LOCUS       pPC1071                 9273 bp    DNA     circular UNA 15-JUN-2021
DEFINITION  [pBR322, Cb]
            pCMV-bpNLS-SaCas9(N580A)-(SGGS)2-XTEN-(SGGS)2-MMLV_RT_pentamutant-b
            pNLS-P2A-BSD.
ACCESSION   urn.local...c-8wnwdza
VERSION     urn.local...c-8wnwdza
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     primer_bind     12..44
                     /Sequence="ACGCCCCCTATTGACGTCAATGACGGTAAATGG"
                     /%GC="51.5"
                     /Transferred_From="NMG-839"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-839"
     primer_bind     219..243
                     /Sequence="GGGAGTTTGTTTTGGCACCAAAATC"
                     /%GC="44.0"
                     /Transferred_From="HRSeq17"
                     /Transferred_Similarity="100.00%"
                     /label="HRSeq17"
     primer_bind     234..253
                     /Sequence="CACCAAAATCAACGGGACTT"
                     /Transferred_From="LCv2_seq1"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq1"
     primer_bind     261..285
                     /Sequence="TGTCGTAACAACTCCGCCCCATTGA"
                     /Hairpin_Tm="30.7"
                     /Tm="66.4"
                     /Self_Dimer_Tm="None"
                     /%GC="52.0"
                     /created_by="primer3"
                     /label="ACK15"
     Promoter        275..356
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="CMV prom"
                     /Transferred_Similarity="97.56%"
                     /label="CMV prom"
     primer_bind     286..306
                     /Sequence="CGCAAATGGGCGGTAGGCGTG"
                     /Transferred_From="Genewiz CMV-Forward"
                     /Transferred_Similarity="100.00%"
                     /label="Genewiz CMV-Forward"
     primer_bind     293..314
                     /Sequence="GGGCGGTAGGCGTGTACGGTGG"
                     /Transferred_From="QB0976 pRLuc-MCS-F"
                     /Transferred_Similarity="100.00%"
                     /label="QB0976 pRLuc-MCS-F"
     primer_bind     complement(310..338)
                     /Sequence="ACCAGCTCTGCUTATATAGACCTCCCACC"
                     /%GC="51.7"
                     /Transferred_From="PC0767"
                     /Transferred_Similarity="100.00%"
                     /label="PC0767"
     primer_bind     327..353
                     /Sequence="AGCAGAGCTGGUTTAGTGAACCGTCAG"
                     /%GC="51.9"
                     /Transferred_From="PC0766"
                     /Transferred_Similarity="100.00%"
                     /label="PC0766"
     primer_bind     complement(331..360)
                     /Sequence="AGCGGATCTGACGGTTCACTAAACCAGCTC"
                     /Transferred_From="352_CMV_Rev2"
                     /Transferred_Similarity="100.00%"
                     /label="352_CMV_Rev2"
     primer_bind     complement(369..403)
                     /Sequence="CGGCTCTCCCTATAGTGAGTCGTATTAGCGGCCGC"
                     /%GC="60.0"
                     /Transferred_From="JRD389"
                     /Transferred_Similarity="100.00%"
                     /label="JRD389"
     primer_bind     complement(369..392)
                     /Sequence="ATAGTGAGUCGTATTAGCGGCCGC"
                     /%GC="54.2"
                     /Transferred_From="JRD184"
                     /Transferred_Similarity="100.00%"
                     /label="JRD184"
     Promoter        377..395
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     primer_bind     377..396
                     /created_by="primer3"
                     /annotation_group="1354740459929005000b9df76f7-7651-430e-8
                     24e-1c833a63d70f"
                     /%GC="40.0"
                     /Tm="50.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TAATACGACTCACTATAGGG"
                     /Transferred_From="T7 promoter"
                     /Transferred_Similarity="100.00%"
                     /label="T7 promoter"
     primer_bind     377..395
                     /Sequence="TAATACGACTCACTATAGG"
                     /%GC="36.8"
                     /Transferred_From
                     /Transferred_Similarity="100.00%"
                     /label
     primer_bind     complement(397..421)
                     /Sequence="CTGTCCGTTTCATGGTGGCGGCTCT"
                     /%GC="60.0"
                     /Transferred_From="JRD416"
                     /Transferred_Similarity="100.00%"
                     /label="JRD416"
     primer_bind     405..423
                     /Sequence="CACCATGAAACGGACAGCC"
                     /%GC="57.9"
                     /Transferred_From="JRD413"
                     /Transferred_Similarity="100.00%"
                     /label="JRD413"
     Misc            409..465
                     /created_by="jessiedavis"
                     /modified_by="jessiedavis"
                     /label="NLS"
     Junction        join(411,466)
                     /Reads_using=6
                     /Reads_supporting_discovery=4
                     /Junction_Type="Deletion"
                     /Deletion_Size=54
                     /Junction_Source="411+"
                     /Junction_Destination="466+"
                     /ID="junction 1"
                     /label="junction 1"
     Junction        join(411,466)
                     /Reads_using=4
                     /Reads_supporting_discovery=4
                     /Junction_Type="Deletion"
                     /Deletion_Size=54
                     /Junction_Source="411+"
                     /Junction_Destination="466+"
                     /ID="junction 1"
                     /label="junction 1"
     misc_feature    412..430
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     complement(444..465)
                     /Sequence="GACTTTCCGCTTCTTCTTTGGT"
                     /%GC="45.5"
                     /Transferred_From="JRD407"
                     /Transferred_Similarity="100.00%"
                     /label="JRD407"
     primer_bind     445..486
                     /created_by="jessiedavis"
                     /Sequence="CCAAAGAAGAAGCGGAAAGTCGGGAAGCGAAATTACATTCTG"
                     /modified_by="jessiedavis"
                     /label="JRD453"
     primer_bind     complement(447..465)
                     /Sequence="GACTTTCCGCTTCTTCTTT"
                     /Hairpin_Tm="None"
                     /Tm="53.2"
                     /Self_Dimer_Tm="None"
                     /%GC="42.1"
                     /modified_by="jessiedavis"
                     /label="JRD407"
     CDS             466..3624
                     /created_by="thuang"
                     /label="SaCas9"
     primer_bind     489..511
                     /Sequence="GCTGGACATTGGCATTACATCAG"
                     /Hairpin_Tm="None"
                     /Tm="60.2"
                     /Self_Dimer_Tm="None"
                     /%GC="47.8"
                     /created_by="primer3"
                     /label="JRD400"
     modified_base   493..495
                     /created_by="thuang"
                     /modified_by="jessiedavis"
                     /label="D10"
     misc_feature    1174..1176
                     /created_by="jessiedavis"
                     /label="Cys"
     misc_feature    1752^1753
                     /created_by="jessiedavis"
                     /label="Split 430/431, Nishimasu et al"
     misc_feature    1756..1758
                     /created_by="jessiedavis"
                     /label="Mutate to Cys?"
