LOCUS       pLVX-IRES-hygro-       11672 bp    DNA     circular     18-AUG-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pLVX-IRES-hygro_(BEN-37).
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported maandag 14 mei 2018 from SnapGene Viewer 2.7.3
            http://www.snapgene.com
COMMENT     
            ApEinfo:methylated:1
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14004741|
COMMENT     VNTDBDATE|-14004741|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLVX-IRES-hygro-EGFP-DFCP1|
COMMENT     VNTAUTHORNAME|Wilco Nijenhuis|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 08/18/21 11:36AM" 1) 
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/.\">.</a>" 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/synthetic+DNA+construct\">synthetic DNA construct(synthetic DNA construct)</a>" 
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COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "CMV promoter human cytomegalovirus (CMV) immediate early promoter" 
COMMENT                                           1) 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #267=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2505..2708" 1) 
COMMENT                                     #268=(CLinePar 
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COMMENT                                     #269=(CLinePar 
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COMMENT                                           1) 
COMMENT                                     #272=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                   "CMV promoter") 
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COMMENT                                           "Start: 9198  End: 9228 (Complementary)" 
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COMMENT                                     #275=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "lac promoter promoter for the E. coli lac operon" 
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COMMENT                                     #279=(CLinePar 
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COMMENT                                           1) 
COMMENT                                     #282=(CLinePar 
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COMMENT                                   "lac promoter") 
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COMMENT                                           "Start: 11172  End: 11276 (Complementary)" 
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COMMENT                                     #285=(CLinePar 
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COMMENT                                           "AmpR promoter" 1) 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #287=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(11172..11276)" 1) 
COMMENT                                     #288=(CLinePar 
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COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /gene=\"bla\"" 1) 
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COMMENT                                     #292=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}" 
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COMMENT                                     #293=(CLinePar 
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COMMENT                           "Promoter Prokaryotic (3 total)") 
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COMMENT                                    (CObjectList 
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COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 9174  End: 9190" 1) 
COMMENT                                     #297=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "lac operator The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." 
COMMENT                                           1) 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #299=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   9174..9190" 1) 
COMMENT                                     #300=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                     #301=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /bound_moiety=\"lac repressor encoded by lacI\"" 
COMMENT                                           1) 
COMMENT                                     #302=(CLinePar 
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COMMENT                                           "   /ApEinfo_fwdcolor=pink" 1) 
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COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                     #304=(CLinePar 
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COMMENT                                           "   /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                           1) 
COMMENT                                     #305=(CLinePar 
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COMMENT                                           "   width 5 offset 0" 1))) 
COMMENT                                   "lac operator") 
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COMMENT                                    (CObjectList 
COMMENT                                     #307=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 9243  End: 9264" 1) 
COMMENT                                     #308=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "CAP binding site CAP binding activates transcription in the presence of cAMP." 
