LOCUS       Exported                3971 bp ds-DNA     circular SYN 05-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...1628192680460.542
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 5, 2021 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             165..956
                     /codon_start=1
                     /label=smR CDS
                     /note="smR"
                     /translation="LFADYLGDLAFHVVDKFFQLICARGQAIFFLSKISLSSFKYDGLI
                     LGRQALHCPVGSDILRRDFAGYCAVPNAGQRKHYISLIASPVGRRVP*R*GFI*RLK*I
                     LFRNRIKEFLRRWTYQGNAMFSCFCQQDSQINVDRGWLEDTCKNVIALPFSKLQFALSW
                     ITPRNDVVVHNNGDFYSAENLALSRGSRSFQKVVDQSSPRCFIKPYGHRNQQINITVWL
                     QAAIHCGAVQMYGQQRRFEMALDDANYL**LSRYFGDHRFPH"
     misc_feature    complement(957..1048)
                     /label=ampR promoter (for smR)
                     /note="ampR promoter (for smR)"
     misc_feature    1096..1834
                     /label=CloDF13 origin
                     /note="CloDF13 origin; by benchling"
     CDS             2233..3162
                     /codon_start=1
                     /label=araC CDS
                     /note="araC"
                     /translation="L*QLDGYIIHFFFTTGTELARAGPGAFFKYPREIELIVKTNIATD
                     GGDRHPGGAQKQLRLADTLVLAPA*DANP*LLAEKM*QTRRRQANMLCDAGDIKIAVCQ
                     VIADVLTSLAYPIIHRWMERLVNRFHAPQ*QLLKQIYRQQLRIAPFPLPGVNDLPKQVA
                     EMRLVRFIRAKEPRIGKY*RPVKPFMPVGARTKVNPLVIPFASLRMTTVVMNLSWREQQ
                     NITRSANKFSSLIFHHPLTANGEIENITFHSQRSVDKKIEITVGLNRR*TRHQMGIKRV
                     SRQQGIILRFSHTFHTPAIQRRNQLSILH"
     misc_feature    3141..3156
                     /label=araO2
                     /note="araO2"
     misc_feature    3212..3235
                     /label=pBait F sequencing
                     /note="oKB330 F sequencing"
     misc_feature    3298..3309
                     /label=araO1
                     /note="araO1"
     misc_feature    3385..3390
                     /label=-35
                     /note="-35"
                     /note="Geneious type: promoter"
     promoter        3409..3414
                     /label=-10
     misc_feature    3427..3447
                     /label=MS2hairpin
                     /note="MS2 stem 2"
     misc_feature    3459..3542
                     /label=ChiX
     misc_feature    complement(3600..3620)
                     /label=pBait R sequencing
                     /note="Hongbaek R sequencing"
     misc_feature    3631..3788
                     /label=rrnB terminator
                     /note="rrnB terminator"
     misc_feature    3754..3797
                     /label=rrnB T1
                     /note="rrnB T1"
     misc_feature    3929..3956
                     /label=rrnB T2
                     /note="rrnB T2"
ORIGIN
        1 cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac
       61 cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcatcg
      121 tggccggatc ttgcggcccc tcggcttgaa cgaattgtta gacattattt gccgactacc
      181 ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc
      241 aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac
      301 tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg
      361 gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc
      421 cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt
      481 tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct
      541 cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct
      601 gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc
      661 cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc
      721 tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca
      781 tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca
      841 tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg
      901 acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatactc
      961 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1021 tttgaatgta tttagaaaaa taaacaaata gctagctcac tcggtcgcta cgctccgggc
     1081 gtgagactgc ggcgggcgct gcggacacat acaaagttac ccacagattc cgtggataag
     1141 caggggacta acatgtgagg caaaacagca gggccgcgcc ggtggcgttt ttccataggc
     1201 tccgccctcc tgccagagtt cacataaaca gacgcttttc cggtgcatct gtgggagccg
     1261 tgaggctcaa ccatgaatct gacagtacgg gcgaaacccg acaggactta aagatcccca
     1321 ccgtttccgg cgggtcgctc cctcttgcgc tctcctgttc cgaccctgcc gtttaccgga
     1381 tacctgttcc gcctttctcc cttacgggaa gtgtggcgct ttctcatagc tcacacactg
     1441 gtatctcggc tcggtgtagg tcgttcgctc caagctgggc tgtaagcaag aactccccgt
     1501 tcagcccgac tgctgcgcct tatccggtaa ctgttcactt gagtccaacc cggaaaagca
     1561 cggtaaaacg ccactggcag cagccattgg taactgggag ttcgcagagg atttgtttag
     1621 ctaaacacgc ggttgctctt gaagtgtgcg ccaaagtccg gctacactgg aaggacagat
     1681 ttggttgctg tgctctgcga aagccagtta ccacggttaa gcagttcccc aactgactta
     1741 accttcgatc aaaccacctc cccaggtggt tttttcgttt acagggcaaa agattacgcg
     1801 cagaaaaaaa ggatctcaag aagatccttt gatcttttct actgaaccgc tctagatttc
     1861 agtgcaattt atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgta
     1921 attctcatgt tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
     1981 aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg
     2041 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     2101 caacgcgcgg ggagaggcgg tttgcgtatt gggcgccatc gatgcataat gtgcctgtca
     2161 aatggacgaa gcagggattc tgcaaaccct atgctactcc gtcaagccgt caattgtctg
     2221 attcgttacc aattatgaca acttgacggc tacatcattc actttttctt cacaaccggc
     2281 acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg
     2341 atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag
     2401 cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg
     2461 ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc
     2521 gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg
     2581 attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg
     2641 ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat
     2701 gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc
     2761 cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa
     2821 gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc
     2881 tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt
     2941 caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg
     3001 gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg
     3061 ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat
     3121 actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt gccgtcactg
     3181 cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta aaagcattct
     3241 gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg
     3301 cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt
     3361 atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttgaat
     3421 tcagaaaaca tgaggatcac ccatgtctgc agcccgggac accgtcgctt aaagtgacgg
     3481 cataataata aaaaaatgaa attcctcttt gacgggccaa tagcgatatt ggccattttt
     3541 ttaagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag
     3601 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac
     3661 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc
     3721 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac
     3781 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     3841 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg
     3901 ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg
     3961 tttctacaaa c
//