     primer_bind     2040..2055
                     /Sequence="GCTGCACGATATGCAG"
                     /Hairpin_Tm="69.2"
                     /Tm="52.7"
                     /Self_Dimer_Tm="21.7"
                     /%GC="56.3"
                     /modified_by="jessiedavis"
                     /label="JRDSeq8"
     DNA_probe_bind  2064^2065
                     /created_by="jessiedavis"
                     /label="Choice Split Levy"
     misc_feature    2065..2067
                     /created_by="jessiedavis"
                     /label="Cys"
     primer_bind     complement(2194..2212)
                     /Sequence="TCTTGCTGGCCTCCTCCTG"
                     /Hairpin_Tm="None"
                     /Tm="61.3"
                     /Self_Dimer_Tm="3.3"
                     /%GC="63.2"
                     /modified_by="jessiedavis"
                     /label="JRD401"
     primer_bind     2194..2212
                     /Sequence="CAGGAGGAGGCCAGCAAGA"
                     /Hairpin_Tm="None"
                     /Tm="61.3"
                     /Self_Dimer_Tm="5.0"
                     /%GC="63.2"
                     /modified_by="jessiedavis"
                     /label="JRD402"
     Misc            2203..2205
                     /created_by="jessiedavis"
                     /modified_by="jessiedavis"
                     /label="N580A"
     misc_feature    2679^2680
                     /created_by="jessiedavis"
                     /label="Split 739/740, Nishimasu et al"
     DNA_probe_bind  2683..2691
                     /Sequence="AGCATGCCC"
                     /Hairpin_Tm="None"
                     /Tm="29.3"
                     /Self_Dimer_Tm="15.7"
                     /%GC="66.7"
                     /created_by="primer3"
                     /label="Mutate to CFN"
     misc_feature    2683..2685
                     /created_by="jessiedavis"
                     /label="Mutate to Cys?"
     misc_feature    2865^2866
                     /created_by="jessiedavis"
                     /label="Split 801/802"
     misc_feature    3301..3303
                     /created_by="jessiedavis"
                     /label="Cys"
     misc_feature    3301..3303
                     /created_by="jessiedavis"
                     /modified_by="jessiedavis"
                     /label="Tolerates Ala mutation"
     primer_bind     complement(3586..3614)
                     /Sequence="ATAATCTGAGGATGTTTCTTGCTCTTGAC"
                     /Hairpin_Tm="31.8"
                     /Tm="61.7"
                     /Self_Dimer_Tm="None"
                     /%GC="37.9"
                     /modified_by="thuang"
                     /label="TPH722"
     misc_feature    3600..3614
                     /created_by="thuang"
                     /label="USER Junction"
     primer_bind     complement(3600..3624)
                     /Sequence="GCCTTTCTTGATAATCTGAGGATGT"
                     /%GC="40.0"
                     /Transferred_From="JRD403"
                     /Transferred_Similarity="100.00%"
                     /label="JRD403"
     primer_bind     complement(3604..3624)
                     /Sequence="GCCTTTCTTGATAATCTGAGG"
                     /Hairpin_Tm="None"
                     /Tm="54.1"
                     /Self_Dimer_Tm="None"
                     /%GC="42.9"
                     /modified_by="jessiedavis"
                     /label="JRD403"
     primer_bind     3605..3640
                     /Sequence="CTCAGATTATCAAGAAAGGCTCTGGAGGATCTAGCG"
                     /Hairpin_Tm="44.3"
                     /Tm="68.5"
                     /Self_Dimer_Tm="None"
                     /%GC="47.2"
                     /modified_by="jessiedavis"
                     /label="JRD404"
     source          <3625..>3723
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     3625..3644
                     /label="AVA390"
     primer_bind     3625..3644
                     /label="AVA372"
     primer_bind     3625..3644
                     /label="AVA242"
     primer_bind     3657..3677
                     /label="AVA043"
     primer_bind     complement(3697..3723)
                     /label="AVA369"
     primer_bind     complement(3697..3723)
                     /label="AVA262"
     CDS             3724..5754
                     /codon_start=1
                     /translation="TLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVR
                     QAPLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVK
                     KPGTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRL
                     HPTSQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFNEALHRDLADFRIQHPDLI
                     LLQYVDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEG
                     QRWLTEARKETVMGQPTPKTPRQLREFLGKAGFCRLFIPGFAEMAAPLYPLTKPGTLF
                     NWGPDQQKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPV
                     AYLSKKLDPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDR
                     WLSNARMTHYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRPD
                     LTDQPLPDADHTWYTDGSSLLQEGQRKAGAAVTTETEVIWAKALPAGTSAQRAELIAL
                     TQALKMAEGKKLNVYTDSRYAFATAHIHGEIYRRRGWLTSEGKEIKNKDEILALLKAL
                     FLPKRLSIIHCPGHQKGHSAEARGNRMADQAARKAAITETPDTSTLLIENSSP"
                     /label="MMLV RT"
     primer_bind     complement(3910..3938)
                     /label="AVA593"
     primer_bind     3929..3948
                     /label="AVA594"
     primer_bind     complement(4002..4019)
                     /label="AVA228"
     primer_bind     complement(4118..4159)
                     /label="AVA479"
     primer_bind     4151..4171
                     /label="AVA480"
     primer_bind     complement(4295..4316)
                     /label="AVA313"
     primer_bind     4306..4340