COMMENT                                           1) 
COMMENT                                     #309=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #310=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   9243..9264" 1) 
COMMENT                                     #311=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                     #312=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /bound_moiety=\"E. coli catabolite activator protein\"" 
COMMENT                                           1) 
COMMENT                                     #313=(CLinePar 
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COMMENT                                           "   /ApEinfo_fwdcolor=pink" 1) 
COMMENT                                     #314=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                     #315=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                           1) 
COMMENT                                     #316=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   width 5 offset 0" 1))) 
COMMENT                                   "CAP binding site"))) 
COMMENT                           "Protein Binding Site (2 total)") 
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COMMENT                            (CObjectList 
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COMMENT                                   (CGroupPar 
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COMMENT                                    (CObjectList 
COMMENT                                     #319=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 9552  End: 10140 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #320=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "ori high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" 
COMMENT                                           1) 
COMMENT                                     #321=(CLinePar 
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COMMENT                                     #322=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(9552..10140)" 1) 
COMMENT                                     #323=(CLinePar 
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COMMENT                                     #324=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /direction=LEFT" 1) 
COMMENT                                     #325=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_fwdcolor=pink" 1) 
COMMENT                                     #326=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_revcolor=pink" 1) 
COMMENT                                     #327=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                           1) 
COMMENT                                     #328=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   width 5 offset 0" 1))) "ori"))) 
COMMENT                           "Replication Origin (1 total)"))) "Feature Map") 
COMMENT              #329=(CFolderPar 
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COMMENT                     (CObjectList 
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COMMENT                             (CObjectList 
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COMMENT                                     (CObjectList 
COMMENT                                      #332=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Start: 1     End: 2808" 1) 
COMMENT                                      #333=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Original Location Description:" 1) 
COMMENT                                      #334=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   1..2808" 1) 
COMMENT                                      #335=(CLinePar 
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COMMENT                                            "Qualifiers:" 1) 
COMMENT                                      #336=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /organism=\"synthetic DNA construct\"" 
COMMENT                                            1) 
COMMENT                                      #337=(CLinePar 
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COMMENT                                            "   /mol_type=\"other DNA\"" 1) 
COMMENT                                      #338=(CLinePar 
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COMMENT                                            "   /ApEinfo_fwdcolor=pink" 1) 
COMMENT                                      #339=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /ApEinfo_revcolor=pink" 1) 
COMMENT                                      #340=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                            1) 
COMMENT                                      #341=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   width 5 offset 0" 1))) 
COMMENT                                    "source:synthetic DNA construct") 
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COMMENT                                     (CParagraph 3565 (0 0) 1 2 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #343=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Start: 3565  End: 3570" 1) 
COMMENT                                      #344=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Original Location Description:" 1) 
COMMENT                                      #345=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   3565..3570" 1) 
COMMENT                                      #346=(CLinePar 
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COMMENT                                      #347=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /organism=\"synthetic DNA construct\"" 
COMMENT                                            1) 
COMMENT                                      #348=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /mol_type=\"other DNA\"" 1) 
COMMENT                                      #349=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /ApEinfo_label=source:synthetic DNA construct" 
COMMENT                                            1) 
COMMENT                                      #350=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /ApEinfo_fwdcolor=pink" 1) 
COMMENT                                      #351=(CLinePar 
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COMMENT                                            "   /ApEinfo_revcolor=pink" 1) 
COMMENT                                      #352=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                            1) 
COMMENT                                      #353=(CLinePar 
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COMMENT                                            "   width 5 offset 0" 1))) 
COMMENT                                    "source:synthetic DNA construct(1)") 
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COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 5902 (0 0) 1 2 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #355=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Start: 5902  End: 11672" 1) 
