LOCUS       AY738222                4242 bp    DNA     circular SYN 08-NOV-2004
DEFINITION  Firefly luciferase reporter vector pGL4.10[luc2], complete
            sequence.
ACCESSION   AY738222
VERSION     AY738222.1  GI:55535618
KEYWORDS    .
SOURCE      Firefly luciferase reporter vector pGL4.10[luc2]
  ORGANISM  Firefly luciferase reporter vector pGL4.10[luc2]
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Almond,B. and Chauvin,F.
  TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
            Corporation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Almond,B. and Chauvin,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2004) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES             Location/Qualifiers
     misc_feature    2802..3023
                     /note="SV40 late poly(A) region"
     primer_bind     3095..3114
                     /note="reporter vector primer 4 (RVPrimer4)"
     rep_origin      3352..3352
                     /note="ColE1-derived"
     gene            complement(4143..5003)
                     /dnas_title="ampR"
                     /gene="ampR"
     CDS             complement(4143..5003)
                     /dnas_title="ampR"
                     /gene="ampR"
                     /note="AmpR"
                     /codon_start=1
                     /transl_table=11
                     /product="synthetic beta-lactamase"
                     /protein_id="AAV52870.1"
                     /db_xref="GI:55535620"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     polyA_signal    5108..5261
                     /note="synthetic; transcriptional pause site"
     primer_bind     complement(5210..5229)
                     /note="reporter vector primer 3 (RVPrimer3)"
     misc_feature    34..517
                     /note="RSV promoter"
     misc_feature    1118..2767
                     /note="Luciferase"
     misc_feature    546..612
                     /note="Exon1"
     misc_feature    813..871
                     /note="PSEx"
     misc_feature    840..848
                     /note="KozacATG"
     misc_feature    1072..1106
                     /note="Exon2"
     misc_feature    613..812
                     /note=""
     misc_feature    872..1071
                     /note=""
     source          1..5261
                     /dnas_title="ADDGENE pGL4_10_RSV_SF3B3miniXon_Luciferase"
ORIGIN      
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagCC TGCAGGcgac aattgcatga
       61 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
      121 gtatctgagg ggactagggt gtgtttaggc gaaaagcggg gcttcggttg tacgcggtta
      181 ggagtcccct caggatatag tagtttcgct tttgcatagg gagggggaaa tgtagtctta
      241 tgcaatacac ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
      301 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
      361 aggaaggcaa cagacaggtc tgacatggat tggacgaacc actgaattgc gcattgcaga
      421 gataattgta tttaagtgcc tagctcgata caataaacgc catttgacca ttcaccacat
      481 tggtgtgcac ctccaaggcc acagatccGT TTAAACaagc ttggcaatcc ggtactgttg
      541 gtaaaTTTCT GTACAACTTA ACCTTGCAGA GAGCCACTGG CATCAGCTTT GCCATTCtTG
      601 GAAACTTTTC TGGTAAGTTC TCTCGTTACC ATCTTTTGAA ATTTTAAGTG AATTAATACA
      661 TATCTTGCTT AGTCTCTTGT GCAGGAAATG TTTTCCATTT ATGACAAAAC AGGAATTGTG
      721 TGAAATTTCA AATATTGGAT TAGGAAATAC AAAGTTACTG AAAGTGAGGT ACTAATGTTT
      781 ATAAAATAAA AACTTTTTCT TGCCATTTGC AGATTTAACA TTTTTGAGTC AATCCAAGTg
      841 ccAccATGCA GGAGGTTCAT GATTGTGTAG AGTAAGACAT AATTTTGTTG AGGTTTAACT
      901 CTGAATACTT AATGTGGTAC TGAATACTTA ATGTGGTACT GAGAGGCAGC CTAACTGACC
      961 ACACAGCATT CACGCCTGGC TAATTTTTGT ATTTTTAGTA GAGACGGGGT TTCACCATGG
     1021 TGGCCAGGCT GGGTTCTGGT TGTTTATGAT CTTTATTTTT TGGTGATCTA GGAACCAAAC
     1081 AACAAGAAAT TGTTGTTTCC CGTGGGTAAG AGgatccgaa gatgccaaaa acattaagaa
     1141 gggcccagcg ccattctacc cactcgaaga cgggaccgcc ggcgagcagc tgcacaaagc
     1201 catgaagcgc tacgccctgg tgcccggcac catcgccttt accgacgcac atatcgaggt
     1261 ggacattacc tacgccgagt acttcgagat gagcgttcgg ctggcagaag ctatgaagcg
     1321 ctatgggctg aatacaaacc atcggatcgt ggtgtgcagc gagaatagct tgcagttctt
     1381 catgcccgtg ttgggtgccc tgttcatcgg tgtggctgtg gccccagcta acgacatcta
     1441 caacgagcgc gagctgctga acagcatggg catcagccag cccaccgtcg tattcgtgag
     1501 caagaaaggg ctgcaaaaga tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat
