LOCUS       AY738222                4242 bp    DNA     circular SYN 08-NOV-2004
DEFINITION  Firefly luciferase reporter vector pGL4.10[luc2], complete
            sequence.
ACCESSION   AY738222
VERSION     AY738222.1  GI:55535618
KEYWORDS    .
SOURCE      Firefly luciferase reporter vector pGL4.10[luc2]
  ORGANISM  Firefly luciferase reporter vector pGL4.10[luc2]
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Almond,B. and Chauvin,F.
  TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
            Corporation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Almond,B. and Chauvin,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2004) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES             Location/Qualifiers
     misc_feature    3400..3621
                     /note="SV40 late poly(A) region"
     primer_bind     3693..3712
                     /note="reporter vector primer 4 (RVPrimer4)"
     rep_origin      3950..3950
                     /note="ColE1-derived"
     gene            complement(4741..5601)
                     /dnas_title="ampR"
                     /gene="ampR"
     CDS             complement(4741..5601)
                     /dnas_title="ampR"
                     /gene="ampR"
                     /note="AmpR"
                     /codon_start=1
                     /transl_table=11
                     /product="synthetic beta-lactamase"
                     /protein_id="AAV52870.1"
                     /db_xref="GI:55535620"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     polyA_signal    5706..5859
                     /note="synthetic; transcriptional pause site"
     primer_bind     complement(5808..5827)
                     /note="reporter vector primer 3 (RVPrimer3)"
     misc_feature    34..517
                     /note="RSV promoter"
     misc_feature    1716..3365
                     /note="Luciferase"
     misc_feature    546..612
                     /note="Exon1"
     misc_feature    1111..1169
                     /note="PSEx"
     misc_feature    1138..1146
                     /note="KozacATG"
     misc_feature    1670..1704
                     /note="Exon2"
     misc_feature    613..1110
                     /note=""
     misc_feature    1170..1669
                     /note=""
     source          1..5859
                     /dnas_title="ADDGENE pGL4_10_RSV_SF3B3Xon_Luciferase"
ORIGIN      
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagCC TGCAGGcgac aattgcatga
       61 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
      121 gtatctgagg ggactagggt gtgtttaggc gaaaagcggg gcttcggttg tacgcggtta
      181 ggagtcccct caggatatag tagtttcgct tttgcatagg gagggggaaa tgtagtctta
      241 tgcaatacac ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
      301 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
      361 aggaaggcaa cagacaggtc tgacatggat tggacgaacc actgaattgc gcattgcaga
      421 gataattgta tttaagtgcc tagctcgata caataaacgc catttgacca ttcaccacat
      481 tggtgtgcac ctccaaggcc acagatccGT TTAAACaagc ttggcaatcc ggtactgttg
      541 gtaaaTTTCT GTACAACTTA ACCTTGCAGA GAGCCACTGG CATCAGCTTT GCCATTCtTG
      601 GAAACTTTTC TGGTAAGTTC TCTCGTTACC ATCTTTTGAA ATTTTAAGTG AATTAATACA
      661 TATCTTGCTT AGTCTCTTGT GCAGGAAATG TTTTCCATTT ATGACAAAAC AGTTGTTGGT
      721 AAATGTAAAC AATTCAAATT ATGAGATGAG GTGTTAAGTT CTGGTGCATT ATCTGTTACC
      781 TATTTCAGAT GCATTTCCTA GTTCACAAAT TGTGTAATGA TTCTTGTCAG GGCACACTTT
      841 TCTTGGCTGC TTACCTAGTG TGGTTTGGTG CTTGAAGAGA TAGAACTTTT GTATGAGAGA
      901 AAAGGGAAAA TGCAGTTTAG GTAGGGAGTG CTGTCTGCAT AGAAACAGAT AATTTGCTTA
      961 CGTTTACCAT GTGGGGAATA TTTTTGTAAA GATGGATTAA GGCTAGGTTT GAATTGTGTG
     1021 AAATTTCAAA TATTGGATTA GGAAATACAA AGTTACTGAA AGTGAGGTAC TAATGTTTAT
     1081 AAAATAAAAA CTTTTTCTTG CCATTTGCAG ATTTAACATT TTTGAGTCAA TCCAAGTgcc
     1141 AccATGCAGG AGGTTCATGA TTGTGTAGAG TAAGACATAA TTTTGTTGAG GTTTAACTCT
     1201 GAATACTTAA TGTGGTACTG AATACTTAAT GTGGTACTGA GAGGCAGCCT AACTGACCAC
     1261 ACAGCATTCA TATTTCATGT TGTTATTTTC TCTGATCCTC ATTATGGCTC TTCCTGCGGG
     1321 TTTGAGTCTT CATTTGGGTA ATTTAGTTAT TCTTTGCTGC ATTTGATTAA GGAAATACGT
     1381 ATATGGGAAT TTGGAGATTT GTGACTGACT TCACTATGTC TAATTTTTTT TTGAGACGGA
     1441 GTTTTGCTCT GTTGCCCAGG CCGGAGTGCA GTGGCACAGT CTTGGCTCAC AGCAACCTCT
     1501 ACCTCCTGGG TTCAAGCGAT TCTCCTGCCT TAGCCTCCCG AGTAGCTGGG ATTACAGGCA
     1561 TGTGCCACCA CGCCTGGCTA ATTTTTGTAT TTTTAGTAGA GACGGGGTTT CACCATGGTG
     1621 GCCAGGCTGG GTTCTGGTTG TTTATGATCT TTATTTTTTG GTGATCTAGG AACCAAACAA
     1681 CAAGAAATTG TTGTTTCCCG TGGGTAAGAG gatccgaaga tgccaaaaac attaagaagg
     1741 gcccagcgcc attctaccca ctcgaagacg ggaccgccgg cgagcagctg cacaaagcca
     1801 tgaagcgcta cgccctggtg cccggcacca tcgcctttac cgacgcacat atcgaggtgg
     1861 acattaccta cgccgagtac ttcgagatga gcgttcggct ggcagaagct atgaagcgct
