LOCUS       pPC1000                 9181 bp    DNA     circular UNA 29-JUL-2021
DEFINITION  [ColE1, Cb] LentiTransfer EF1a-PURO-T2A-BFP
            mU6-EGFP_sgRNA-PEtarget_HBB_site.
ACCESSION   urn.local...8z-b611tk4
VERSION     urn.local...8z-b611tk4
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     primer_bind     221..241
                     /Sequence="CGATGTACGGGCCAGATATAC"
                     /%GC="52.4"
                     /Transferred_From="PC0037"
                     /Transferred_Similarity="100.00%"
                     /label="PC0037"
     promoter        263..826
                     /label="5' CMV promoter"
     primer_bind     781..801
                     /%GC="66.7"
                     /Hairpin_Tm="52.4"
                     /Self_Dimer_Tm="3.5"
                     /Tm="67.0"
                     /Sequence="CGCAAATGGGCGGTAGGCGTG"
                     /created_by="primer3"
                     /Transferred_From="BP0002 (CMV-For)"
                     /Transferred_Similarity="100.00%"
                     /label="BP0002 (CMV-For)"
     LTR             844..1024
                     /label="HIV-1 LTR 5'"
     misc_feature    1126..1170
                     /label="HIV-1_psi_pack"
     primer_bind     1423..1445
                     /Sequence="CAGTAGCAACCCTCTATTGTGTG"
                     /Tm="59.1"
                     /%GC="47.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1003"
     misc_feature    1695..1928
                     /label="HIV-1 RRE"
     primer_bind     2069..2098
                     /Sequence="GCTTAATACACTCCTTAATTGAAGAATCGC"
                     /Tm="61.3"
                     /%GC="36.7"
                     /Hairpin_Tm="37.3"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1004"
     misc_feature    2450..2473
                     /label="HIV-1 cPPT"
     promoter        2631..2944
                     /modified_by="chenp"
                     /label="mouse U6 promoter - modified"
     primer_bind     2733..2759
                     /Sequence="CTAGTAACTATAGAGGCTTAATGTGCG"
                     /Tm="59.2"
                     /%GC="40.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="6.8"
                     /created_by="chenp"
                     /label="PC1005"
     misc_feature    2856..2861
                     /label="mU6 TATA box"
     primer_bind     2921..2944
                     /Sequence="ATCCCTTGGAGAACCACCTTGTTG"
                     /Tm="62.8"
                     /%GC="50.0"
                     /Hairpin_Tm="46.8"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="Britt's F HTS primer"
     misc_feature    2945..2964
                     /label="EGFP_NT2"
     misc_feature    2965..3050
                     /modified_by="chenp"
                     /label="modified F+E sgRNA scaffold"
     misc_feature    3051..3056
                     /label="polyT-track"
     misc_feature    3063..3089
                     /label="Cas9 cutting target sequence (HBB seq)"
     misc_feature    3063..3089
                     /label="custom R1 primer"
     primer_bind     3063..3089
                     /created_by="chenp"
                     /Sequence="GCCTTTAGTGATGGCCTGGCTCACCTG"
                     /Tm="69.1"
                     /%GC="59.3"
                     /Hairpin_Tm="41.5"
                     /Self_Dimer_Tm="27.7"
                     /Color="#00ff00"
                     /modified_by="chenp"
                     /label="PC1011"
     misc_feature    3090..3323
                     /modified_by="chenp"
                     /label="pSL34 target"
     misc            complement(3146..3151)
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="NNGRRT"
                     /annotation_group="NNGRRT: 3,146 <- 3,151"
                     /label="SaPAM"
     misc            complement(3152..3173)
                     /created_by="chenp"
                     /modified_by="chenp"
                     /label="pSL34 guide 1"
     primer_bind     complement(3160..3191)
                     /created_by="chenp"
                     /Sequence="ATCAGATGTAGTTCTTTGGAGAACGTCCTAAC"
                     /Tm="64.4"
                     /%GC="40.6"
                     /Hairpin_Tm="56.7"
                     /Self_Dimer_Tm="12.2"
                     /modified_by="chenp"
                     /label="PC1017"
     USER            3180..3191
                     /created_by="chenp"
                     /label="USER"
     primer_bind     3180..3207
                     /Sequence="ACTACATCTGATAGGCAGCCTGCACTGG"
                     /Tm="67.8"
                     /%GC="53.6"
                     /Hairpin_Tm="47.3"
                     /Self_Dimer_Tm="25.6"
                     /created_by="chenp"
                     /label="PC1016"
     misc_feature    3186..3243
                     /Original_Bases="GTTTCAAAGTGTTTGCCCACGTCAGGGCCAAGGATGAAGAA
                     GCTGCATTTGCAAGATT"
                     /label="GTTTCAAAGTGTTTGCCCACGTCAGGGCCAAGGATGAAGAAGCTGCATTT
                     GCAAGATT"
                     /note="Geneious type: Editing History Replacement"
     misc            3186..3245
                     /created_by="chenp"
                     /modified_by="chenp"
                     /label="HBB 3'UTR"
     misc            3186..3207
                     /created_by="chenp"
                     /modified_by="chenp"
                     /label="pAX198 guide 1"
     misc            3208..3213
                     /created_by="chenp"
                     /label="Sa PAM"
     primer_bind     complement(3225..3275)
                     /Sequence="ACGGTTCAACAAAATATTGTCGAGAGGGGTGTGTGCTGGCCTATCAC
                     TTTG"
                     /Tm="69.8"
                     /%GC="47.1"
                     /Hairpin_Tm="43.7"
                     /Self_Dimer_Tm="15.2"
                     /created_by="chenp"
                     /label="PC01018"
     misc            complement(3246..3251)
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="NNGRRT"
                     /annotation_group="NNGRRT: 3,234 <- 3,239"
                     /label="SaPAM"
     misc_feature    3247..3273
                     /Original_Bases="AAAGGCAGGTATCACCTATGGTACTTG"
                     /label="AAAGGCAGGTATCACCTATGGTACTTG"
