LOCUS       pWN277_pB80-SSPB        8698 bp    DNA     circular      9-MAR-2020
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13246256|
COMMENT     VNTDBDATE|-13246256|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pWN277_pB80-SSPB(micro)-Venus-ppKin14VIb.gb|
COMMENT     VNTAUTHORNAME|Wilco Nijenhuis|
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FEATURES             Location/Qualifiers
     primer_bind     936..958
                     /vntifkey="28"
                     /label=pB80\fwd
     primer_bind     3802..3825
                     /vntifkey="28"
                     /label=pB80\rev
     CDS             5167..6027
                     /vntifkey="4"
                     /label=Amp\(r)
     promoter        6568..8698
                     /vntifkey="29"
                     /label=B-actin\promoter
     CDS             2306..3724
                     /vntifkey="4"
                     /label=ppKin14VIB\(861-1321)
     CDS             1022..1393
                     /vntifkey="4"
                     /label=SSPB(micro)
     CDS             1493..2206
                     /vntifkey="4"
                     /label=mVenus
BASE COUNT     1953 a      2375 c      2503 g      1867 t 
ORIGIN
        1 agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc gcccgccccg 
       61 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg 
      121 ctgtaattag cgcttggttt aatgacggct cgtttctttt ctgtggctgc gtgaaagcct 
      181 taaagggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt 
      241 gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg agcgctgcgg 
      301 gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc cgggggcggt 
      361 gccccgcggt gcgggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg 
      421 gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta acccccccct gcacccccct 
      481 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg gcgtggcgcg 
      541 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg 
      601 cctcgggccg gggagggctc gggggagggg cgcggcggcc ccggagcgcc ggcggctgtc 
      661 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac 
      721 ttcctttgtc ccaaatctgg cggagccgaa atctgggagg cgccgccgca ccccctctag 
      781 cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt 
      841 gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgccg cagggggacg 
      901 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcgggg 
      961 tttatatctt cccttctctg ttcctccgca gcccccaagc taggcaattg ggcgcgccac 
     1021 catggatctg agcggcctga ccctgcagga attcagctcc ccgaaacgcc ctaagctgct 
     1081 gcgtgaatat tacgattggc tggttgataa cagctttacc ccatatctgg tggtggatgc 
     1141 cacatacctg ggcgtgaacg tgcccgtgga gtatgtgaaa gacggtcaga tcgtgctgaa 
     1201 tctgtctgca agtgcgaccg gcaacctgca actgacaaat gattttatcc agttcaacgc 
     1261 ccagtttaag ggcgtgtctc gtgaactgta tatcccgatg ggtgccgctc tggccattta 
     1321 cgctcgcgag aacggcgatg gtgtgatgtt cgaaccagaa gaaatctatg acgagctgaa 
     1381 tattggtcaa ttgtctagag gtggcggggg cagtggggga gggggaagtg gaggcggcgg 
     1441 ttctggcggg ggaggctccg gtggcggggg gagcgggggt gggagtaagc ttgtgagcaa 
     1501 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 
     1561 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac 
     1621 cctgaagttg atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 
     1681 cctcggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt 
     1741 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga 
     1801 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat 
     1861 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 
     1921 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggc 
     1981 aaacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgccg accactacca 
     2041 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta 
     2101 ccagtccaaa ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 
     2161 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggcta gcggaggcgg 
     2221 tggctcaggc ggaggagggt caggaggggg aggcagtggc gggggcggct ctggaggagg 
     2281 gggttctggt ggtggtagct acgtaagaga tgatttgagg ggacgacttg taactctcga 
     2341 gaagaaatta gcagagcgta cccaagattt gagtcttgca gagtctactt tggcaactcg 
     2401 tgagggtgaa ttcgaatccc tgcaaagcaa tctaatggag cttaatgagc tccgggagat 
     2461 gaaggaggat atcgacagaa agaatgagca aacagcagct atattgaagc gacaagcaga 
     2521 ccagataact gaattagaaa ctctttatag ggaagaacaa gtccttcgaa aacgatactt 
     2581 caacatgatg gaagacatga aaggtaagat cagggtatat gctcggtgga gacccctaag 
     2641 tagtaaggag gtcaaagagc ggcaacaaaa cgtgttgata gcacctgatg aattcaccat 
     2701 cgaacatcct tggaaagacg acaaacccaa acaacatcaa tttgatcacg ttttcgacca 
     2761 ccatgcgacc caagaagagg tctttgagga tacaaagtat ttggtccaat cagcaattga 
     2821 cggatacaat gtctgtatat ttgcatacgg gcaaactggg tcaggcaaaa cgtttacgat 
     2881 ttacggttct gacaacaacc caggtctaac tccacgagct accaaagaac tttttggcta 
     2941 tcttaagcga gacgccaata agttttcgtt tgctctgaag gtttacatgc ttgaaatata 
     3001 tcaagattcg ctcatcgatc tgcttctacc aaagagtgct gcaaagccac gaaagcttga 
     3061 gattaagaag gactccaagg gaatggttgt cgtagagaat gcaactctac tgccgattgc 
     3121 atcccatgat gaactgcaag ccattgttca taagggtctg gagagacgac atgtttcggg 
     3181 cacccacatg aatgcggaga gttcacggtc acacctcatt ctatcagtaa tcgttgaaag 
     3241 caccaaccga cagtctcaag ttcttgtgaa gggcaagctg agttttgtgg atcttgcggg 
     3301 ctctgaaaga gtgaagaaat ctggatccag tggtgaacaa ctgaaagagg cccaaagcat 
     3361 taataaatct ttgtcagcac taggtgatgt cattagcgcc cttgcaacag aggagcagca 
     3421 tattccttac cgtaaccaca agctaacaat gcttatgagc gattctctcg gtggaaatgc 
     3481 gaagaccctc atgtttgtaa atatctcacc agcagagtct aatctggacg agacccataa 
     3541 ttcactctgc tatgccacaa gggtaagatc catcatcaat gaggccagta aaaatactac 
     3601 aacgaaagag atactgcgac tgaagaaaca aatcgcgtac tggaaggagc aagcgggcag 
