LOCUS       pPC0667                 8811 bp    DNA     circular UNA 29-JAN-2020
DEFINITION  [pBR322, Cb]
            pCMV-bpNLS-SaCas9(N580A)-(SGGS)2-XTEN-(SGGS)2-MMLV_RT_pentamutant-b
            pNLS.
ACCESSION   urn.local...c-8wnwdza
VERSION     urn.local...c-8wnwdza
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     primer_bind     12..44
                     /Sequence="ACGCCCCCTATTGACGTCAATGACGGTAAATGG"
                     /%GC="51.5"
                     /Transferred_From="NMG-839"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-839"
     primer_bind     219..243
                     /Sequence="GGGAGTTTGTTTTGGCACCAAAATC"
                     /%GC="44.0"
                     /Transferred_From="HRSeq17"
                     /Transferred_Similarity="100.00%"
                     /label="HRSeq17"
     primer_bind     234..253
                     /Sequence="CACCAAAATCAACGGGACTT"
                     /Transferred_From="LCv2_seq1"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq1"
     primer_bind     261..285
                     /Sequence="TGTCGTAACAACTCCGCCCCATTGA"
                     /Hairpin_Tm="30.7"
                     /Tm="66.4"
                     /Self_Dimer_Tm="None"
                     /%GC="52.0"
                     /created_by="primer3"
                     /label="ACK15"
     Promoter        275..356
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="CMV prom"
                     /Transferred_Similarity="97.56%"
                     /label="CMV prom"
     primer_bind     286..306
                     /Sequence="CGCAAATGGGCGGTAGGCGTG"
                     /Transferred_From="Genewiz CMV-Forward"
                     /Transferred_Similarity="100.00%"
                     /label="Genewiz CMV-Forward"
     primer_bind     293..314
                     /Sequence="GGGCGGTAGGCGTGTACGGTGG"
                     /Transferred_From="QB0976 pRLuc-MCS-F"
                     /Transferred_Similarity="100.00%"
                     /label="QB0976 pRLuc-MCS-F"
     primer_bind     complement(331..360)
                     /Sequence="AGCGGATCTGACGGTTCACTAAACCAGCTC"
                     /Transferred_From="352_CMV_Rev2"
                     /Transferred_Similarity="100.00%"
                     /label="352_CMV_Rev2"
     primer_bind     complement(369..403)
                     /Sequence="CGGCTCTCCCTATAGTGAGTCGTATTAGCGGCCGC"
                     /%GC="60.0"
                     /Transferred_From="JRD389"
                     /Transferred_Similarity="100.00%"
                     /label="JRD389"
     primer_bind     complement(369..392)
                     /Sequence="ATAGTGAGUCGTATTAGCGGCCGC"
                     /%GC="54.2"
                     /Transferred_From="JRD184"
                     /Transferred_Similarity="100.00%"
                     /label="JRD184"
     Promoter        377..395
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     primer_bind     377..396
                     /created_by="primer3"
                     /annotation_group="1354740459929005000b9df76f7-7651-430e-8
                     24e-1c833a63d70f"
                     /%GC="40.0"
                     /Tm="50.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TAATACGACTCACTATAGGG"
                     /Transferred_From="T7 promoter"
                     /Transferred_Similarity="100.00%"
                     /label="T7 promoter"
     primer_bind     377..395
                     /Sequence="TAATACGACTCACTATAGG"
                     /%GC="36.8"
                     /Transferred_From
                     /Transferred_Similarity="100.00%"
                     /label
     primer_bind     complement(397..421)
                     /Sequence="CTGTCCGTTTCATGGTGGCGGCTCT"
                     /%GC="60.0"
                     /Transferred_From="JRD416"
                     /Transferred_Similarity="100.00%"
                     /label="JRD416"
     primer_bind     405..423
                     /Sequence="CACCATGAAACGGACAGCC"
                     /%GC="57.9"
                     /Transferred_From="JRD413"
                     /Transferred_Similarity="100.00%"
                     /label="JRD413"
     Misc            409..465
                     /created_by="jessiedavis"
                     /modified_by="jessiedavis"
                     /label="NLS"
     Junction        join(411,466)
                     /Reads_using=6
                     /Reads_supporting_discovery=4
                     /Junction_Type="Deletion"
                     /Deletion_Size=54
                     /Junction_Source="411+"
                     /Junction_Destination="466+"
                     /ID="junction 1"
                     /label="junction 1"
     Junction        join(411,466)
                     /Reads_using=4
                     /Reads_supporting_discovery=4
                     /Junction_Type="Deletion"
                     /Deletion_Size=54
                     /Junction_Source="411+"
                     /Junction_Destination="466+"
                     /ID="junction 1"
                     /label="junction 1"
     misc_feature    412..430
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    432..5816
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     complement(444..465)
                     /Sequence="GACTTTCCGCTTCTTCTTTGGT"
                     /%GC="45.5"
                     /Transferred_From="JRD407"
                     /Transferred_Similarity="100.00%"
                     /label="JRD407"
     primer_bind     445..486
                     /created_by="jessiedavis"
                     /Sequence="CCAAAGAAGAAGCGGAAAGTCGGGAAGCGAAATTACATTCTG"
                     /modified_by="jessiedavis"
                     /label="JRD453"
     primer_bind     complement(447..465)
                     /Sequence="GACTTTCCGCTTCTTCTTT"
                     /Hairpin_Tm="None"
                     /Tm="53.2"
                     /Self_Dimer_Tm="None"
                     /%GC="42.1"
                     /modified_by="jessiedavis"
                     /label="JRD407"
     CDS             466..3624
                     /created_by="thuang"
                     /label="SaCas9"
     primer_bind     489..511
                     /Sequence="GCTGGACATTGGCATTACATCAG"
                     /Hairpin_Tm="None"
                     /Tm="60.2"
                     /Self_Dimer_Tm="None"
                     /%GC="47.8"
                     /created_by="primer3"
                     /label="JRD400"
     modified_base   493..495
                     /created_by="thuang"
                     /modified_by="jessiedavis"
                     /label="D10"
     misc_feature    1174..1176
                     /created_by="jessiedavis"
                     /label="Cys"
     misc_feature    1752^1753
                     /created_by="jessiedavis"
                     /label="Split 430/431, Nishimasu et al"
     misc_feature    1756..1758
                     /created_by="jessiedavis"
                     /label="Mutate to Cys?"
