LOCUS       pBA-mKeima-PIM          8825 bp    DNA     circular     18-AUG-2021
DEFINITION  .
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14004743|
COMMENT     VNTDBDATE|-14004743|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBA-mKeima-PIM|
COMMENT     VNTAUTHORNAME|Wilco Nijenhuis|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT         "BglII" "BsaI" "BspEI" "ClaI" "EagI" "EcoRI" "EcoRV" "HindIII" "HpaI" 
COMMENT         "KasI" "KpnI" "MfeI" "MluI" "NarI" "NcoI" "NdeI" "NheI" "NotI" "PacI" 
COMMENT         "PmeI" "PstI" "SacI" "SacII" "SalI" "SnaBI" "SpeI" "SphI" "SwaI" "XbaI" 
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COMMENT            6529 0 7837 0 7849 0 8233 0 0 "") 
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COMMENT        #2=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 3774 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 3706 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1006 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "PstI" "ctgcag" 6 0 6 3791 0 7333 0 7381 0 7649 
COMMENT            0 7936 0 8328 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "XmaI" "cccggg" 2 0 4 1472 0 2397 0 3164 0 8386 
COMMENT            0 0 "") 
COMMENT        #8=(CRSite (CStringList) "AgeI" "accggt" 2 0 3 1686 0 3853 0 8280 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "ApaI" "gggccc" 6 0 3 198 0 6998 0 7736 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 2357 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AvrII" "cctagg" 2 0 1 3810 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 6 761 0 804 0 2256 0 3626 
COMMENT             0 3783 0 6591 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "BbsI" "gaagac" 9 13 6 1641 1 1916 0 2101 0 
COMMENT             2471 0 2798 0 3333 1 0 "") 
COMMENT        #14=(CRSite (CStringList) "BglII" "agatct" 2 0 1 3028 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 4 2707 1 3013 1 3382 1 
COMMENT             5426 1 0 "") 
COMMENT        #16=(CRSite (CStringList) "BspEI" "tccgga" 2 0 2 3827 0 6911 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "EagI" "cggccg" 2 0 3 345 0 3408 0 3794 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "HpaI" "gttaac" 4 0 1 3981 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "KasI" "ggcgcc" 2 0 6 757 0 800 0 2252 0 3622 
COMMENT             0 3779 0 6587 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 3860 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "MfeI" "caattg" 2 0 1 3968 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 3767 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "NarI" "ggcgcc" 3 0 6 758 0 801 0 2253 0 3623 
COMMENT             0 3780 0 6588 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "NdeI" "catatg" 3 0 2 3802 0 6536 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 997 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 3794 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 1 7198 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 3741 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 4 365 0 3546 0 3755 0 
COMMENT             7063 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 3034 0 3720 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "SnaBI" "tacgta" 4 0 1 3838 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "SpeI" "actagt" 2 0 3 1678 0 3697 0 3724 0 0 
COMMENT             "") 
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COMMENT             0 0 "") 
COMMENT        #36=(CRSite (CStringList) "SwaI" "atttaaat" 5 0 1 2211 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "XbaI" "tctaga" 2 0 3 1024 0 3043 0 3862 0 0 
COMMENT             "") 
COMMENT        #38=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 3 1345 0 3734 0 8545 0 0 
COMMENT             "")) 
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COMMENT        #41=(CFSignal (CObList) "mKeima" 4 0 0 1692 2354 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
