LOCUS pPC1396 6314 bp ds-DNA circular 29-SEP-2020 DEFINITION [pBR322, Cb] pEF1a-mMLH1 1-757 genscript. ACCESSION urn.local...v-cd6qx86 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers primer_bind 1..21 /Sequence="CGATGTACGGGCCAGATATAC" /%GC="52.4" /Transferred_From="PC0037" /Transferred_Similarity="100.00%" /label="PC0037" promoter 43..1300 /ApEinfo_revcolor="#d6b295" /ApEinfo_fwdcolor="#d6b295" /label="EF-1a" misc_feature 43..72 /ApEinfo_revcolor="#faac61" /ApEinfo_fwdcolor="#faac61" /label="GH" misc_feature 46..60 /ApEinfo_revcolor="#c6c9d1" /ApEinfo_fwdcolor="#c6c9d1" /label="USER" mutation complement(429..430) /ApEinfo_revcolor="#f58a5e" /ApEinfo_fwdcolor="#f58a5e" /label="G insertion" primer_bind complement(610..638) /ApEinfo_revcolor="#faac61" /ApEinfo_fwdcolor="#faac61" /label="PC0043" primer_bind 683..702 /Sequence="GGCCAAGATCTGCACACTGG" /Tm="61.3" /%GC="60.0" /Hairpin_Tm="None" /Self_Dimer_Tm="6.0" /created_by="chenp" /label="PC1506" primer_bind 684..703 /Sequence="GCCAAGATCTGCACACTGGT" /Tm="60.6" /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="10.0" /created_by="chenp" /label="BP0033" primer_bind 1213..1234 /ApEinfo_revcolor="#d6b295" /ApEinfo_fwdcolor="#d6b295" /label="BP0132 (EF1a-For)" primer_bind 1217..1239 /Sequence="GCCCTTTTTGAGTTTGGATCTTG" /Tm="58.7" /%GC="43.5" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /created_by="chenp" /label="PC1507" misc_feature 1304..1315 /ApEinfo_revcolor="#c7b0e3" /ApEinfo_fwdcolor="#c7b0e3" /label="USER" primer_bind complement(1306..1335) /Sequence="ACGAATGCCATGGTGGCTCTAGAGGATCCC" /Tm="70.7" /%GC="56.7" /Hairpin_Tm="61.4" /Self_Dimer_Tm="21.2" /created_by="chenp" /label="PC1509" regulatory 1319..1324 /ApEinfo_revcolor="#b1ff67" /ApEinfo_fwdcolor="#b1ff67" /modified_by="chenp" /label="Kozak" USER 1322..1335 /created_by="chenp" primer_bind 1322..1350 /Sequence="ACCATGGCATTCGTGGCTGGTGTCATCCG" /Tm="72.3" /%GC="58.6" /Hairpin_Tm="52.6" /Self_Dimer_Tm="13.0" /created_by="chenp" /label="PC1508" CDS 1325..3595 /created_by="chenp" /modified_by="chenp" /label="mMLH1 1-757 genscript codon" misc_feature 1330..3594 /Original_Bases="GAGTAGCGAAGACGTTATCAAAGAGTTCATGCGTTTCAAAG TTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATCGAAGGTGAAGGTGAAGG TCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACCAAAGGTGGTCCGCTG CCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCCAAAGCTTACGTTA AACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAAGGTTTCAAATG GGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAGGACTCCTCC CTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTCCCGTCCG ACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGTATGTA CCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGACGGT GGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTGC CGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACAC CATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACAGGTGC" /label="GAGTAGCGAAGACGTTATCAAAGAGTTCATGCGTTTCAAAGTTCGTATGG AAGGTTCCGTTAACGGTCACGAGTTCGAAATCGAAGGTGAAGGTGAAGGTCGTCCGTA CGAAGGTACCCAGACCGCTAAACTGAAAGTTACCAAAGGTGGTCCGCTGCCGTTCGCT TGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCCAAAGCTTACGTTAAACACCCGG CTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAAGGTTTCAAATGGGAACGTGT TATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAGGACTCCTCCCTGCAAGAC GGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTCCCGTCCGACGGTCCGG TTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGTATGTACCCGGAAGA CGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGACGGTGGTCACTAC GACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTGCCGGGTGCTT ACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACCATCGTTGA ACAGTACGAACGTGCTGAAGGTCGTCACTCCACAGGTGC" /note="Geneious type: Editing History Replacement" primer_bind 1852..1873 /Sequence="CCTTGAAGTGGTCGGACGATAC" /Tm="60.7" /%GC="54.5" /Hairpin_Tm="None" /Self_Dimer_Tm="1.4" /created_by="chenp" /label="PC1512" primer_bind 2599..2618 /Sequence="GGCCACGAGAGAGGATGAAG" /Tm="59.9" /%GC="60.0" /Hairpin_Tm="45.5" /Self_Dimer_Tm="None" /created_by="chenp" /label="PC1513" primer_bind 3292..3311 /Sequence="GCTGCCCATTTTCATCCTCC" /Tm="59.3" /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /created_by="chenp" /label="PC1514" Junction join(3532,3553) /Reads_using=3 /Reads_supporting_discovery=2 /Junction_Type="Deletion" /Deletion_Size=20 /Junction_Source="3532+" /Junction_Destination="3553+" /ID="junction 1" /label="junction 1" primer_bind complement(3566..3603) /Sequence="ACCGGTTAAAAAACTTTGTACAGATCAGGGAGATTGGC" /Tm="63.2" /%GC="42.1" /Hairpin_Tm="32.5" /Self_Dimer_Tm="None" /created_by="chenp" /label="PC1511" USER 3589..3603 /created_by="chenp" primer_bind 3589..3625 /Sequence="AGTTTTTTAACCGGTCATCATCACCATCACCATTGAG" /Tm="62.2" /%GC="40.5" /Hairpin_Tm="29.8" /Self_Dimer_Tm="4.2" /created_by="chenp" /label="PC1510" Terminator 3647..3874 /ApEinfo_revcolor="#ffef86" /ApEinfo_fwdcolor="#ffef86" /label="bGH_PA term" misc_feature complement(3796..3820) /ApEinfo_revcolor="#c7b0e3" /ApEinfo_fwdcolor="#c7b0e3" /label="ACK64" primer_bind complement(3796..3820) /ApEinfo_revcolor="#f58a5e" /ApEinfo_fwdcolor="#f58a5e" /label="ACK64 (end)" primer_bind complement(3810..3829) /ApEinfo_revcolor="#9eafd2" /ApEinfo_fwdcolor="#9eafd2" /label="438_bGH_QPCRR2" primer_bind complement(3815..3833) /ApEinfo_revcolor="#d6b295" /ApEinfo_fwdcolor="#d6b295" /label="439_bGH_QPCRR3" primer_bind complement(3835..3854) /ApEinfo_revcolor="#d59687" /ApEinfo_fwdcolor="#d59687" /label="437_bGH_QPCRR1" primer_bind complement(3855..3874) /ApEinfo_revcolor="#ffef86" /ApEinfo_fwdcolor="#ffef86" /label="920_bGH-BamHI-rev" primer_bind 3933..3959 /ApEinfo_revcolor="#84b0dc" /ApEinfo_fwdcolor="#84b0dc" /label="654_CAG_fwd" primer_bind 3941..3959 /ApEinfo_revcolor="#d59687" /ApEinfo_fwdcolor="#d59687" /label="NMG-1452" primer_bind complement(3945..3961) /ApEinfo_revcolor="#85dae9" /ApEinfo_fwdcolor="#85dae9" /label="M13-R (-26)" primer_bind complement(3945..3961) /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /label="QB0048 M13 Reverse" primer_bind complement(3945..3961) /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" /label="Genewiz M13R" primer_bind complement(3945..3961) /ApEinfo_revcolor="#9eafd2" /ApEinfo_fwdcolor="#9eafd2" /label="Eton M13R Reverse" primer_bind complement(3958..3981) /ApEinfo_revcolor="#c6c9d1" /ApEinfo_fwdcolor="#c6c9d1" /label="M13-R (-46)" primer_bind complement(3958..3981) /ApEinfo_revcolor="#84b0dc" /ApEinfo_fwdcolor="#84b0dc" /label="QB0108 Tac promoter" primer_bind complement(3958..3980) /ApEinfo_revcolor="#84b0dc" /ApEinfo_fwdcolor="#84b0dc" /label="QB0050 M13/pUC Reverse" Promoter complement(3994..4023) /ApEinfo_revcolor="#c6c9d1" /ApEinfo_fwdcolor="#c6c9d1" /label="lac prom" primer_bind 4020..4050 /ApEinfo_revcolor="#b1ff67" /ApEinfo_fwdcolor="#b1ff67" /label="NMG-850" primer_bind complement(4030..4049) /ApEinfo_revcolor="#b1ff67" /ApEinfo_fwdcolor="#b1ff67" /label="Lac-F (EtonSeq)" primer_bind complement(4030..4049) /ApEinfo_revcolor="#85dae9" /ApEinfo_fwdcolor="#85dae9" /label="836_Lac-F" misc_feature