LOCUS       pCMV-SynA                6020 bp DNA     circular SYN 05-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...6ey-dp4tg70
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Segel, M., Lash, B., Song, J., Ladha, A., Liu, C.C., Jin, X., Mekhedov, S., Macrae, R.K., Koonin, E.V., Zhang, F. 
  TITLE     Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery
  JOURNAL   Science
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(40..59)
                     /standard_name="SV40pro-F"
                     /label=SV40pro-F
     enhancer        161..540
     promoter        541..744
     primer_bind     695..715
                     /standard_name="CMV-F"
                     /label=CMV-F
     misc_feature    698..716
                     /standard_name="pmd2.g_fwd"
                     /label=pmd2.g_fwd
                     /note="Geneious type: primer"
                     /note="sequence: aaatgggcggtaggcgtgt"
     primer_bind     741..765
                     /standard_name="LNCX"
                     /label=LNCX
     intron          878..1353
                     /standard_name="-beta-globin intron"
                     /label=-beta-globin intron
     gene            1427..3280
                     /standard_name="Syna"
                     /label=Syna
     polyA_site      3362..3756
     primer_bind     complement(4095..4112)
                     /standard_name="L4440"
                     /label=L4440
     rep_origin      complement(4266..4854)
                     /direction=LEFT
     primer_bind     complement(4346..4365)
                     /standard_name="pBR322ori-F"
                     /label=pBR322ori-F
     CDS             complement(5025..5885)
                     /standard_name="AmpR"
                     /label=AmpR
     primer_bind     5648..5667
                     /standard_name="Amp-R"
                     /label=Amp-R
     promoter        complement(5886..5990)
ORIGIN
        1 ggatcccctg agggggcccc catgggctag aggatccggc ctcggcctct gcataaataa
       61 aaaaaattag tcagccatga gcttggccca ttgcatacgt tgtatccata tcataatatg
      121 tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt
      181 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      241 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      301 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      361 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      421 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      481 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      541 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      601 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      661 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      721 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      781 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt
      841 acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg
      901 ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag
      961 ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct
     1021 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc
     1081 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat
     1141 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg
     1201 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct
     1261 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta
     1321 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg
     1381 ctggcccatc actttggcaa agcacgtgag atctgaattc gccaccatgg ttcgtccttg
     1441 ggttttctgt ctcctcctgt tcccttgttc ctctgcctac tcggacagct ggatgcccct
     1501 ggtaaacctc actcaacacc tcctccagga ggctaactct tccttctctt ccaactgctg
     1561 ggtctgctta tccatccaaa cccagcgctc tctagccatg ccagccccac taaggacttg
     1621 gacagagaca cccatgaaac ttcgaatcat gtactcagcc cggaccctct ccggcccata
     1681 ccctatcacc gaccttgaga ggcgcctcca gaatttccaa ccattgactc cccactcctc
     1741 ttttgtcaac cctgaccagc gggccattgc tttccttcag atcaccagcg tgacaggcat
     1801 acttcccata ctttctcgga tcacctcggt gagatacccc gatgaccacg tctatgaatc
     1861 tgcccagcgc cccatatggg gctcactctc cacccagacg atcctcacct cccaggcccc
     1921 tctctgcata tcccgcttct tcaagaattc aaaccatgcc accttcgtgg gcaaactccc
     1981 tgcctctctt tgcaatcaca cctttcagct ctccccctct gccaaccacc aatccataga
     2041 tctgtcctcc agctatgcat tcgccccatt aatggccatg ccagggtcta aatggagaaa
     2101 ccccttacgc ttttcaggac ccccttccct gaactcaggg atgcctcact actcctgccc
     2161 gatagatgac atccactgcc acacctaccc caccaccccc tggaggtcct gtccttcctt
     2221 cccagctagc acctgctata atctcaccct attcgagccg gacaattcga gccaccctat
     2281 taccctgtct gtggacacca catacttcaa gattaaactc cagggacaca aagaccccta
     2341 tccactcttt cagtaccagc ccctcatggg ggcagccctc tctggacaat attcaatctg
     2401 ggaatatgaa cccactgtta agaaaaacgg cggtatcact ccaaatatct tctcccacct
     2461 tgtctcctta acatactcct tctgcctcaa ctcctctggt gttttcttcc tctgtggaaa
     2521 ctcaacttat gtctgtctcc cggccaattg gtccggcgtc tgtacccttg tcttccaata
     2581 cccggatatt gaactccttc ccaacaacca aaccatatct gtcccgcttt ttgctacagt
     2641 tccctcctct gtccccgctt ctcgccggaa gcgagccctt cctctccttc ctctcctcgc
     2701 gggcctgggc attgcttctg ccctggggtt aggcatcgcg ggtatcacca cctcaactgt
     2761 gtatttccaa cagctttcca aggctctctc ggacagccta gatgaaatag ccacctccat
     2821 catcagcctc caagaccaaa tagactcgct ggcgggtgtc gttctccaaa accgcagagc
     2881 tctggacctc attgtggctg agaggggggg cacctgcctc ttcctccagg aagagtgctg
     2941 cttctacata aaccagtccg gggtagtccg gcacgcggca aggaaacttc gagaaagggc
     3001 ctcggaactc ggcacaagct cgagttcttg gatccagtgg ctggggctag gaccctggct
     3061 gccctcttgg ttgacttccc tcatggctcc cattctcttt atcctggtac tgctggtttt
     3121 caggccttgt cttcttaact gcctgactca ttctgtatcg cggcgaatga gttctttcat
     3181 tcacaccacc accgaaggac acgtggacaa gatccttctg cttcgagagt cccagtacaa
     3241 gagacttccc caagagcccc cggaggagga tgccgtctag gaattcaccc caccagtgca
     3301 ggctgcctat cagaaagtgg tggctggtgt ggctaatgcc ctggcccaca agtatcacta
     3361 agctcgcttt cttgctgtcc aatttctatt aaaggttcct ttgttcccta agtccaacta
     3421 ctaaactggg ggatattatg aagggccttg agcatctgga ttctgcctaa taaaaaacat
     3481 ttattttcat tgcaatgatg tatttaaatt atttctgaat attttactaa aaagggaatg
     3541 tgggaggtca gtgcatttaa aacataaaga aatgaagagc tagttcaaac cttgggaaaa
     3601 tacactatat cttaaactcc atgaaagaag gtgaggctgc aaacagctaa tgcacattgg
     3661 caacagcccc tgatgcctat gccttattca tccctcagaa aaggattcaa gtagaggctt
     3721 gatttggagg ttaaagtttt gctatgctgt attttacatt acttattgtt ttagctgtcc
     3781 tcatgaatgt cttttcacta cccatttgct tatcctgcat ctctcagcct tgactccact
     3841 cagttctctt gcttagagat accacctttc ccctgaagtg ttccttccat gttttacggc
     3901 gagatggttt ctcctcgcct ggccactcag ccttagttgt ctctgttgtc ttatagaggt
     3961 ctacttgaag aaggaaaaac agggggcatg gtttgactgt cctgtgagcc cttcttccct
     4021 gcctccccca ctcacagtga cccggaatcc ctcgacatgg cagtctagca ctagtgcggc
     4081 cgcagatctg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     4141 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     4201 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4261 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4321 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4381 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4441 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4501 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4561 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4621 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4681 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4741 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4801 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4861 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4921 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4981 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     5041 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     5101 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5161 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     5221 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5281 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     5341 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5401 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     5461 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5521 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5581 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5641 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5701 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5761 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     5821 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     5881 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5941 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6001 cgaaaagtgc cacctgacgt
//
