LOCUS       pPC1301                 6302 bp ds-DNA     circular     21-JUL-2021
DEFINITION  [pBR322, Cb] pEF1a-hMLH1 del754-756.
ACCESSION   urn.local...4v-c5kndyu
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     primer_bind     1..21
                     /Sequence="CGATGTACGGGCCAGATATAC"
                     /%GC="52.4"
                     /Transferred_From="PC0037"
                     /Transferred_Similarity="100.00%"
                     /label="PC0037"
     promoter        43..1300
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="EF-1a"
     misc_feature    43..72
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /label="GH"
     misc_feature    46..60
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /label="USER"
     promoter        142..353
                     /%_Identity="99.52830188679245"
                     /Motif="GGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTC
                     GGCAATTGATCCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCG
                     TGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGT
                     CGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAG"
                     /annotation_group="GGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGG
                     GGGGAGGGGTCGGCAATTGATCCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGA
                     AAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATA
                     AGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAG:
                     142 -> 353"
                     /modified_by="chenp"
                     /label="EFS (with mismatch)"
     mismatch        201
                     /Motif="GGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTC
                     GGCAATTGATCCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCG
                     TGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGT
                     CGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAG"
                     /annotation_group="GGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGG
                     GGGGAGGGGTCGGCAATTGATCCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGA
                     AAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATA
                     AGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAG:
                     142 -> 353"
                     /label="T"
     mutation        complement(429..430)
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="G insertion"
     primer_bind     complement(610..638)
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /label="PC0043"
     primer_bind     684..703
                     /Sequence="GCCAAGATCTGCACACTGGT"
                     /Tm="60.6"
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="10.0"
                     /created_by="chenp"
                     /label="BP0033"
     primer_bind     1213..1234
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="BP0132 (EF1a-For)"
     misc_feature    1304..1315
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /label="USER"
     primer_bind     complement(1306..1335)
                     /Sequence="ACGAACGACATGGTGGCTCTAGAGGATCCC"
                     /Tm="70.4"
                     /%GC="56.7"
                     /Hairpin_Tm="33.7"
                     /Self_Dimer_Tm="21.2"
                     /created_by="chenp"
                     /label="PC1165"
     regulatory      1319..1324
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /modified_by="chenp"
                     /label="Kozak"
     USER            1322..1335
                     /created_by="chenp"
     primer_bind     1322..1351
                     /created_by="chenp"
                     /Sequence="ACCATGTCGTTCGTGGCAGGGGTTATTCGG"
                     /Tm="71.7"
                     /%GC="56.7"
                     /Hairpin_Tm="52.3"
                     /Self_Dimer_Tm="16.9"
                     /modified_by="chenp"
                     /label="PC1164"
     CDS             1325..>3583
                     /created_by="chenp"
                     /modified_by="chenp"
                     /label="MLH1 delC754-756"
     misc_feature    1328..3582
                     /Original_Bases="GCGAGTAGCGAAGACGTTATCAAAGAGTTCATGCGTTTCAA
                     AGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATCGAAGGTGAAGGTGAA
                     GGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACCAAAGGTGGTCCGC
                     TGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCCAAAGCTTACGT
                     TAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAAGGTTTCAAA
                     TGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAGGACTCCT
                     CCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTCCCGTC
                     CGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGTATG
                     TACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGACG
                     GTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCT
                     GCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTAC
                     ACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACAGGTGC"
                     /label="GCGAGTAGCGAAGACGTTATCAAAGAGTTCATGCGTTTCAAAGTTCGTAT
                     GGAAGGTTCCGTTAACGGTCACGAGTTCGAAATCGAAGGTGAAGGTGAAGGTCGTCCG
                     TACGAAGGTACCCAGACCGCTAAACTGAAAGTTACCAAAGGTGGTCCGCTGCCGTTCG
