LOCUS       EFS-Flag-NLS-evoA1-nCa 12604 bp ds-DNA     circular     03-AUG-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        17..243
                     /label="RSV promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     LTR             244..424
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    471..596
                     /label="HIV-1 Ψ"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1089..1322
                     /label="RRE"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1735..1852
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        1921..2132
                     /label="EF-1α core promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2130..2145
                     /label="overlap gibson"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2152..2161
                     /label="Kozak sequence"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             2161..2226
                     /label="3xFLAG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(2202..2226)
                     /label="vector_reverse_Lenti_BE4max"
                     /note="sequence: cttatcgtcatcgtctttgtaatca"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          2212..2246
                     /label="Insert_forward_Lenti_BE4max"
                     /note="sequence: gacgatgacgataagatgaaacggacagccgacgg"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2227..2283
                     /label="SV40NLS"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             2227..7734
                     /label="Translation 2227-7734"
     misc_feature    2287..2967
                     /label="BE4max"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2968..3063
                     /label="linker"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(2981..2998)
                     /label="XmaI for BE4max Reverse"
                     /note="sequence: tgccagaggAtcctccgc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          2999..3023
                     /label="XmaI for BE4max Forward"
                     /note="sequence: gCGAGACACCcgggACAAGCGAGTC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    3064..7164
                     /label="nCas9"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    7165..7734
                     /label="2X UGI"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             7735..7785
                     /label="Translation 7735-7785"
     primer          complement(7746..7779)
                     /label="insert_reverse_Lenti_BE4max"
                     /note="sequence: cctcttcttcttgggctcgaattcgctgccgtcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          7765..7786
                     /label="vector_forward_Lenti_BE4max"
                     /note="sequence: cccaagaagaagaggaaagtcg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             7786..7857
                     /label="Translation 7786-7857"
     CDS             7801..7857
                     /label="P2A"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             7858..8457
                     /label="PuroR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    8473..9061
                     /label="WPRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(8944..8955)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     LTR             9133..9366
                     /label="3' LTR (ΔU3)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     polyA_signal    9444..9565
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      9605..9740
                     /label="SV40 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        complement(9761..9779)
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(9789..9805)
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     rep_origin      9947..10402
                     /label="f1 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        10428..10532
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             10533..10601
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             10602..11393
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     rep_origin      11564..12152
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    12440..12461
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        12476..12481
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        12482..12499
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        12500..12506
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    12514..12530
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     primer_bind     12538..12554
                     /label="M13 rev"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     promoter        12575..12593
                     /label="T3 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
       61 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
      121 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
      181 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
      241 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      301 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      361 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      421 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      481 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      541 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      601 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
      661 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
      721 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
      781 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
      841 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
      901 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
      961 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1021 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1081 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1141 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1201 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1261 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1321 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1381 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1441 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1501 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1561 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1621 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     1681 tcaccattat cgtttcagac ccacctccca accccgaggg gacccagacc acaattttaa
     1741 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     1801 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     1861 ttacagggac agcagagatc cactttggcg ccggctcgag tggctccggt gcccgtcagt
     1921 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     1981 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2041 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2101 tttttcgcaa cgggtttgcc gccagaacac aggtgtcgtg acgcgggatc cgccaccatg
     2161 gactataagg accacgacgg agactacaag gatcatgata ttgattacaa agacgatgac
     2221 gataagatga aacggacagc cgacggaagc gagttcgagt caccaaagaa gaagcggaaa
     2281 gtcagttcaa agactgggcc tgtcgccgtc gatccaaccc tgcgccgccg gattgaacct
     2341 cacgagtttg aagtgttctt tgacccccgg gagctgagaa aggagacatg cctgctgtac
     2401 gagatcaact ggggaggcag gcactccatc tggaggcaca cctctcagaa cacaaataag
     2461 cacgtggagg tgaacttcat cgagaagttt accacagagc ggtacttctg ccccaatacc
     2521 agatgtagca tcacatggtt tctgagctgg tccccttgcg gagagtgtag cagggccatc
     2581 accgagttcc tgtccagata tccaaatgtg acactgttta tctacatcgc caggctgtat
     2641 cacctggcaa acccaaggaa taggcagggc ctgcgcgatc tgatcagctc cggcgtgacc
     2701 atccagatca tgacagagca ggagtccggc tactgctggc acaacttcgt gaattattct
     2761 cctagcaacg agtcccactg gcctaggtac ccacacctgt gggtgcgcct gtacgtgctg
     2821 gagctgtatt gcatcatcct gggcctgccc ccttgtctga atatcctgcg gagaaagcag
     2881 agccagctga cctcctttac aatcgccctg cagtcttgtc actatcagag gctgccaccc
     2941 cacatcctgt gggccacagg cctgaagtct ggcggatcta gcggaggatc ctctggcagc
     3001 gagacaccag gaacaagcga gtcagcaaca ccagagagca gtggcggcag cagcggcggc
     3061 agcgacaaga agtacagcat cggcctggcc atcggcacca actctgtggg ctgggccgtg
     3121 atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg
     3181 cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag
     3241 gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc
     3301 tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga
     3361 ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc
     3421 aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag
     3481 aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac
     3541 atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac
     3601 gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc
     3661 atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga
     3721 cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggaaac
     3781 ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag
     3841 gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc
     3901 cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc
     3961 ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct
     4021 atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg
     4081 cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc
     4141 ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg
     4201 gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg
     4261 aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac
     4321 gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc
     4381 gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc
     4441 agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa
     4501 gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag
     4561 aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg
     4621 tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg
     4681 agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc
     4741 gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc
     4801 tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt
     4861 atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg
     4921 ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc
     4981 cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc
     5041 aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg
     5101 gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac
     5161 agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg
     5221 cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca
     5281 gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg
     5341 atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga
     5401 atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc
     5461 gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg
     5521 gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga tgtggaccat
     5581 atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc
     5641 gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag
     5701 aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg
     5761 accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag
     5821 ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac
     5881 actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc
     5941 aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac
     6001 taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag
     6061 taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag
     6121 atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc
     6181 aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg
     6241 cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt
     6301 gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg
     6361 cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc
     6421 gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc
     6481 tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg
     6541 aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac
     6601 tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag
     6661 tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg
     6721 cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc
     6781 cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa
     6841 cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg
     6901 atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag
     6961 cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc
     7021 cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa
     7081 gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc
     7141 gacctgtctc agctgggagg tgacagcggc gggagcggcg ggagcggggg gagcactaat
     7201 ctgagcgaca tcattgagaa ggagactggg aaacagctgg tcattcagga gtccatcctg
     7261 atgctgcctg aggaggtgga ggaagtgatc ggcaacaagc cagagtctga catcctggtg
     7321 cacaccgcct acgacgagtc cacagatgag aatgtgatgc tgctgacctc tgacgccccc
     7381 gagtataagc cttgggccct ggtcatccag gattctaacg gcgagaataa gatcaagatg
     7441 ctgagcggag gatccggagg atctggaggc agcaccaacc tgtctgacat catcgagaag
     7501 gagacaggca agcagctggt catccaggag agcatcctga tgctgcccga agaagtcgaa
     7561 gaagtgatcg gaaacaagcc tgagagcgat atcctggtcc ataccgccta cgacgagagt
     7621 accgacgaaa atgtgatgct gctgacatcc gacgccccag agtataagcc ctgggctctg
     7681 gtcatccagg attccaacgg agagaacaaa atcaaaatgc tgtctggcgg ctcaaaaaga
     7741 accgccgacg gcagcgaatt cgagcccaag aagaagagga aagtcgctag cggcagcggc
     7801 gccaccaact tcagcctgct gaagcaggcc ggcgacgtgg aggagaaccc cggccccatg
     7861 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     7921 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     7981 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     8041 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     8101 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     8161 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     8221 gcgtggttcc tggccaccgt cggagtctcg cccgaccacc agggcaaggg tctgggcagc
     8281 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     8341 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     8401 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaacg
     8461 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     8521 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     8581 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     8641 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     8701 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     8761 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     8821 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     8881 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     8941 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     9001 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     9061 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     9121 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     9181 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     9241 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     9301 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     9361 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     9421 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     9481 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     9541 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     9601 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     9661 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     9721 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     9781 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     9841 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     9901 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     9961 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
    10021 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
    10081 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    10141 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    10201 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    10261 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
    10321 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    10381 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    10441 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    10501 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    10561 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    10621 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    10681 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    10741 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
    10801 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    10861 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    10921 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    10981 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    11041 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    11101 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    11161 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    11221 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    11281 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    11341 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    11401 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    11461 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    11521 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    11581 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    11641 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    11701 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    11761 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    11821 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    11881 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    11941 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    12001 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    12061 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    12121 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    12181 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    12241 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    12301 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    12361 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
    12421 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    12481 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    12541 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
    12601 agct
//