     1921 atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga gaatagcttg cagttcttca
     1981 tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc cccagctaac gacatctaca
     2041 acgagcgcga gctgctgaac agcatgggca tcagccagcc caccgtcgta ttcgtgagca
     2101 agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct accgatcata caaaagatca
     2161 tcatcatgga tagcaagacc gactaccagg gcttccaaag catgtacacc ttcgtgactt
     2221 cccatttgcc acccggcttc aacgagtacg acttcgtgcc cgagagcttc gaccgggaca
     2281 aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg attgcccaag ggcgtagccc
     2341 taccgcaccg caccgcttgt gtccgattca gtcatgcccg cgaccccatc ttcggcaacc
     2401 agatcatccc cgacaccgct atcctcagcg tggtgccatt tcaccacggc ttcggcatgt
     2461 tcaccacgct gggctacttg atctgcggct ttcgggtcgt gctcatgtac cgcttcgagg
     2521 aggagctatt cttgcgcagc ttgcaagact ataagattca atctgccctg ctggtgccca
     2581 cactatttag cttcttcgct aagagcactc tcatcgacaa gtacgaccta agcaacttgc
     2641 acgagatcgc cagcggcggg gcgccgctca gcaaggaggt aggtgaggcc gtggccaaac
     2701 gcttccacct accaggcatc cgccagggct acggcctgac agaaacaacc agcgccattc
     2761 tgatcacccc cgaaggggac gacaagcctg gcgcagtagg caaggtggtg cccttcttcg
     2821 aggctaaggt ggtggacttg gacaccggta agacactggg tgtgaaccag cgcggcgagc
     2881 tgtgcgtccg tggccccatg atcatgagcg gctacgttaa caaccccgag gctacaaacg
     2941 ctctcatcga caaggacggc tggctgcaca gcggcgacat cgcctactgg gacgaggacg
     3001 agcacttctt catcgtggac cggctgaaga gcctgatcaa atacaagggc taccaggtag
     3061 ccccagccga actggagagc atcctgctgc aacaccccaa catcttcgac gccggggtcg
     3121 ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc agtcgtcgtg ctggaacacg
     3181 gtaaaaccat gaccgagaag gagatcgtgg actatgtggc cagccaggtt acaaccgcca
     3241 agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc taaaggactg accggcaagt
     3301 tggacgcccg caagatccgc gagattctca ttaaggccaa gaagggcggc aagatcgccg
     3361 tgtaataatt ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg
     3421 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     3481 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     3541 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     3601 aaaacctcta caaatgtggt aaaatcgata aggatcgatc cgtcgaccga tgcccttgag
     3661 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
     3721 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt
     3781 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     3841 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     3901 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3961 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4021 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4081 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4141 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4201 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4261 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     4321 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     4381 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     4441 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     4501 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     4561 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     4621 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     4681 aaagtatata tgagtaaact tggtctgaca gcggccgcaa atgctaaacc actgcagtgg
     4741 ttaccagtgc ttgatcagtg aggcaccgat ctcagcgatc tgcctatttc gttcgtccat
     4801 agtggcctga ctccccgtcg tgtagatcac tacgattcgt gagggcttac catcaggccc
     4861 cagcgcagca atgatgccgc gagagccgcg ttcaccggcc cccgatttgt cagcaatgaa
     4921 ccagccagca gggagggccg agcgaagaag tggtcctgct actttgtccg cctccatcca
     4981 gtctatgagc tgctgtcgtg atgctagagt aagaagttcg ccagtgagta gtttccgaag
     5041 agttgtggcc attgctactg gcatcgtggt atcacgctcg tcgttcggta tggcttcgtt
     5101 caactctggt tcccagcggt caagccgggt cacatgatca cccatattat gaagaaatgc
     5161 agtcagctcc ttagggcctc cgatcgttgt cagaagtaag ttggccgcgg tgttgtcgct
     5221 catggtaatg gcagcactac acaattctct taccgtcatg ccatccgtaa gatgcttttc
     5281 cgtgaccggc gagtactcaa ccaagtcgtt ttgtgagtag tgtatacggc gaccaagctg
     5341 ctcttgcccg gcgtctatac gggacaacac cgcgccacat agcagtactt tgaaagtgct
     5401 catcatcggg aatcgttctt cggggcggaa agactcaagg atcttgccgc tattgagatc
     5461 cagttcgata tagcccactc ttgcacccag ttgatcttca gcatctttta ctttcaccag
     5521 cgtttcgggg tgtgcaaaaa caggcaagca aaatgccgca aagaagggaa tgagtgcgac
     5581 acgaaaatgt tggatgctca tactcgtcct ttttcaatat tattgaagca tttatcaggg
     5641 ttactagtac gtctctcaag gataagtaag taatattaag gtacgggagg tattggacag
     5701 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga
     5761 tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg
     5821 ctgtccccag tgcaagtgca ggtgccagaa catttctct
//