                     /label="AVA314"
     misc_feature    4321..4323
                     /label="D200N"
     primer_bind     4385..4406
                     /label="AVA006"
     primer_bind     complement(4605..4648)
                     /label="AVA672"
     primer_bind     complement(4625..4649)
                     /label="AVA595"
     primer_bind     complement(4631..4648)
                     /label="AVA675"
     misc_feature    4639..4641
                     /label="T306K"
     primer_bind     4639..4657
                     /label="AVA596"
     primer_bind     4641..4683
                     /label="AVA673"
     primer_bind     4641..4656
                     /label="AVA674"
     misc_feature    4660..4662
                     /label="W313F"
     misc_feature    4693
                     /label="sense mut"
     primer_bind     complement(4693..4719)
                     /label="AVA315"
     primer_bind     4710..4734
                     /label="AVA316"
     misc_feature    4711..4713
                     /label="T330P"
     primer_bind     4984..5004
                     /label="AVA005"
     primer_bind     complement(5002..5021)
                     /label="AVA676"
     primer_bind     5012..5047
                     /label="AVA677"
     primer_bind     complement(5067..5093)
                     /label="AVA679"
     primer_bind     5086..5107
                     /label="AVA678"
     primer_bind     5210..5229
                     /label="AVA007"
     primer_bind     complement(5274..5304)
                     /label="AVA220"
     primer_bind     5296..5319
                     /label="AVA221"
     primer_bind     complement(5502..5526)
                     /label="AVA317"
     primer_bind     5516..5568
                     /label="AVA319"
     primer_bind     5516..5553
                     /label="AVA318"
     misc_feature    5530..5532
                     /label="L603W"
     primer_bind     complement(5724..5749)
                     /label="AVA030"
     primer_bind     5755..5770
                     /Sequence="TCTGGCGGCTCAAAAA"
                     /Hairpin_Tm="None"
                     /Tm="53.3"
                     /Self_Dimer_Tm="11.4"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label="Primer bind"
     CDS             5767..5817
                     /created_by="kzhao"
                     /label="BPNLS"
     primer_bind     5768..5787
                     /Sequence="AAAGAACCGCCGACGGCAGC"
                     /%GC="65.0"
                     /Transferred_From="919_EcoRI-BPNLS_fwd"
                     /Transferred_Similarity="100.00%"
                     /label="919_EcoRI-BPNLS_fwd"
     primer_bind     complement(5788..5821)
                     /Sequence="ATCCGACTTTCCTCTTCTTCTTGGGCTCGAATTC"
                     /Tm="69.2"
                     /%GC="47.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1028"
     USER            5809..5821
                     /created_by="chenp"
     primer_bind     5809..5838
                     /Sequence="AGGAAAGTCGGATCCGGCGCAACAAACTTC"
                     /Tm="70.7"
                     /%GC="53.3"
                     /Hairpin_Tm="49.7"
                     /Self_Dimer_Tm="31.5"
                     /created_by="chenp"
                     /label="PC1027"
     misc_feature    5818..6279
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     CDS             5827..5883
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding
                     separatepolypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             5884..>6279
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
                     GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHP
                     GIKAIVKDSDGQPTAVGIRELLPSGYVWEG"
                     /label="BSD"
     primer_bind     complement(6260..6287)
                     /Sequence="ACCGGTTAGCCCTCCCACACATAACCAG"
                     /Tm="69.5"
                     /%GC="57.1"
                     /Hairpin_Tm="40.3"
                     /Self_Dimer_Tm="3.3"
                     /created_by="chenp"
                     /label="PC1030"
     USER            6275..6287
                     /created_by="chenp"
     primer_bind     6275..6303
                     /created_by="chenp"
                     /Sequence="AGGGCTAACCGGTCATCATCACCATCACC"
                     /Tm="69.5"
                     /%GC="55.2"
                     /Hairpin_Tm="47.0"
                     /Self_Dimer_Tm="8.0"
                     /modified_by="chenp"
                     /label="PC1029"
     primer_bind     6280..6303
                     /Sequence="TAACCGGTCATCATCACCATCACC"
                     /Transferred_From="202_bb_NpuN1_F"
                     /Transferred_Similarity="100.00%"
                     /label="202_bb_NpuN1_F"
     primer_bind     6280..6300
                     /Sequence="TAACCGGTCATCATCACCATC"
                     /Transferred_From="129_CMV_fwd"
                     /Transferred_Similarity="100.00%"
                     /label="129_CMV_fwd"
     primer_bind     6282..6299
                     /Sequence="ACCGGTCAUCATCACCAT"
                     /%GC="50.0"
                     /Transferred_From="JRD352"
                     /Transferred_Similarity="100.00%"
                     /label="JRD352"
     primer_bind     6282..6299
                     /Sequence="ACCGGTCATCATCACCAT"
                     /%GC="50.0"
                     /Transferred_From="JRD390"
                     /Transferred_Similarity="100.00%"
                     /label="JRD390"
     primer_bind     6283..6303
                     /Sequence="CCGGTCATCATCACCATCACC"
                     /Transferred_From="468_bb-rev-gfpnls"
                     /Transferred_Similarity="100.00%"
                     /label="468_bb-rev-gfpnls"
     primer_bind     6283..6300
                     /Sequence="CCGGTCATCATCACCATC"
