LOCUS       Exported                8923 bp ds-DNA     circular SYN 23-JUN-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Yan Cui
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 2, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8923
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2229..2319
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     enhancer        2418..2721
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2722..2925
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    2974..2991
                     /label=DPE
     CDS             3061..3759
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     misc_feature    3786..4358
                     /label=IRES
     CDS             4361..4963
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    5193..5781
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             5988..6221
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6328..6439
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6650..7238)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7409..8269)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8270..8374)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8446..8581
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8622..8756
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 tactagtaat atttgcatgt cgctatgtgt tctgggaaat caccataaac gtgaaatgtc
     2281 tttggatttg ggaatcttat aagttctgta tgagaccaca tcgattcttt ttttctagac
     2341 gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc
     2401 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     2461 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2521 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2581 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2641 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2701 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2761 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2821 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2881 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
     2941 cagatcgcct ggagacgcca tccagcgctg ttttgacctc catagaagac accgggaccg
     3001 atccagcctc cgcggccggg aacggtgcat tggaacgcgg attccccgtg tacagccacc
     3061 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc
     3121 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccga gcagggccgc
     3181 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc
     3241 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc
     3301 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac
     3361 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac
     3421 ttcaaggtga tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc
     3481 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgatct ggatggcagc
     3541 ttcacccgca ccttcagcct gcgcgacggc ggctactaca gctccgtggt ggacagccac
     3601 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc
     3661 ttccgccgcg tggaggagga tcacagcaac accgagctgg gcatcgtgga gtaccagcac
     3721 gccttcaaga ccccggatgc agatgccggt gaagaataat gtacaagtag cggccgcaaa
     3781 ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     3841 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     3901 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     3961 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     4021 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     4081 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     4141 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     4201 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     4261 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     4321 cacggggacg tggttttcct ttgaaaaaca cgataatacc atggccaccg agtacaagcc
     4381 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc
     4441 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt
     4501 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     4561 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     4621 ggtgttcgcc gagatcggct cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     4681 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     4741 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     4801 cggagtggag gcggccgagc gcgctggggt gcccgccttc ctggagacct ccgcgccccg
     4861 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     4921 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtttgtt tcgaatgagg
     4981 cttcagtact ttacagaatc gttgcctgca catcttggaa acacttgctg ggattacttc
     5041 ttcaggttaa cccaacagaa ttctcttctg aagaaaatat atttcttttt attcatagct
     5101 cttctcgagg taaccggatc caaccagttg tgttttaatg tatattaatg ttactaatgt
     5161 gttttattta aattgagtca cgcgtctgga acaatcaacc tctggattac aaaatttgtg
     5221 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     5281 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     5341 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     5401 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     5461 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     5521 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     5581 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     5641 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     5701 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     5761 ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg
     5821 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     5881 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     5941 caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat
     6001 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     6061 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6121 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6181 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     6241 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca
     6301 ttgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     6361 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     6421 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     6481 tggggcagga cagcaagggg gaggattggg aagacaatag cagggtatca gctcactcaa
     6541 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6601 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6661 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6721 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6781 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6841 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6901 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6961 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7021 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7081 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7141 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7201 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7261 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7321 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7381 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7441 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7501 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7561 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7621 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7681 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7741 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7801 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7861 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7921 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7981 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8041 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8101 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8161 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8221 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8281 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8341 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8401 acgtcgagtt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     8461 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8521 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8581 ctaggctttt gcaaaaagct tcgtcgacgg atcgggagat caacttgttt attgcagctt
     8641 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8701 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
     8761 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
     8821 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
     8881 aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//