                     CTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCCAAAGCTTACGTTAAACACCC
                     GGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAAGGTTTCAAATGGGAACGT
                     GTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAGGACTCCTCCCTGCAAG
                     ACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTCCCGTCCGACGGTCC
                     GGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGTATGTACCCGGAA
                     GACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGACGGTGGTCACT
                     ACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTGCCGGGTGC
                     TTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACCATCGTT
                     GAACAGTACGAACGTGCTGAAGGTCGTCACTCCACAGGTGC"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     1509..1529
                     /Sequence="AAGACAATGGCACCGGGATCA"
                     /Tm="62.1"
                     /%GC="52.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="6.3"
                     /created_by="chenp"
                     /label="PC1233"
     primer_bind     complement(1614..1634)
                     /Sequence="AAGCCTCACCTCGAAAGCCAT"
                     /Tm="62.1"
                     /%GC="52.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1234"
     primer_bind     1614..1634
                     /Sequence="ATGGCTTTCGAGGTGAGGCTT"
                     /Tm="62.1"
                     /%GC="52.4"
                     /Hairpin_Tm="37.0"
                     /Self_Dimer_Tm="12.5"
                     /created_by="chenp"
                     /label="PC1235"
     primer_bind     complement(1752..1772)
                     /Sequence="TCTGGGTCCCTTGATTGCCAG"
                     /Tm="62.1"
                     /%GC="57.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="10.0"
                     /created_by="chenp"
                     /label="PC1236"
     primer_bind     1916..1939
                     /Sequence="GGAGAGACAGTAGCTGATGTTAGG"
                     /Tm="60.0"
                     /%GC="50.0"
                     /Hairpin_Tm="35.2"
                     /Self_Dimer_Tm="7.0"
                     /created_by="chenp"
                     /label="PC1203"
     primer_bind     2602..2624
                     /Sequence="GCAAGATGAGGAGATGCTTGAAC"
                     /Tm="59.9"
                     /%GC="47.8"
                     /Hairpin_Tm="38.5"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1204"
     primer_bind     complement(3555..3591)
                     /Sequence="ACCGGTTAAAAGACTTTGTATAGATCAGGCAGGTTAG"
                     /Tm="61.1"
                     /%GC="40.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1316"
     USER            3577..3591
                     /created_by="chenp"
     primer_bind     3577..3613
                     /Sequence="AGTCTTTTAACCGGTCATCATCACCATCACCATTGAG"
                     /Tm="62.9"
                     /%GC="43.2"
                     /Hairpin_Tm="29.8"
                     /Self_Dimer_Tm="4.2"
                     /created_by="chenp"
                     /label="PC1315"
     Terminator      3635..3862
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="bGH_PA term"
     misc_feature    complement(3784..3808)
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /label="ACK64"
     primer_bind     complement(3784..3808)
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="ACK64 (end)"
     primer_bind     complement(3798..3817)
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /label="438_bGH_QPCRR2"
     primer_bind     complement(3803..3821)
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="439_bGH_QPCRR3"
     primer_bind     complement(3823..3842)
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="437_bGH_QPCRR1"
     primer_bind     complement(3843..3862)
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="920_bGH-BamHI-rev"
     primer_bind     3921..3947
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /label="654_CAG_fwd"
     primer_bind     3929..3947
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="NMG-1452"
     primer_bind     complement(3933..3949)
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="M13-R (-26)"
     primer_bind     complement(3933..3949)
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="QB0048 M13 Reverse"
     primer_bind     complement(3933..3949)
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /label="Genewiz M13R"
     primer_bind     complement(3933..3949)
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /label="Eton M13R Reverse"
     primer_bind     complement(3946..3969)
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /label="M13-R (-46)"
     primer_bind     complement(3946..3969)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /label="QB0108 Tac promoter"
     primer_bind     complement(3946..3968)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /label="QB0050 M13/pUC Reverse"
     Promoter        complement(3982..4011)
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /label="lac prom"
     primer_bind     4008..4038
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /label="NMG-850"
     primer_bind     complement(4018..4037)
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /label="Lac-F (EtonSeq)"
     primer_bind     complement(4018..4037)
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="836_Lac-F"
     misc_feature    complement(4026..4050)