                     /%GC="55.6"
                     /Transferred_From="JRD380"
                     /Transferred_Similarity="100.00%"
                     /label="JRD380"
     primer_bind     6324..6346
                     /Sequence="TCAGCCTCGACTGTGCCTTCTAG"
                     /Transferred_From="304_FP-JL11_F"
                     /Transferred_Similarity="100.00%"
                     /label="304_FP-JL11_F"
     primer_bind     complement(6328..6345)
                     /Sequence="TAGAAGGCACAGTCGAGG"
                     /Transferred_From="QB0008 BGH Reverse"
                     /Transferred_Similarity="100.00%"
                     /label="QB0008 BGH Reverse"
     primer_bind     complement(6328..6345)
                     /Sequence="TAGAAGGCACAGTCGAGG"
                     /Transferred_From="Eton BGH Reverse"
                     /Transferred_Similarity="100.00%"
                     /label="Eton BGH Reverse"
     primer_bind     complement(6328..6345)
                     /Sequence="TAGAAGGCACAGTCGAGG"
                     /Transferred_From="Genewiz BGHR"
                     /Transferred_Similarity="100.00%"
                     /label="Genewiz BGHR"
     Terminator      6331..6558
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="bGH_PA term"
                     /Transferred_Similarity="100.00%"
                     /label="bGH_PA term"
     primer_bind     6331..6351
                     /Sequence="CGACTGTGCCTTCTAGTTGCC"
                     /Transferred_From="435_bGH_QPCRF1"
                     /Transferred_Similarity="100.00%"
                     /label="435_bGH_QPCRF1"
     primer_bind     complement(6331..6351)
                     /Sequence="GGCAACTAGAAGGCACAGTCG"
                     /%GC="57.1"
                     /Transferred_From="JRD371"
                     /Transferred_Similarity="100.00%"
                     /label="JRD371"
     primer_bind     6357..6375
                     /Sequence="TCTGTTGTTTGCCCCTCCC"
                     /Transferred_From="436_bGH_QPCRF2"
                     /Transferred_Similarity="100.00%"
                     /label="436_bGH_QPCRF2"
     misc_feature    complement(6480..6504)
                     /Sequence="CCTTGCTGTCCTGCCCCACCCCACC"
                     /Hairpin_Tm="None"
                     /Tm="73.0"
                     /Self_Dimer_Tm="None"
                     /%GC="72.0"
                     /created_by="primer3"
                     /label="ACK64"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(6480..6504)
                     /Sequence="CCTTGCTGTCCTGCCCCACCCCACC"
                     /Transferred_From="ACK64 (end)"
                     /Transferred_Similarity="100.00%"
                     /label="ACK64 (end)"
     primer_bind     complement(6494..6513)
                     /Sequence="AATCCTCCCCCTTGCTGTCC"
                     /Transferred_From="438_bGH_QPCRR2"
                     /Transferred_Similarity="100.00%"
                     /label="438_bGH_QPCRR2"
     primer_bind     complement(6499..6517)
                     /Sequence="TCCCAATCCTCCCCCTTGC"
                     /Transferred_From="439_bGH_QPCRR3"
                     /Transferred_Similarity="100.00%"
                     /label="439_bGH_QPCRR3"
     primer_bind     complement(6519..6538)
                     /Sequence="CCAGCATGCCTGCTATTGTC"
                     /Transferred_From="437_bGH_QPCRR1"
                     /Transferred_Similarity="100.00%"
                     /label="437_bGH_QPCRR1"
     primer_bind     complement(6539..6558)
                     /Sequence="CCATAGAGCCCACCGCATCC"
                     /%GC="65.0"
                     /Transferred_From="920_bGH-BamHI-rev"
                     /Transferred_Similarity="100.00%"
                     /label="920_bGH-BamHI-rev"
     primer_bind     6617..6643
                     /Sequence="GGCGTAATCATGGTCATAGCTGTTTCC"
                     /Transferred_From="654_CAG_fwd"
                     /Transferred_Similarity="100.00%"
                     /label="654_CAG_fwd"
     primer_bind     6625..6643
                     /Sequence="CATGGTCATAGCTGTTTCC"
                     /%GC="47.4"
                     /Transferred_From="NMG-1452"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1452"
     primer_bind     complement(6629..6645)
                     /created_by="primer3"
                     /annotation_group="135473983514354300085f78ef4-0452-4fae-a
                     bba-93b35302c171"
                     /%GC="47.1"
                     /Tm="49.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CAGGAAACAGCTATGAC"
                     /Transferred_From="M13-R (-26)"
                     /Transferred_Similarity="100.00%"
                     /label="M13-R (-26)"
     primer_bind     complement(6629..6645)
                     /Sequence="CAGGAAACAGCTATGAC"
                     /Transferred_From="Eton M13R Reverse"
                     /Transferred_Similarity="100.00%"
                     /label="Eton M13R Reverse"
     primer_bind     complement(6629..6645)
                     /Sequence="CAGGAAACAGCTATGAC"
                     /Transferred_From="QB0048 M13 Reverse"
                     /Transferred_Similarity="100.00%"
                     /label="QB0048 M13 Reverse"
     primer_bind     complement(6629..6645)
                     /Sequence="CAGGAAACAGCTATGAC"
                     /Transferred_From="Genewiz M13R"
                     /Transferred_Similarity="100.00%"
                     /label="Genewiz M13R"
     primer_bind     complement(6642..6665)
                     /created_by="primer3"
                     /annotation_group="1354739835389783000b8ada402-17c3-46ef-9
                     adc-177f91dab407"
                     /%GC="45.8"
                     /Tm="60.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GAGCGGATAACAATTTCACACAGG"