                     /note="Geneious type: Editing History Replacement"
     misc            complement(3252..3273)
                     /created_by="chenp"
                     /modified_by="chenp"
                     /label="pSL32 guide 2"
     USER            3261..3275
                     /created_by="chenp"
                     /label="USER"
     primer_bind     3261..3302
                     /Sequence="ATTTTGTTGAACCGTACGAAAAATTTGCCTCAAATATTAAAG"
                     /Hairpin_Tm="30.1"
                     /Tm="59.9"
                     /Self_Dimer_Tm="15.6"
                     /%GC="28.6"
                     /created_by="primer3"
                     /label="PC0968"
     misc_feature    3275
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     complement(3329..3348)
                     /Sequence="TAGGCGGCCGCTGCACGTAG"
                     /Tm="67.4"
                     /%GC="70.0"
                     /Hairpin_Tm="41.4"
                     /Self_Dimer_Tm="45.2"
                     /Color="#00ff00"
                     /created_by="chenp"
                     /label="PC1015"
     primer_bind     complement(3353..3373)
                     /created_by="chenp"
                     /Sequence="CAACCAACGCTATCGGCATGG"
                     /Tm="62.3"
                     /%GC="57.1"
                     /Hairpin_Tm="46.6"
                     /Self_Dimer_Tm="6.3"
                     /modified_by="chenp"
                     /label="Britt's R HTS primer"
     promoter        3408..4579
                     /label="EF1a promoter"
     primer_bind     complement(3889..3917)
                     /Sequence="GTCATCAAAAATTTTAAATGGCTAGAGAC"
                     /%GC="31.0"
                     /Transferred_From="PC0043"
                     /Transferred_Similarity="100.00%"
                     /label="PC0043"
     primer_bind     4492..4513
                     /%GC="36.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="57.2"
                     /Sequence="ATTTGCCCTTTTTGAGTTTGGA"
                     /created_by="primer3"
                     /Transferred_From="BP0132 (EF1a-For)"
                     /Transferred_Similarity="100.00%"
                     /label="BP0132 (EF1a-For)"
     primer_bind     complement(4559..4580)
                     /Sequence="CTCACGACACCTGAAATGGAAG"
                     /Tm="59.3"
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1006"
     CDS             4591..5187
                     /modified_by="chenp"
                     /label="PURO resistance gene"
     CDS             5188..5250
                     /modified_by="chenp"
                     /label="T2A"
     primer_bind     5202..5223
                     /Sequence="CAGAGGAAGTCTGCTAACATGC"
                     /Tm="59.1"
                     /%GC="50.0"
                     /Hairpin_Tm="46.4"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1007"
     CDS             5263..5958
                     /modified_by="chenp"
                     /label="BFP"
     primer_bind     5874..5895
                     /Sequence="CAACAACGAGACCTACGTCGAG"
                     /Tm="61.0"
                     /%GC="54.5"
                     /Hairpin_Tm="44.3"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1008"
     misc_feature    6063..6650
                     /label="wPRE"
     primer_bind     6547..6566
                     /created_by="chenp"
                     /Sequence="CTCAATCCAGCGGACCTTCC"
                     /Tm="60.5"
                     /%GC="60.0"
                     /Hairpin_Tm="33.0"
                     /Self_Dimer_Tm="None"
                     /modified_by="chenp"
                     /label="PC1009"
     misc_feature    6841..6864
                     /label="HIV-1 cPPT"
     LTR             7187..7367
                     /label="HIV-1 3' LTR"
     misc_feature    7260..7265
                     /label="poly A signal"
     rep_origin      complement(7411..8093)
                     /direction="LEFT"
                     /label="ColE1 origin"
     primer_bind     complement(7416..7437)
                     /Sequence="CTATGGAAAAACGCCAGCAACG"
                     /Tm="61.0"
                     /%GC="50.0"
                     /Hairpin_Tm="39.1"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /label="PC1010"
     primer_bind     complement(7714..7742)
                     /Sequence="ACTCAAGACGATAGTTACCGGATAAGGCG"
                     /Hairpin_Tm="58.7"
                     /Tm="65.8"
                     /Self_Dimer_Tm="9.5"
                     /%GC="48.3"
                     /created_by="primer3"
                     /label="PC0970"
     USER            7731..7742
                     /created_by="chenp"
                     /label="USER"
     primer_bind     7731..7757
                     /Sequence="ATCGTCTTGAGTCCAACCCGGTAAGAC"
                     /Hairpin_Tm="38.2"
                     /Tm="66.1"
                     /Self_Dimer_Tm="13.7"
                     /%GC="51.9"
                     /created_by="primer3"
                     /label="PC0969"
     CDS             complement(8191..8850)
                     /label="AmpR"
     promoter        complement(9090..9118)
                     /label="AmpR promoter"
ORIGIN      
        1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct
     1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc ggccgcttgg
     1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
     2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc
     2641 gccatctcta ggcccgcgcc ggccccctcg cacggacttg tgggagaagc tcggctactc
     2701 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga
     2761 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat
     2821 ttctataact tcgtatagca tacattatac gaagttataa acagcacaaa aggaaactca
     2881 ccctaactgt aaagtaattg tgtgttttga gactataagt atcccttgga gaaccacctt
     2941 gttggaccag gatgggcacc acccgtttaa gagctaagct ggaaacagca tagcaagttt
     3001 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttctcg
     3061 aggcctttag tgatggcctg gctcacctgg aaaaacaaca attaatgtgt ttgttaccgg
     3121 tgtcacagga tttctgggct cctacatcct tgcagatttg ttaggacgtt ctccaaagaa
     3181 ctacatctga taggcagcct gcactggtgg ggtgaattct ttgccaaagt gataggccag
     3241 cacacacccc tctcgacaat attttgttga accgtacgaa aaatttgcct caaatattaa