COMMENT                                      #356=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Original Location Description:" 1) 
COMMENT                                      #357=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   5902..11672" 1) 
COMMENT                                      #358=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Qualifiers:" 1) 
COMMENT                                      #359=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /organism=\"synthetic DNA construct\"" 
COMMENT                                            1) 
COMMENT                                      #360=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /mol_type=\"other DNA\"" 1) 
COMMENT                                      #361=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /ApEinfo_label=source:synthetic DNA construct" 
COMMENT                                            1) 
COMMENT                                      #362=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /ApEinfo_fwdcolor=pink" 1) 
COMMENT                                      #363=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /ApEinfo_revcolor=pink" 1) 
COMMENT                                      #364=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                            1) 
COMMENT                                      #365=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   width 5 offset 0" 1))) 
COMMENT                                    "source:synthetic DNA construct(2)"))) 
COMMENT                            "Source (3 total)"))) 
COMMENT                    "Imported Features Not Shown on Map") 
COMMENT              #366=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #367=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93543936 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #368=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #8# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #369=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #8# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 4593 " 1))))) "AgeI: 1 site") 
COMMENT                            1 5 "ACCGGT" "TGGCCA") 
COMMENT                      #370=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93546560 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "ApaI: 3 sites") 5 1 "GGGCCC" 
COMMENT                            "CCCGGG") 
COMMENT                      #371=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 94322728 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "ApaLI: 7 sites") 1 5 "GTGCAC" 
COMMENT                            "CACGTG") 
COMMENT                      #372=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93547520 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "AscI: 0 sites") 2 6 "GGCGCGCC" 
COMMENT                            "CCGCGCGG") 
COMMENT                      #373=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93547872 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #374=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #11# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #375=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #11# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 6091 " 1))))) "AvrII: 1 site") 
COMMENT                            1 5 "CCTAGG" "GGATCC") 
COMMENT                      #376=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 94322312 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #377=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #378=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3536 " 1) 
COMMENT                                      #379=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 6529 " 1))))) "BamHI: 2 sites") 
COMMENT                            1 5 "GGATCC" "CCTAGG") 
COMMENT                      #380=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93549600 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #381=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #12# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #382=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #12# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 642 " 1))))) "BbeI: 1 site") 5 
COMMENT                            1 "GGCGCC" "CCGCGG") 
COMMENT                      #383=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93549920 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BbsI: 7 sites") 8 12 
COMMENT                            "GAAGACNNNNNNN" "CTTCTGNNNNNNN") 
COMMENT                      #384=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93553792 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BglII: 3 sites") 1 5 "AGATCT" 
COMMENT                            "TCTAGA") 
COMMENT                      #385=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93525216 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BsaI: 8 sites") 7 11 
COMMENT                            "GGTCTCNNNNNN" "CCAGAGNNNNNN") 
COMMENT                      #386=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93529536 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BspEI: 7 sites") 1 5 "TCCGGA" 
COMMENT                            "AGGCCT") 
COMMENT                      #387=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 94322344 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #388=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #389=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2181 " 1) 
COMMENT                                      #390=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 4269 " 1))))) "ClaI: 2 sites") 
COMMENT                            2 4 "ATCGAT" "TAGCTA") 
COMMENT                      #391=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93533856 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "EagI: 6 sites") 1 5 "CGGCCG" 
COMMENT                            "GCCGGC") 
COMMENT                      #392=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 94322376 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #393=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #394=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2804 " 1))))) "EcoRI: 1 site") 
COMMENT                            1 5 "GAATTC" "CTTAAG") 
COMMENT                      #395=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93534656 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "EcoRV: 6 sites") 3 3 "GATATC" 
COMMENT                            "CTATAG") 
COMMENT                      #396=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 94322152 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "HindIII: 8 sites") 1 5 "AAGCTT" 
COMMENT                            "TTCGAA") 
COMMENT                      #397=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93538176 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "HpaI: 0 sites") 3 3 "GTTAAC" 
COMMENT                            "CAATTG") 
