LOCUS       pPC1328                 6302 bp ds-DNA     circular     23-AUG-2020
DEFINITION  [pBR322, Cb] pEF1a-hMLH1 del754-756 genscript codon.
ACCESSION   urn.local...1y-c8q7opn
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     primer_bind     1..21
                     /Sequence="CGATGTACGGGCCAGATATAC"
                     /%GC="52.4"
                     /Transferred_From="PC0037"
                     /Transferred_Similarity="100.00%"
                     /label="PC0037"
     misc_feature    complement(21..60)
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="EMJ_P0002"
     promoter        43..1300
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="EF-1a"
     misc_feature    43..72
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /label="GH"
     misc_feature    46..73
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="EMJ_P0003"
     misc_feature    46..60
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /label="USER"
     mutation        complement(429..430)
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="G insertion"
     primer_bind     complement(610..638)
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /label="PC0043"
     primer_bind     684..703
                     /Sequence="GCCAAGATCTGCACACTGGT"
                     /Tm="60.6"
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="10.0"
                     /created_by="chenp"
                     /label="BP0033"
     primer_bind     1213..1234
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="BP0132 (EF1a-For)"
     primer_bind     complement(1305..1332)
                     /Sequence="AAGCTCATGGTGGCTCTAGAGGATCCCG"
                     /Tm="69.0"
                     /%GC="57.1"
                     /Hairpin_Tm="37.9"
                     /Self_Dimer_Tm="21.2"
                     /created_by="chenp"
                     /label="PC1367"
     regulatory      1319..1324
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /modified_by="chenp"
                     /label="Kozak"
     USER            1322..1332
                     /created_by="chenp"
     primer_bind     1322..1350
                     /Sequence="ACCATGAGCTTCGTTGCTGGAGTCATCCG"
                     /Tm="70.1"
                     /%GC="55.2"
                     /Hairpin_Tm="50.1"
                     /Self_Dimer_Tm="9.4"
                     /created_by="chenp"
                     /label="PC1366"
     CDS             1325..3583
                     /created_by="chenp"
                     /modified_by="chenp"
                     /label="MLH1 del754-756 genscript codon"
     primer_bind     complement(1396..1431)
                     /Sequence="ATCATCTCCTTTATTGCATTGGCAGGTCTCTGGATC"
                     /Tm="69.0"
                     /%GC="44.4"
                     /Hairpin_Tm="53.1"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1365"
     USER            1418..1431
                     /created_by="chenp"
     primer_bind     1418..1450
                     /Sequence="ATAAAGGAGATGATCGAGAACTGTCTGGACGCC"
                     /Tm="68.7"
                     /%GC="48.5"
                     /Hairpin_Tm="40.9"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1364"
     primer_bind     1902..1924
                     /Sequence="GCGTAAAGAAGCAGGGAGAAACC"
                     /Tm="62.0"
                     /%GC="52.2"
                     /Hairpin_Tm="38.1"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1473"
     primer_bind     1903..1925
                     /Sequence="CGTAAAGAAGCAGGGAGAAACCG"
                     /Tm="61.7"
                     /%GC="52.2"
                     /Hairpin_Tm="40.1"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1418"
     primer_bind     2566..2588
                     /Sequence="GGACAAGACAGATATCTCCTCCG"
                     /Tm="59.7"
                     /%GC="52.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="1.0"
                     /created_by="chenp"
                     /label="PC1419"
     primer_bind     3285..3305
                     /Sequence="CCATCTTCATCCTGAGACTGG"
                     /Tm="57.5"
                     /%GC="52.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1472"
     primer_bind     complement(3556..3591)
                     /Sequence="ACCGGTTAGAAAACCTTATAAAGGTCGGGCAGGTTG"
                     /Tm="70.6"
                     /%GC="47.2"
                     /Hairpin_Tm="61.0"
                     /Self_Dimer_Tm="23.6"
                     /created_by="chenp"
                     /label="PC1369"
     USER            3576..3591
                     /created_by="chenp"
     primer_bind     3576..3607
                     /Sequence="AGGTTTTCTAACCGGTCATCATCACCATCACC"
                     /Tm="68.0"
                     /%GC="46.9"
                     /Hairpin_Tm="54.7"
                     /Self_Dimer_Tm="8.0"
                     /created_by="chenp"
                     /label="PC1368"
     Terminator      3635..3862
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="bGH_PA term"
     misc_feature    complement(3784..3808)
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /label="ACK64"
     primer_bind     complement(3784..3808)
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="ACK64 (end)"
     primer_bind     complement(3798..3817)
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /label="438_bGH_QPCRR2"
     primer_bind     complement(3803..3821)
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="439_bGH_QPCRR3"
     primer_bind     complement(3823..3842)
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="437_bGH_QPCRR1"
     primer_bind     complement(3843..3862)
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="920_bGH-BamHI-rev"
     primer_bind     3921..3947
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /label="654_CAG_fwd"
     primer_bind     3929..3947
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="NMG-1452"
     primer_bind     complement(3933..3949)
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="M13-R (-26)"
     primer_bind     complement(3933..3949)
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="QB0048 M13 Reverse"
     primer_bind     complement(3933..3949)
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /label="Genewiz M13R"