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="ACK32"
     primer_bind     4026..4050
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="ACK21"
     primer_bind     complement(4026..4050)
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="ACK-32"
     primer_bind     4084..4110
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /label="NMG-1101"
     primer_bind     complement(4164..4181)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /label="QB0039 L4440"
     primer_bind     complement(4216..4235)
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="VR"
     primer_bind     complement(4305..4326)
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /label="11_pFYF_Seq_Rev"
     misc_feature    complement(4320..4939)
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /label="pBR322 origin"
     primer_bind     4335..4359
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /label="ACK10"
     primer_bind     complement(4396..4415)
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /label="BR322-F"
     misc_feature    4531
                     /label="/ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9"
     primer_bind     4543..4570
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /label="NMG-851"
     primer_bind     4558..4576
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /label="QB0766 RepO-F"
     primer_bind     complement(4633..4659)
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="NMG-309"
     primer_bind     4655..4674
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="833_BR322-rev1"
     primer_bind     complement(4683..4702)
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /label="QB0767 RepO-R"
     misc_feature    4776
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="G"
     primer_bind     4836..4860
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="ACK11"
     primer_bind     complement(4836..4855)
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="832_BR322-fwd1"
     primer_bind     5086..5114
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /label="NMG-853"
     Marker          complement(5094..5954)
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="amp marker"
     primer_bind     5244..5273
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /label="NMG-584"
     primer_bind     5335..5359
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="ACK12"
     primer_bind     5443..5469
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="NMG-1102"
     primer_bind     5500..5519
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="ACK563"
     primer_bind     5618..5646
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /label="NMG-854"
     primer_bind     5717..5736
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="QB0005 Amp-R"
     primer_bind     5778..5807
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="NMG-585"
     primer_bind     complement(5802..5829)
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="562_Amp_Fwd"
     primer_bind     5802..5829
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="563_Amp_Rev"
     primer_bind     5835..5859
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /label="ACK13"
     primer_bind     5936..5965
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /label="NMG-396"
     primer_bind     5993..6015
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="NMG-1469"
     Promoter        complement(5996..6024)
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /label="amp prom"
     primer_bind     6009..6035
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="NMG-1103"
     primer_bind     6068..6085
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="QB0090 pQE promoter"
     primer_bind     6075..6103
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="NMG-838"
     primer_bind     complement(6138..6157)
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /label="QB0092 pRS-marker"
ORIGIN      
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actgcaaaga tggataaagt
       61 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa
      121 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
      181 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
      241 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
      301 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
      361 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
      421 ttacttccac tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
      481 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
      541 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
      601 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
      661 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
      721 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
      781 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
      841 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
      901 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
      961 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     1021 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     1081 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     1141 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     1201 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     1261 agtggttcaa agtttttttc ttccatttca ggtgtcgtga cgtacgggat cctctagagc
     1321 caccatgtcg ttcgtggcag gggttattcg gcggctggac gagacagtgg tgaaccgcat
