LOCUS       pLVX-IRES-hygro-       10247 bp    DNA     circular     18-AUG-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pLVX-IRES-hygro_(BEN-37).
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported maandag 14 mei 2018 from SnapGene Viewer 2.7.3
            http://www.snapgene.com
COMMENT     
            
            ApEinfo:methylated:1
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14004743|
COMMENT     VNTDBDATE|-14004743|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLVX-IRES-hygro-EGFP-STX17|
COMMENT     VNTAUTHORNAME|Wilco Nijenhuis|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #24=(CRSite (CStringList) "NarI" "ggcgcc" 3 0 1 639 0 0 "") 
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COMMENT                    "pLVX-IRES-hygro-EGFP-STX17" 2) 5 "" 0 4) 
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COMMENT                          "DNA 'pLVX-IRES-hygro-EGFP-STX17'" 1) 
COMMENT                     #66=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "synthetic circular DNA." 1) 
COMMENT                     #67=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
COMMENT                     #68=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 08/18/21 11:38AM" 1) 
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COMMENT                          "Last Modified: 08/18/21 11:38AM" 1) 
COMMENT                     #70=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 10247 bp" 1) 
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COMMENT                          "Keywords: pLVX-IRES-hygro_(BEN-37)" 1) 
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/.\">.</a>" 
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COMMENT                          "Version in the Original DB: ." 1) 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/synthetic+DNA+construct\">synthetic DNA construct(synthetic DNA construct)</a>" 
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COMMENT                                           "   /ApEinfo_fwdcolor=pink" 1) 
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COMMENT                                           "Start: 2505  End: 2708" 1) 
COMMENT                                     #275=(CLinePar 
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COMMENT                                           "CMV promoter human cytomegalovirus (CMV) immediate early promoter" 
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COMMENT                                           "lac promoter promoter for the E. coli lac operon" 
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COMMENT                                     #292=(CLinePar 
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COMMENT                                           "AmpR promoter" 1) 
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COMMENT                           "Promoter Prokaryotic (3 total)") 
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COMMENT                                     #307=(CLinePar 
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COMMENT                                           "lac operator The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." 
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COMMENT                                   "lac operator") 
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COMMENT                                     #318=(CLinePar 
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COMMENT                                           "CAP binding site CAP binding activates transcription in the presence of cAMP." 
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COMMENT                                           "   7818..7839" 1) 
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COMMENT                                           "   /bound_moiety=\"E. coli catabolite activator protein\"" 
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COMMENT                                           1) 
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COMMENT                                   "CAP binding site"))) 
COMMENT                           "Protein Binding Site (2 total)") 
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COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 8127  End: 8715 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #330=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "ori high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" 
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COMMENT                                     #338=(CLinePar 
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COMMENT                           "Replication Origin (1 total)"))) "Feature Map") 
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COMMENT                                            "Start: 1     End: 2808" 1) 
COMMENT                                      #343=(CLinePar 
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COMMENT                                      #350=(CLinePar 
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COMMENT                                            1) 
COMMENT                                      #351=(CLinePar 
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COMMENT                                    "Source_1") 
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COMMENT                                            "Start: 3565  End: 3570" 1) 
COMMENT                                      #354=(CLinePar 
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COMMENT                                            "Original Location Description:" 1) 
COMMENT                                      #355=(CLinePar 
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COMMENT                                            "   /mol_type=\"other DNA\"" 1) 
COMMENT                                      #359=(CLinePar 
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COMMENT                                            "   /ApEinfo_label=source:synthetic DNA construct" 
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COMMENT                                            1) 
COMMENT                                      #363=(CLinePar 
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COMMENT                                    "Source_2") 
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COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /organism=\"synthetic DNA construct\"" 
COMMENT                                            1) 
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COMMENT                                      #371=(CLinePar 
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COMMENT                                            "   /ApEinfo_label=source:synthetic DNA construct" 
COMMENT                                            1) 
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COMMENT                                            "   /ApEinfo_fwdcolor=pink" 1) 
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COMMENT                                      #375=(CLinePar 
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COMMENT                                            "   width 5 offset 0" 1))) 
COMMENT                                    "Source_3"))) "Source (3 total)"))) 
COMMENT                    "Imported Features Not Shown on Map") 
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COMMENT                     (CObjectList 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93541536 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "AgeI: 0 sites") 1 5 "ACCGGT" 