     1561 catcatcatg gatagcaaga ccgactacca gggcttccaa agcatgtaca ccttcgtgac
     1621 ttcccatttg ccacccggct tcaacgagta cgacttcgtg cccgagagct tcgaccggga
     1681 caaaaccatc gccctgatca tgaacagtag tggcagtacc ggattgccca agggcgtagc
     1741 cctaccgcac cgcaccgctt gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa
     1801 ccagatcatc cccgacaccg ctatcctcag cgtggtgcca tttcaccacg gcttcggcat
     1861 gttcaccacg ctgggctact tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga
     1921 ggaggagcta ttcttgcgca gcttgcaaga ctataagatt caatctgccc tgctggtgcc
     1981 cacactattt agcttcttcg ctaagagcac tctcatcgac aagtacgacc taagcaactt
     2041 gcacgagatc gccagcggcg gggcgccgct cagcaaggag gtaggtgagg ccgtggccaa
     2101 acgcttccac ctaccaggca tccgccaggg ctacggcctg acagaaacaa ccagcgccat
     2161 tctgatcacc cccgaagggg acgacaagcc tggcgcagta ggcaaggtgg tgcccttctt
     2221 cgaggctaag gtggtggact tggacaccgg taagacactg ggtgtgaacc agcgcggcga
     2281 gctgtgcgtc cgtggcccca tgatcatgag cggctacgtt aacaaccccg aggctacaaa
     2341 cgctctcatc gacaaggacg gctggctgca cagcggcgac atcgcctact gggacgagga
     2401 cgagcacttc ttcatcgtgg accggctgaa gagcctgatc aaatacaagg gctaccaggt
     2461 agccccagcc gaactggaga gcatcctgct gcaacacccc aacatcttcg acgccggggt
     2521 cgccggcctg cccgacgacg atgccggcga gctgcccgcc gcagtcgtcg tgctggaaca
     2581 cggtaaaacc atgaccgaga aggagatcgt ggactatgtg gccagccagg ttacaaccgc
     2641 caagaagctg cgcggtggtg ttgtgttcgt ggacgaggtg cctaaaggac tgaccggcaa
     2701 gttggacgcc cgcaagatcc gcgagattct cattaaggcc aagaagggcg gcaagatcgc
     2761 cgtgtaataa ttctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat
     2821 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     2881 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     2941 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     3001 gtaaaacctc tacaaatgtg gtaaaatcga taaggatcga tccgtcgacc gatgcccttg
     3061 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     3121 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc
     3181 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3241 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3301 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3361 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3421 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3481 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3541 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3601 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3661 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3721 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3781 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3841 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3901 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3961 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4021 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     4081 ctaaagtata tatgagtaaa cttggtctga cagcggccgc aaatgctaaa ccactgcagt
     4141 ggttaccagt gcttgatcag tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc
     4201 atagtggcct gactccccgt cgtgtagatc actacgattc gtgagggctt accatcaggc
     4261 cccagcgcag caatgatgcc gcgagagccg cgttcaccgg cccccgattt gtcagcaatg
     4321 aaccagccag cagggagggc cgagcgaaga agtggtcctg ctactttgtc cgcctccatc
     4381 cagtctatga gctgctgtcg tgatgctaga gtaagaagtt cgccagtgag tagtttccga
     4441 agagttgtgg ccattgctac tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg
     4501 ttcaactctg gttcccagcg gtcaagccgg gtcacatgat cacccatatt atgaagaaat
     4561 gcagtcagct ccttagggcc tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg
     4621 ctcatggtaa tggcagcact acacaattct cttaccgtca tgccatccgt aagatgcttt
     4681 tccgtgaccg gcgagtactc aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc
     4741 tgctcttgcc cggcgtctat acgggacaac accgcgccac atagcagtac tttgaaagtg
     4801 ctcatcatcg ggaatcgttc ttcggggcgg aaagactcaa ggatcttgcc gctattgaga
     4861 tccagttcga tatagcccac tcttgcaccc agttgatctt cagcatcttt tactttcacc
     4921 agcgtttcgg ggtgtgcaaa aacaggcaag caaaatgccg caaagaaggg aatgagtgcg
     4981 acacgaaaat gttggatgct catactcgtc ctttttcaat attattgaag catttatcag
     5041 ggttactagt acgtctctca aggataagta agtaatatta aggtacggga ggtattggac
     5101 aggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
     5161 gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata
     5221 ggctgtcccc agtgcaagtg caggtgccag aacatttctc t
//