     primer_bind     complement(3933..3949)
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /label="Eton M13R Reverse"
     primer_bind     complement(3946..3969)
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /label="M13-R (-46)"
     primer_bind     complement(3946..3969)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /label="QB0108 Tac promoter"
     primer_bind     complement(3946..3968)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /label="QB0050 M13/pUC Reverse"
     Promoter        complement(3982..4011)
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /label="lac prom"
     primer_bind     4008..4038
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /label="NMG-850"
     primer_bind     complement(4018..4037)
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /label="Lac-F (EtonSeq)"
     primer_bind     complement(4018..4037)
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="836_Lac-F"
     misc_feature    complement(4026..4050)
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="ACK32"
     primer_bind     4026..4050
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="ACK21"
     primer_bind     complement(4026..4050)
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="ACK-32"
     primer_bind     4084..4110
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /label="NMG-1101"
     primer_bind     complement(4164..4181)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /label="QB0039 L4440"
     primer_bind     complement(4216..4235)
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="VR"
     primer_bind     complement(4305..4326)
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /label="11_pFYF_Seq_Rev"
     misc_feature    complement(4320..4939)
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /label="pBR322 origin"
     primer_bind     4335..4359
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /label="ACK10"
     primer_bind     complement(4396..4415)
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /label="BR322-F"
     misc_feature    4531
                     /label="/ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9"
     primer_bind     4543..4570
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /label="NMG-851"
     primer_bind     4558..4576
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /label="QB0766 RepO-F"
     primer_bind     complement(4620..4648)
                     /Sequence="ACTCAAGACGAUAGTTACCGGATAAGGCG"
                     /%GC="48.3"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0970"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:2e-b64q18g"">PC0970</a>"
                     /label="PC0970"
     primer_bind     complement(4633..4659)
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="NMG-309"
     primer_bind     4637..4663
                     /Sequence="ATCGTCTTGAGUCCAACCCGGTAAGAC"
                     /%GC="51.9"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0969"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-b64q18e"">PC0969</a>"
                     /label="PC0969"
     primer_bind     4655..4674
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="833_BR322-rev1"
     primer_bind     complement(4683..4702)
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /label="QB0767 RepO-R"
     misc_feature    4776
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="G"
     primer_bind     4836..4860
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="ACK11"
     primer_bind     complement(4836..4855)
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="832_BR322-fwd1"
     primer_bind     5086..5114
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /label="NMG-853"
     Marker          complement(5094..5954)
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="amp marker"
     primer_bind     5244..5273
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /label="NMG-584"
     primer_bind     5335..5359
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="ACK12"
     primer_bind     5443..5469
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="NMG-1102"
     primer_bind     5500..5519
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="ACK563"
     primer_bind     5618..5646
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /label="NMG-854"
     primer_bind     5717..5736
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="QB0005 Amp-R"
     primer_bind     5778..5807
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="NMG-585"
     primer_bind     complement(5802..5829)
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="562_Amp_Fwd"
     primer_bind     5802..5829
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="563_Amp_Rev"
     primer_bind     5835..5859
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /label="ACK13"
     primer_bind     5936..5965
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /label="NMG-396"
     primer_bind     5993..6015
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="NMG-1469"
     Promoter        complement(5996..6024)
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /label="amp prom"
     primer_bind     6009..6035
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="NMG-1103"
     primer_bind     6068..6085
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="QB0090 pQE promoter"
     primer_bind     6075..6103
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="NMG-838"
     primer_bind     complement(6138..6157)
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /label="QB0092 pRS-marker"
ORIGIN      
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actgcaaaga tggataaagt
       61 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa
      121 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
      181 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
      241 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
      301 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
      361 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
      421 ttacttccac tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
      481 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
      541 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
      601 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