     1381 cgcggcgggg gaagttatcc agcggccagc taatgctatc aaagagatga ttgagaactg
     1441 tttagatgca aaatccacaa gtattcaagt gattgttaaa gagggaggcc tgaagttgat
     1501 tcagatccaa gacaatggca ccgggatcag gaaagaagat ctggatattg tatgtgaaag
     1561 gttcactact agtaaactgc agtcctttga ggatttagcc agtatttcta cctatggctt
     1621 tcgaggtgag gctttggcca gcataagcca tgtggctcat gttactatta caacgaaaac
     1681 agctgatgga aagtgtgcat acagagcaag ttactcagat ggaaaactga aagcccctcc
     1741 taaaccatgt gctggcaatc aagggaccca gatcacggtg gaggaccttt tttacaacat
     1801 agccacgagg agaaaagctt taaaaaatcc aagtgaagaa tatgggaaaa ttttggaagt
     1861 tgttggcagg tattcagtac acaatgcagg cattagtttc tcagttaaaa aacaaggaga
     1921 gacagtagct gatgttagga cactacccaa tgcctcaacc gtggacaata ttcgctccat
     1981 ctttggaaat gctgttagtc gagaactgat agaaattgga tgtgaggata aaaccctagc
     2041 cttcaaaatg aatggttaca tatccaatgc aaactactca gtgaagaagt gcatcttctt
     2101 actcttcatc aaccatcgtc tggtagaatc aacttccttg agaaaagcca tagaaacagt
     2161 gtatgcagcc tatttgccca aaaacacaca cccattcctg tacctcagtt tagaaatcag
     2221 tccccagaat gtggatgtta atgtgcaccc cacaaagcat gaagttcact tcctgcacga
     2281 ggagagcatc ctggagcggg tgcagcagca catcgagagc aagctcctgg gctccaattc
     2341 ctccaggatg tacttcaccc agactttgct accaggactt gctggcccct ctggggagat
     2401 ggttaaatcc acaacaagtc tgacctcgtc ttctacttct ggaagtagtg ataaggtcta
     2461 tgcccaccag atggttcgta cagattcccg ggaacagaag cttgatgcat ttctgcagcc
     2521 tctgagcaaa cccctgtcca gtcagcccca ggccattgtc acagaggata agacagatat
     2581 ttctagtggc agggctaggc agcaagatga ggagatgctt gaactcccag cccctgctga
     2641 agtggctgcc aaaaatcaga gcttggaggg ggatacaaca aaggggactt cagaaatgtc
     2701 agagaagaga ggacctactt ccagcaaccc cagaaagaga catcgggaag attctgatgt
     2761 ggaaatggtg gaagatgatt cccgaaagga aatgactgca gcttgtaccc cccggagaag
     2821 gatcattaac ctcactagtg ttttgagtct ccaggaagaa attaatgagc agggacatga
     2881 ggttctccgg gagatgttgc ataaccactc cttcgtgggc tgtgtgaatc ctcagtgggc
     2941 cttggcacag catcaaacca agttatacct tctcaacacc accaagctta gtgaagaact
     3001 gttctaccag atactcattt atgattttgc caattttggt gttctcaggt tatcggagcc
     3061 agcaccgctc tttgaccttg ccatgcttgc cttagatagt ccagagagtg gctggacaga
     3121 ggaagatggt cccaaagaag gacttgctga atacattgtt gagtttctga agaagaaggc
     3181 tgagatgctt gcagactatt tctctttgga aattgatgag gaagggaacc tgattggatt
     3241 accccttctg attgacaact atgtgccccc tttggaggga ctgcctatct tcattcttcg
     3301 actagccact gaggtgaatt gggacgaaga aaaggaatgt tttgaaagcc tcagtaaaga
     3361 atgcgctatg ttctattcca tccggaagca gtacatatct gaggagtcga ccctctcagg
     3421 ccagcagagt gaagtgcctg gctccattcc aaactcctgg aagtggactg tggaacacat
     3481 tgtctataaa gccttgcgct cacacattct gcctcctaaa catttcacag aagatggaaa
     3541 tatcctgcag cttgctaacc tgcctgatct atacaaagtc ttttaaccgg tcatcatcac
     3601 catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     3661 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3721 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3781 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     3841 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgataccg
     3901 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3961 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctagggt
     4021 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     4081 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     4141 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4201 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4261 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4321 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4381 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4441 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4501 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4561 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4621 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4681 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4741 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4801 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4861 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4921 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4981 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5041 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5101 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5161 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5221 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5281 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5341 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5401 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5461 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5521 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5581 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5641 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5701 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5761 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5821 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5881 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5941 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6001 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6061 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcgatc tcccgatccc
     6121 ctagggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     6181 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     6241 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     6301 cg
//