COMMENT                            "TGGCCA") 
COMMENT                      #378=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
COMMENT                                      #380=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #9# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 4632 " 1))))) "ApaI: 1 site") 
COMMENT                            5 1 "GGGCCC" "CCCGGG") 
COMMENT                      #381=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93528192 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "ApaLI: 7 sites") 1 5 "GTGCAC" 
COMMENT                            "CACGTG") 
COMMENT                      #382=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93541408 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "AscI: 0 sites") 2 6 "GGCGCGCC" 
COMMENT                            "CCGCGCGG") 
COMMENT                      #383=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
COMMENT                               #384=(CGroupPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #385=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #11# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 4666 " 1))))) "AvrII: 1 site") 
COMMENT                            1 5 "CCTAGG" "GGATCC") 
COMMENT                      #386=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93540800 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #387=(CGroupPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #388=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3536 " 1) 
COMMENT                                      #389=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 5104 " 1))))) "BamHI: 2 sites") 
COMMENT                            1 5 "GGATCC" "CCTAGG") 
COMMENT                      #390=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93540992 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
COMMENT                                      #392=(CLinePar 
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COMMENT                                            " N1: 642 " 1))))) "BbeI: 1 site") 5 
COMMENT                            1 "GGCGCC" "CCGCGG") 
COMMENT                      #393=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93540704 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BbsI: 6 sites") 8 12 
COMMENT                            "GAAGACNNNNNNN" "CTTCTGNNNNNNN") 
COMMENT                      #394=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93541088 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BglII: 4 sites") 1 5 "AGATCT" 
COMMENT                            "TCTAGA") 
COMMENT                      #395=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93541152 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BsaI: 4 sites") 7 11 
COMMENT                            "GGTCTCNNNNNN" "CCAGAGNNNNNN") 
COMMENT                      #396=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 93541888 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BspEI: 6 sites") 1 5 "TCCGGA" 
COMMENT                            "AGGCCT") 
COMMENT                      #397=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2181 " 1))))) "ClaI: 1 site") 
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COMMENT                              (CObjectList)) "EagI: 6 sites") 1 5 "CGGCCG" 
COMMENT                            "GCCGGC") 
COMMENT                      #401=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
COMMENT                               #402=(CGroupPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #403=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2804 " 1) 
COMMENT                                      #404=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 4147 " 1))))) "EcoRI: 2 sites") 
COMMENT                            1 5 "GAATTC" "CTTAAG") 
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COMMENT          #482=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
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FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="98"
     misc_feature    2818..3531
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=EGFP
     LTR             1..634
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="19"
                     /label=3'\LTR
                     /note="3' LTR 3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2815..2817
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=START
     misc_feature    681..806
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=HIV-1\Psi
                     /note="HIV-1 Psi packaging signal of human immunodeficiency virus type 1"
     misc_feature    4474..4476
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=STOP
     misc_feature    1303..1536
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=RRE
                     /note="RRE The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm."
     misc_feature    3571..4473
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=Stx17
     misc_feature    2028..2143
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=cPPT/CTS
                     /note="cPPT/CTS central polypurine tract and central termination sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="9"
                     /label=CMV\enhancer
                     /note="CMV enhancer human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /ApEinfo_fwdcolor=#808080
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="30"
                     /label=CMV\promoter
                     /note="CMV promoter human cytomegalovirus (CMV) immediate early promoter"
     misc_feature    4514..5087
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=IRES
                     /note="IRES internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)"
     CDS             5130..6149
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="4"
                     /label=aminoglycoside\phosphotransferase\from\E.\coli
                     /note="HygR confers resistance to hygromycin"
     misc_feature    6169..6757
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="21"
                     /label=WPRE
                     /note="WPRE woodchuck hepatitis virus posttranscriptional regulatory element"
     CDS             complement(6640..6651)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="4"
                     /label=Factor\Xa\recognition\and\cleavage\site
                     /note="Factor Xa site"
     LTR             6964..7597
                     /ApEinfo_label=3' LTR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="19"
                     /label=3'\LTR(1)
                     /note="3' LTR 3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7725..7741)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="28"
                     /label=M13\rev
                     /note="M13 rev common sequencing primer, one of multiple similar variants"
     protein_bind    7749..7765
                     /bound_moiety="lac repressor encoded by lacI"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="31"
                     /label=lac\operator
                     /note="lac operator The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7773..7803)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="lac promoter promoter for the E. coli lac operon"
     protein_bind    7818..7839
                     /bound_moiety="E. coli catabolite activator protein"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="31"
                     /label=CAP\binding\site
                     /note="CAP binding site CAP binding activates transcription in the presence of cAMP."
