LOCUS       174663_pCH1_pCDF        3901 bp ds-DNA     circular SYN 20-JAN-2022
DEFINITION  .
ACCESSION   urn.local...1628191615913.521
VERSION
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 22, 2017 from SnapGene Viewer 3.3.4 to Vector
            NTI(R) format http://www.snapgene.com
FEATURES             Location/Qualifiers
     CDS             165..956
                     /codon_start=1
                     /note="smR"
                     /label="smR CDS"
     misc_feature    957..1048
                     /note="ampR promoter (for smR)"
                     /label="ampR promoter (for smR)"
     misc_feature    1096..1834
                     /note="CloDF13 origin; by benchling"
                     /label="CloDF13 origin"
     CDS             2233..3162
                     /codon_start=1
                     /note="araC"
                     /label="araC CDS"
     misc_feature    3141..3156
                     /note="araO2"
                     /label="araO2"
     misc_feature    3212..3235
                     /note="oKB330 F sequencing"
                     /label="pBait F sequencing"
     misc_feature    3298..3309
                     /note="araO1"
                     /label="araO1"
     misc_feature    3385..3390
                     /note="-35"
                     /note="Geneious type: promoter"
                     /label="-35"
     promoter        3409..3414
                     /label="-10"
     misc_feature    3427..3447
                     /note="MS2 stem 2"
                     /label="MS2hairpin"
     misc_feature    complement(3530..3550)
                     /note="Hongbaek R sequencing"
                     /label="pBait R sequencing "
     misc_feature    3561..3718
                     /note="rrnB terminator"
                     /label="rrnB terminator"
     misc_feature    3684..3727
                     /note="rrnB T1"
                     /label="rrnB T1"
     misc_feature    3859..3886
                     /note="rrnB T2"
                     /label="rrnB T2"
ORIGIN      
        1 cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac
       61 cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcatcg
      121 tggccggatc ttgcggcccc tcggcttgaa cgaattgtta gacattattt gccgactacc
      181 ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc
      241 aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac
      301 tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg
      361 gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc
      421 cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt
      481 tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct
      541 cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct
      601 gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc
      661 cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc
      721 tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca
      781 tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca
      841 tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg
      901 acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatactc
      961 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1021 tttgaatgta tttagaaaaa taaacaaata gctagctcac tcggtcgcta cgctccgggc
     1081 gtgagactgc ggcgggcgct gcggacacat acaaagttac ccacagattc cgtggataag
     1141 caggggacta acatgtgagg caaaacagca gggccgcgcc ggtggcgttt ttccataggc
     1201 tccgccctcc tgccagagtt cacataaaca gacgcttttc cggtgcatct gtgggagccg
     1261 tgaggctcaa ccatgaatct gacagtacgg gcgaaacccg acaggactta aagatcccca
     1321 ccgtttccgg cgggtcgctc cctcttgcgc tctcctgttc cgaccctgcc gtttaccgga
     1381 tacctgttcc gcctttctcc cttacgggaa gtgtggcgct ttctcatagc tcacacactg
     1441 gtatctcggc tcggtgtagg tcgttcgctc caagctgggc tgtaagcaag aactccccgt
     1501 tcagcccgac tgctgcgcct tatccggtaa ctgttcactt gagtccaacc cggaaaagca
     1561 cggtaaaacg ccactggcag cagccattgg taactgggag ttcgcagagg atttgtttag
     1621 ctaaacacgc ggttgctctt gaagtgtgcg ccaaagtccg gctacactgg aaggacagat
     1681 ttggttgctg tgctctgcga aagccagtta ccacggttaa gcagttcccc aactgactta
     1741 accttcgatc aaaccacctc cccaggtggt tttttcgttt acagggcaaa agattacgcg
     1801 cagaaaaaaa ggatctcaag aagatccttt gatcttttct actgaaccgc tctagatttc
     1861 agtgcaattt atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgta
     1921 attctcatgt tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
     1981 aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg
     2041 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     2101 caacgcgcgg ggagaggcgg tttgcgtatt gggcgccatc gatgcataat gtgcctgtca
     2161 aatggacgaa gcagggattc tgcaaaccct atgctactcc gtcaagccgt caattgtctg
     2221 attcgttacc aattatgaca acttgacggc tacatcattc actttttctt cacaaccggc
     2281 acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg
     2341 atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag
     2401 cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg
     2461 ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc
     2521 gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg
     2581 attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg
     2641 ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat
     2701 gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc
     2761 cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa
     2821 gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc
     2881 tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt
     2941 caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg
     3001 gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg
     3061 ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat
     3121 actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt gccgtcactg
     3181 cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta aaagcattct
     3241 gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg
     3301 cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt
     3361 atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttgaat
     3421 tcagaaaaca tgaggatcac ccatgtctgc agcccgggac ctgcaggcat gcaagcttgg
     3481 ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag
     3541 cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat
     3601 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag
     3661 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     3721 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
     3781 atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
     3841 ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
     3901 c
//
