LOCUS       pCMV-SynB                6023 bp DNA     circular SYN 05-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...6f2-dp4ti6o
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Segel, M., Lash, B., Song, J., Ladha, A., Liu, C.C., Jin, X., Mekhedov, S., Macrae, R.K., Koonin, E.V., Zhang, F. 
  TITLE     Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery
  JOURNAL   Science
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(40..59)
                     /standard_name="SV40pro-F"
                     /label=SV40pro-F
     enhancer        161..540
     promoter        541..744
     primer_bind     695..715
                     /standard_name="CMV-F"
                     /label=CMV-F
     misc_feature    698..716
                     /standard_name="pmd2.g_fwd"
                     /label=pmd2.g_fwd
                     /note="Geneious type: primer"
                     /note="sequence: aaatgggcggtaggcgtgt"
     primer_bind     741..765
                     /standard_name="LNCX"
                     /label=LNCX
     intron          878..1353
                     /standard_name="-beta-globin intron"
                     /label=-beta-globin intron
     gene            1427..3283
                     /standard_name="Synb"
                     /label=Synb
     polyA_site      3365..3759
     primer_bind     complement(4098..4115)
                     /standard_name="L4440"
                     /label=L4440
     rep_origin      complement(4269..4857)
                     /direction=LEFT
     primer_bind     complement(4349..4368)
                     /standard_name="pBR322ori-F"
                     /label=pBR322ori-F
     CDS             complement(5028..5888)
                     /standard_name="AmpR"
                     /label=AmpR
     primer_bind     5651..5670
                     /standard_name="Amp-R"
                     /label=Amp-R
     promoter        complement(5889..5993)
ORIGIN
        1 ggatcccctg agggggcccc catgggctag aggatccggc ctcggcctct gcataaataa
       61 aaaaaattag tcagccatga gcttggccca ttgcatacgt tgtatccata tcataatatg
      121 tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt
      181 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      241 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      301 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      361 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      421 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      481 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      541 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      601 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      661 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      721 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      781 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt
      841 acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg
      901 ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag
      961 ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct
     1021 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc
     1081 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat
     1141 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg
     1201 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct
     1261 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta
     1321 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg
     1381 ctggcccatc actttggcaa agcacgtgag atctgaattc gccaccatga caggcttttg
     1441 ggtcctctgt ttcgtccttt tcccctcctc cttatcctat ccggaaagct ggatgcccct
     1501 tgtaaacctc actcaccaca tcctacgtga taccaactct tccctgtttt ccaactgttg
     1561 ggtctgcttg tctacccaaa cccagcggtc cttagcagtc ccagcccctc tgtccatttg
     1621 gacagataca cccatgaagc ttcatcttac ctactcagtc aggcccttct ctggctcctt
     1681 ttccattagc gacattgaaa gacgcctccg tctcttccgc ccactgactg cctcctattc
     1741 tttccacaat cctgacagaa gggcgattgc ttttcttcaa ctcgtcagct caacaggcat
     1801 atttcggatc atcacccgga taacctctgt gatatatccc cataaggacc gtttcttcga
     1861 atctgcccaa cgccctctct ggggaccact ctttactgag accgtgctca ggtcgcaggc
     1921 cccactctgc atatctcgct ttttcaaggt ctcagcatat gccacttttg taggcaacct
     1981 ctctgcctct ctctgcaact acaccatgca tatttcacct tctaccagtc atgaaaacct
     2041 agatctttcc accacccata cgttcaaaca ggcaatgaaa agaccggatg ccaaatggaa
     2101 aaacccgctc cgtttttccg ggcccccctc cctcatcttc tcgaagccgg cttactatcc
     2161 ctgcccaaca gacatcaaac actgccatac ctctccggcc actccctgga tgcactgtcc
     2221 tcaggctccc ttcggcacct gctataacct cactttattt gaaccagaca actcaaccca
     2281 ccctgttacc atgtcagtga accctaccca cttcaaggtc aaactccagg ggcacagaga
     2341 cccctatccg ctctcccatt accagcccct cacgggagct gccctgtctg gacaatattc
     2401 agtctgggag aacgagatca ctgtccaaga aaactgggac atcacctcca acattttctc
     2461 acatcttctc agcttctcgt acgccttctg cctcaactct tcaggcgttt tcttcctctg
     2521 cggaacatcg acttacatct gcctcccagc caattggtcc ggtgtctgta ccctggtctt
     2581 ccaatacccg gatattgaac ttctccccaa taaccaaacg gtgcctgttc ccctttttgc
     2641 ttcagttctt tcctcagact cagttcttcg cccaaagagg tcccctcacc tctttccctt
     2701 ccttgcaggc ctgggtatct cttctgccct tggtacgggg atagctggct tggccacctc
     2761 gactctctat ttccaacagc tttctaaggt tctttccgaa accttggaag aaatagctgc
     2821 ctctatcact accctccaga accaaataga ctcgctcgca ggtgttgttc tacaaaaccg
     2881 ccgagctctg gacctcatca ctgctgagaa agggggcacc tgtctcttcc tccaggaaga
     2941 gtgctgcttc tacgtaaacc agtctggaat agtccgggac gcggcaagga aactccaaga
     3001 acgagcatct gaactcggcc agcattctga ctcttgggga cagtggcctg accttggacg
     3061 ttggttgccc tggctgactc cctttctggg acctcttctc ttcctcttct tcctactgac
     3121 atttgggtct tgtcttctga actgcctaac ccgttttgtg tcccagagac ttggctcctt
     3181 tgttcaagac actgccaaaa ggcatgtgga cagcatcctc caaaatttcc aatataaaaa
     3241 actgccccaa gactccccag atgaggacac cattcctaca taagaattca ccccaccagt
     3301 gcaggctgcc tatcagaaag tggtggctgg tgtggctaat gccctggccc acaagtatca
     3361 ctaagctcgc tttcttgctg tccaatttct attaaaggtt cctttgttcc ctaagtccaa
     3421 ctactaaact gggggatatt atgaagggcc ttgagcatct ggattctgcc taataaaaaa
     3481 catttatttt cattgcaatg atgtatttaa attatttctg aatattttac taaaaaggga
     3541 atgtgggagg tcagtgcatt taaaacataa agaaatgaag agctagttca aaccttggga
     3601 aaatacacta tatcttaaac tccatgaaag aaggtgaggc tgcaaacagc taatgcacat
     3661 tggcaacagc ccctgatgcc tatgccttat tcatccctca gaaaaggatt caagtagagg
     3721 cttgatttgg aggttaaagt tttgctatgc tgtattttac attacttatt gttttagctg
     3781 tcctcatgaa tgtcttttca ctacccattt gcttatcctg catctctcag ccttgactcc
     3841 actcagttct cttgcttaga gataccacct ttcccctgaa gtgttccttc catgttttac
     3901 ggcgagatgg tttctcctcg cctggccact cagccttagt tgtctctgtt gtcttataga
     3961 ggtctacttg aagaaggaaa aacagggggc atggtttgac tgtcctgtga gcccttcttc
     4021 cctgcctccc ccactcacag tgacccggaa tccctcgaca tggcagtcta gcactagtgc
     4081 ggccgcagat ctgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     4141 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     4201 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4261 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4321 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4381 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4441 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     4501 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4561 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4621 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4681 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     4741 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4801 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4861 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4921 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     4981 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     5041 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     5101 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     5161 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     5221 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     5281 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     5341 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     5401 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     5461 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     5521 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     5581 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     5641 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     5701 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     5761 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     5821 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     5881 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     5941 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     6001 ccccgaaaag tgccacctga cgt
//