     primer_bind     2040..2055
                     /Sequence="GCTGCACGATATGCAG"
                     /Hairpin_Tm="69.2"
                     /Tm="52.7"
                     /Self_Dimer_Tm="21.7"
                     /%GC="56.3"
                     /modified_by="jessiedavis"
                     /label="JRDSeq8"
     DNA_probe_bind  2064^2065
                     /created_by="jessiedavis"
                     /label="Choice Split Levy"
     misc_feature    2065..2067
                     /created_by="jessiedavis"
                     /label="Cys"
     primer_bind     complement(2194..2212)
                     /Sequence="TCTTGCTGGCCTCCTCCTG"
                     /Hairpin_Tm="None"
                     /Tm="61.3"
                     /Self_Dimer_Tm="3.3"
                     /%GC="63.2"
                     /modified_by="jessiedavis"
                     /label="JRD401"
     primer_bind     2194..2212
                     /Sequence="CAGGAGGAGGCCAGCAAGA"
                     /Hairpin_Tm="None"
                     /Tm="61.3"
                     /Self_Dimer_Tm="5.0"
                     /%GC="63.2"
                     /modified_by="jessiedavis"
                     /label="JRD402"
     Misc            2203..2205
                     /created_by="jessiedavis"
                     /modified_by="jessiedavis"
                     /label="N580A"
     misc_feature    2679^2680
                     /created_by="jessiedavis"
                     /label="Split 739/740, Nishimasu et al"
     DNA_probe_bind  2683..2691
                     /Sequence="AGCATGCCC"
                     /Hairpin_Tm="None"
                     /Tm="29.3"
                     /Self_Dimer_Tm="15.7"
                     /%GC="66.7"
                     /created_by="primer3"
                     /label="Mutate to CFN"
     misc_feature    2683..2685
                     /created_by="jessiedavis"
                     /label="Mutate to Cys?"
     misc_feature    2865^2866
                     /created_by="jessiedavis"
                     /label="Split 801/802"
     misc_feature    3301..3303
                     /created_by="jessiedavis"
                     /label="Cys"
     misc_feature    3301..3303
                     /created_by="jessiedavis"
                     /modified_by="jessiedavis"
                     /label="Tolerates Ala mutation"
     primer_bind     complement(3586..3614)
                     /Sequence="ATAATCTGAGGATGTTTCTTGCTCTTGAC"
                     /Hairpin_Tm="31.8"
                     /Tm="61.7"
                     /Self_Dimer_Tm="None"
                     /%GC="37.9"
                     /modified_by="thuang"
                     /label="TPH722"
     misc_feature    3600..3614
                     /created_by="thuang"
                     /label="USER Junction"
     primer_bind     complement(3600..3624)
                     /Sequence="GCCTTTCTTGATAATCTGAGGATGT"
                     /%GC="40.0"
                     /Transferred_From="JRD403"
                     /Transferred_Similarity="100.00%"
                     /label="JRD403"
     primer_bind     complement(3604..3624)
                     /Sequence="GCCTTTCTTGATAATCTGAGG"
                     /Hairpin_Tm="None"
                     /Tm="54.1"
                     /Self_Dimer_Tm="None"
                     /%GC="42.9"
                     /modified_by="jessiedavis"
                     /label="JRD403"
     primer_bind     3605..3640
                     /Sequence="CTCAGATTATCAAGAAAGGCTCTGGAGGATCTAGCG"
                     /Hairpin_Tm="44.3"
                     /Tm="68.5"
                     /Self_Dimer_Tm="None"
                     /%GC="47.2"
                     /modified_by="jessiedavis"
                     /label="JRD404"
     source          <3625..>3723
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     3625..3644
                     /label="AVA390"
     primer_bind     3625..3644
                     /label="AVA372"
     primer_bind     3625..3644
                     /label="AVA242"
     primer_bind     3657..3677
                     /label="AVA043"
     primer_bind     complement(3697..3723)
                     /label="AVA369"
     primer_bind     complement(3697..3723)
                     /label="AVA262"
     source          <3724..>5754
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             3724..5754
                     /codon_start=1
                     /translation="TLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVR
                     QAPLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVK
                     KPGTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRL
                     HPTSQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFNEALHRDLADFRIQHPDLI
                     LLQYVDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEG
                     QRWLTEARKETVMGQPTPKTPRQLREFLGKAGFCRLFIPGFAEMAAPLYPLTKPGTLF
                     NWGPDQQKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPV
                     AYLSKKLDPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDR
                     WLSNARMTHYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRPD
                     LTDQPLPDADHTWYTDGSSLLQEGQRKAGAAVTTETEVIWAKALPAGTSAQRAELIAL
                     TQALKMAEGKKLNVYTDSRYAFATAHIHGEIYRRRGWLTSEGKEIKNKDEILALLKAL
                     FLPKRLSIIHCPGHQKGHSAEARGNRMADQAARKAAITETPDTSTLLIENSSP"
                     /label="MMLV RT"
