LOCUS       174787_pRM16_CW1        3161 bp ds-DNA     linear   SYN 20-JAN-2022
DEFINITION  .
ACCESSION   urn.local...1642709429843.149
VERSION
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 3, 2017 from SnapGene Viewer 3.1.4 to
            Vector NTI(R) format http://www.snapgene.com
FEATURES             Location/Qualifiers
     CDS             order(complement(<1..219),complement(2721..>3161))
                     /note="cmR"
                     /label="cmR"
     rep_origin      complement(848..1393)
                     /direction="LEFT"
                     /note="ori"
                     /label="ori"
     misc_feature    <1562..1566
                     /label="-35"
     misc_feature    1584..1589
                     /label="-10"
     CDS             1619..2329
                     /note="lambda cI residues 1-239"
                     /label="lambda cI residues 1-239"
     misc_feature    2330..2338
                     /note="3 Ala linker"
                     /label="3 Ala linker"
ORIGIN      
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgaatg ctgccaactt actgattagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttcccctg gcggctccct
      961 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     1021 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
     1081 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
     1141 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
     1201 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
     1261 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
     1321 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
     1381 aaacgatctc aagaagatca tcttattaat cagataaaat atttctagat ttcagtgcaa
     1441 tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca
     1501 tgtttgacag cttatcatcg ataagctaat tctcactcat taggcacccc aggcctcgag
     1561 acgatagcta gctcagtcct aggtatagtg ctagcgcatg ccacacagga aacagcgtat
     1621 gagcacaaaa aagaaaccat taacacaaga gcagcttgag gacgcacgtc gccttaaagc
     1681 aatttatgaa aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg cagacaagat
     1741 ggggatgggg cagtcaggcg ttggtgcttt atttaatggc atcaatgcat taaatgctta
     1801 taacgccgca ttgcttgcaa aaattctcaa agttagcgtt gaagaattta gcccttcaat
     1861 cgccagagaa atctacgaga tgtatgaagc ggttagtatg cagccgtcac ttagaagtga
     1921 gtatgagtac cctgtttttt ctcatgttca ggcagggatg ttctcacctg agcttagaac
     1981 ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca gtgattctgc
     2041 attctggctt gaggttgaag gtaattccat gaccgcacca acaggctcca agccaagctt
     2101 tcctgacgga atgttaattc tcgttgaccc tgagcaggct gttgagccag gtgatttctg
     2161 catagccaga cttgggggtg atgagtttac cttcaagaaa ctgatcaggg atagcggtca
     2221 ggtgttttta caaccactaa acccacagta cccaatgatc ccatgcaatg agagttgttc
     2281 cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag acgtttggcg cggccgcata
     2341 aggatcctta ccttacagat ctgcatcgca ggatgctgct ggctaccctg tggaacacct
     2401 acatctgtat taacgaagcg ctaaccgttt ttatcaggct ctgggaggca gaataaatga
     2461 tcatatcgtc aattattacc tccacgggga gagcctgagc aaactggcct caggcatttg
     2521 agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag caatagacat
     2581 aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt
     2641 tctgccattc atccgcttat tatcacttat tcaggcgtag caccaggcgt ttaagggcac
     2701 caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
     2761 tcattaagca ttctgccgac atggaagcca tcacagacgg catgatgaac ctgaatcgcc
     2821 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
     2881 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
     2941 gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
     3001 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
     3061 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca
     3121 ctatcccata tcaccagctc accgtctttc attgccatac g
//
