LOCUS       13_MmPeg10.rc4.4        7075 bp ds-DNA     circular     16-SEP-2021
DEFINITION  .
ACCESSION   urn.local...62g-dp4mq1u
VERSION     urn.local...62g-dp4mq1u
KEYWORDS    "accession:addgene_12259_251049".
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct.
FEATURES             Location/Qualifiers
     primer_bind     complement(40..59)
                     /standard_name="SV40pro-F"
     enhancer        161..540
     promoter        541..744
     primer_bind     695..715
                     /standard_name="CMV-F"
     primer_bind     741..765
                     /standard_name="LNCX"
     intron          878..1353
                     /standard_name="-beta-globin intron"
     misc_feature    1451..4335
                     /standard_name="Peg10.rc4"
     misc_feature    2944..3468
                     /standard_name="tiling_4"
     misc_feature    4306..4332
                     /standard_name="HA-TAG"
     polyA_site      4417..4811
     primer_bind     complement(5150..5167)
                     /standard_name="L4440"
     rep_origin      complement(5321..5909)
     primer_bind     complement(5401..5420)
                     /standard_name="pBR322ori-F"
     exon            complement(6080..6940)
                     /standard_name="Translation 6940-6080"
     CDS             complement(6080..6940)
                     /standard_name="AmpR"
     primer_bind     6703..6722
                     /standard_name="Amp-R"
     promoter        complement(6941..7045)
ORIGIN      
        1 ggatcccctg agggggcccc catgggctag aggatccggc ctcggcctct gcataaataa
       61 aaaaaattag tcagccatga gcttggccca ttgcatacgt tgtatccata tcataatatg
      121 tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt
      181 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      241 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      301 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      361 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      421 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      481 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      541 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      601 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      661 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      721 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      781 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt
      841 acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg
      901 ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag
      961 ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct
     1021 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc
     1081 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat
     1141 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg
     1201 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct
     1261 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta
     1321 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg
     1381 ctggcccatc actttggcaa agcacgtgag atctgaattc cccaagctgg ctagcgttta
     1441 aaaccgccac atggctgctg caggtggttc ctccaactgc ccgccccctc cccctccccc
     1501 tcctcccaac aacaacaaca acaacaacac cccaaagagc ccaggcgtgc ctgacgccga
     1561 agatgatgat gaacgcagac acgatgagct ccctgaagac atcaacaact ttgacgaaga
     1621 catgaacagg cagtttgaga atatgaacct gctggatcag gtggagttgc ttgcacagag
     1681 ctacagtctg ctggatcatt tagatgactt tgatgatgat gatgaagacg atgactttga
     1741 tccagaacct gaccaggatg agctccctga gtacagtgac gatgatgacc tggagcttca
     1801 gggtgctgca gcagccccta tcccaaactt tttctccgat gatgactgcc ttgaagacct
     1861 tcctgagaag ttcgatggca accctgacat gctgggtcct ttcatgtatc agtgccagct
     1921 cttcatggaa aagagcacca gagatttctc agttgaccgc atccgtgtgt gcttcgtgac
     1981 aagcatgctg atcggccgtg ccgcccgctg ggctactgcc aagctgcaaa gatgtactta
     2041 cctgatgcac aactacactg cctttatgat ggagctgaag catgtctttg aagaccctca
     2101 gagacgtgaa gctgccaaac gcaagatcag acgtctgcgc cagggccctg ggcctgttgt
     2161 ggactactcc aatgcattcc agatgattgc ccaggacctg gattggactg agcctgccct
     2221 gatggatcag ttccaggaag gtctcaaccc agacattcgc gcagagctgt ctcgccagga
     2281 ggcccccaag accctggctg ctctgattac tgcctgtatt cacatcgaga gaaggctggc
     2341 tcgtgacgct gctgcaaagc ccgatccttc acccagagcc ttggtgatgc ctccaaacag
     2401 ccagaccgat cccaccgagc ctgtgggagg tgcccgcatg cgcctgtcca aggaagaaaa
     2461 ggagagacgc cgcaaaatga atttgtgtct ctactgtggc aatggaggcc atttcgccga
     2521 cacgtgtcca gcgaaagcct ccaagaattc gccgccggga aactccccgg ccccgctgta
     2581 gggggacctt cagcgacagg gccagaacga ataaggtccc caccctccga ggcttcgact
     2641 cagcacctgc aagtgatgct ccagattcat atgccgggca gacccaccct gtttgtccga
     2701 gctatgattg attctggtgc atctggcaac ttcattgatc aagactttgt catacaaaat
     2761 gcaattcctc tcagaatcaa agactggcca gtgatggtgg aagctattga tgggcatcca