     primer_bind     complement(3910..3938)
                     /label="AVA593"
     primer_bind     3929..3948
                     /label="AVA594"
     primer_bind     complement(4002..4019)
                     /label="AVA228"
     primer_bind     complement(4118..4159)
                     /label="AVA479"
     primer_bind     4151..4171
                     /label="AVA480"
     primer_bind     complement(4295..4316)
                     /label="AVA313"
     primer_bind     4306..4340
                     /label="AVA314"
     misc_feature    4321..4323
                     /label="D200N"
     primer_bind     4385..4406
                     /label="AVA006"
     primer_bind     complement(4605..4648)
                     /label="AVA672"
     primer_bind     complement(4625..4649)
                     /label="AVA595"
     primer_bind     complement(4631..4648)
                     /label="AVA675"
     misc_feature    4639..4641
                     /label="T306K"
     primer_bind     4639..4657
                     /label="AVA596"
     primer_bind     4641..4683
                     /label="AVA673"
     primer_bind     4641..4656
                     /label="AVA674"
     misc_feature    4660..4662
                     /label="W313F"
     misc_feature    4693
                     /label="sense mut"
     primer_bind     complement(4693..4719)
                     /label="AVA315"
     primer_bind     4710..4734
                     /label="AVA316"
     misc_feature    4711..4713
                     /label="T330P"
     primer_bind     4984..5004
                     /label="AVA005"
     primer_bind     complement(5002..5021)
                     /label="AVA676"
     primer_bind     5012..5047
                     /label="AVA677"
     primer_bind     complement(5067..5093)
                     /label="AVA679"
     primer_bind     5086..5107
                     /label="AVA678"
     primer_bind     5210..5229
                     /label="AVA007"
     primer_bind     complement(5274..5304)
                     /label="AVA220"
     primer_bind     5296..5319
                     /label="AVA221"
     primer_bind     complement(5502..5526)
                     /label="AVA317"
     primer_bind     5516..5568
                     /label="AVA319"
     primer_bind     5516..5553
                     /label="AVA318"
     misc_feature    5530..5532
                     /label="L603W"
     primer_bind     complement(5724..5749)
                     /label="AVA030"
     primer_bind     5755..5770
                     /Sequence="TCTGGCGGCTCAAAAA"
                     /Hairpin_Tm="None"
                     /Tm="53.3"
                     /Self_Dimer_Tm="11.4"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label="Primer bind"
     CDS             5767..5817
                     /created_by="kzhao"
                     /label="BPNLS"
     primer_bind     5768..5787
                     /Sequence="AAAGAACCGCCGACGGCAGC"
                     /%GC="65.0"
                     /Transferred_From="919_EcoRI-BPNLS_fwd"
                     /Transferred_Similarity="100.00%"
                     /label="919_EcoRI-BPNLS_fwd"
     primer_bind     5818..5841
                     /Sequence="TAACCGGTCATCATCACCATCACC"
                     /Transferred_From="202_bb_NpuN1_F"
                     /Transferred_Similarity="100.00%"
                     /label="202_bb_NpuN1_F"
     primer_bind     5818..5838
                     /Sequence="TAACCGGTCATCATCACCATC"
                     /Transferred_From="129_CMV_fwd"
                     /Transferred_Similarity="100.00%"
                     /label="129_CMV_fwd"
     primer_bind     5820..5837
                     /Sequence="ACCGGTCAUCATCACCAT"
                     /%GC="50.0"
                     /Transferred_From="JRD352"
                     /Transferred_Similarity="100.00%"
                     /label="JRD352"
     primer_bind     5820..5837
                     /Sequence="ACCGGTCATCATCACCAT"
                     /%GC="50.0"
                     /Transferred_From="JRD390"
                     /Transferred_Similarity="100.00%"
                     /label="JRD390"
     primer_bind     5821..5841
                     /Sequence="CCGGTCATCATCACCATCACC"
                     /Transferred_From="468_bb-rev-gfpnls"
                     /Transferred_Similarity="100.00%"
                     /label="468_bb-rev-gfpnls"
     primer_bind     5821..5838
                     /Sequence="CCGGTCATCATCACCATC"
                     /%GC="55.6"
                     /Transferred_From="JRD380"
                     /Transferred_Similarity="100.00%"
                     /label="JRD380"
     primer_bind     5862..5884
                     /Sequence="TCAGCCTCGACTGTGCCTTCTAG"
                     /Transferred_From="304_FP-JL11_F"
                     /Transferred_Similarity="100.00%"
                     /label="304_FP-JL11_F"
     primer_bind     complement(5866..5883)
                     /Sequence="TAGAAGGCACAGTCGAGG"
                     /Transferred_From="QB0008 BGH Reverse"
                     /Transferred_Similarity="100.00%"
                     /label="QB0008 BGH Reverse"
     primer_bind     complement(5866..5883)
                     /Sequence="TAGAAGGCACAGTCGAGG"
                     /Transferred_From="Eton BGH Reverse"
                     /Transferred_Similarity="100.00%"
                     /label="Eton BGH Reverse"
     primer_bind     complement(5866..5883)