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COMMENT             (CStringList) 1 1 1 1 "") 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 08/18/21 11:38AM" 1) 
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COMMENT                          "Last Modified: 08/18/21 11:38AM" 1) 
COMMENT                     #53=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8825 bp" 1) 
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FEATURES             Location/Qualifiers
     Eukaryotic      ter 6692..8822
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="Unknown feature type:Eukaryotic"
     CDS             5291..6151
                     /vntifkey="4"
                     /label=Amp\(R)
     promoter        6692..8822
                     /vntifkey="29"
                     /label=B-actin\promoter
     CDS             1692..2354
                     /vntifkey="4"
                     /label=mKeima
     CDS             1020..1682
                     /vntifkey="4"
                     /label=FKBPhomo\2x
     CDS             2364..3026
                     /vntifkey="4"
                     /label=FKBPhomo2x\variant
     CDS             3042..3695
                     /vntifkey="4"
                     /label=FKBP\domain
BASE COUNT     1944 a      2431 c      2541 g      1909 t 
ORIGIN
        1 cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc cgccccggct 
       61 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg 
      121 taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg aaagccttaa 
      181 agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg tgcgtgtgtg 
      241 tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc gctgcgggcg 
      301 cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg gggcggtgcc 
      361 ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg 
      421 ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca cccccctccc 
      481 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg tggcgcgggg 
      541 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct 
      601 cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc ggctgtcgag 
      661 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc 
      721 ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg 
      781 gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg 
      841 tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag ggggacggct 
      901 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggggttt 
      961 atatcttccc ttctctgttc ctccgcagcc cccaagctag cgctaagctt gccaccatgc 
     1021 gctctagagg cgtccaagtc gaaaccatta gtcccggcga tggcagaaca tttcctaaaa 
     1081 ggggacaaac atgtgtcgtc cattatacag gcatgttgga ggacggcaaa aaggtggaca 
     1141 gtagtagaga tcgcaataaa cctttcaaat tcatgttggg aaaacaagaa gtcattaggg 
     1201 gatgggagga gggcgtggct caaatgtccg tcggccaacg cgctaagctc accatcagcc 
     1261 ccgactacgc atacggcgct accggacatc ccggaattat tccccctcac gctaccttgg 
     1321 tgtttgacgt cgaactgttg aagctcgaga ctagaggagt gcaggtggag actatctccc 
     1381 caggagacgg gcgcaccttc cccaagcgcg gccagacctg cgtggagcac tacaccggga 
     1441 tgcttgaaga tggaaagaaa gttgattcct cccgggacag aaacaagccc tttaagttta 
     1501 tgctaggcaa gcaggaggtg atccgaggct gggaagaagg ggttgcccag atgagtgtgg 
     1561 gtcagagagc caaactgact atatctccag attgtgccta tggtgccact gggcacccag 
     1621 gcatcatccc accacatacc actctcgtct tcgatgtgga gcttctaaaa ctggaaacta 
     1681 gtgaaccggt agtgagtgtg atcgctaaac aaatgaccta caaggtttat atgtcaggca 
     1741 cggtcaatgg acactacttt gaggtcgaag gcgatggaaa aggaaagcct tacgagggag 
     1801 agcagacagt aaagctcact gtcaccaagg gtggacctct gccatttgct tgggatattt 
     1861 tatcaccaca gcttcagtac ggaagcatac cattcaccaa gtaccctgaa gacatccctg 
     1921 attatttcaa gcagtcattc cctgagggat atacatggga gaggagcatg aactttgaag 
     1981 atggtgcagt gtgtactgtc agcaatgatt ccagcatcca aggcaactgt ttcatctaca 
     2041 atgtcaaaat ctctggtgag aactttcctc ccaatggacc tgttatgcag aagaagacac 
     2101 agggctggga acccagcact gagcgtctct ttgcacgaga tggaatgctg ataggaaacg 