                     /Transferred_From="M13-R (-46)"
                     /Transferred_Similarity="100.00%"
                     /label="M13-R (-46)"
     primer_bind     complement(6642..6665)
                     /Sequence="GAGCGGATAACAATTTCACACAGG"
                     /Transferred_From="QB0108 Tac promoter"
                     /Transferred_Similarity="100.00%"
                     /label="QB0108 Tac promoter"
     primer_bind     complement(6642..6664)
                     /Sequence="AGCGGATAACAATTTCACACAGG"
                     /Transferred_From="QB0050 M13/pUC Reverse"
                     /Transferred_Similarity="100.00%"
                     /label="QB0050 M13/pUC Reverse"
     Promoter        complement(6678..6707)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lac prom"
                     /Transferred_Similarity="100.00%"
                     /label="lac prom"
     primer_bind     6704..6734
                     /Sequence="TAAAGCCTAGGGTGCCTAATGAGTGAGCTAA"
                     /%GC="45.2"
                     /Transferred_From="NMG-850"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-850"
     primer_bind     complement(6714..6733)
                     /Sequence="TAGCTCACTCATTAGGCACC"
                     /Transferred_From="Lac-F (EtonSeq)"
                     /Transferred_Similarity="100.00%"
                     /label="Lac-F (EtonSeq)"
     primer_bind     complement(6714..6733)
                     /Sequence="TAGCTCACTCATTAGGCACC"
                     /%GC="50.0"
                     /Transferred_From="836_Lac-F"
                     /Transferred_Similarity="100.00%"
                     /label="836_Lac-F"
     misc_feature    complement(6722..6746)
                     /Sequence="AATTAATGTGAGTTAGCTCACTCAT"
                     /Hairpin_Tm="58.9"
                     /Tm="56.6"
                     /Self_Dimer_Tm="15.0"
                     /%GC="32.0"
                     /created_by="primer3"
                     /label="ACK32"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     6722..6746
                     /Sequence="ATGAGTGAGCTAACTCACATTAATT"
                     /Hairpin_Tm="63.4"
                     /Tm="56.6"
                     /Self_Dimer_Tm="30.8"
                     /%GC="32.0"
                     /created_by="primer3"
                     /label="ACK21"
     primer_bind     complement(6722..6746)
                     /Sequence="AATTAATGTGAGTTAGCTCACTCAT"
                     /%GC="32.0"
                     /Transferred_From="ACK-32"
                     /Transferred_Similarity="100.00%"
                     /label="ACK-32"
     primer_bind     6780..6806
                     /Sequence="AACCTGTCGTGCCAGCTGCATTAATGA"
                     /%GC="48.1"
                     /Transferred_From="NMG-1101"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1101"
     primer_bind     complement(6832..6852)
                     /Sequence="AAGAGCGCCCAAUACGCAAAC"
                     /%GC="52.4"
                     /Transferred_From="PC0187"
                     /Transferred_Similarity="100.00%"
                     /label="PC0187"
     primer_bind     complement(6860..6877)
                     /Sequence="AGCGAGTCAGTGAGCGAG"
                     /Transferred_From="QB0039 L4440"
                     /Transferred_Similarity="100.00%"
                     /label="QB0039 L4440"
     primer_bind     complement(6912..6931)
                     /created_by="primer3"
                     /annotation_group="13547385713896020002e37130e-0600-4795-b
                     4cf-db36738ae7a4"
                     /%GC="50.0"
                     /Tm="58.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATTACCGCCTTTGAGTGAGC"
                     /modified_by="User"
                     /Transferred_From="VR"
                     /Transferred_Similarity="100.00%"
                     /label="VR"
     primer_bind     complement(7001..7022)
                     /Sequence="CAACGCGGCCTTTTTACGGTTC"
                     /Transferred_From="11_pFYF_Seq_Rev"
                     /Transferred_Similarity="100.00%"
                     /label="11_pFYF_Seq_Rev"
     misc_feature    complement(7016..7635)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.52%"
                     /label="pBR322 origin"
                     /note="Geneious type: Origin of Replication"
     primer_bind     7031..7055
                     /Sequence="TTTCCATAGGCTCCGCCCCCCTGAC"
                     /Hairpin_Tm="52.7"
                     /Tm="69.9"
                     /Self_Dimer_Tm="None"
                     /%GC="64.0"
                     /created_by="primer3"
                     /label="ACK10"
     primer_bind     complement(7092..7111)
                     /Sequence="TATAGTCCTGTCGGGTTTCG"
                     /Transferred_From="BR322-F"
                     /Transferred_Similarity="100.00%"
                     /label="BR322-F"
     misc_feature    7227
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     7239..7266
                     /Sequence="AGGTATCTCAGTTCGGTGTAGGTCGTTC"
                     /%GC="50.0"
                     /Transferred_From="NMG-851"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-851"
     primer_bind     7254..7272
                     /Sequence="GTGTAGGTCGTTCGCTCCA"
                     /Transferred_From="QB0766 RepO-F"
                     /Transferred_Similarity="100.00%"
                     /label="QB0766 RepO-F"
     primer_bind     complement(7329..7355)
                     /Sequence="TACCGGGTTGGACTCAAGACGATAGTT"
                     /%GC="48.1"
                     /Transferred_From="NMG-309"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-309"
     primer_bind     7351..7370
                     /Sequence="CGGTAAGACACGACTTATCG"
                     /%GC="50.0"
                     /Transferred_From="833_BR322-rev1"