      661 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
      721 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
      781 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
      841 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
      901 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
      961 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     1021 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     1081 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     1141 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     1201 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     1261 agtggttcaa agtttttttc ttccatttca ggtgtcgtga cgtacgggat cctctagagc
     1321 caccatgagc ttcgttgctg gagtcatccg gagactggac gagacagtgg tgaacagaat
     1381 tgccgccggc gaggtgatcc agagacctgc caatgcaata aaggagatga tcgagaactg
     1441 tctggacgcc aagtccacaa gcattcaggt gatcgtgaag gagggcggac tgaagctgat
     1501 ccagatccaa gacaacggca caggcatcag aaaggaagat ctggacatcg tgtgtgaacg
     1561 gttcaccaca tctaagctgc agtcttttga ggatctggcc tctatcagta cctacggctt
     1621 cagaggcgag gccctggcca gcatcagcca cgtggcccat gtgaccatca ccaccaaaac
     1681 cgccgacggc aaatgcgctt atcgcgctag ctacagcgac ggcaagctga aagccccgcc
     1741 aaagccttgc gccggcaacc agggtacaca gataacagtg gaggatctgt tctacaacat
     1801 cgccacccgg agaaaggccc tgaaaaatcc cagcgaggag tacggcaaga tcctggaagt
     1861 cgtcggcaga tactccgtgc acaacgccgg aatcagcttt agcgtaaaga agcagggaga
     1921 aaccgtggcc gatgtgcgca ccctgccaaa tgccagcacc gtggataaca tcagaagcat
     1981 tttcggaaat gccgtgtcca gagaactgat cgagatcggc tgcgaagata agaccctggc
     2041 ttttaagatg aacggctaca tcagcaacgc caattactct gtgaagaagt gcatctttct
     2101 tctgttcatc aaccacagac tggtggaaag caccagcctg cggaaagcca tcgagacagt
     2161 gtacgccgcc tacctgccta agaacaccca ccccttcctg tacctgagcc tcgagatcag
     2221 ccctcagaac gtggacgtca atgtgcatcc tacaaagcac gaggtgcact tcctgcacga
     2281 ggaaagcatc ctggaaagag tgcagcagca cattgagagc aagctgctgg gctctaacag
     2341 cagcagaatg tacttcacac agaccctgct gcctggcctg gccggcccct caggcgaaat
     2401 ggttaagtcc acaacctctc tgacctcatc cagcaccagc ggttcttccg ataaggtgta
     2461 cgcccaccag atggtgcgga ccgactctcg ggagcagaag ctggacgcct ttctgcaacc
     2521 tctgagcaaa cctctgagct ctcagcctca ggccatcgtg accgaggaca agacagatat
     2581 ctcctccggc cgtgccagac agcaggacga agaaatgctc gagctgccag ctcctgccga
     2641 ggtggccgcc aagaaccaga gcctggaggg agataccaca aagggcacca gcgaaatgag
     2701 cgagaagcgg ggccctacct ccagcaaccc cagaaaacgg caccgggagg acagcgacgt
     2761 ggaaatggtg gaggacgaca gcagaaagga aatgacagcc gcttgtaccc ctagaagaag
     2821 aatcatcaac ctgacctccg tgctgagcct gcaggaggag atcaacgagc agggccacga
     2881 ggtgctgaga gagatgctgc acaatcacag cttcgtgggc tgcgtgaacc ctcaatgggc
     2941 cctggctcag catcaaacaa agctgtacct gctgaacacc accaagctga gcgaagagct
     3001 gttctaccag atcctcatct acgacttcgc caacttcggc gtgctacgcc tgagcgagcc
     3061 cgcccctctg tttgacctgg ccatgctggc tctggatagc ccagaaagcg gctggacaga
     3121 agaggacgga cctaaagagg ggctggctga atacatcgtg gagttcctga agaaaaaggc
     3181 cgagatgctg gccgactact tttctctgga aatcgacgag gaaggcaacc tgatcggcct
     3241 gcctctgctg atcgataact acgtgcctcc cctggaaggc ctgcccatct tcatcctgag
     3301 actggctaca gaggtgaact gggacgagga aaaggaatgc ttcgagtctc tgagcaagga
     3361 gtgcgccatg ttctatagca tcagaaaaca gtacatctct gaagagagca ctctgtctgg
     3421 ccagcagagt gaagtgcccg gaagcatccc caacagctgg aagtggaccg tggaacacat
     3481 cgtgtacaag gccctgcgga gccacattct ccctcctaag cacttcaccg aggacggcaa
     3541 catcctgcag ctggccaacc tgcccgacct ttataaggtt ttctaaccgg tcatcatcac
     3601 catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     3661 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3721 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3781 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     3841 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgataccg
     3901 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3961 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctagggt
     4021 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     4081 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     4141 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4201 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4261 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4321 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4381 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4441 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4501 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4561 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4621 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4681 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4741 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4801 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4861 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4921 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4981 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5041 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5101 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5161 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5221 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5281 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5341 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5401 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5461 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5521 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5581 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5641 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5701 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5761 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5821 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5881 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5941 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6001 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6061 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcgatc tcccgatccc
     6121 ctagggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     6181 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     6241 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     6301 cg
//