complement(4038..4062) /ApEinfo_revcolor="#f58a5e" /ApEinfo_fwdcolor="#f58a5e" /label="ACK32" primer_bind 4038..4062 /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /label="ACK21" primer_bind complement(4038..4062) /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /label="ACK-32" primer_bind 4096..4122 /ApEinfo_revcolor="#c7b0e3" /ApEinfo_fwdcolor="#c7b0e3" /label="NMG-1101" primer_bind complement(4176..4193) /ApEinfo_revcolor="#84b0dc" /ApEinfo_fwdcolor="#84b0dc" /label="QB0039 L4440" primer_bind complement(4228..4247) /ApEinfo_revcolor="#ff9ccd" /ApEinfo_fwdcolor="#ff9ccd" /label="VR" primer_bind complement(4317..4338) /ApEinfo_revcolor="#c6c9d1" /ApEinfo_fwdcolor="#c6c9d1" /label="11_pFYF_Seq_Rev" misc_feature complement(4332..4951) /ApEinfo_revcolor="#c7b0e3" /ApEinfo_fwdcolor="#c7b0e3" /label="pBR322 origin" primer_bind 4347..4371 /ApEinfo_revcolor="#84b0dc" /ApEinfo_fwdcolor="#84b0dc" /label="ACK10" primer_bind complement(4408..4427) /ApEinfo_revcolor="#b1ff67" /ApEinfo_fwdcolor="#b1ff67" /label="BR322-F" misc_feature 4543 /label="/ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9" primer_bind 4555..4582 /ApEinfo_revcolor="#b7e6d7" /ApEinfo_fwdcolor="#b7e6d7" /label="NMG-851" primer_bind 4570..4588 /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" /label="QB0766 RepO-F" primer_bind complement(4645..4671) /ApEinfo_revcolor="#ffef86" /ApEinfo_fwdcolor="#ffef86" /label="NMG-309" primer_bind 4667..4686 /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /label="833_BR322-rev1" primer_bind complement(4695..4714) /ApEinfo_revcolor="#9eafd2" /ApEinfo_fwdcolor="#9eafd2" /label="QB0767 RepO-R" misc_feature 4788 /ApEinfo_revcolor="#d59687" /ApEinfo_fwdcolor="#d59687" /label="G" primer_bind 4848..4872 /ApEinfo_revcolor="#d6b295" /ApEinfo_fwdcolor="#d6b295" /label="ACK11" primer_bind complement(4848..4867) /ApEinfo_revcolor="#85dae9" /ApEinfo_fwdcolor="#85dae9" /label="832_BR322-fwd1" primer_bind 5098..5126 /ApEinfo_revcolor="#f8d3a9" /ApEinfo_fwdcolor="#f8d3a9" /label="NMG-853" Marker complement(5106..5966) /ApEinfo_revcolor="#d6b295" /ApEinfo_fwdcolor="#d6b295" /label="amp marker" primer_bind 5256..5285 /ApEinfo_revcolor="#c7b0e3" /ApEinfo_fwdcolor="#c7b0e3" /label="NMG-584" primer_bind 5347..5371 /ApEinfo_revcolor="#d59687" /ApEinfo_fwdcolor="#d59687" /label="ACK12" primer_bind 5455..5481 /ApEinfo_revcolor="#f58a5e" /ApEinfo_fwdcolor="#f58a5e" /label="NMG-1102" primer_bind 5512..5531 /ApEinfo_revcolor="#f58a5e" /ApEinfo_fwdcolor="#f58a5e" /label="ACK563" primer_bind 5630..5658 /ApEinfo_revcolor="#b7e6d7" /ApEinfo_fwdcolor="#b7e6d7" /label="NMG-854" primer_bind 5729..5748 /ApEinfo_revcolor="#85dae9" /ApEinfo_fwdcolor="#85dae9" /label="QB0005 Amp-R" primer_bind 5790..5819 /ApEinfo_revcolor="#d59687" /ApEinfo_fwdcolor="#d59687" /label="NMG-585" primer_bind complement(5814..5841) /ApEinfo_revcolor="#d59687" /ApEinfo_fwdcolor="#d59687" /label="562_Amp_Fwd" primer_bind 5814..5841 /ApEinfo_revcolor="#d59687" /ApEinfo_fwdcolor="#d59687" /label="563_Amp_Rev" primer_bind 5847..5871 /ApEinfo_revcolor="#c6c9d1" /ApEinfo_fwdcolor="#c6c9d1" /label="ACK13" primer_bind 5948..5977 /ApEinfo_revcolor="#faac61" /ApEinfo_fwdcolor="#faac61" /label="NMG-396" primer_bind 6005..6027 /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /label="NMG-1469" Promoter complement(6008..6036) /ApEinfo_revcolor="#b7e6d7" /ApEinfo_fwdcolor="#b7e6d7" /label="amp prom" primer_bind 6021..6047 /ApEinfo_revcolor="#ff9ccd" /ApEinfo_fwdcolor="#ff9ccd" /label="NMG-1103" primer_bind 6080..6097 /ApEinfo_revcolor="#d59687" /ApEinfo_fwdcolor="#d59687" /label="QB0090 pQE promoter" primer_bind 6087..6115 /ApEinfo_revcolor="#85dae9" /ApEinfo_fwdcolor="#85dae9" /label="NMG-838" primer_bind complement(6150..6169) /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" /label="QB0092 pRS-marker" ORIGIN 1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actgcaaaga tggataaagt 61 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa 121 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 181 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 241 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 301 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 361 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 421 ttacttccac tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg 481 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg 541 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc 601 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg 661 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc 721 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg 781 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc 841 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc 901 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag 961 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc 1021 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga 1081 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat 1141 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta 1201 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac 1261 agtggttcaa agtttttttc ttccatttca ggtgtcgtga cgtacgggat cctctagagc 1321 caccatggca ttcgtggctg gtgtcatccg ccgtcttgat gagactgtcg tcaacagaat 1381 tgccgcagga gaagtcatcc agcgtccagc caacgccatc aaggaaatga tcgaaaactg 1441 tctggatgcc aagagcacaa acatacaagt tgtggtgaag gagggtggcc tcaaactcat 1501 tcagatccaa gacaacggca cgggaattag aaaagaagac ctggacattg tgtgcgagcg 1561 cttcacaacc tctaaattgc agacatttga ggacctggca agcatcagta cttatggctt 1621 ccgaggggag gctctggcct ccataagcca tgtggctcac gtgaccatca cgacaaaaac 1681 ggctgatgga aagtgcgcat accgtgcatc ctactcggat gggaaactac aggcccctcc 1741 aaaaccttgt gccggcaacc agggcacact catcactgtt gaagatttgt tctacaacat 1801 tatcacacgg cggaaggctt taaagaatcc aagtgaggaa tatgggaaga tccttgaagt 1861 ggtcggacga tactctatcc acaactctgg gatttccttc tctgtgaaga agcaaggtga 1921 aactgtttct gatgtgcgca ccttgcccaa tgctaccact gtggacaata ttcgcagcat 1981 ctttgggaat gcggtctctc gagagctcat tgaggttgga tgtgaggaca agacactggc 2041 tttcaagatg aatggctaca tttccaatgc caactacagc gttaaaaagt gtatcttcct 2101 gctgtttatc aaccataggc tggtggagtc agctgcactc cgaaaagcca tagagacagt 2161 atatgctgca tacttgccca aaaacacgca tccattcctc tacctgtcgc tagaaatctc 2221 ccctcagaac gtcgatgtca atgtccaccc caccaagcat gaagttcact tccttcatga 2281 ggaatcaata ctgcaaagag tgcagcaaca tatagaaagc aaattactgg gttccaacag 2341 ttcaagaatg tatttcaccc agactcttct gcctggtctg gcaggcccta gtggagaagc 2401 agcacggccg accactggag tggcctcaag cagtaccagt ggttctggag acaaagtgta 2461 tgcctaccag atggtcagga cagactctcg ggagcagaag ctcgacgctt ttttacagcc 2521 tgtgtcaagc ctgggaccct cccagccaca agatcctgcc ccggtaaggg gagccaggac 2581 agaaggctcc ccagagcggg ccacgagaga ggatgaagag atgctggcgc tcccagcgcc 2641 agctgaggcc gcagctgaat ctgagaatct ggagagagag tctctgatgg agacctccga 2701 tgcagcccag aaagctgcac ccacatcatc tccaggctct tcaaggaaaa ggcacagaga 2761 agactctgat gtagagatgg tcgaaaatgc atcagggaaa gaaatgacag ctgcctgcta 2821 cccgcgtagg agaattataa acctgacaag tgtgttatcg ttgcaggagg agattagtga 2881 aaggtgccac gaaaccttga gagagatgct