                     /Transferred_Similarity="100.00%"
                     /label="833_BR322-rev1"
     primer_bind     complement(7379..7398)
                     /Sequence="TCCTGTTACCAGTGGCTGCT"
                     /Transferred_From="QB0767 RepO-R"
                     /Transferred_Similarity="100.00%"
                     /label="QB0767 RepO-R"
     misc_feature    7472
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     7532..7556
                     /Sequence="CTCTTGATCCGGCAAACAAACCACC"
                     /Hairpin_Tm="None"
                     /Tm="64.7"
                     /Self_Dimer_Tm="2.9"
                     /%GC="52.0"
                     /created_by="primer3"
                     /label="ACK11"
     primer_bind     complement(7532..7551)
                     /Sequence="TTTGTTTGCCGGATCAAGAG"
                     /%GC="45.0"
                     /Transferred_From="832_BR322-fwd1"
                     /Transferred_Similarity="100.00%"
                     /label="832_BR322-fwd1"
     primer_bind     7782..7810
                     /Sequence="TCTGACAGTTACCAATGCTTAATCAGTGA"
                     /%GC="37.9"
                     /Transferred_From="NMG-853"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-853"
     Marker          complement(7790..8650)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp marker"
                     /Transferred_Similarity="99.88%"
                     /label="amp marker"
     primer_bind     7940..7969
                     /Sequence="CTCACCGGCTCCAGATTTATCAGCAATAAA"
                     /%GC="43.3"
                     /Transferred_From="NMG-584"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-584"
     primer_bind     8031..8055
                     /Sequence="TCTATTAATTGTTGCCGGGAAGCTA"
                     /Hairpin_Tm="None"
                     /Tm="60.4"
                     /Self_Dimer_Tm="9.1"
                     /%GC="40.0"
                     /created_by="primer3"
                     /label="ACK12"
     primer_bind     8139..8165
                     /Sequence="ATGGCTTCATTCAGCTCCGGTTCCCAA"
                     /%GC="51.9"
                     /Transferred_From="NMG-1102"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1102"
     primer_bind     8196..8215
                     /Sequence="TTGTGCAAAAAAGCGGTTAG"
                     /Transferred_From="ACK563"
                     /Transferred_Similarity="100.00%"
                     /label="ACK563"
     primer_bind     8314..8342
                     /Sequence="CCGTAAGATGCTTTTCTGTGACTGGTGAG"
                     /%GC="48.3"
                     /Transferred_From="NMG-854"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-854"
     primer_bind     8413..8432
                     /Sequence="ATAATACCGCGCCACATAGC"
                     /Transferred_From="QB0005 Amp-R"
                     /Transferred_Similarity="100.00%"
                     /label="QB0005 Amp-R"
     primer_bind     8474..8503
                     /Sequence="GCGAAAACTCTCAAGGATCTTACCGCTGTT"
                     /%GC="46.7"
                     /Transferred_From="NMG-585"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-585"
     primer_bind     8498..8525
                     /Sequence="GCTGTTGAGATCCAGTTCGATGTAACCC"
                     /Transferred_From="563_Amp_Rev"
                     /Transferred_Similarity="100.00%"
                     /label="563_Amp_Rev"
     primer_bind     complement(8498..8525)
                     /Sequence="GGGTTACATCGAACTGGATCTCAACAGC"
                     /Transferred_From="562_Amp_Fwd"
                     /Transferred_Similarity="100.00%"
                     /label="562_Amp_Fwd"
     primer_bind     8531..8555
                     /Sequence="TGCACCCAACTGATCTTCAGCATCT"
                     /Hairpin_Tm="50.2"
                     /Tm="64.5"
                     /Self_Dimer_Tm="None"
                     /%GC="48.0"
                     /created_by="primer3"
                     /label="ACK13"
     primer_bind     8632..8661
                     /Sequence="GGAAATGTTGAATACTCATACTCTTCCTTT"
                     /%GC="33.3"
                     /Transferred_From="NMG-396"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-396"
     primer_bind     8689..8711
                     /Sequence="GTTATTGTCTCATGAGCGGATAC"
                     /%GC="43.5"
                     /Transferred_From="NMG-1469"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1469"
     Promoter        complement(8692..8720)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="100.00%"
                     /label="amp prom"
     primer_bind     8705..8731
                     /Sequence="CGGATACATATTTGAATGTATTTAGAA"
                     /%GC="25.9"
                     /Transferred_From="NMG-1103"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1103"
     primer_bind     8764..8781
                     /Sequence="CCCGAAAAGTGCCACCTG"
                     /Transferred_From="QB0090 pQE promoter"
                     /Transferred_Similarity="100.00%"
                     /label="QB0090 pQE promoter"
     primer_bind     8771..8799
                     /Sequence="AGTGCCACCTGACGTCGACGGATCGGGAG"
                     /%GC="65.5"
                     /Transferred_From="NMG-838"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-838"
     primer_bind     complement(8834..8853)
                     /Sequence="CGGCATCAGAGCAGATTGTA"
                     /Transferred_From="QB0092 pRS-marker"
                     /Transferred_Similarity="100.00%"
                     /label="QB0092 pRS-marker"
     primer_bind     8999..9019
                     /Sequence="CGATGTACGGGCCAGATATAC"
                     /%GC="52.4"
                     /Transferred_From="PC0037"
                     /Transferred_Similarity="100.00%"
                     /label="PC0037"
     primer_bind     9034..9058