COMMENT                      #398=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 46541672 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #399=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #20# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #400=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #20# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 638 " 1))))) "KasI: 1 site") 1 
COMMENT                            5 "GGCGCC" "CCGCGG") 
COMMENT                      #401=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 46542152 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #402=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #21# 0) 1 2 2 0 180) 
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COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Cla}I (4269)" "@N (@S)" 5 12.1851 
COMMENT                                   -17.4233 0 -10 4.19603 0.949103 #597#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #599=(CGroupWidget 
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COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Xma}I (4744)" "@N (@S)" 5 10.2454 
COMMENT                                   -22.6503 0 -10 4.72054 0.949103 #600#) 
COMMENT                             #602=(CScratch 
COMMENT                                   (CWidget 2 (1 #7# 2) 1 2 0 0 #458# 0 100) 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Xma}I (6525)" "@N (@S)" 5 -11.1846 
COMMENT                                   -8.71165 0 -10 4.74552 0.949103 #602#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #604=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #21# 0) 1 2 0 0 Nil -1479 100) 
COMMENT                            (CObjectList 
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COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Kpn}I (6385)" "@N (@S)" 5 -8.87792 
COMMENT                                   -10.454 0 -10 4.57068 0.949103 #605#) 
COMMENT                             #607=(CScratch 
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COMMENT                                   (LOGPEN 0 6 10053171) 8 1 1.80048 1.9 
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COMMENT                             #608=(CLabel (CWidget 0 (0 0) 1 2 0 0 #450# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Kpn}I (8323)" "@N (@S)" 5 -14.2738 
COMMENT                                   5.22699 0 -10 4.57068 0.949103 #607#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #609=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #25# 0) 1 2 0 0 Nil 1918975348 100) 
COMMENT                            (CObjectList 
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COMMENT                                   (LOGPEN 0 6 10053171) 8 1 4.99726 1.9 
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COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nde}I (2375)" "@N (@S)" 5 13.9426 
COMMENT                                   -3.48466 0 -10 4.39585 0.949103 #610#) 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nde}I (7000)" "@N (@S)" 5 -14.0204 
COMMENT                                   -3.48466 0 -10 4.52073 0.949103 #612#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #614=(CGroupWidget 
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COMMENT                                   1) 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Xba}I (5909)" "@N (@S)" 5 -3.9569 
COMMENT                                   -17.4233 0 -10 4.52073 0.949103 #615#) 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Xba}I (6535)" "@N (@S)" 5 -13.3177 
COMMENT                                   -5.22699 0 -10 4.47078 0.949103 #617#)) 
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FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="98"
     misc_feature    2818..3531
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=EGFP
     LTR             1..634
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="19"
                     /label=3'\LTR
                     /note="3' LTR 3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3571..5898
                     /ApEinfo_fwdcolor=#408080
                     /ApEinfo_revcolor=#408080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=DFCP1
     misc_feature    2815..2817
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=START
     misc_feature    681..806
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=HIV-1\Psi
                     /note="HIV-1 Psi packaging signal of human immunodeficiency virus type 1"
     misc_feature    1303..1536
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=RRE
                     /note="RRE The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm."
     misc_feature    2028..2143
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=cPPT/CTS
                     /note="cPPT/CTS central polypurine tract and central termination sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="9"
                     /label=CMV\enhancer
                     /note="CMV enhancer human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /ApEinfo_fwdcolor=#808080
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="30"
                     /label=CMV\promoter
                     /note="CMV promoter human cytomegalovirus (CMV) immediate early promoter"
     misc_feature    5939..6512
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=IRES
                     /note="IRES internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)"
     CDS             6555..7574
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="4"
                     /label=aminoglycoside\phosphotransferase\from\E.\coli
                     /note="HygR confers resistance to hygromycin"
     misc_feature    7594..8182
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=WPRE
                     /note="WPRE woodchuck hepatitis virus posttranscriptional regulatory element"
     CDS             complement(8065..8076)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="4"
                     /label=Factor\Xa\recognition\and\cleavage\site
                     /note="Factor Xa site"
     LTR             8389..9022
                     /ApEinfo_label=3' LTR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="19"
                     /label=3'\LTR(1)
                     /note="3' LTR 3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9150..9166)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="28"
                     /label=M13\rev
                     /note="M13 rev common sequencing primer, one of multiple similar variants"
     protein_bind    9174..9190
                     /bound_moiety="lac repressor encoded by lacI"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="31"
                     /label=lac\operator
                     /note="lac operator The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9198..9228)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="lac promoter promoter for the E. coli lac operon"
     protein_bind    9243..9264
                     /bound_moiety="E. coli catabolite activator protein"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="31"
                     /label=CAP\binding\site
                     /note="CAP binding site CAP binding activates transcription in the presence of cAMP."