                     /Sequence="TAGAAGGCACAGTCGAGG"
                     /Transferred_From="Genewiz BGHR"
                     /Transferred_Similarity="100.00%"
                     /label="Genewiz BGHR"
     Terminator      5869..6096
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="bGH_PA term"
                     /Transferred_Similarity="100.00%"
                     /label="bGH_PA term"
     primer_bind     5869..5889
                     /Sequence="CGACTGTGCCTTCTAGTTGCC"
                     /Transferred_From="435_bGH_QPCRF1"
                     /Transferred_Similarity="100.00%"
                     /label="435_bGH_QPCRF1"
     primer_bind     complement(5869..5889)
                     /Sequence="GGCAACTAGAAGGCACAGTCG"
                     /%GC="57.1"
                     /Transferred_From="JRD371"
                     /Transferred_Similarity="100.00%"
                     /label="JRD371"
     primer_bind     5895..5913
                     /Sequence="TCTGTTGTTTGCCCCTCCC"
                     /Transferred_From="436_bGH_QPCRF2"
                     /Transferred_Similarity="100.00%"
                     /label="436_bGH_QPCRF2"
     misc_feature    complement(6018..6042)
                     /Sequence="CCTTGCTGTCCTGCCCCACCCCACC"
                     /Hairpin_Tm="None"
                     /Tm="73.0"
                     /Self_Dimer_Tm="None"
                     /%GC="72.0"
                     /created_by="primer3"
                     /label="ACK64"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(6018..6042)
                     /Sequence="CCTTGCTGTCCTGCCCCACCCCACC"
                     /Transferred_From="ACK64 (end)"
                     /Transferred_Similarity="100.00%"
                     /label="ACK64 (end)"
     primer_bind     complement(6032..6051)
                     /Sequence="AATCCTCCCCCTTGCTGTCC"
                     /Transferred_From="438_bGH_QPCRR2"
                     /Transferred_Similarity="100.00%"
                     /label="438_bGH_QPCRR2"
     primer_bind     complement(6037..6055)
                     /Sequence="TCCCAATCCTCCCCCTTGC"
                     /Transferred_From="439_bGH_QPCRR3"
                     /Transferred_Similarity="100.00%"
                     /label="439_bGH_QPCRR3"
     primer_bind     complement(6057..6076)
                     /Sequence="CCAGCATGCCTGCTATTGTC"
                     /Transferred_From="437_bGH_QPCRR1"
                     /Transferred_Similarity="100.00%"
                     /label="437_bGH_QPCRR1"
     primer_bind     complement(6077..6096)
                     /Sequence="CCATAGAGCCCACCGCATCC"
                     /%GC="65.0"
                     /Transferred_From="920_bGH-BamHI-rev"
                     /Transferred_Similarity="100.00%"
                     /label="920_bGH-BamHI-rev"
     primer_bind     6155..6181
                     /Sequence="GGCGTAATCATGGTCATAGCTGTTTCC"
                     /Transferred_From="654_CAG_fwd"
                     /Transferred_Similarity="100.00%"
                     /label="654_CAG_fwd"
     primer_bind     6163..6181
                     /Sequence="CATGGTCATAGCTGTTTCC"
                     /%GC="47.4"
                     /Transferred_From="NMG-1452"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1452"
     primer_bind     complement(6167..6183)
                     /created_by="primer3"
                     /annotation_group="135473983514354300085f78ef4-0452-4fae-a
                     bba-93b35302c171"
                     /%GC="47.1"
                     /Tm="49.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CAGGAAACAGCTATGAC"
                     /Transferred_From="M13-R (-26)"
                     /Transferred_Similarity="100.00%"
                     /label="M13-R (-26)"
     primer_bind     complement(6167..6183)
                     /Sequence="CAGGAAACAGCTATGAC"
                     /Transferred_From="Eton M13R Reverse"
                     /Transferred_Similarity="100.00%"
                     /label="Eton M13R Reverse"
     primer_bind     complement(6167..6183)
                     /Sequence="CAGGAAACAGCTATGAC"
                     /Transferred_From="QB0048 M13 Reverse"
                     /Transferred_Similarity="100.00%"
                     /label="QB0048 M13 Reverse"
     primer_bind     complement(6167..6183)
                     /Sequence="CAGGAAACAGCTATGAC"
                     /Transferred_From="Genewiz M13R"
                     /Transferred_Similarity="100.00%"
                     /label="Genewiz M13R"
     primer_bind     complement(6180..6203)
                     /created_by="primer3"
                     /annotation_group="1354739835389783000b8ada402-17c3-46ef-9
                     adc-177f91dab407"
                     /%GC="45.8"
                     /Tm="60.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GAGCGGATAACAATTTCACACAGG"
                     /Transferred_From="M13-R (-46)"
                     /Transferred_Similarity="100.00%"
                     /label="M13-R (-46)"
     primer_bind     complement(6180..6203)
                     /Sequence="GAGCGGATAACAATTTCACACAGG"
                     /Transferred_From="QB0108 Tac promoter"
                     /Transferred_Similarity="100.00%"
                     /label="QB0108 Tac promoter"
     primer_bind     complement(6180..6202)
                     /Sequence="AGCGGATAACAATTTCACACAGG"
                     /Transferred_From="QB0050 M13/pUC Reverse"