     2161 attatatggc tctgaagttg gaaggaggtg gtcactattt gtgtgaattt aaatctactt 
     2221 acaaggcaaa gaagcctgtg aggatgccag ggcgccacga gattgaccgc aaactggatg 
     2281 taaccagtca caacagggat tacacatctg ttgagcagtg tgaaatagcc attgcacgcc 
     2341 actctttgct cggtggcgcg cctcgctcac gcggcgttca ggtggagact ataagcccgg 
     2401 gggatggtcg cacttttcct aaacgaggcc agacgtgcgt agttcactat accggcatgc 
     2461 ttgaagacgg caagaaggtg gatagctcta gggaccggaa taagcccttc aagttcatgc 
     2521 ttggtaagca agaagtgatc cggggttggg aagagggggt tgcccaaatg tcagtcggac 
     2581 aacgggcgaa gttgactatt tctcctgatt acgcttatgg tgcgacggga catcctggca 
     2641 tcataccgcc tcacgccact cttgtctttg atgtggaact tttgaagctg gaaacgcgag 
     2701 gggttcaagt tgagaccatt tcaccgggcg atggcagaac attcccgaaa aggggacaaa 
     2761 cgtgtgtagt acactatacg ggcatgctcg aagacgggaa aaaggttgac tcaagtagag 
     2821 atcggaacaa gcctttcaag ttcatgttgg ggaaacaaga ggtaatacgc gggtgggagg 
     2881 agggagtcgc tcagatgtct gtaggccaaa gggcgaagct caccatatca ccagattacg 
     2941 cttacggggc gacgggccat cccggaataa tacctccgca cgcgacactt gtttttgatg 
     3001 ttgaattgct taagttggag accagcagat ctgtcgaccg ctctagagga gtgcaggtgg 
     3061 aaaccatctc cccaggagac gggcgcacct tccccaagcg cggccagacc tgcgtggtgc 
     3121 actacaccgg gatgcttgaa gatggaaaga aatttgactc ctcccgggac agaaacaagc 
     3181 cctttaagtt tatgctaggc aagcaggagg tgatccgagg ctgggaagaa ggggttgccc 
     3241 agatgagtgt gggtcagaga gccaaactga ctatatctcc agattatgcc tatggtgcca 
     3301 ctgggcaccc aggcatcatc ccaccacatg ccactctcgt cttcgatgtg gagcttctaa 
     3361 aactggaaac tagaggcgtg caggtcgaga ccatcagccc cggcgacggc cgcacctttc 
     3421 ccaagagagg ccagacttgc gtggtccact acaccggcat gctggaggac ggcaagaagt 
     3481 tcgacagcag ccgcgaccgc aacaagccct tcaagttcat gctgggcaaa caggaagtga 
     3541 tccgcggctg ggaggaaggc gtggctcaga tgagcgtggg gcagcgggcc aagctgacca 
     3601 tcagccccga ctatgcctac ggcgccaccg gccaccccgg catcatcccc ccccacgcca 
     3661 ccctggtgtt cgacgtggag ctgctgaagc tggagactag ttaggaattc aggatccagt 
     3721 cgactagtac ttctcgagct ctgtacatgt ccgcggtcgc gacgtacgcg tatcgatggc 
     3781 gccagctgca ggcggccgcc atatgcatcc taggcctatt aatattccgg agtatacgta 
     3841 gccggctaac aaccggtacc tctagataag taatgatcat aatcagccat atcacatctg 
     3901 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa 
     3961 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca 
     4021 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 
     4081 ccaaactcat caatgtatct tatcatgtct ggatcaattc gtaatcatgg tcatagctgt 
     4141 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 
     4201 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 
     4261 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 
     4321 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 
     4381 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 
     4441 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 
     4501 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 
     4561 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 
     4621 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
     4681 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 
     4741 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
     4801 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
     4861 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 
     4921 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacagtaga aggacagtat 
     4981 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 
     5041 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 
     5101 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 
     5161 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 
     5221 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 
     5281 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 
     5341 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 
     5401 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 
     5461 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 