     3661 gatgggaggg tccgaggatc tggaagaggt tatggatgat cgtggaatca aaactgcaga 
     3721 ttaaacgcgt accggtccta gataagtaat gatcataatc agccatatca catctgtaga 
     3781 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa 
     3841 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag 
     3901 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 
     3961 actcatcaat gtatcttatc atgtctggat caattcgtaa tcatggtcat agctgtttcc 
     4021 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 
     4081 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 
     4141 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 
     4201 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
     4261 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
     4321 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 
     4381 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
     4441 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
     4501 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
     4561 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 
     4621 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
     4681 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
     4741 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
     4801 tgctacagag ttcttgaagt ggtggcctaa ctacggctac agtagaagga cagtatttgg 
     4861 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
     4921 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 
     4981 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 
     5041 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 
     5101 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 
     5161 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 
     5221 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 
     5281 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 
     5341 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 
     5401 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 
     5461 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 
     5521 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 
     5581 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 
     5641 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 
     5701 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 
     5761 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 
     5821 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 
     5881 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 
     5941 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 
     6001 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 
     6061 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 
     6121 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 
     6181 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg 
     6241 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 
     6301 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 
     6361 gggttggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 
     6421 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag 
     6481 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 
     6541 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 
     6601 acgttgtaaa acgacggcca gtgccaagtt gggatctttg cattggccca cggctctcag 
     6661 gatggggatg ctccccttca gcacccggtt ccccttggaa actgatggtc ctggctctgt 
     6721 ggcatggcag tggcactgtg aggagcccct accagcagca cacagtgggt ttggcactgc 
     6781 cacgctccgg atgccgcgct ctgatccaac cccataatca agggaacccg aattgcccca 
     6841 tcattgcccc caccaccccc atcctgccgg gccctcacac cccacgctgc cttgtggtga 
     6901 cattccccag cccaaaccca cggcttcatg gctaccgcgg ggcatttccc attgccgccc 
     6961 cattatcagc tctgcacacc tcccgctgta cccatgcctc gtggctgccc ttctttgacg 
     7021 tataatcttc taattaatac ccggccttgt caaagtggag cacaaacgtt aattaattcc 
     7081 ccagcaggca ggtaattaac agtgtgactc cctttttgct gcgagtgggg ctgatacaga 
     7141 gagatgtggc actatggagc ccacggggtc ctggcactgg gtgcccacgg aggtccccat 
     7201 gtgctgcagt gtcaccgcct ccgaggtgac agtattgtcc ctgcggtgtc cctgcagctc 
     7261 agctctgtcc acagggccac ctccagtttg gaggggacac aatgcagccc cgatgcaacc 
     7321 catcctcgca gcatcccagg gacaaagacc ccactgcaag accgcacaca gggctgggtc 
     7381 ccgctcccct aatatctaca gtgcttttgc atggcccctt aatcaatgca gttaatcagc 
     7441 atgcgctcat gcaccgctct ggagctgcaa agcccctcgc agcgctgctc accaacaccg 
     7501 cgcaccgccc cggcccagcc tgcagcacgc gctgcaaaca ggaaagaaac aaaatattgc 
     7561 ccaaatgtag gcaaaggcat tcggctgcct tgacctccgc cgggccgggc cctgcctgac 
     7621 tcagctcctt actcagcgct cgcttcctcc ctccggctgc caccgccgca gcgcacaccc 
     7681 tgacaaagag tggcccttaa cgggctctga ggtgcaccca gcagtgcact cagcagtcca 
     7741 agggccggcc tggaggtttg caccgctacg tgctgacatt agcattgaac ttggccctgg 
     7801 gtagtgctgc aggccgggcg gggtgggtgt agagagtgca gcgcgcgttg cacccggtgc 
     7861 cccttcccct cccttgcatc ccagcaggct gcaccccagc accaggcccg tgcatgcatg 
     7921 ctcctggtgt tattgcagcc tggtgcatgc atgcgtctta gtggtgcagc gctgtgcatg 
     7981 catcctcctt ggtgtgtagc agcttagtgc atgcataccc ctcggtgtta ttgctgctct 
     8041 gtgcaggcac gctcattgta tcacttcatc ccagtgcatg cactcacact ggagcgattg 
     8101 ctgctcggtg cacgcacact cattgtatca cgtcagctca gtggctgcac gcacaccggt 
     8161 gttattgctg ctcggtgcgt gcatgcacat cagtgtcgct gcagctcagt gcatgcacgc 
     8221 tcattgccca tcgctatccc tgcctctcct gctggcgctc cccgggaggt gacttcaagg 
     8281 ggaccgcagg accacctcgg gggtgggggg agggctgcac acgcggaccc cgctccccct 
     8341 ccccaacaaa gcactgtgga atcaaaaagg ggggaggggg gatggagggg cgcgtcacac 
     8401 ccccgcccca caccctcacc tcgaggtgag ccccacgttc tgcttcactc tccccatctc 
     8461 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat 
     8521 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg 
     8581 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt 
     8641 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcggg 
//