                     /Transferred_Similarity="100.00%"
                     /label="QB0050 M13/pUC Reverse"
     Promoter        complement(6216..6245)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lac prom"
                     /Transferred_Similarity="100.00%"
                     /label="lac prom"
     primer_bind     6242..6272
                     /Sequence="TAAAGCCTAGGGTGCCTAATGAGTGAGCTAA"
                     /%GC="45.2"
                     /Transferred_From="NMG-850"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-850"
     primer_bind     complement(6252..6271)
                     /Sequence="TAGCTCACTCATTAGGCACC"
                     /Transferred_From="Lac-F (EtonSeq)"
                     /Transferred_Similarity="100.00%"
                     /label="Lac-F (EtonSeq)"
     primer_bind     complement(6252..6271)
                     /Sequence="TAGCTCACTCATTAGGCACC"
                     /%GC="50.0"
                     /Transferred_From="836_Lac-F"
                     /Transferred_Similarity="100.00%"
                     /label="836_Lac-F"
     misc_feature    complement(6260..6284)
                     /Sequence="AATTAATGTGAGTTAGCTCACTCAT"
                     /Hairpin_Tm="58.9"
                     /Tm="56.6"
                     /Self_Dimer_Tm="15.0"
                     /%GC="32.0"
                     /created_by="primer3"
                     /label="ACK32"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     6260..6284
                     /Sequence="ATGAGTGAGCTAACTCACATTAATT"
                     /Hairpin_Tm="63.4"
                     /Tm="56.6"
                     /Self_Dimer_Tm="30.8"
                     /%GC="32.0"
                     /created_by="primer3"
                     /label="ACK21"
     primer_bind     complement(6260..6284)
                     /Sequence="AATTAATGTGAGTTAGCTCACTCAT"
                     /%GC="32.0"
                     /Transferred_From="ACK-32"
                     /Transferred_Similarity="100.00%"
                     /label="ACK-32"
     primer_bind     6318..6344
                     /Sequence="AACCTGTCGTGCCAGCTGCATTAATGA"
                     /%GC="48.1"
                     /Transferred_From="NMG-1101"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1101"
     primer_bind     complement(6398..6415)
                     /Sequence="AGCGAGTCAGTGAGCGAG"
                     /Transferred_From="QB0039 L4440"
                     /Transferred_Similarity="100.00%"
                     /label="QB0039 L4440"
     primer_bind     complement(6450..6469)
                     /created_by="primer3"
                     /annotation_group="13547385713896020002e37130e-0600-4795-b
                     4cf-db36738ae7a4"
                     /%GC="50.0"
                     /Tm="58.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATTACCGCCTTTGAGTGAGC"
                     /modified_by="User"
                     /Transferred_From="VR"
                     /Transferred_Similarity="100.00%"
                     /label="VR"
     primer_bind     complement(6539..6560)
                     /Sequence="CAACGCGGCCTTTTTACGGTTC"
                     /Transferred_From="11_pFYF_Seq_Rev"
                     /Transferred_Similarity="100.00%"
                     /label="11_pFYF_Seq_Rev"
     misc_feature    complement(6554..7173)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.52%"
                     /label="pBR322 origin"
                     /note="Geneious type: Origin of Replication"
     primer_bind     6569..6593
                     /Sequence="TTTCCATAGGCTCCGCCCCCCTGAC"
                     /Hairpin_Tm="52.7"
                     /Tm="69.9"
                     /Self_Dimer_Tm="None"
                     /%GC="64.0"
                     /created_by="primer3"
                     /label="ACK10"
     primer_bind     complement(6630..6649)
                     /Sequence="TATAGTCCTGTCGGGTTTCG"
                     /Transferred_From="BR322-F"
                     /Transferred_Similarity="100.00%"
                     /label="BR322-F"
     misc_feature    6765
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     6777..6804
                     /Sequence="AGGTATCTCAGTTCGGTGTAGGTCGTTC"
                     /%GC="50.0"
                     /Transferred_From="NMG-851"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-851"
     primer_bind     6792..6810
                     /Sequence="GTGTAGGTCGTTCGCTCCA"
                     /Transferred_From="QB0766 RepO-F"
                     /Transferred_Similarity="100.00%"
                     /label="QB0766 RepO-F"
     primer_bind     complement(6867..6893)
                     /Sequence="TACCGGGTTGGACTCAAGACGATAGTT"
                     /%GC="48.1"
                     /Transferred_From="NMG-309"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-309"
     primer_bind     6889..6908
                     /Sequence="CGGTAAGACACGACTTATCG"
                     /%GC="50.0"
                     /Transferred_From="833_BR322-rev1"
                     /Transferred_Similarity="100.00%"
                     /label="833_BR322-rev1"
     primer_bind     complement(6917..6936)
                     /Sequence="TCCTGTTACCAGTGGCTGCT"
                     /Transferred_From="QB0767 RepO-R"
                     /Transferred_Similarity="100.00%"
                     /label="QB0767 RepO-R"
     misc_feature    7010
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     7070..7094
