LOCUS       pPC1330                 5108 bp ds-DNA     circular     13-AUG-2020
DEFINITION  [pBR322, Cb] pEF1a-hMLH1 1-335-bpNLS genscript codon.
ACCESSION   urn.local...1v-c94n8qq
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     primer_bind     1..21
                     /Sequence="CGATGTACGGGCCAGATATAC"
                     /%GC="52.4"
                     /Transferred_From="PC0037"
                     /Transferred_Similarity="100.00%"
                     /label="PC0037"
     misc_feature    complement(21..60)
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="EMJ_P0002"
     promoter        43..1300
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="EF-1a"
     misc_feature    43..72
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /label="GH"
     misc_feature    46..73
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="EMJ_P0003"
     misc_feature    46..60
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /label="USER"
     mutation        complement(429..430)
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="G insertion"
     primer_bind     complement(610..638)
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /label="PC0043"
     primer_bind     684..703
                     /Sequence="GCCAAGATCTGCACACTGGT"
                     /Tm="60.6"
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="10.0"
                     /created_by="chenp"
                     /label="BP0033"
     primer_bind     1213..1234
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="BP0132 (EF1a-For)"
     misc_feature    1304..1315
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /label="USER"
     primer_bind     complement(1305..1332)
                     /Sequence="AAGCTCATGGTGGCTCTAGAGGATCCCG"
                     /Tm="69.0"
                     /%GC="57.1"
                     /Hairpin_Tm="37.9"
                     /Self_Dimer_Tm="21.2"
                     /created_by="chenp"
                     /label="PC1367"
     regulatory      1319..1324
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /modified_by="chenp"
                     /label="Kozak"
     primer_bind     1322..1350
                     /Sequence="ACCATGAGCTTCGTTGCTGGAGTCATCCG"
                     /Tm="70.1"
                     /%GC="55.2"
                     /Hairpin_Tm="50.1"
                     /Self_Dimer_Tm="9.4"
                     /created_by="chenp"
                     /label="PC1366"
     CDS             1325..>2335
                     /created_by="chenp"
                     /modified_by="chenp"
                     /label="MLH1 1-337 genscript codon optimized"
     misc_feature    1328..2389
                     /Original_Bases="GCGAGTAGCGAAGACGTTATCAAAGAGTTCATGCGTTTCAA
                     AGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATCGAAGGTGAAGGTGAA
                     GGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACCAAAGGTGGTCCGC
                     TGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCCAAAGCTTACGT
                     TAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAAGGTTTCAAA
                     TGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAGGACTCCT
                     CCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTCCCGTC
                     CGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGTATG
                     TACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGACG
                     GTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCT
                     GCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTAC
                     ACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACAGGTGCTTAA"
                     /label="GCGAGTAGCGAAGACGTTATCAAAGAGTTCATGCGTTTCAAAGTTCGTAT
                     GGAAGGTTCCGTTAACGGTCACGAGTTCGAAATCGAAGGTGAAGGTGAAGGTCGTCCG
                     TACGAAGGTACCCAGACCGCTAAACTGAAAGTTACCAAAGGTGGTCCGCTGCCGTTCG
                     CTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCCAAAGCTTACGTTAAACACCC
                     GGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAAGGTTTCAAATGGGAACGT
                     GTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAGGACTCCTCCCTGCAAG
                     ACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTCCCGTCCGACGGTCC
                     GGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGTATGTACCCGGAA
                     GACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGACGGTGGTCACT
                     ACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTGCCGGGTGC
                     TTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACCATCGTT
                     GAACAGTACGAACGTGCTGAAGGTCGTCACTCCACAGGTGCTTAA"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     complement(1396..1431)
                     /Sequence="ATCATCTCCTTTATTGCATTGGCAGGTCTCTGGATC"
                     /Tm="69.0"
                     /%GC="44.4"
                     /Hairpin_Tm="53.1"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1365"
     USER            1418..1431
                     /created_by="chenp"
     primer_bind     1418..1450
                     /Sequence="ATAAAGGAGATGATCGAGAACTGTCTGGACGCC"
                     /Tm="68.7"
                     /%GC="48.5"
                     /Hairpin_Tm="40.9"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1364"
     primer_bind     complement(2308..2333)
                     /Sequence="AGCCCAGCAGCTTGCTCTCAATGTGC"
                     /Tm="69.8"
                     /%GC="57.7"
                     /Hairpin_Tm="55.3"
                     /Self_Dimer_Tm="10.0"
                     /created_by="chenp"
                     /label="PC1385"
     USER            2322..2333
                     /created_by="chenp"
     primer_bind     2322..2347
                     /Sequence="AGCTGCTGGGCTCTAAACGGACAGCC"
                     /Tm="70.5"
                     /%GC="61.5"
                     /Hairpin_Tm="49.3"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1384"
     CDS             2336..2389
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="MKRTADGSEFESPKKKRKV"
                     /label="SV40 NLS"
     primer_bind     complement(2367..2403)
                     /Sequence="ATGATGACCGGTTAGACTTTCCGCTTCTTCTTTGGTG"
                     /Tm="64.3"
                     /%GC="45.9"
                     /Hairpin_Tm="39.6"
                     /Self_Dimer_Tm="5.7"