     rep_origin      complement(9552..10140)
                     /direction=LEFT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="33"
                     /label=ori
                     /note="ori high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(10311..11171)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="4"
                     /label=beta-lactamase
                     /note="AmpR confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(11172..11276)
                     /gene="bla"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="30"
                     /label=bla
                     /note="AmpR promoter"
     polyA_signal    11324..11458
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="25"
                     /label=SV40\poly(A)\signal
                     /note="SV40 poly(A) signal SV40 polyadenylation signal"
     source          3565..3570
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /ApEinfo_label=source:synthetic DNA construct
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="98"
     source          5902..11672
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /ApEinfo_label=source:synthetic DNA construct
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="98"
BASE COUNT     2947 a      2930 c      3086 g      2709 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggtg 
     2821 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 
     2881 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 
     2941 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 
     3001 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac 
     3061 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 
     3121 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 
     3181 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 
     3241 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc 
     3301 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 
     3361 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 
     3421 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 
     3481 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggatcc 
     3541 ggcggaggga gcggaggggg ctcactcgag agtgcccaga cttccccagc agagaagggc 
     3601 ctgaatccgg ggctgatgtg ccaggaaagt tacgcttgca gcgggactga tgaagctatc 
     3661 tttgagtgtg atgagtgctg cagtctgcag tgtctccgct gcgaggagga gctccatcgg 
     3721 caggagcgcc tgagaaacca tgagcggata agactcaaac ctggccatgt cccttactgt 
     3781 gacctctgca agggtctcag tgggcattta ccaggtgtta ggcagagggc aatagtgagg 
     3841 tgccagacct gcaaaattaa cttgtgcctg gagtgccaga agaggactca ttctgggggt 
     3901 aacaaaagga gacaccctgt tactgtgtac aatgtcagta atctccagga gtcactggag 
     3961 gcagaagaga tggatgagga gaccaagagg aagaagatga ctgagaaggt tgtgagtttc 
     4021 ctcctagtag acgaaaatga agaaattcag gtaacaaatg aagaagactt tattagaaaa 
     4081 ttggactgca aacctgatca gcatctgaaa gtggtttcca tttttggaaa tactggtgat 
     4141 ggaaagtctc atactctcaa ccacactttc ttttatggtc gtgaagtctt taaaacctcc 
     4201 ccgacccagg agtcctgcac tgtgggagtg tgggcagcct atgacccagt tcacaaagta 
     4261 gcagtgatcg atacggaagg gctcctgggg gccaccgtga atctaagcca gagaacacgg 
     4321 ctgctgctta aggtcctggc catctcagac ctcgtcatct atcgaactca tgcagaccgg 
     4381 ctgcataacg acctcttcaa attccttggg gatgcctcag aagcttatct gaagcacttc 
     4441 accaaggagc tcaaggccac cactgctcgc tgtggcctgg atgtcccttt atccacactg 
     4501 ggccctgcag ttatcatctt ccatgagacc gtgcacaccc agctactggg ctctgatcat 
     4561 ccctcagagg tgccagagaa gctcatccag gaccggttcc ggaagctggg ccgtttccct 
     4621 gaagccttta gttccattca ctacaaggga acgaggactt acaaccctcc cacggacttt 
     4681 tctgggcttc ggcgtgcttt ggagcagcta ctagagaata acaccacccg ttctccccgg 
     4741 cacccgggag tcatcttcaa agccctgaag gcactaagtg accgcttcag cggtgagatc 
     4801 cccgatgacc agatggcgca cagctccttt tttccagatg agtatttcac ctgctcctcc 
     4861 ttgtgcctca gctgtggggt tggatgtaag aaaagcatga atcatgggaa ggaaggagtg 
     4921 cctcatgaag ccaagagccg ctgcagatac tcccaccagt atgacaaccg agtgtatacc 
     4981 tgcaaggcct gctatgagag aggcgaggaa gtcagtgtag tgcccaaaac atctgcttcc 
     5041 actgactccc cctggatggg tctcgcaaaa tatgcctggt ctgggtatgt gatcgaatgt 
     5101 cctaactgtg gcgtggtcta tcgtagtcgg cagtactggt ttggaaacca agatcctgtg 
     5161 gatacggtgg tgcggacaga gattgtgcat gtgtggcctg gaactgatgg gtttctgaag 
     5221 gacaacaaca atgctgccca gcgcctgttg gacgggatga acttcatggc tcagtcggtg 
     5281 tccgagctta gccttggacc caccaaggct gtgacttcct ggctgacaga ccagatcgcc 
     5341 cctgcctact ggaggcccaa ctcccagatt ctgagctgca acaagtgtgc gacgtccttt 
     5401 aaagataacg acactaagca tcactgccga gcctgtgggg agggcttctg tgacagctgt 
     5461 tcatcaaaga ctcggccagt gcctgagcgg ggctggggcc ctgcgccagt gcgggtctgt 
     5521 gacaactgct acgaagccag gaacgtccag ttagctgtta ccgaggcaca agtggacgat 
     5581 gaaggtggaa cgctcattgc tcggaaggtg ggcgaggccg tgcagaacac tctgggagcc 
     5641 gtggtgacag ccattgacat accactaggt ctggtaaagg acgcggccag gcctgcgtac 
     5701 tgggtgcctg accacgaaat cctccactgc cacaactgcc ggaaggagtt cagcatcaag 
     5761 ctctccaagc accactgccg ggcctgcgga cagggcttct gtgatgagtg ctcccatgac 
     5821 cgccgggctg ttccttctcg tggctgggac catcccgtcc gagtctgctt caactgcaat 
     5881 aaaaagcccg gtgaccttta aactagttct agagcggccg cggatctggc caattccgcc 
     5941 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg 
     6001 cgtttgtcta tatgtgattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga 
     6061 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa 
     6121 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa 
     6181 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct 
     6241 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg 
     6301 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg 
     6361 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca 