                     /Sequence="CTCTTGATCCGGCAAACAAACCACC"
                     /Hairpin_Tm="None"
                     /Tm="64.7"
                     /Self_Dimer_Tm="2.9"
                     /%GC="52.0"
                     /created_by="primer3"
                     /label="ACK11"
     primer_bind     complement(7070..7089)
                     /Sequence="TTTGTTTGCCGGATCAAGAG"
                     /%GC="45.0"
                     /Transferred_From="832_BR322-fwd1"
                     /Transferred_Similarity="100.00%"
                     /label="832_BR322-fwd1"
     primer_bind     7320..7348
                     /Sequence="TCTGACAGTTACCAATGCTTAATCAGTGA"
                     /%GC="37.9"
                     /Transferred_From="NMG-853"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-853"
     Marker          complement(7328..8188)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp marker"
                     /Transferred_Similarity="99.88%"
                     /label="amp marker"
     primer_bind     7478..7507
                     /Sequence="CTCACCGGCTCCAGATTTATCAGCAATAAA"
                     /%GC="43.3"
                     /Transferred_From="NMG-584"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-584"
     primer_bind     7569..7593
                     /Sequence="TCTATTAATTGTTGCCGGGAAGCTA"
                     /Hairpin_Tm="None"
                     /Tm="60.4"
                     /Self_Dimer_Tm="9.1"
                     /%GC="40.0"
                     /created_by="primer3"
                     /label="ACK12"
     primer_bind     7677..7703
                     /Sequence="ATGGCTTCATTCAGCTCCGGTTCCCAA"
                     /%GC="51.9"
                     /Transferred_From="NMG-1102"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1102"
     primer_bind     7734..7753
                     /Sequence="TTGTGCAAAAAAGCGGTTAG"
                     /Transferred_From="ACK563"
                     /Transferred_Similarity="100.00%"
                     /label="ACK563"
     primer_bind     7852..7880
                     /Sequence="CCGTAAGATGCTTTTCTGTGACTGGTGAG"
                     /%GC="48.3"
                     /Transferred_From="NMG-854"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-854"
     primer_bind     7951..7970
                     /Sequence="ATAATACCGCGCCACATAGC"
                     /Transferred_From="QB0005 Amp-R"
                     /Transferred_Similarity="100.00%"
                     /label="QB0005 Amp-R"
     primer_bind     8012..8041
                     /Sequence="GCGAAAACTCTCAAGGATCTTACCGCTGTT"
                     /%GC="46.7"
                     /Transferred_From="NMG-585"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-585"
     primer_bind     8036..8063
                     /Sequence="GCTGTTGAGATCCAGTTCGATGTAACCC"
                     /Transferred_From="563_Amp_Rev"
                     /Transferred_Similarity="100.00%"
                     /label="563_Amp_Rev"
     primer_bind     complement(8036..8063)
                     /Sequence="GGGTTACATCGAACTGGATCTCAACAGC"
                     /Transferred_From="562_Amp_Fwd"
                     /Transferred_Similarity="100.00%"
                     /label="562_Amp_Fwd"
     primer_bind     8069..8093
                     /Sequence="TGCACCCAACTGATCTTCAGCATCT"
                     /Hairpin_Tm="50.2"
                     /Tm="64.5"
                     /Self_Dimer_Tm="None"
                     /%GC="48.0"
                     /created_by="primer3"
                     /label="ACK13"
     primer_bind     8170..8199
                     /Sequence="GGAAATGTTGAATACTCATACTCTTCCTTT"
                     /%GC="33.3"
                     /Transferred_From="NMG-396"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-396"
     primer_bind     8227..8249
                     /Sequence="GTTATTGTCTCATGAGCGGATAC"
                     /%GC="43.5"
                     /Transferred_From="NMG-1469"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1469"
     Promoter        complement(8230..8258)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="100.00%"
                     /label="amp prom"
     primer_bind     8243..8269
                     /Sequence="CGGATACATATTTGAATGTATTTAGAA"
                     /%GC="25.9"
                     /Transferred_From="NMG-1103"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1103"
     primer_bind     8302..8319
                     /Sequence="CCCGAAAAGTGCCACCTG"
                     /Transferred_From="QB0090 pQE promoter"
                     /Transferred_Similarity="100.00%"
                     /label="QB0090 pQE promoter"
     primer_bind     8309..8337
                     /Sequence="AGTGCCACCTGACGTCGACGGATCGGGAG"
                     /%GC="65.5"
                     /Transferred_From="NMG-838"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-838"
     primer_bind     complement(8372..8391)
                     /Sequence="CGGCATCAGAGCAGATTGTA"
                     /Transferred_From="QB0092 pRS-marker"
                     /Transferred_Similarity="100.00%"
                     /label="QB0092 pRS-marker"
     primer_bind     8572..8596
                     /Sequence="TATTGACTAGTTATTAATAGTAATC"
                     /Hairpin_Tm="32.1"
                     /Tm="48.0"
                     /Self_Dimer_Tm="5.9"
                     /%GC="20.0"
                     /created_by="primer3"
                     /label="ACK14"
     primer_bind     8572..8596
