LOCUS       pCMV-Hs.cargo(Cre)                7505 bp DNA     circular SYN 05-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...6d3-dp4sijw
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Segel, M., Lash, B., Song, J., Ladha, A., Liu, C.C., Jin, X., Mekhedov, S., Macrae, R.K., Koonin, E.V., Zhang, F. 
  TITLE     Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery
  JOURNAL   Science
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        236..852
     regulatory      315..602
                     /standard_name="CAG_enhancer"
                     /label=CAG_enhancer
     promoter        863..881
     gene            929..1407
                     /standard_name="PEG10"
                     /label=PEG10
     exon            929..1184
                     /standard_name="PEG10-001 Exon 1"
                     /label=PEG10-001 Exon 1
     exon            1185..1407
                     /standard_name="PEG10-001 Exon 2"
                     /label=PEG10-001 Exon 2
     CDS             1420..1440
                     /standard_name="SV40 NLS"
                     /label=SV40 NLS
     CDS             1444..2467
                     /standard_name="Cre"
                     /label=Cre
     gene            2470..2984
                     /standard_name="PEG10"
                     /label=PEG10
     terminator      3102..3329
     misc_feature    complement(3812..3832)
                     /standard_name="pBABE_3_primer"
                     /label=pBABE_3_primer
                     /note="Geneious type: primer"
     regulatory      complement(3818..4033)
                     /standard_name="SV40_enhancer"
                     /label=SV40_enhancer
     promoter        3830..4098
     misc_feature    4059..4078
                     /standard_name="SV40pro_F_primer"
                     /label=SV40pro_F_primer
                     /note="Geneious type: primer"
     gene            4216..5004
                     /standard_name="NeoR/KanR"
                     /label=NeoR/KanR
     terminator      5184..5303
     misc_feature    5272..5291
                     /standard_name="EBV_rev_primer"
                     /label=EBV_rev_primer
                     /note="Geneious type: primer"
     promoter        complement(5347..5365)
     misc_feature    complement(5364..5386)
                     /standard_name="M13_pUC_rev_primer"
                     /label=M13_pUC_rev_primer
                     /note="Geneious type: primer"
     promoter        complement(5400..5429)
     gene            complement(6509..7369)
                     /standard_name="Ampicillin"
                     /label=Ampicillin
     promoter        complement(7411..7439)
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagataata cgactcacta
      901 tagcccaagc tggctagcgt ttaaaaccct cctcggtgca acctatataa ggctcacagt
      961 ctgcgctcct ggtacacgcg cttcaacttc ggttggtgtg tgtcgaagaa acctgactgc
     1021 gccctgagga gaacagcgga gaaggtccac cgagcctggc gaaaggtccg ctgagcgggc
     1081 tgtcgtccgg agccactccg ggctgcggag cacccagtgg agaccgcgcc tggctcaggt
     1141 gtgggacccc atccttcctg tcttcgcaga ggagtcctcg cgtgaaataa gcgggttttg
     1201 aaaacaaaaa aaagaaggag tggaagaggg ggccaggatc caggcctcca tccccacaga
     1261 agtgaagcta cagctgggag gtctcctccc accccaaccg tcaccctggg tcccgactgc
     1321 ccacctcctc ctcctccccc tccccccaac aacaacaaca acaacaactc caagcacacc
     1381 ggccataaga gtgcgtgtgt ccccaacgcc accatgggcc caaagaagaa gagaaaggtt
     1441 tcgaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat
     1501 gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc
     1561 tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg
     1621 aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc
     1681 ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg
     1741 tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga
     1801 aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat
     1861 ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg
     1921 gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg
     1981 gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa
     2041 acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc
     2101 gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg
     2161 gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg
     2221 gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag
     2281 agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc
     2341 gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc
     2401 atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat
     2461 ggcgattaaa tacctgtcat gtccttcagg atctctgccc tcaaaattta ttcctgttca
     2521 gcttctcaat cagtgactgt gtgctaaatt ttaggctact gtatcttcag gccacctgag
     2581 gcacatcctc tctgaaacgg ctatggaagg ttagggccac tctggactgg cacacatcct
     2641 aaagcaccaa aagaccttca acattttctg agagcaacag agtatttgcc aataaatgat
     2701 ctctcatttt tccaccttga ctgccaatct aactaaaata attaataagt ttactttcca
     2761 gccagtcctg gaagtctggg ttttacctgc caaaacctcc atcaccatct aaattatagg
     2821 ctgccaaatt tgctgtttaa catttacaga gaagctgata caaacgcagg aaatgctgat
     2881 ttctttatgg agggggagac gaggaggagg aggacatgac ttttcttgcg gtttcggtac
     2941 cctcttttta aatcactgga ggactgaggc cttattaagg aagccttaag cttggtaccg
     3001 agctcggatc cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc
     3061 gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     3121 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     3181 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     3241 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     3301 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     3361 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3421 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     3481 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     3541 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     3601 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     3661 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     3721 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     3781 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     3841 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     3901 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3961 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4021 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4081 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4141 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     4201 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     4261 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     4321 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     4381 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     4441 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     4501 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     4561 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     4621 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     4681 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     4741 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     4801 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     4861 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     4921 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     4981 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     5041 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     5101 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     5161 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     5221 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5281 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     5341 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     5401 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     5461 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     5521 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     5581 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     5641 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     5701 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     5761 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5821 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5881 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5941 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     6001 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     6061 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     6121 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     6181 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6241 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt
     6301 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6361 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6421 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6481 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6541 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6601 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6661 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6721 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6781 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     6841 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     6901 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     6961 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7021 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7081 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7141 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7201 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7261 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7321 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7381 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7441 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7501 acgtc
//
