LOCUS       pB80-SSPB(nano)-        8758 bp    DNA     circular     23-AUG-2021
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14011804|
COMMENT     VNTDBDATE|-14011804|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pB80-SSPB(nano)-GFP-GCN4-ppKin14VIb.gb|
COMMENT     VNTAUTHORNAME|Wilco Nijenhuis|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #5=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 3384 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 3 764 0 807 0 6527 0 0 "") 
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COMMENT        #9=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 3 2684 1 3585 1 5362 1 0 
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COMMENT        #12=(CRSite (CStringList) "EagI" "cggccg" 2 0 2 348 0 2359 0 0 "") 
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COMMENT        #18=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 0 0 "") 
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COMMENT        #26=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 1 7134 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 0 0 "") 
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COMMENT        #29=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 2512 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 368 0 6999 0 0 "") 
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COMMENT        #38=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 8322 0 0 "")) 
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COMMENT        #43=(CFSignal (CObList) "EGFP" 4 0 0 1493 2206 0 (CStringList) 
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COMMENT        #44=(CFSignal (CObList) "SSPB(nano)" 4 0 0 1022 1387 0 (CStringList) 
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COMMENT        #45=(CFSignal (CObList) "GCN4" 4 0 0 2246 2332 0 (CStringList) 
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COMMENT                          "Created: 08/23/21 09:19AM" 1) 
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COMMENT                          "Last Modified: 08/23/21 09:19AM" 1) 
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FEATURES             Location/Qualifiers
     primer_bind     936..958
                     /vntifkey="28"
                     /label=pB80\fwd
     primer_bind     3862..3885
                     /vntifkey="28"
                     /label=pB80\rev
     CDS             5227..6087
                     /vntifkey="4"
                     /label=Amp\(r)
     promoter        6628..8758
                     /vntifkey="29"
                     /label=B-actin\promoter
     CDS             1493..2206
                     /vntifkey="4"
                     /label=EGFP
     CDS             1022..1387
                     /vntifkey="4"
                     /label=SSPB(nano)
     CDS             2246..2332
                     /vntifkey="4"
                     /label=GCN4
     CDS             2366..3784
                     /vntifkey="4"
                     /label=ppKin14VIB\(861-1321)
BASE COUNT     1989 a      2386 c      2493 g      1890 t 
ORIGIN
        1 agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc gcccgccccg 
       61 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg 
      121 ctgtaattag cgcttggttt aatgacggct cgtttctttt ctgtggctgc gtgaaagcct 
      181 taaagggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt 
      241 gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg agcgctgcgg 
      301 gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc cgggggcggt 
      361 gccccgcggt gcgggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg 
      421 gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta acccccccct gcacccccct 
      481 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg gcgtggcgcg 
      541 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg 
      601 cctcgggccg gggagggctc gggggagggg cgcggcggcc ccggagcgcc ggcggctgtc 
      661 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac 
      721 ttcctttgtc ccaaatctgg cggagccgaa atctgggagg cgccgccgca ccccctctag 
      781 cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt 
      841 gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgccg cagggggacg 
      901 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcgggg 
      961 tttatatctt cccttctctg ttcctccgca gcccccaagc taggcaattg ggcgcgccac 
     1021 catggatctg agcggcctga ccctgcagga attcagctcc ccgaaacgcc ctaagctgct 
     1081 gcgtgaatat tacgattggc tggttgataa cagctttacc ccatatctgg tggtggatgc 
