LOCUS       pCMV-HsPeg10.rc3                6424 bp DNA     circular SYN 05-AUG-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Segel, M., Lash, B., Song, J., Ladha, A., Liu, C.C., Jin, X., Mekhedov, S., Macrae, R.K., Koonin, E.V., Zhang, F. 
  TITLE     Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery
  JOURNAL   Science
FEATURES             Location/Qualifiers
     source          1..6424
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(40..59)
                     /standard_name="SV40pro-F"
                     /label=SV40pro-F
     enhancer        161..540
     promoter        541..744
     primer_bind     695..715
                     /standard_name="CMV-F"
                     /label=CMV-F
     primer_bind     741..765
                     /standard_name="LNCX"
                     /label=LNCX
     intron          878..1353
                     /standard_name="-beta-globin intron"
                     /label=-beta-globin intron
     gene            1430..1531
                     /standard_name="PEG10"
                     /label=PEG10
     gene            1532..3656
                     /standard_name="PEG10"
                     /label=PEG10
     misc_feature    2584..3182
                     /standard_name="codon-swap"
                     /label=codon-swap
     misc_feature    3183
                     /standard_name="tile5"
                     /label=tile5
     misc_feature    complement(3657..3681)
                     /standard_name="HA"
                     /label=HA
     polyA_site      3766..4160
     primer_bind     complement(4499..4516)
                     /standard_name="L4440"
                     /label=L4440
     rep_origin      complement(4670..5258)
                     /direction=LEFT
     primer_bind     complement(4750..4769)
                     /standard_name="pBR322ori-F"
                     /label=pBR322ori-F
     CDS             complement(5429..6289)
                     /standard_name="AmpR"
                     /label=AmpR
     primer_bind     6052..6071
                     /standard_name="Amp-R"
                     /label=Amp-R
     promoter        complement(6290..6394)
ORIGIN
        1 ggatcccctg agggggcccc catgggctag aggatccggc ctcggcctct gcataaataa
       61 aaaaaattag tcagccatga gcttggccca ttgcatacgt tgtatccata tcataatatg
      121 tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt
      181 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      241 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      301 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      361 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      421 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      481 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      541 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      601 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      661 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      721 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      781 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt
      841 acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg
      901 ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag
      961 ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct
     1021 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc
     1081 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat
     1141 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg
     1201 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct
     1261 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta
     1321 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg
     1381 ctggcccatc actttggcaa agcacgtgag atctgaattc gccaccatgc tgggtcccga
     1441 ctgcccacct cctcctcctc cccctccccc caacaacaac aacaacaaca actccaagca
     1501 caccggccat aagagtgcgt gtgtccccaa catgaccgaa cgaagaaggg acgagctctc
     1561 tgaagagatc aacaacttaa gagagaaggt catgaagcag tcggaggaga acaacaacct
     1621 gcagagccag gtgcagaagc tcacagagga gaacaccacc cttcgagagc aagtggaacc
     1681 cacccctgag gatgaggatg atgacatcga gctccgcggt gctgcagcag ctgctgcccc
     1741 accccctcca atagaggaag agtgcccaga agacctccca gagaagttcg atggcaaccc
     1801 agacatgctg gctcctttca tggcccagtg ccagatcttc atggaaaaga gcaccaggga
     1861 tttctcagtt gatcgtgtcc gtgtctgctt cgtgacaagc atgatgaccg gccgtgctgc
     1921 ccgttgggcc tcagcaaagc tggagcgctc ccactacctg atgcacaact acccagcttt
     1981 catgatggaa atgaagcatg tctttgaaga ccctcagagg cgagaggttg ccaaacgcaa
     2041 gatcagacgc ctgcgccaag gcatggggtc tgtcatcgac tactccaatg ctttccagat
     2101 gattgcccag gacctggatt ggaacgagcc tgcgctgatt gaccagtacc acgagggcct
     2161 cagcgaccac attcaggagg agctctccca cctcgaggtc gccaagtcgc tgtctgctct
     2221 gattgggcag tgcattcaca ttgagagaag gctggccagg gctgctgcag ctcgcaagcc