     5521 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 
     5581 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 
     5641 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 
     5701 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 
     5761 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 
     5821 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 
     5881 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 
     5941 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 
     6001 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 
     6061 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 
     6121 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 
     6181 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 
     6241 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta 
     6301 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt 
     6361 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 
     6421 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 
     6481 gtcggggttg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc 
     6541 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat 
     6601 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc 
     6661 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt 
     6721 cacgacgttg taaaacgacg gccagtgcca agttgggatc tttgcattgg cccacggctc 
     6781 tcaggatggg gatgctcccc ttcagcaccc ggttcccctt ggaaactgat ggtcctggct 
     6841 ctgtggcatg gcagtggcac tgtgaggagc ccctaccagc agcacacagt gggtttggca 
     6901 ctgccacgct ccggatgccg cgctctgatc caaccccata atcaagggaa cccgaattgc 
     6961 cccatcattg cccccaccac ccccatcctg ccgggccctc acaccccacg ctgccttgtg 
     7021 gtgacattcc ccagcccaaa cccacggctt catggctacc gcggggcatt tcccattgcc 
     7081 gccccattat cagctctgca cacctcccgc tgtacccatg cctcgtggct gcccttcttt 
     7141 gacgtataat cttctaatta atacccggcc ttgtcaaagt ggagcacaaa cgttaattaa 
     7201 ttccccagca ggcaggtaat taacagtgtg actccctttt tgctgcgagt ggggctgata 
     7261 cagagagatg tggcactatg gagcccacgg ggtcctggca ctgggtgccc acggaggtcc 
     7321 ccatgtgctg cagtgtcacc gcctccgagg tgacagtatt gtccctgcgg tgtccctgca 
     7381 gctcagctct gtccacaggg ccacctccag tttggagggg acacaatgca gccccgatgc 
     7441 aacccatcct cgcagcatcc cagggacaaa gaccccactg caagaccgca cacagggctg 
     7501 ggtcccgctc ccctaatatc tacagtgctt ttgcatggcc ccttaatcaa tgcagttaat 
     7561 cagcatgcgc tcatgcaccg ctctggagct gcaaagcccc tcgcagcgct gctcaccaac 
     7621 accgcgcacc gccccggccc agcctgcagc acgcgctgca aacaggaaag aaacaaaata 
     7681 ttgcccaaat gtaggcaaag gcattcggct gccttgacct ccgccgggcc gggccctgcc 
     7741 tgactcagct ccttactcag cgctcgcttc ctccctccgg ctgccaccgc cgcagcgcac 
     7801 accctgacaa agagtggccc ttaacgggct ctgaggtgca cccagcagtg cactcagcag 
     7861 tccaagggcc ggcctggagg tttgcaccgc tacgtgctga cattagcatt gaacttggcc 
     7921 ctgggtagtg ctgcaggccg ggcggggtgg gtgtagagag tgcagcgcgc gttgcacccg 
     7981 gtgccccttc ccctcccttg catcccagca ggctgcaccc cagcaccagg cccgtgcatg 
     8041 catgctcctg gtgttattgc agcctggtgc atgcatgcgt cttagtggtg cagcgctgtg 
     8101 catgcatcct ccttggtgtg tagcagctta gtgcatgcat acccctcggt gttattgctg 
     8161 ctctgtgcag gcacgctcat tgtatcactt catcccagtg catgcactca cactggagcg 
     8221 attgctgctc ggtgcacgca cactcattgt atcacgtcag ctcagtggct gcacgcacac 
     8281 cggtgttatt gctgctcggt gcgtgcatgc acatcagtgt cgctgcagct cagtgcatgc 
     8341 acgctcattg cccatcgcta tccctgcctc tcctgctggc gctccccggg aggtgacttc 
     8401 aaggggaccg caggaccacc tcgggggtgg ggggagggct gcacacgcgg accccgctcc 
     8461 ccctccccaa caaagcactg tggaatcaaa aaggggggag gggggatgga ggggcgcgtc 
     8521 acacccccgc cccacaccct cacctcgagg tgagccccac gttctgcttc actctcccca 
     8581 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag 
     8641 cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg 
     8701 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt 
     8761 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg 
     8821 ggagt 
//