                     /Sequence="TATTGACTAGTTATTAATAGTAATC"
                     /Transferred_From="JLseq006_ACK14"
                     /Transferred_Similarity="100.00%"
                     /label="JLseq006_ACK14"
     promoter        8579..356
                     /created_by="User"
                     /modified_by="User"
                     /Transferred_From="CMV_Full"
                     /Transferred_Similarity="99.83%"
                     /label="CMV_Full"
     primer_bind     8579..8622
                     /Sequence="TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCC"
                     /Transferred_From="387_pAAV_fwd"
                     /Transferred_Similarity="100.00%"
                     /label="387_pAAV_fwd"
     primer_bind     complement(8629..8648)
                     /Sequence="TTATGTAACGCGGAACTCCA"
                     /Transferred_From="QB1110 pSicoR"
                     /Transferred_Similarity="100.00%"
                     /label="QB1110 pSicoR"
     misc_feature    8768
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
ORIGIN      
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcgggaa gcgaaattac
      481 attctggggc tggacattgg cattacatca gtgggctatg gcatcattga ctacgagaca
      541 agggacgtga tcgacgccgg cgtgagactg ttcaaggagg ccaacgtgga gaacaatgag
      601 ggccggagat ccaagagggg agcaaggcgc ctgaagcgga gaaggcgcca cagaatccag
      661 agagtgaaga agctgctgtt cgattacaac ctgctgaccg accactccga gctgtctggc
      721 atcaatcctt atgaggccag agtgaagggc ctgtcccaga agctgtctga ggaggagttt
      781 agcgccgccc tgctgcacct ggcaaagagg agaggcgtgc acaacgtgaa tgaggtggag
      841 gaggacaccg gcaacgagct gtccacaaag gagcagatca gccgcaattc caaggccctg
      901 gaggagaagt atgtggccga gctgcagctg gagcggctga agaaggatgg cgaggtgagg
      961 ggctccatca atcgcttcaa gacctctgac tacgtgaagg aggccaagca gctgctgaag
     1021 gtgcagaagg cctaccacca gctggatcag tcctttatcg atacatatat cgacctgctg
     1081 gagacaaggc gcacatacta tgagggacca ggagagggct ctcccttcgg ctggaaggac
     1141 atcaaggagt ggtacgagat gctgatgggc cactgcacct attttccaga ggagctgaga
     1201 agcgtgaagt acgcctataa cgccgatctg tacaacgccc tgaatgacct gaacaacctg
     1261 gtcatcacca gggatgagaa cgagaagctg gagtactatg agaagttcca gatcatcgag
     1321 aacgtgttca agcagaagaa gaagcctaca ctgaagcaga tcgccaagga gatcctggtg
     1381 aacgaggagg acatcaaggg ctaccgcgtg acctccacag gcaagccaga gttcaccaat
     1441 ctgaaggtgt atcacgatat caaggacatc acagcccgga aggagatcat cgagaacgcc
     1501 gagctgctgg atcagatcgc caagatcctg accatctatc agagctccga ggacatccag
     1561 gaggagctga ccaacctgaa tagcgagctg acacaggagg agatcgagca gatcagcaat
     1621 ctgaagggct acaccggcac acacaacctg agcctgaagg ccatcaatct gatcctggat
     1681 gagctgtggc acacaaacga caatcagatc gccatcttta accggctgaa gctggtgcca
     1741 aagaaggtgg acctgtccca gcagaaggag atcccaacca cactggtgga cgatttcatc
     1801 ctgtctcccg tggtgaagcg gagcttcatc cagagcatca aagtgatcaa cgccatcatc
     1861 aagaagtacg gcctgcccaa tgatatcatc atcgagctgg ccagggagaa gaactccaag
     1921 gacgcccaga agatgatcaa tgagatgcag aagaggaacc gccagaccaa tgagcggatc
     1981 gaggagatca tcagaaccac aggcaaggag aacgccaagt acctgatcga gaagatcaag
     2041 ctgcacgata tgcaggaggg caagtgtctg tattctctgg aggccatccc tctggaggac
     2101 ctgctgaaca atccattcaa ctacgaggtg gatcacatca tcccccggag cgtgagcttc
     2161 gacaattctt ttaacaataa ggtgctggtg aagcaggagg aggccagcaa gaagggcaat
     2221 aggacccctt tccagtacct gtctagctcc gattctaaga tcagctacga gacattcaag
     2281 aagcacatcc tgaatctggc caagggcaag ggccgcatca gcaagaccaa gaaggagtac
     2341 ctgctggagg agcgggacat caacagattc tccgtgcaga aggacttcat caaccggaat
     2401 ctggtggaca ccagatacgc cacacgcggc ctgatgaatc tgctgcggtc ttatttcaga
     2461 gtgaacaatc tggatgtgaa ggtgaagagc atcaacggcg gcttcacctc ctttctgcgg
     2521 agaaagtgga agtttaagaa ggagcgcaac aagggctata agcaccacgc cgaggatgcc
     2581 ctgatcatcg ccaatgccga cttcatcttt aaggagtgga agaagctgga caaggccaag
     2641 aaagtgatgg agaaccagat gttcgaggag aagcaggccg agagcatgcc cgagatcgag
     2701 acagagcagg agtacaagga gattttcatc acacctcacc agatcaagca catcaaggac
     2761 ttcaaggact acaagtattc tcacagggtg gataagaagc ccaaccgcga gctgatcaat
     2821 gacaccctgt atagcacacg gaaggacgat aagggcaata ccctgatcgt gaacaatctg
     2881 aacggcctgt acgacaagga taatgacaag ctgaagaagc tgatcaacaa gtctcccgag
     2941 aagctgctga tgtaccacca cgatcctcag acatatcaga agctgaagct gatcatggag
     3001 cagtacggcg acgagaagaa cccactgtat aagtactatg aggagacagg caactacctg
     3061 acaaagtata gcaagaagga taatggcccc gtgatcaaga agatcaagta ctatggcaac
     3121 aagctgaatg cccacctgga catcaccgac gattacccta actctcgcaa taaggtggtg
     3181 aagctgagcc tgaagccata ccggttcgac gtgtacctgg acaacggcgt gtataagttt
     3241 gtgacagtga agaatctgga tgtgatcaag aaggagaact actatgaggt gaacagcaag
     3301 tgctacgagg aggccaagaa gctgaagaag atcagcaacc aggccgagtt catcgcctct
     3361 ttttacaaca atgacctgat caagatcaat ggcgagctgt atagagtgat cggcgtgaac
     3421 aatgatctgc tgaacagaat cgaagtgaat atgatcgaca tcacctacag ggagtatctg
     3481 gagaacatga atgataagag gccccctcgc atcatcaaga ccatcgcctc taagacacag
     3541 agcatcaaga agtacagcac agacatcctg gggaacctgt atgaagtcaa gagcaagaaa
     3601 catcctcaga ttatcaagaa aggctctgga ggatctagcg gaggatcctc tggcagcgag
     3661 acaccaggaa caagcgagtc agcaacacca gagagcagtg gcggcagcag cggcggcagc
     3721 agcaccctaa atatagaaga tgagtatcgg ctacatgaga cctcaaaaga gccagatgtt
     3781 tctctagggt ccacatggct gtctgatttt cctcaggcct gggcggaaac cgggggcatg
     3841 ggactggcag ttcgccaagc tcctctgatc atacctctga aagcaacctc tacccccgtg