     rep_origin      complement(8127..8715)
                     /direction=LEFT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="33"
                     /label=ori
                     /note="ori high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(8886..9746)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="4"
                     /label=beta-lactamase
                     /note="AmpR confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(9747..9851)
                     /gene="bla"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="30"
                     /label=bla
                     /note="AmpR promoter"
     polyA_signal    9899..10033
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="25"
                     /label=SV40\poly(A)\signal
                     /note="SV40 poly(A) signal SV40 polyadenylation signal"
     source          3565..3570
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /ApEinfo_label=source:synthetic DNA construct
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="98"
     source          4477..10247
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /ApEinfo_label=source:synthetic DNA construct
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /vntifkey="98"
BASE COUNT     2686 a      2498 c      2651 g      2412 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggtg 
     2821 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 
     2881 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 
     2941 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 
     3001 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac 
     3061 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 
     3121 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 
     3181 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 
     3241 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc 
     3301 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 
     3361 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 
     3421 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 
     3481 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggatcc 
     3541 ggcggaggga gcggaggggg ctcactcgag tctgaagatg aagaaaaagt gaaattacgc 
     3601 cgtcttgaac cagctatcca gaaattcatt aagatagtaa tcccaacaga cctggaaagg 
     3661 ttaagaaagc accagataaa tattgagaag tatcaaaggt gcagaatctg ggacaagttg 
     3721 catgaagagc atatcaatgc aggacgtaca gttcagcaac tccgatccaa tatccgagaa 
     3781 attgagaaac tttgtttgaa agtccgaaag gatgacctag tacttctgaa gagaatgata 
     3841 gatcctgtta aagaagaagc atcagcagca acagcagaat ttctccaact ccatttggaa 
     3901 tctgtagaag aacttaagaa gcaatttaat gatgaagaaa ctttgctaca gcctcctttg 
     3961 accagatcca tgactgttgg tggagcattt catactactg aagctgaagc tagttctcag 
     4021 agtttgactc agatatatgc cttacctgaa attcctcaag atcaaaatgc tgcagaatcg 
     4081 tgggaaacct tagaagcgga cttaattgaa cttagccaac tggtcactga cttctctctc 
     4141 ctagtgaatt ctcagcagga gaagattgac agcattgcag accatgtcaa cagtgctgct 
     4201 gtgaatgttg aagagggaac caaaaactta gggaaggctg caaaatacaa gctggcagct 
     4261 ctgcctgtgg caggtgcact catcggggga atggtagggg gtcctattgg cctccttgca 
     4321 ggcttcaaag tggcaggaat tgcagctgca cttggtggtg gggtgttggg cttcacaggt 
     4381 ggaaaattga tacaaagaaa gaaacagaaa atgatggaga agctcacttc cagctgtcca 
     4441 gatcttccca gccaaactga caagaaatgc agttaaacta gttctagagc ggccgcggat 
     4501 ctggccaatt ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg 
     4561 aataaggccg gtgtgcgttt gtctatatgt gattttccac catattgccg tcttttggca 
     4621 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc 
     4681 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag 
     4741 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg 
     4801 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac 
     4861 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa 
     4921 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc 
     4981 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc 
     5041 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc 
     5101 cgggatcctc tagaatggat agatccggaa agcctgaact caccgcgacg tctgtcgaga 
     5161 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag 
     5221 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct 
     5281 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc 
     5341 cgattccgga agtgcttgac attggggaat ttagcgagag cctgacctat tgcatctccc 
     5401 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc 
     5461 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt 
     5521 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg 
     5581 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt 
     5641 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc 
     5701 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag 
     5761 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct 
     5821 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc 
     5881 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc 
     5941 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat 
     6001 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa 
     6061 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc 
     6121 ccagcactcg tccgagggca aaggaatagg tcgacacgcg tctggaacaa tcaacctctg 
     6181 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 
     6241 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 
     6301 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 
     6361 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 
     6421 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 
     6481 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 
     6541 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 
     6601 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 
     6661 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct 
     6721 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag 
     6781 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg 
     6841 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt 
     6901 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg 
     6961 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc 
     7021 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc 
     7081 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg 
     7141 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc 
     7201 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc 
     7261 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt 
     7321 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca 
     7381 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc 
     7441 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 
     7501 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 
     7561 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt 
     7621 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc 
     7681 tagcgtttac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct 
     7741 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 
     7801 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 
     7861 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 
     7921 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 
     7981 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 
     8041 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 
     8101 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 
     8161 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 
     8221 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 
     8281 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 
     8341 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 
     8401 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 
     8461 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 
     8521 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 
     8581 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 
     8641 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 
     8701 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 
     8761 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 
     8821 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 
     8881 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 
     8941 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 
     9001 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 
     9061 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 
     9121 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 
     9181 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 
     9241 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 
     9301 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 
     9361 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 
     9421 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 
     9481 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 
     9541 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 
     9601 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 
     9661 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 
     9721 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 
     9781 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 
     9841 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa 
     9901 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 
     9961 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 
    10021 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc 
    10081 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc 
    10141 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt 
    10201 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt 
//