                     /Sequence="TATTGACTAGTTATTAATAGTAATC"
                     /Hairpin_Tm="32.1"
                     /Tm="48.0"
                     /Self_Dimer_Tm="5.9"
                     /%GC="20.0"
                     /created_by="primer3"
                     /label="ACK14"
     primer_bind     9034..9058
                     /Sequence="TATTGACTAGTTATTAATAGTAATC"
                     /Transferred_From="JLseq006_ACK14"
                     /Transferred_Similarity="100.00%"
                     /label="JLseq006_ACK14"
     promoter        9041..356
                     /created_by="User"
                     /modified_by="User"
                     /Transferred_From="CMV_Full"
                     /Transferred_Similarity="99.83%"
                     /label="CMV_Full"
     primer_bind     9041..9084
                     /Sequence="TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCC"
                     /Transferred_From="387_pAAV_fwd"
                     /Transferred_Similarity="100.00%"
                     /label="387_pAAV_fwd"
     primer_bind     complement(9091..9110)
                     /Sequence="TTATGTAACGCGGAACTCCA"
                     /Transferred_From="QB1110 pSicoR"
                     /Transferred_Similarity="100.00%"
                     /label="QB1110 pSicoR"
     misc_feature    9230
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
ORIGIN      
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcgggaa gcgaaattac
      481 attctggggc tggacattgg cattacatca gtgggctatg gcatcattga ctacgagaca
      541 agggacgtga tcgacgccgg cgtgagactg ttcaaggagg ccaacgtgga gaacaatgag
      601 ggccggagat ccaagagggg agcaaggcgc ctgaagcgga gaaggcgcca cagaatccag
      661 agagtgaaga agctgctgtt cgattacaac ctgctgaccg accactccga gctgtctggc
      721 atcaatcctt atgaggccag agtgaagggc ctgtcccaga agctgtctga ggaggagttt
      781 agcgccgccc tgctgcacct ggcaaagagg agaggcgtgc acaacgtgaa tgaggtggag
      841 gaggacaccg gcaacgagct gtccacaaag gagcagatca gccgcaattc caaggccctg
      901 gaggagaagt atgtggccga gctgcagctg gagcggctga agaaggatgg cgaggtgagg
      961 ggctccatca atcgcttcaa gacctctgac tacgtgaagg aggccaagca gctgctgaag
     1021 gtgcagaagg cctaccacca gctggatcag tcctttatcg atacatatat cgacctgctg
     1081 gagacaaggc gcacatacta tgagggacca ggagagggct ctcccttcgg ctggaaggac
     1141 atcaaggagt ggtacgagat gctgatgggc cactgcacct attttccaga ggagctgaga
     1201 agcgtgaagt acgcctataa cgccgatctg tacaacgccc tgaatgacct gaacaacctg
     1261 gtcatcacca gggatgagaa cgagaagctg gagtactatg agaagttcca gatcatcgag
     1321 aacgtgttca agcagaagaa gaagcctaca ctgaagcaga tcgccaagga gatcctggtg
     1381 aacgaggagg acatcaaggg ctaccgcgtg acctccacag gcaagccaga gttcaccaat
     1441 ctgaaggtgt atcacgatat caaggacatc acagcccgga aggagatcat cgagaacgcc
     1501 gagctgctgg atcagatcgc caagatcctg accatctatc agagctccga ggacatccag
     1561 gaggagctga ccaacctgaa tagcgagctg acacaggagg agatcgagca gatcagcaat
     1621 ctgaagggct acaccggcac acacaacctg agcctgaagg ccatcaatct gatcctggat
     1681 gagctgtggc acacaaacga caatcagatc gccatcttta accggctgaa gctggtgcca
     1741 aagaaggtgg acctgtccca gcagaaggag atcccaacca cactggtgga cgatttcatc
     1801 ctgtctcccg tggtgaagcg gagcttcatc cagagcatca aagtgatcaa cgccatcatc
     1861 aagaagtacg gcctgcccaa tgatatcatc atcgagctgg ccagggagaa gaactccaag
     1921 gacgcccaga agatgatcaa tgagatgcag aagaggaacc gccagaccaa tgagcggatc
     1981 gaggagatca tcagaaccac aggcaaggag aacgccaagt acctgatcga gaagatcaag
     2041 ctgcacgata tgcaggaggg caagtgtctg tattctctgg aggccatccc tctggaggac
     2101 ctgctgaaca atccattcaa ctacgaggtg gatcacatca tcccccggag cgtgagcttc
     2161 gacaattctt ttaacaataa ggtgctggtg aagcaggagg aggccagcaa gaagggcaat
     2221 aggacccctt tccagtacct gtctagctcc gattctaaga tcagctacga gacattcaag
     2281 aagcacatcc tgaatctggc caagggcaag ggccgcatca gcaagaccaa gaaggagtac
     2341 ctgctggagg agcgggacat caacagattc tccgtgcaga aggacttcat caaccggaat
     2401 ctggtggaca ccagatacgc cacacgcggc ctgatgaatc tgctgcggtc ttatttcaga
     2461 gtgaacaatc tggatgtgaa ggtgaagagc atcaacggcg gcttcacctc ctttctgcgg
     2521 agaaagtgga agtttaagaa ggagcgcaac aagggctata agcaccacgc cgaggatgcc
     2581 ctgatcatcg ccaatgccga cttcatcttt aaggagtgga agaagctgga caaggccaag
     2641 aaagtgatgg agaaccagat gttcgaggag aagcaggccg agagcatgcc cgagatcgag
     2701 acagagcagg agtacaagga gattttcatc acacctcacc agatcaagca catcaaggac
     2761 ttcaaggact acaagtattc tcacagggtg gataagaagc ccaaccgcga gctgatcaat
     2821 gacaccctgt atagcacacg gaaggacgat aagggcaata ccctgatcgt gaacaatctg
     2881 aacggcctgt acgacaagga taatgacaag ctgaagaagc tgatcaacaa gtctcccgag
     2941 aagctgctga tgtaccacca cgatcctcag acatatcaga agctgaagct gatcatggag
     3001 cagtacggcg acgagaagaa cccactgtat aagtactatg aggagacagg caactacctg
     3061 acaaagtata gcaagaagga taatggcccc gtgatcaaga agatcaagta ctatggcaac
     3121 aagctgaatg cccacctgga catcaccgac gattacccta actctcgcaa taaggtggtg
     3181 aagctgagcc tgaagccata ccggttcgac gtgtacctgg acaacggcgt gtataagttt
     3241 gtgacagtga agaatctgga tgtgatcaag aaggagaact actatgaggt gaacagcaag
     3301 tgctacgagg aggccaagaa gctgaagaag atcagcaacc aggccgagtt catcgcctct
     3361 ttttacaaca atgacctgat caagatcaat ggcgagctgt atagagtgat cggcgtgaac
     3421 aatgatctgc tgaacagaat cgaagtgaat atgatcgaca tcacctacag ggagtatctg
     3481 gagaacatga atgataagag gccccctcgc atcatcaaga ccatcgcctc taagacacag
     3541 agcatcaaga agtacagcac agacatcctg gggaacctgt atgaagtcaa gagcaagaaa
     3601 catcctcaga ttatcaagaa aggctctgga ggatctagcg gaggatcctc tggcagcgag
     3661 acaccaggaa caagcgagtc agcaacacca gagagcagtg gcggcagcag cggcggcagc
     3721 agcaccctaa atatagaaga tgagtatcgg ctacatgaga cctcaaaaga gccagatgtt
     3781 tctctagggt ccacatggct gtctgatttt cctcaggcct gggcggaaac cgggggcatg
     3841 ggactggcag ttcgccaagc tcctctgatc atacctctga aagcaacctc tacccccgtg
     3901 tccataaaac aataccccat gtcacaagaa gccagactgg ggatcaagcc ccacatacag