     3901 tccataaaac aataccccat gtcacaagaa gccagactgg ggatcaagcc ccacatacag
     3961 agactgttgg accagggaat actggtaccc tgccagtccc cctggaacac gcccctgcta
     4021 cccgttaaga aaccagggac taatgattat aggcctgtcc aggatctgag agaagtcaac
     4081 aagcgggtgg aagacatcca ccccaccgtg cccaaccctt acaacctctt gagcgggctc
     4141 ccaccgtccc accagtggta cactgtgctt gatttaaagg atgccttttt ctgcctgaga
     4201 ctccacccca ccagtcagcc tctcttcgcc tttgagtgga gagatccaga gatgggaatc
     4261 tcaggacaat tgacctggac cagactccca cagggtttca aaaacagtcc caccctgttt
     4321 aatgaggcac tgcacagaga cctagcagac ttccggatcc agcacccaga cttgatcctg
     4381 ctacagtacg tggatgactt actgctggcc gccacttctg agctagactg ccaacaaggt
     4441 actcgggccc tgttacaaac cctagggaac ctcgggtatc gggcctcggc caagaaagcc
     4501 caaatttgcc agaaacaggt caagtatctg gggtatcttc taaaagaggg tcagagatgg
     4561 ctgactgagg ccagaaaaga gactgtgatg gggcagccta ctccgaagac ccctcgacaa
     4621 ctaagggagt tcctagggaa ggcaggcttc tgtcgcctct tcatccctgg gtttgcagaa
     4681 atggcagccc ccctgtaccc tctcaccaaa ccggggactc tgtttaattg gggcccagac
     4741 caacaaaagg cctatcaaga aatcaagcaa gctcttctaa ctgccccagc cctggggttg
     4801 ccagatttga ctaagccctt tgaactcttt gtcgacgaga agcagggcta cgccaaaggt
     4861 gtcctaacgc aaaaactggg accttggcgt cggccggtgg cctacctgtc caaaaagcta
     4921 gacccagtag cagctgggtg gcccccttgc ctacggatgg tagcagccat tgccgtactg
     4981 acaaaggatg caggcaagct aaccatggga cagccactag tcattctggc cccccatgca
     5041 gtagaggcac tagtcaaaca accccccgac cgctggcttt ccaacgcccg gatgactcac
     5101 tatcaggcct tgcttttgga cacggaccgg gtccagttcg gaccggtggt agccctgaac
     5161 ccggctacgc tgctcccact gcctgaggaa gggctgcaac acaactgcct tgatatcctg
     5221 gccgaagccc acggaacccg acccgaccta acggaccagc cgctcccaga cgccgaccac
     5281 acctggtaca cggatggaag cagtctctta caagagggac agcgtaaggc gggagctgcg
     5341 gtgaccaccg agaccgaggt aatctgggct aaagccctgc cagccgggac atccgctcag
     5401 cgggctgaac tgatagcact cacccaggcc ctaaagatgg cagaaggtaa gaagctaaat
     5461 gtttatactg atagccgtta tgcttttgct actgcccata tccatggaga aatatacaga
     5521 aggcgtgggt ggctcacatc agaaggcaaa gagatcaaaa ataaagacga gatcttggcc
     5581 ctactaaaag ccctctttct gcccaaaaga cttagcataa tccattgtcc aggacatcaa
     5641 aagggacaca gcgccgaggc tagaggcaac cggatggctg accaagcggc ccgaaaggca
     5701 gccatcacag agactccaga cacctctacc ctcctcatag aaaattcatc accctctggc
     5761 ggctcaaaaa gaaccgccga cggcagcgaa ttcgagccca agaagaagag gaaagtctaa
     5821 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
     5881 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     5941 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     6001 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     6061 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     6121 ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6181 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6241 gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6301 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6361 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6421 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6481 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6541 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6601 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6661 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6721 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6781 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6841 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6901 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6961 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7021 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7081 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7141 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7201 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7261 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7321 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7381 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7441 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7501 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7561 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7621 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7681 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7741 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7801 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7861 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7921 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7981 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8041 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8101 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8161 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8221 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8281 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     8341 gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc gcatagttaa
     8401 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     8461 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
     8521 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
     8581 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     8641 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     8701 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     8761 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat c
//
