LOCUS       174786_p35u4_pAC        3423 bp ds-DNA     linear   SYN 20-JAN-2022
DEFINITION  .
ACCESSION   urn.local...1628191682521.530
VERSION
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 3, 2017 from SnapGene Viewer 3.1.4 to
            Vector NTI(R) format http://www.snapgene.com
FEATURES             Location/Qualifiers
     CDS             order(complement(<1..219),complement(2983..>3423))
                     /note="cmR"
                     /label="cmR"
     rep_origin      complement(848..1393)
                     /direction="LEFT"
                     /note="ori"
                     /label="ori"
     promoter        1448..1453
                     /label="-35"
     misc_feature    1471..1476
                     /label="-10"
     misc_feature    1494..1499
                     /label="SD"
     CDS             1506..2216
                     /note="lambda cI residues 1-239"
                     /label="lambda cI residues 1-239"
     misc_feature    2217..2225
                     /note="3 Ala linker"
                     /label="3 Ala linker"
     misc_feature    2226..2603
                     /note="MS2 sequence_I actually_got_originally_from_pMalc"
                     /label="MS2cp"
ORIGIN      
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgaatg ctgccaactt actgattagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttcccctg gcggctccct
      961 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     1021 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
     1081 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
     1141 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
     1201 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
     1261 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
     1321 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
     1381 aaacgatctc aagaagatca tcttattaat cagataaaat atttctagat ttcagtgagg
     1441 cctcgagaca gatgctagct cagtcctagg tatagtgcta gcgcatgcca cacaggaaac
     1501 agcgtatgag cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc
     1561 ttaaagcaat ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag
     1621 acaagatggg gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa
     1681 atgcttataa cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc
     1741 cttcaatcgc cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta
     1801 gaagtgagta tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctgagc
     1861 ttagaacctt taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg
     1921 attctgcatt ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc
     1981 caagctttcc tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg
     2041 atttctgcat agccagactt gggggtgatg agtttacctt caagaaactg atcagggata
     2101 gcggtcaggt gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga
     2161 gttgttccgt tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggcgcgg
     2221 ccgcagaatt cccggggatt cacccgggta tggcttctaa ctttactcag ttcgttctcg
     2281 tcgacaatgg cggaactggc gacgtgactg tcgccccaag caacttcgct aacgggatcg
     2341 ctgaatggat cagctctaac tcgcgttcac aggcttacaa agtaacctgt agcgttcgtc
     2401 agagctctgc gcagaatcgc aaatacacca tcaaagtcga ggtgcctaaa ggcgcctggc
     2461 gttcgtactt aaatatggaa ctaaccattc caattttcgc cacgaattcc gactgcgagc
     2521 ttattgttaa ggcaatgcaa ggtctcctaa aagatggaaa cccgattccc tcagcaatcg
     2581 cagcaaactc cggcatctac taaggatcct taccttacag atctgcatcg caggatgctg
     2641 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctaaccgt ttttatcagg
     2701 ctctgggagg cagaataaat gatcatatcg tcaattatta cctccacggg gagagcctga
     2761 gcaaactggc ctcaggcatt tgagaagcac acggtcacac tgcttccggt agtcaataaa
     2821 ccggtaaacc agcaatagac ataagcggct atttaacgac cctgccctga accgacgacc
     2881 gggtcgaatt tgctttcgaa tttctgccat tcatccgctt attatcactt attcaggcgt
     2941 agcaccaggc gtttaagggc accaataact gccttaaaaa aattacgccc cgccctgcca
     3001 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacagac
     3061 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     3121 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     3181 ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg
     3241 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     3301 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     3361 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     3421 acg
//