     2821 attgcctcgg gcccaatcat tttggaaacc caccacctga tagttgatct gggagaccac
     2881 cgtgagatac tgtcatttga tgtgactcag tctccattct ttcctattgt cctaggaatt
     2941 cgttggctct ctactcacga cccacacatc acctggagta ctcgctccat agtcttcaac
     3001 tctgattact gtcggttgag atgccgaatg ttcgctcaga tcccaagcaa cctgctcttt
     3061 accgttcccc aaccaaatct ccacccctat ctcctgcatc atgtacaccc ccatgtacac
     3121 cctcacatgc atcaacacct tcatcaacac ctgcatcagt ttcttcatcc tgatccacat
     3181 cagtatccac acccagaccc ccattaccat catcaccagc aggctgatat gcagcatcag
     3241 ctgcaacagt atttgtatca gtacttgtac taccatctgt accctgtcat gcatcatcat
     3301 cttcctcctg accaacatga acatctgcac gaatatcttc accaatacct ccatcagtac
     3361 cttcaccaat tcctccacca ccatcttcac cctgacttgc accaatattt gtaccagtat
     3421 cttcacaacc acatgaatcc agatccacat caccatcccc atccagatcc ccctcaggat
     3481 ccacatcacc ctccacatca ggatccccat caggatcctc cacatcagga tccacatcag
     3541 gatgcacatc aggatcccca tatggatcca cacctgcatc agcaccagca tccgcagccg
     3601 cagccgcatc cacaacagca tcctaaccat cctcagcagc caccattctt ctaccacatg
     3661 gctggattca gaatttacca ccctgtaagg tattactata ttcagaatgt gtatacacct
     3721 gttgatgagc atgtctatcc gggtcaccgg gtggttgacc ctaacattga gatgattcct
     3781 ggagcgcaca gcctgcccag tggacatttg tactcaatgt ctgagtctga aatgaatgct
     3841 ctgcgaaatt tcgtggacag gaatgttaaa gatgggctca tgactcccac tgtggcgccc
     3901 aatggagccc aagtcctgca agtgaaaaga gggtggaaac tccaagtcac ttacaattgc
     3961 cgagctccac agagtggcac catccaaaat cagtacctac gcatgtctct tccaaatatg
     4021 ggagaccctg cacacctggc aagctatggt gaatttgtcc aagttcctgg ctacccatat
     4081 ccagcctatg tttactatac aagcccgcat atgatgactg cgtggtaccc agtaggacga
     4141 gatgtacatg gacgaataat cgttgtgcct gttgtaatca cctggtctca aaatacgaac
     4201 cgccagcctc cggtgcccca gtatcctcct ccgcagccac ctccaccacc accaccacct
     4261 ccaccgccac caccacctcc accagcatca tcctgcagtg ctgcgtatcc gtatgatgtt
     4321 ccggattatg catgagaatt caccccacca gtgcaggctg cctatcagaa agtggtggct
     4381 ggtgtggcta atgccctggc ccacaagtat cactaagctc gctttcttgc tgtccaattt
     4441 ctattaaagg ttcctttgtt ccctaagtcc aactactaaa ctgggggata ttatgaaggg
     4501 ccttgagcat ctggattctg cctaataaaa aacatttatt ttcattgcaa tgatgtattt
     4561 aaattatttc tgaatatttt actaaaaagg gaatgtggga ggtcagtgca tttaaaacat
     4621 aaagaaatga agagctagtt caaaccttgg gaaaatacac tatatcttaa actccatgaa
     4681 agaaggtgag gctgcaaaca gctaatgcac attggcaaca gcccctgatg cctatgcctt
     4741 attcatccct cagaaaagga ttcaagtaga ggcttgattt ggaggttaaa gttttgctat
     4801 gctgtatttt acattactta ttgttttagc tgtcctcatg aatgtctttt cactacccat
     4861 ttgcttatcc tgcatctctc agccttgact ccactcagtt ctcttgctta gagataccac
     4921 ctttcccctg aagtgttcct tccatgtttt acggcgagat ggtttctcct cgcctggcca
     4981 ctcagcctta gttgtctctg ttgtcttata gaggtctact tgaagaagga aaaacagggg
     5041 gcatggtttg actgtcctgt gagcccttct tccctgcctc ccccactcac agtgacccgg
     5101 aatccctcga catggcagtc tagcactagt gcggccgcag atctgcttcc tcgctcactg
     5161 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5221 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5281 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5341 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5401 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5461 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5521 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5581 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5641 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5701 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5761 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5821 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5881 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5941 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6001 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6061 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6121 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6181 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6241 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6301 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6361 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6421 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6481 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6541 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6601 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6661 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6721 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6781 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6841 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6901 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6961 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7021 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgt
//
