LOCUS       pCMV-Mm_cargo(Cre)                7410 bp DNA     circular SYN 05-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...6dt-dp4sxo2
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Segel, M., Lash, B., Song, J., Ladha, A., Liu, C.C., Jin, X., Mekhedov, S., Macrae, R.K., Koonin, E.V., Zhang, F. 
  TITLE     Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery
  JOURNAL   Science
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        236..852
     regulatory      315..602
                     /standard_name="CAG_enhancer"
                     /label=CAG_enhancer
     misc_feature    769..789
                     /standard_name="CMV_fwd_primer"
                     /label=CMV_fwd_primer
                     /note="Geneious type: primer"
     promoter        863..881
     gene            929..1298
                     /standard_name="Peg10"
                     /label=Peg10
     exon            929..1201
                     /standard_name="Peg10-001 Exon 1"
                     /label=Peg10-001 Exon 1
     exon            1202..1298
                     /standard_name="Peg10-001 Exon 2"
                     /label=Peg10-001 Exon 2
     CDS             1279..1298
                     /standard_name="Translation 1279-1298"
                     /label=Translation 1279-1298
     CDS             1302..2327
                     /standard_name="Cre"
                     /label=Cre
     exon            2328..2889
                     /standard_name="Peg10-001 Exon 2"
                     /label=Peg10-001 Exon 2
     gene            2328..2889
                     /standard_name="Peg10"
                     /label=Peg10
     CDS             2328..2347
                     /standard_name="Translation 2328-2347"
                     /label=Translation 2328-2347
     misc_feature    complement(3004..3021)
                     /standard_name="BGH_rev_primer"
                     /label=BGH_rev_primer
                     /note="Geneious type: primer"
     terminator      3007..3234
     misc_feature    complement(3717..3737)
                     /standard_name="pBABE_3_primer"
                     /label=pBABE_3_primer
                     /note="Geneious type: primer"
     regulatory      complement(3723..3938)
                     /standard_name="SV40_enhancer"
                     /label=SV40_enhancer
     promoter        3735..4003
     misc_feature    3964..3983
                     /standard_name="SV40pro_F_primer"
                     /label=SV40pro_F_primer
                     /note="Geneious type: primer"
     gene            4121..4909
                     /standard_name="NeoR/KanR"
                     /label=NeoR/KanR
     terminator      5089..5208
     misc_feature    5177..5196
                     /standard_name="EBV_rev_primer"
                     /label=EBV_rev_primer
                     /note="Geneious type: primer"
     promoter        complement(5252..5270)
     misc_feature    complement(5269..5291)
                     /standard_name="M13_pUC_rev_primer"
                     /label=M13_pUC_rev_primer
                     /note="Geneious type: primer"
     promoter        complement(5305..5334)
     gene            complement(6414..7274)
                     /standard_name="Ampicillin"
                     /label=Ampicillin
     promoter        complement(7316..7344)
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagataata cgactcacta
      901 tagcccaagc tggctagcgt ttaaaaccgg atttggcgcc ccccctccta ggatctctct
      961 atataaggca agcagtccgg actcccgata cacgcgtttc caacttgagt tggtgtgtgt
     1021 cgaagaatcc tgaccaacta cgacctgggg agagcagcca accgagaagg tccaccgagc
     1081 ctcgcctagg tctgctgcgc gggctgcggt ccggagcctt ctccggaccg cggtcaccca
     1141 gtggaccggg ccgtcgcggg acccctcatc cttcgtggca tcgcagagga atcctcgtgt
     1201 ggaacaggcg ggttttaaga accaaaagac gccaaccacg agggtcccag gatccagggc
     1261 tccctcccca ggggagtgaa gcccctctca ccgcagccat gaatttactg accgtacacc
     1321 aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg
     1381 acatgttcag ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt
     1441 gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg
     1501 aagatgttcg cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc
     1561 agcaacattt gggccagcta aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg
     1621 acagcaatgc tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg
     1681 aacgtgcaaa acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca
     1741 tggaaaatag cgatcgctgc caggatatac gtaatctggc atttctgggg attgcttata
     1801 acaccctgtt acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg
     1861 acggtgggag aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg
     1921 tagagaaggc acttagcctg ggggtaacta aactggtcga gcgatggatt tccgtctctg
     1981 gtgtagctga tgatccgaat aactacctgt tttgccgggt cagaaaaaat ggtgttgccg
     2041 cgccatctgc caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc
     2101 atcgattgat ttacggcgct aaggatgact ctggtcagag atacctggcc tggtctggac
     2161 acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga
     2221 tcatgcaagc tggtggctgg accaatgtaa atattgtcat gaactatatc cgtaacctgg
     2281 atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg cgattagcat cctgcagtgc
     2341 tgcgtagaac ctgtcatgtc ctttgtagtc tctgccctca acttgatcct gtgcagcttc
     2401 tcaatctatg actgtgtggt actggacctt cagaggcgca cagagctcaa gtcagttttc
     2461 gtcttgactg ccactttata agttgacagg cctgggtttt acttgttaaa acctctcacc
     2521 atctcaatca caggctgcca agtgtcttta caaagaagct gatacaaaca caggccatgc
     2581 tgatttctta cagagggaga gaagaggaag agaagaagaa agaggaggaa gaggacatga
     2641 cttgcccata tgctgggcac cttataaagg aagccagact tttcggtgca gtatggaaag
     2701 gcttccgtga ttctcttgct gcaccccacg aaacttcacc accttcaaac tccattttca
     2761 cggttccgtt aattttcaag gagcagcaac tcgactggtt ctctgctaca tgaaacacct
     2821 cagcttgaaa aggaagtgct ctctcagact gacttgtgag tgtgccttca cattctggtg
     2881 caaatcatgc ttaagcttgg taccgagctc ggatccacta gtccagtgtg gtggaattct
     2941 gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgtttaa acccgctgat
     3001 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     3061 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     3121 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     3181 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     3241 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     3301 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     3361 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     3421 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     3481 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     3541 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     3601 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     3661 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     3721 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3781 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     3841 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     3901 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     3961 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     4021 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     4081 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     4141 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     4201 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     4261 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     4321 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     4381 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     4441 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     4501 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     4561 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     4621 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     4681 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     4741 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     4801 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     4861 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     4921 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     4981 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     5041 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     5101 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     5161 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     5221 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     5281 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     5341 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     5401 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     5461 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5521 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5581 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5641 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5701 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5761 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5821 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5881 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5941 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6001 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6061 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     6121 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6181 accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6241 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6301 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6361 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6421 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6481 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6541 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6601 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     6661 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     6721 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     6781 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     6841 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     6901 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     6961 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7021 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7081 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7141 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7201 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7261 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7321 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7381 acatttcccc gaaaagtgcc acctgacgtc
//
