LOCUS pCMV-Hs.cargo(Cre) 7505 bp DNA circular SYN 05-AUG-2021 DEFINITION synthetic circular DNA ACCESSION urn.local...6d3-dp4sijw VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10649) AUTHORS Segel, M., Lash, B., Song, J., Ladha, A., Liu, C.C., Jin, X., Mekhedov, S., Macrae, R.K., Koonin, E.V., Zhang, F. TITLE Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery JOURNAL Science FEATURES Location/Qualifiers source 1..7505 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 236..852 regulatory 315..602 /standard_name="CAG_enhancer" /label=CAG_enhancer promoter 863..881 gene 929..1407 /standard_name="PEG10" /label=PEG10 exon 929..1184 /standard_name="PEG10-001 Exon 1" /label=PEG10-001 Exon 1 exon 1185..1407 /standard_name="PEG10-001 Exon 2" /label=PEG10-001 Exon 2 CDS 1420..1440 /standard_name="SV40 NLS" /label=SV40 NLS CDS 1444..2467 /standard_name="Cre" /label=Cre gene 2470..2984 /standard_name="PEG10" /label=PEG10 terminator 3102..3329 misc_feature complement(3812..3832) /standard_name="pBABE_3_primer" /label=pBABE_3_primer /note="Geneious type: primer" regulatory complement(3818..4033) /standard_name="SV40_enhancer" /label=SV40_enhancer promoter 3830..4098 misc_feature 4059..4078 /standard_name="SV40pro_F_primer" /label=SV40pro_F_primer /note="Geneious type: primer" gene 4216..5004 /standard_name="NeoR/KanR" /label=NeoR/KanR terminator 5184..5303 misc_feature 5272..5291 /standard_name="EBV_rev_primer" /label=EBV_rev_primer /note="Geneious type: primer" promoter complement(5347..5365) misc_feature complement(5364..5386) /standard_name="M13_pUC_rev_primer" /label=M13_pUC_rev_primer /note="Geneious type: primer" promoter complement(5400..5429) gene complement(6509..7369) /standard_name="Ampicillin" /label=Ampicillin promoter complement(7411..7439) ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagataata cgactcacta 901 tagcccaagc tggctagcgt ttaaaaccct cctcggtgca acctatataa ggctcacagt 961 ctgcgctcct ggtacacgcg cttcaacttc ggttggtgtg tgtcgaagaa acctgactgc 1021 gccctgagga gaacagcgga gaaggtccac cgagcctggc gaaaggtccg ctgagcgggc 1081 tgtcgtccgg agccactccg ggctgcggag cacccagtgg agaccgcgcc tggctcaggt 1141 gtgggacccc atccttcctg tcttcgcaga ggagtcctcg cgtgaaataa gcgggttttg 1201 aaaacaaaaa aaagaaggag tggaagaggg ggccaggatc caggcctcca tccccacaga 1261 agtgaagcta cagctgggag gtctcctccc accccaaccg tcaccctggg tcccgactgc 1321 ccacctcctc ctcctccccc tccccccaac aacaacaaca acaacaactc caagcacacc 1381 ggccataaga gtgcgtgtgt ccccaacgcc accatgggcc caaagaagaa gagaaaggtt 1441 tcgaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat 1501 gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc 1561 tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg 1621 aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc 1681 ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg 1741 tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga 1801 aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat 1861 ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg 1921 gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg 1981 gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa 2041 acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc 2101 gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg 2161 gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg 2221 gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag 2281 agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc 2341 gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc 2401 atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat 2461 ggcgattaaa tacctgtcat gtccttcagg atctctgccc tcaaaattta ttcctgttca 2521 gcttctcaat cagtgactgt gtgctaaatt ttaggctact gtatcttcag gccacctgag 2581 gcacatcctc tctgaaacgg ctatggaagg ttagggccac tctggactgg cacacatcct 2641 aaagcaccaa aagaccttca acattttctg agagcaacag agtatttgcc aataaatgat 2701 ctctcatttt tccaccttga ctgccaatct aactaaaata attaataagt ttactttcca 2761 gccagtcctg gaagtctggg ttttacctgc caaaacctcc atcaccatct aaattatagg 2821 ctgccaaatt tgctgtttaa catttacaga gaagctgata caaacgcagg aaatgctgat 2881 ttctttatgg agggggagac gaggaggagg aggacatgac ttttcttgcg gtttcggtac 2941 cctcttttta aatcactgga ggactgaggc cttattaagg aagccttaag cttggtaccg 3001 agctcggatc cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc 3061 gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg 3121 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc 3181 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc 3241 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag 3301 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc 3361 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 3421 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 3481 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 3541 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 3601 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 3661 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 3721 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 3781 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag 3841 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 3901 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 3961 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 4021 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 4081 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 4141 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga 4201 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg 4261 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg 4321 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc 4381 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct 4441 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa 4501 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg 4561 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa 4621 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat 4681 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg 4741 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc 4801 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac 4861 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg 4921 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc 4981 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag 5041 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg 5101 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc 5161 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca 5221 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 5281 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg 5341 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 5401 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 5461 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 5521 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 5581 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 5641 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 5701 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 5761 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 5821 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 5881 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 5941 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 6001 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 6061 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 6121 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 6181 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 6241 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt 6301 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 6361 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 6421 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 6481 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 6541 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 6601 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 6661 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 6721 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 6781 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 6841 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 6901 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 6961 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 7021 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 7081 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 7141 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 7201 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 7261 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 7321 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 7381 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 7441 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 7501 acgtc //