     1141 cacatacctg ggcgtgaacg tgcccgtgga gtatgtgaaa gacggtcaga tcgtgctgaa 
     1201 tctgtctgca agtgcgaccg gcaacctgca actgacaaat gattttatcc agttcaacgc 
     1261 ccgctttaag ggcgtgtctc gtgaactgta tatcccgatg ggtgccgctc tggccattta 
     1321 cgctcgcgag aacggcgatg gtgtgatgtt cgaaccagaa gaaatctatg acgagctgaa 
     1381 tattggtcaa ttgtctagag gtggcggggg cagtggggga gggggaagtg gaggcggcgg 
     1441 ttctggcggg ggaggctccg gtggcggggg gagcgggggt gggagtaagc ttgtgagcaa 
     1501 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 
     1561 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac 
     1621 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 
     1681 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt 
     1741 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga 
     1801 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat 
     1861 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 
     1921 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt 
     1981 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca 
     2041 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac 
     2101 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 
     2161 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagcacc ggtatacaag 
     2221 tttgtacaaa aaagcaggct ccgctattga agacaagttg gaagaaatac tttcgaaatt 
     2281 gtaccacatt gaaaatgagt tggccagaat taagaaatta ttgggcgaaa ttggtggagg 
     2341 tagtggtgga ggtagtgcgg ccgccagaga tgatttgagg ggacgacttg taactctcga 
     2401 gaagaaatta gcagagcgta cccaagattt gagtcttgca gagtctactt tggcaactcg 
     2461 tgagggtgaa ttcgaatccc tgcaaagcaa tctaatggag cttaatgagc tccgggagat 
     2521 gaaggaggat atcgacagaa agaatgagca aacagcagct atattgaagc gacaagcaga 
     2581 ccagataact gaattagaaa ctctttatag ggaagaacaa gtccttcgaa aacgatactt 
     2641 caacatgatg gaagacatga aaggtaagat cagggtatat gctcggtgga gacccctaag 
     2701 tagtaaggag gtcaaagagc ggcaacaaaa cgtgttgata gcacctgatg aattcaccat 
     2761 cgaacatcct tggaaagacg acaaacccaa acaacatcaa tttgatcacg ttttcgacca 
     2821 ccatgcgacc caagaagagg tctttgagga tacaaagtat ttggtccaat cagcaattga 
     2881 cggatacaat gtctgtatat ttgcatacgg gcaaactggg tcaggcaaaa cgtttacgat 
     2941 ttacggttct gacaacaacc caggtctaac tccacgagct accaaagaac tttttggcta 
     3001 tcttaagcga gacgccaata agttttcgtt tgctctgaag gtttacatgc ttgaaatata 
     3061 tcaagattcg ctcatcgatc tgcttctacc aaagagtgct gcaaagccac gaaagcttga 
     3121 gattaagaag gactccaagg gaatggttgt cgtagagaat gcaactctac tgccgattgc 
     3181 atcccatgat gaactgcaag ccattgttca taagggtctg gagagacgac atgtttcggg 
     3241 cacccacatg aatgcggaga gttcacggtc acacctcatt ctatcagtaa tcgttgaaag 
     3301 caccaaccga cagtctcaag ttcttgtgaa gggcaagctg agttttgtgg atcttgcggg 
     3361 ctctgaaaga gtgaagaaat ctggatccag tggtgaacaa ctgaaagagg cccaaagcat 
     3421 taataaatct ttgtcagcac taggtgatgt cattagcgcc cttgcaacag aggagcagca 
     3481 tattccttac cgtaaccaca agctaacaat gcttatgagc gattctctcg gtggaaatgc 
     3541 gaagaccctc atgtttgtaa atatctcacc agcagagtct aatctggacg agacccataa 
     3601 ttcactctgc tatgccacaa gggtaagatc catcatcaat gaggccagta aaaatactac 
     3661 aacgaaagag atactgcgac tgaagaaaca aatcgcgtac tggaaggagc aagcgggcag 
     3721 gatgggaggg tccgaggatc tggaagaggt tatggatgat cgtggaatca aaactgcaga 
     3781 ttaaacgcgt accggtccta gataagtaat gatcataatc agccatatca catctgtaga 
     3841 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa 
     3901 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag 
     3961 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 
     4021 actcatcaat gtatcttatc atgtctggat caattcgtaa tcatggtcat agctgtttcc 
     4081 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 
     4141 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 
     4201 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 
     4261 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
     4321 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
     4381 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 
     4441 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
     4501 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
     4561 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
     4621 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 
     4681 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
     4741 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
     4801 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
     4861 tgctacagag ttcttgaagt ggtggcctaa ctacggctac agtagaagga cagtatttgg 