                     /created_by="chenp"
                     /label="PC1379"
     USER            2392..2403
                     /created_by="chenp"
     primer_bind     2392..2419
                     /Sequence="ACCGGTCATCATCACCATCACCATTGAG"
                     /Tm="66.6"
                     /%GC="50.0"
                     /Hairpin_Tm="34.4"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1378"
     Terminator      2441..2668
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="bGH_PA term"
     misc_feature    complement(2590..2614)
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /label="ACK64"
     primer_bind     complement(2590..2614)
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="ACK64 (end)"
     primer_bind     complement(2604..2623)
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /label="438_bGH_QPCRR2"
     primer_bind     complement(2609..2627)
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="439_bGH_QPCRR3"
     primer_bind     complement(2629..2648)
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="437_bGH_QPCRR1"
     primer_bind     complement(2649..2668)
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="920_bGH-BamHI-rev"
     primer_bind     2727..2753
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /label="654_CAG_fwd"
     primer_bind     2735..2753
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="NMG-1452"
     primer_bind     complement(2739..2755)
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="M13-R (-26)"
     primer_bind     complement(2739..2755)
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="QB0048 M13 Reverse"
     primer_bind     complement(2739..2755)
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /label="Genewiz M13R"
     primer_bind     complement(2739..2755)
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /label="Eton M13R Reverse"
     primer_bind     complement(2752..2775)
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /label="M13-R (-46)"
     primer_bind     complement(2752..2775)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /label="QB0108 Tac promoter"
     primer_bind     complement(2752..2774)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /label="QB0050 M13/pUC Reverse"
     Promoter        complement(2788..2817)
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /label="lac prom"
     primer_bind     2814..2844
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /label="NMG-850"
     primer_bind     complement(2824..2843)
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /label="Lac-F (EtonSeq)"
     primer_bind     complement(2824..2843)
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="836_Lac-F"
     misc_feature    complement(2832..2856)
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="ACK32"
     primer_bind     2832..2856
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="ACK21"
     primer_bind     complement(2832..2856)
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="ACK-32"
     primer_bind     2890..2916
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /label="NMG-1101"
     primer_bind     complement(2970..2987)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /label="QB0039 L4440"
     primer_bind     complement(3022..3041)
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="VR"
     primer_bind     complement(3111..3132)
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /label="11_pFYF_Seq_Rev"
     misc_feature    complement(3126..3745)
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /label="pBR322 origin"
     primer_bind     3141..3165
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /label="ACK10"
     primer_bind     complement(3202..3221)
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /label="BR322-F"
     misc_feature    3337
                     /label="/ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9"
     primer_bind     3349..3376
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /label="NMG-851"
     primer_bind     3364..3382
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /label="QB0766 RepO-F"
     primer_bind     complement(3426..3454)
                     /Sequence="ACTCAAGACGAUAGTTACCGGATAAGGCG"
                     /%GC="48.3"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0970"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:2e-b64q18g"">PC0970</a>"
                     /label="PC0970"
     primer_bind     complement(3439..3465)
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="NMG-309"
     primer_bind     3443..3469
                     /Sequence="ATCGTCTTGAGUCCAACCCGGTAAGAC"
                     /%GC="51.9"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0969"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-b64q18e"">PC0969</a>"
                     /label="PC0969"
     primer_bind     3461..3480
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="833_BR322-rev1"
     primer_bind     complement(3489..3508)
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /label="QB0767 RepO-R"
     misc_feature    3582
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="G"
     primer_bind     3642..3666
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="ACK11"
     primer_bind     complement(3642..3661)
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="832_BR322-fwd1"
     primer_bind     3892..3920
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /label="NMG-853"
     Marker          complement(3900..4760)
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="amp marker"
     primer_bind     4050..4079
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /label="NMG-584"
     primer_bind     4141..4165
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="ACK12"
     primer_bind     4249..4275
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="NMG-1102"
     primer_bind     4306..4325
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="ACK563"
     primer_bind     4424..4452
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /label="NMG-854"
     primer_bind     4523..4542
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="QB0005 Amp-R"
     primer_bind     4584..4613
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="NMG-585"
     primer_bind     complement(4608..4635)
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="562_Amp_Fwd"
     primer_bind     4608..4635