     2281 acgctcgcca ccccgggcgc tggtgttgcc tcacattgca agccaccacc aggtagatcc
     2341 aaccgagccg gtgggaggtg cccgcatgcg cctgacgcag gaagaaaaag aaagacgcag
     2401 aaagctgaac ctgtgcctct actgtggaac aggaggtcac tacgctgaca attgtcctgc
     2461 caaggcctca aagtcttcgc cggcgggaaa ctccccggcc ccgctgtaga gggaccttca
     2521 gcgaccgggc cagaaataat aaggtcccca caagatgatg cctcatctcc acacttgcaa
     2581 gtgatgcttc agatacatct gccaggcagg cacacactct tcgtacgagc tatgattgat
     2641 tcaggggcat ctggtaactt catagatcac gagtatgtag ctcagaacgg aattcctttg
     2701 agaataaagg actggcccat cttggttgaa gctatcgatg ggaggcctat agcttccggt
     2761 cccgtggtgc atgaaacaca tgatcttatt gtcgatcttg gggatcaccg cgaagtcctc
     2821 tcatttgacg taacccagtc ccctttcttc cccgtggtcc tcggagtgcg gtggctgagt
     2881 actcacgatc ctaatattac atggagcacc cggtcaatcg tatttgatag tgagtactgt
     2941 aggtaccact gtcgaatgta tagtcctatt ccccctagtt tgcctccccc agctcctcaa
     3001 ccacccctgt actatcccgt ggacggatat agggtgtacc agcccgttcg atattactac
     3061 gtacaaaatg tttatacacc tgttgatgag cacgtctacc cagaccatag actcgtcgac
     3121 cctcacatcg aaatgattcc aggagcccat agtatacctt cagggcacgt atactctctg
     3181 tccgaacctg aaatggcagc tcttcgagat tttgtggcaa gaaatgtaaa agatgggcta
     3241 attactccaa cgattgcacc taatggagcc caagttctcc aggtgaagag ggggtggaaa
     3301 ctgcaagttt cttatgattg ccgagctcca aacaatttta ctatccagaa tcagtatcct
     3361 cgcctatcta ttccaaattt agaagaccaa gcacacctgg caacgtacac tgaattcgta
     3421 cctcaaatac ctggatacca aacatacccc acatatgccg cgtacccgac ctacccagta
     3481 ggattcgcct ggtacccagt gggacgagac ggacaaggaa gatcactata tgtacctgtg
     3541 atgatcactt ggaatccaca ctggtaccgc cagcctccgg taccacagta cccgccgcca
     3601 cagccgccgc ctccaccacc accaccgccg ccgcctccat cttacagtac cctgtatccg
     3661 tatgatgttc cggattatgc atgagaattc accccaccag tgcaggctgc ctatcagaaa
     3721 gtggtggctg gtgtggctaa tgccctggcc cacaagtatc actaagctcg ctttcttgct
     3781 gtccaatttc tattaaaggt tcctttgttc cctaagtcca actactaaac tgggggatat
     3841 tatgaagggc cttgagcatc tggattctgc ctaataaaaa acatttattt tcattgcaat
     3901 gatgtattta aattatttct gaatatttta ctaaaaaggg aatgtgggag gtcagtgcat
     3961 ttaaaacata aagaaatgaa gagctagttc aaaccttggg aaaatacact atatcttaaa
     4021 ctccatgaaa gaaggtgagg ctgcaaacag ctaatgcaca ttggcaacag cccctgatgc
     4081 ctatgcctta ttcatccctc agaaaaggat tcaagtagag gcttgatttg gaggttaaag
     4141 ttttgctatg ctgtatttta cattacttat tgttttagct gtcctcatga atgtcttttc
     4201 actacccatt tgcttatcct gcatctctca gccttgactc cactcagttc tcttgcttag
     4261 agataccacc tttcccctga agtgttcctt ccatgtttta cggcgagatg gtttctcctc
     4321 gcctggccac tcagccttag ttgtctctgt tgtcttatag aggtctactt gaagaaggaa
     4381 aaacaggggg catggtttga ctgtcctgtg agcccttctt ccctgcctcc cccactcaca
     4441 gtgacccgga atccctcgac atggcagtct agcactagtg cggccgcaga tctgcttcct
     4501 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4561 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4621 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4681 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4741 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4801 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4861 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4921 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4981 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5041 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5101 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5161 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5221 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5281 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     5341 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     5401 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     5461 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     5521 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     5581 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     5641 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     5701 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     5761 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     5821 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     5881 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     5941 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     6001 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     6061 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     6121 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     6181 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     6241 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     6301 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     6361 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     6421 acgt
//