gaggaatcat agctttgtgg gctgtgtgaa 2941 cccccaatgg gccctggctc agcaccagac caaactatat ttgcttaata ctaccaagct 3001 gtcagaagaa ctgttctatc agatactcat ttatgacttt gccaactttg gagttttgcg 3061 gctgagcgag cccgcgccac tctttgacct agccatgctt gctctggaca gccctgaaag 3121 tggctggact gaagatgacg gcccgaagga agggttggct gagtatattg tagaatttct 3181 gaagaagaaa gcagagatgc tcgccgacta cttctccgta gagatcgacg aggagggcaa 3241 tcttatcgga ttacctctcc tcatagactc ttatgtgccc cctttggagg ggctgcccat 3301 tttcatcctc cgcctcgcta ctgaagtaaa ctgggatgag gagaaggagt gcttcgagag 3361 tcttagtaaa gagtgtgcaa tgttttatag cattaggaag cagtacatcc tggaagagtc 3421 cacactgtct gggcagcagt cagacatgcc tggtagcacc agcaagccat ggaagtggac 3481 tgtggagcac atcatctaca aggccttccg gtcccacctg cttcctccca agcactttac 3541 cgaggatggg aatgtgctcc agctggccaa tctccctgat ctgtacaaag ttttttaacc 3601 ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct 3661 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc 3721 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt 3781 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat 3841 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg 3901 ggctcgatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct 3961 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 4021 aaagcctagg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 4081 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 4141 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 4201 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 4261 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 4321 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 4381 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 4441 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 4501 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 4561 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 4621 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 4681 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 4741 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 4801 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 4861 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 4921 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 4981 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 5041 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 5101 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 5161 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 5221 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 5281 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 5341 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 5401 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 5461 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 5521 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 5581 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 5641 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 5701 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 5761 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 5821 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 5881 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 5941 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 6001 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 6061 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga 6121 tctcccgatc ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc 6181 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa 6241 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt 6301 tttgcgctgc ttcg //