     4921 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
     4981 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 
     5041 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 
     5101 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 
     5161 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 
     5221 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 
     5281 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 
     5341 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 
     5401 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 
     5461 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 
     5521 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 
     5581 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 
     5641 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 
     5701 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 
     5761 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 
     5821 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 
     5881 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 
     5941 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 
     6001 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 
     6061 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 
     6121 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 
     6181 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 
     6241 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg 
     6301 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 
     6361 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 
     6421 gggttggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 
     6481 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag 
     6541 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 
     6601 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 
     6661 acgttgtaaa acgacggcca gtgccaagtt gggatctttg cattggccca cggctctcag 
     6721 gatggggatg ctccccttca gcacccggtt ccccttggaa actgatggtc ctggctctgt 
     6781 ggcatggcag tggcactgtg aggagcccct accagcagca cacagtgggt ttggcactgc 
     6841 cacgctccgg atgccgcgct ctgatccaac cccataatca agggaacccg aattgcccca 
     6901 tcattgcccc caccaccccc atcctgccgg gccctcacac cccacgctgc cttgtggtga 
     6961 cattccccag cccaaaccca cggcttcatg gctaccgcgg ggcatttccc attgccgccc 
     7021 cattatcagc tctgcacacc tcccgctgta cccatgcctc gtggctgccc ttctttgacg 
     7081 tataatcttc taattaatac ccggccttgt caaagtggag cacaaacgtt aattaattcc 
     7141 ccagcaggca ggtaattaac agtgtgactc cctttttgct gcgagtgggg ctgatacaga 
     7201 gagatgtggc actatggagc ccacggggtc ctggcactgg gtgcccacgg aggtccccat 
     7261 gtgctgcagt gtcaccgcct ccgaggtgac agtattgtcc ctgcggtgtc cctgcagctc 
     7321 agctctgtcc acagggccac ctccagtttg gaggggacac aatgcagccc cgatgcaacc 
     7381 catcctcgca gcatcccagg gacaaagacc ccactgcaag accgcacaca gggctgggtc 
     7441 ccgctcccct aatatctaca gtgcttttgc atggcccctt aatcaatgca gttaatcagc 
     7501 atgcgctcat gcaccgctct ggagctgcaa agcccctcgc agcgctgctc accaacaccg 
     7561 cgcaccgccc cggcccagcc tgcagcacgc gctgcaaaca ggaaagaaac aaaatattgc 
     7621 ccaaatgtag gcaaaggcat tcggctgcct tgacctccgc cgggccgggc cctgcctgac 
     7681 tcagctcctt actcagcgct cgcttcctcc ctccggctgc caccgccgca gcgcacaccc 
     7741 tgacaaagag tggcccttaa cgggctctga ggtgcaccca gcagtgcact cagcagtcca 
     7801 agggccggcc tggaggtttg caccgctacg tgctgacatt agcattgaac ttggccctgg 
     7861 gtagtgctgc aggccgggcg gggtgggtgt agagagtgca gcgcgcgttg cacccggtgc 
     7921 cccttcccct cccttgcatc ccagcaggct gcaccccagc accaggcccg tgcatgcatg 
     7981 ctcctggtgt tattgcagcc tggtgcatgc atgcgtctta gtggtgcagc gctgtgcatg 
     8041 catcctcctt ggtgtgtagc agcttagtgc atgcataccc ctcggtgtta ttgctgctct 
     8101 gtgcaggcac gctcattgta tcacttcatc ccagtgcatg cactcacact ggagcgattg 
     8161 ctgctcggtg cacgcacact cattgtatca cgtcagctca gtggctgcac gcacaccggt 
     8221 gttattgctg ctcggtgcgt gcatgcacat cagtgtcgct gcagctcagt gcatgcacgc 
     8281 tcattgccca tcgctatccc tgcctctcct gctggcgctc cccgggaggt gacttcaagg 
     8341 ggaccgcagg accacctcgg gggtgggggg agggctgcac acgcggaccc cgctccccct 
     8401 ccccaacaaa gcactgtgga atcaaaaagg ggggaggggg gatggagggg cgcgtcacac 
     8461 ccccgcccca caccctcacc tcgaggtgag ccccacgttc tgcttcactc tccccatctc 
     8521 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat 
     8581 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg 
     8641 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt 
     8701 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcggg 
//