     3961 agactgttgg accagggaat actggtaccc tgccagtccc cctggaacac gcccctgcta
     4021 cccgttaaga aaccagggac taatgattat aggcctgtcc aggatctgag agaagtcaac
     4081 aagcgggtgg aagacatcca ccccaccgtg cccaaccctt acaacctctt gagcgggctc
     4141 ccaccgtccc accagtggta cactgtgctt gatttaaagg atgccttttt ctgcctgaga
     4201 ctccacccca ccagtcagcc tctcttcgcc tttgagtgga gagatccaga gatgggaatc
     4261 tcaggacaat tgacctggac cagactccca cagggtttca aaaacagtcc caccctgttt
     4321 aatgaggcac tgcacagaga cctagcagac ttccggatcc agcacccaga cttgatcctg
     4381 ctacagtacg tggatgactt actgctggcc gccacttctg agctagactg ccaacaaggt
     4441 actcgggccc tgttacaaac cctagggaac ctcgggtatc gggcctcggc caagaaagcc
     4501 caaatttgcc agaaacaggt caagtatctg gggtatcttc taaaagaggg tcagagatgg
     4561 ctgactgagg ccagaaaaga gactgtgatg gggcagccta ctccgaagac ccctcgacaa
     4621 ctaagggagt tcctagggaa ggcaggcttc tgtcgcctct tcatccctgg gtttgcagaa
     4681 atggcagccc ccctgtaccc tctcaccaaa ccggggactc tgtttaattg gggcccagac
     4741 caacaaaagg cctatcaaga aatcaagcaa gctcttctaa ctgccccagc cctggggttg
     4801 ccagatttga ctaagccctt tgaactcttt gtcgacgaga agcagggcta cgccaaaggt
     4861 gtcctaacgc aaaaactggg accttggcgt cggccggtgg cctacctgtc caaaaagcta
     4921 gacccagtag cagctgggtg gcccccttgc ctacggatgg tagcagccat tgccgtactg
     4981 acaaaggatg caggcaagct aaccatggga cagccactag tcattctggc cccccatgca
     5041 gtagaggcac tagtcaaaca accccccgac cgctggcttt ccaacgcccg gatgactcac
     5101 tatcaggcct tgcttttgga cacggaccgg gtccagttcg gaccggtggt agccctgaac
     5161 ccggctacgc tgctcccact gcctgaggaa gggctgcaac acaactgcct tgatatcctg
     5221 gccgaagccc acggaacccg acccgaccta acggaccagc cgctcccaga cgccgaccac
     5281 acctggtaca cggatggaag cagtctctta caagagggac agcgtaaggc gggagctgcg
     5341 gtgaccaccg agaccgaggt aatctgggct aaagccctgc cagccgggac atccgctcag
     5401 cgggctgaac tgatagcact cacccaggcc ctaaagatgg cagaaggtaa gaagctaaat
     5461 gtttatactg atagccgtta tgcttttgct actgcccata tccatggaga aatatacaga
     5521 aggcgtgggt ggctcacatc agaaggcaaa gagatcaaaa ataaagacga gatcttggcc
     5581 ctactaaaag ccctctttct gcccaaaaga cttagcataa tccattgtcc aggacatcaa
     5641 aagggacaca gcgccgaggc tagaggcaac cggatggctg accaagcggc ccgaaaggca
     5701 gccatcacag agactccaga cacctctacc ctcctcatag aaaattcatc accctctggc
     5761 ggctcaaaaa gaaccgccga cggcagcgaa ttcgagccca agaagaagag gaaagtcgga
     5821 tccggcgcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga
     5881 ccgatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca
     5941 atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac
     6001 ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc
     6061 gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc
     6121 ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat
     6181 ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg
     6241 attcgtgaat tgctgccctc tggttatgtg tgggagggct aaccggtcat catcaccatc
     6301 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     6361 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     6421 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     6481 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     6541 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga
     6601 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6661 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct
     6721 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6781 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6841 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6901 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6961 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7021 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7081 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7141 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7201 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7261 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7321 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7381 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7441 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7501 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7561 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7621 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7681 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7741 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7801 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7861 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7921 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7981 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8041 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8101 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8161 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8221 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8281 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8341 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8401 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8461 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8521 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8581 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8641 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8701 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8761 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag
     8821 ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     8881 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     8941 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     9001 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     9061 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     9121 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     9181 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     9241 aaactgccca cttggcagta catcaagtgt atc
//