     3301 agttgtatta ggcgatttat ctagcatgct acgtgcagcg gccgcctagg atccatgccg
     3361 atagcgttgg ttgagtggat aaccgtatta ccgccaagct tatgcatgtg cccgtcagtg
     3421 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     3481 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     3541 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     3601 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     3661 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     3721 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     3781 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     3841 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     3901 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     3961 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     4021 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     4081 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     4141 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     4201 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     4261 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     4321 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcgc gcttttggag
     4381 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     4441 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     4501 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     4561 tccatttcag gtgtcgtgag ctagcccacc atgaccgagt acaagcccac ggtgcgcctc
     4621 gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac
     4681 cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa
     4741 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     4801 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     4861 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     4921 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     4981 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     5041 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     5101 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     5161 tggtgcatga cccgcaagcc cggtgccgga tcgggagagg gcagaggaag tctgctaaca
     5221 tgcggtgacg tcgaggagaa tcctggccca ccggtcgcca ccagcgagct gattaaggag
     5281 aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt caagtgcaca
     5341 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag
     5401 ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta cggcagcaag
     5461 accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt ccctgagggc
     5521 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac
     5581 accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttcaca
     5641 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac cgagacgctg
     5701 taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg
     5761 agccatctga tcgcaaacat caagaccaca tatagatcca agaaacccgc taagaacctc
     5821 aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga ggccaacaac
     5881 gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct ccctagcaaa
     5941 ctggggcaca agcttaatta agaattcgtc gagggaccta ataacttcgt atagcataca
     6001 ttatacgaag ttatacatgt ttaagggttc cggttccact aggtacaatt cgatatcaag
     6061 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6121 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6181 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6241 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6301 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6361 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6421 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     6481 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6541 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6601 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     6661 cgataccgtc gacctcgatc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     6721 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     6781 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     6841 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     6901 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     6961 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     7021 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
     7081 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
     7141 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
     7201 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7261 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7321 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca tgtgagcaaa
     7381 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7441 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7501 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7561 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7621 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7681 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7741 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7801 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7861 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7921 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7981 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8041 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8101 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8161 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8221 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8281 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8341 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8401 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8461 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8521 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8581 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8641 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8701 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8761 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8821 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8881 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8941 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9001 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9061 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9121 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9181 c
//