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="563_Amp_Rev"
     primer_bind     4641..4665
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /label="ACK13"
     primer_bind     4742..4771
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /label="NMG-396"
     primer_bind     4799..4821
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="NMG-1469"
     Promoter        complement(4802..4830)
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /label="amp prom"
     primer_bind     4815..4841
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="NMG-1103"
     primer_bind     4874..4891
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="QB0090 pQE promoter"
     primer_bind     4881..4909
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="NMG-838"
     primer_bind     complement(4944..4963)
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /label="QB0092 pRS-marker"
ORIGIN      
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actgcaaaga tggataaagt
       61 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa
      121 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
      181 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
      241 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
      301 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
      361 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
      421 ttacttccac tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
      481 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
      541 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
      601 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
      661 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
      721 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
      781 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
      841 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
      901 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
      961 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     1021 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     1081 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     1141 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     1201 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     1261 agtggttcaa agtttttttc ttccatttca ggtgtcgtga cgtacgggat cctctagagc
     1321 caccatgagc ttcgttgctg gagtcatccg gagactggac gagacagtgg tgaacagaat
     1381 tgccgccggc gaggtgatcc agagacctgc caatgcaata aaggagatga tcgagaactg
     1441 tctggacgcc aagtccacaa gcattcaggt gatcgtgaag gagggcggac tgaagctgat
     1501 ccagatccaa gacaacggca caggcatcag aaaggaagat ctggacatcg tgtgtgaacg
     1561 gttcaccaca tctaagctgc agtcttttga ggatctggcc tctatcagta cctacggctt
     1621 cagaggcgag gccctggcca gcatcagcca cgtggcccat gtgaccatca ccaccaaaac
     1681 cgccgacggc aaatgcgctt atcgcgctag ctacagcgac ggcaagctga aagccccgcc
     1741 aaagccttgc gccggcaacc agggtacaca gataacagtg gaggatctgt tctacaacat
     1801 cgccacccgg agaaaggccc tgaaaaatcc cagcgaggag tacggcaaga tcctggaagt
     1861 cgtcggcaga tactccgtgc acaacgccgg aatcagcttt agcgtaaaga agcagggaga
     1921 aaccgtggcc gatgtgcgca ccctgccaaa tgccagcacc gtggataaca tcagaagcat
     1981 tttcggaaat gccgtgtcca gagaactgat cgagatcggc tgcgaagata agaccctggc
     2041 ttttaagatg aacggctaca tcagcaacgc caattactct gtgaagaagt gcatctttct
     2101 tctgttcatc aaccacagac tggtggaaag caccagcctg cggaaagcca tcgagacagt
     2161 gtacgccgcc tacctgccta agaacaccca ccccttcctg tacctgagcc tcgagatcag
     2221 ccctcagaac gtggacgtca atgtgcatcc tacaaagcac gaggtgcact tcctgcacga
     2281 ggaaagcatc ctggaaagag tgcagcagca cattgagagc aagctgctgg gctctaaacg
     2341 gacagccgac ggaagcgagt tcgagtcacc aaagaagaag cggaaagtct aaccggtcat
     2401 catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     2461 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     2521 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     2581 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     2641 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg
     2701 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     2761 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     2821 tagggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     2881 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     2941 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3001 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3061 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3121 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3181 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3241 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3301 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3361 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3421 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3481 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3541 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3601 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3661 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3721 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3781 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3841 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3901 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3961 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     4021 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4081 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     4141 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4201 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4261 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4321 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4381 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4441 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4501 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4561 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4621 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4681 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4741 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4801 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4861 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc
     4921 gatcccctag ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     4981 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     5041 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
     5101 ctgcttcg
//
