggag cgattgctgc tcggtgcacg cacactcatt 
     8041 gtatcacgtc agctcagtgg ctgcacgcac accggtgtta ttgctgctcg gtgcgtgcat 
     8101 gcacatcagt gtcgctgcag ctcagtgcat gcacgctcat tgcccatcgc tatccctgcc 
     8161 tctcctgctg gcgctccccg ggaggtgact tcaaggggac cgcaggacca cctcgggggt 
     8221 ggggggaggg ctgcacacgc ggaccccgct ccccctcccc aacaaagcac tgtggaatca 
     8281 aaaagggggg aggggggatg gaggggcgcg tcacaccccc gccccacacc ctcacctcga 
     8341 ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt 
     8401 gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggcgcg 
     8461 cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg 
     8521 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc 
     8581 ggccctataa aaagcgaagc gcgcggcggg cggg 
//