     6421 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg 
     6481 acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccggga tcctctagaa 
     6541 tggatagatc cggaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa 
     6601 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca 
     6661 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct 
     6721 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc 
     6781 ttgacattgg ggaatttagc gagagcctga cctattgcat ctcccgccgt gcacagggtg 
     6841 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg 
     6901 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac 
     6961 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc 
     7021 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc 
     7081 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg 
     7141 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga 
     7201 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt 
     7261 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag 
     7321 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct 
     7381 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc 
     7441 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga 
     7501 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga 
     7561 gggcaaagga ataggtcgac acgcgtctgg aacaatcaac ctctggatta caaaatttgt 
     7621 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 
     7681 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 
     7741 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 
     7801 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 
     7861 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 
     7921 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 
     7981 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc 
     8041 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 
     8101 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc 
     8161 tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg 
     8221 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac 
     8281 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt 
     8341 acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa 
     8401 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact 
     8461 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat 
     8521 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga 
     8581 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt 
     8641 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg 
     8701 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc 
     8761 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag 
     8821 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 
     8881 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 
     8941 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 
     9001 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 
     9061 cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg tttaccgtcg 
     9121 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 
     9181 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 
     9241 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 
     9301 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 
     9361 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 
     9421 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 
     9481 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 
     9541 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 
     9601 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 
     9661 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 
     9721 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 
     9781 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 
     9841 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 
     9901 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 
     9961 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 
    10021 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 
    10081 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 
    10141 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 
    10201 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 
    10261 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 
    10321 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 
    10381 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 
    10441 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 
    10501 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 
    10561 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 
    10621 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 
    10681 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 
    10741 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 
    10801 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 
    10861 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 
    10921 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 
    10981 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 
    11041 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 
    11101 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 
    11161 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 
    11221 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 
    11281 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc 
    11341 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 
    11401 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa 
    11461 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac 
    11521 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt 
    11581 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttttta aagaaattgt 
    11641 atttgttaaa tatgtactac aaacttagta gt 
//
