LOCUS       pLR5-CAG-TetR_W43F-3xm 10404 bp ds-DNA     circular     08-AUG-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      13..453
                     /label="F1 ori"
                     /ApEinfo_revcolor=#4682b4
                     /ApEinfo_fwdcolor=#4682b4
     rep_origin      complement(135..441)
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(460..528)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          593..616
                     /label="190129-M13F-BamHI-58I6"
                     /note="sequence: CGGTAGAATTGGATCCCGACGTTGTAAAACGACGGCCAGT"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          595..616
                     /label="170612-M13-F-46B9"
                     /note="sequence: ACGTTGTAAAACGACGGCCAGT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          595..616
                     /label="190810-M13-F-62I7"
                     /note="sequence: ACGTTGTAAAACGACGGCCAGT"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     599..616
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#008b8b
                     /ApEinfo_fwdcolor=#008b8b
     primer_bind     625..645
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(625..650)
                     /label="180528-pBSK-R-53B2"
                     /note="sequence: ATTCGCCCTATAGTGAGTCGTATTAC"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     promoter        626..643
                     /label="T7_promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     626..645
                     /label="T7"
                     /ApEinfo_revcolor=#008b8b
                     /ApEinfo_fwdcolor=#008b8b
     primer          complement(628..654)
                     /label="190315-pBSK-R-60D6"
                     /note="sequence: ACAACTTGTCTTCGTTCGGTACCCAATTCGCCCTATAGTGAGTCGTAT"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(628..654)
                     /label="180801-pBSK-R-53F7"
                     /note="sequence: ACAGGTCTTCTCGAAGTACCCAATTCGCCCTATAGTGAGTCGTAT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(635..654)
                     /label="170610-pBSK-R-45H8"
                     /note="sequence: GGGTCCGGGATGCAGAGTTCGGACACGTCCATGGTACCCAATTCGCCCTATAGTGA"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(635..654)
                     /label="170610-pBSK-R-45G9"
                     /note="sequence: ATCGATGGCTCGTCTCACACGGGTACCCAATTCGCCCTATAGTGA"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(635..654)
                     /label="170610-pBSK-R-46A4"
                     /note="sequence: ATCGATGGCTCGTCTCATCACGGTACCCAATTCGCCCTATAGTGA"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(635..654)
                     /label="170611-pBSK-R-45I6"
                     /note="sequence: TGCCATCTTTATCTCGGTACCCAATTCGCCCTATAGTGA"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     misc_feature    670..983
                     /label="piggyBac 5 terminal repeat"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     promoter        1054..1357
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(1153..1176)
                     /label="180617-CAG-seqR-53C8"
                     /note="sequence: CATTGACGTCAATGGAAAGTCCCT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     enhancer        1340..1638
                     /label="chicken beta actin promoter_regulatory"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(1608..1633)
                     /label="170101-mPax-R-39B9"
                     /note="sequence: ATCGATTTAACGTCTCACAAGCCGCCGCGCGCTTCGCTTTTTATAGG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     promoter        1609..2701
                     /label="CAG_promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Promoter        1609..2701
                     /label="CAG promoter (pCAGGS)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1698..1721
                     /label="190323-CAG-seqF2-60H5"
                     /note="sequence: CCCGGCTCTGACTGACCGCGTTAC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          2655..2680
                     /label="191009-CAG-seqF-64E7"
                     /note="sequence: TCCTGGGCAACGTGCTGGTTATTGTG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2724..2747
                     /label="180329-pLR-seqF-51C5"
                     /note="sequence: ACCCACTGCTTACTGGCTTATCGA"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     promoter        2751..2768
                     /label="T7_promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     2751..2770
                     /label="T7"
                     /ApEinfo_revcolor=#008b8b
                     /ApEinfo_fwdcolor=#008b8b
     primer          complement(2789..2814)
                     /label="181015-TetON-R-56D8"
                     /note="sequence: GATATCGCTAGCGACGATCCGAGGTC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(2791..2817)
                     /label="170102-MiR-R-39D3"
                     /note="sequence: ATCATTAGCTCGTCTCATCATGATATCGCTAGCGACGATCCGAGG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(2791..2818)
                     /label="170109-MiRLN3-R-41B9"
                     /note="sequence: ATCGATCTATCGTCTCACCATGATATCGCTAGCGACGATCCGAGG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(2791..2819)
                     /label="170302-invPCR-R-44C7"
                     /note="sequence: CACCGCCGTCCTTGCCCTTGGACTTGATGCACCCCATGATATCGCTAGCGACGATCCGAGG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(2791..2819)
                     /label="170102-Lyn-R-39C6"
                     /note="sequence: TCATTAGCTCGTCTCAGTCCTTGCCCTTGGACTTGATGCACCCCATGATATCGCTAGCGACGATCCGAGG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(2794..2818)
                     /label="170206-mRuby2-R-43A3"
                     /note="sequence: ATCGGATCGTGAATTCCATGATATCGCTAGCGACGATCCG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(2795..2818)
                     /label="170110-IRES-P-R-41C9"
                     /note="sequence: ATCGATCCGTCTCAATCCTTGTAGTCTCCGTCGTGGTCCTTATAGTCCATGATATCGCTAGCGACGATCC"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(2795..2818)
                     /label="170110-IRES-hyg-R-41D4"
                     /note="sequence: ATCGATCGTACGTCTCACCATGATATCGCTAGCGACGATCC"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(2795..2818)
                     /label="170209-IRES-P-R-44A2"
                     /note="sequence: ATCCTTGTAGTCTCCGTCGTGGTCCTTATAGTCCATGATATCGCTAGCGACGATCC"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(2812..2841)
                     /label="190223-NLS-anti-60A3"
                     /note="sequence: AATTGAACTTTTCTTTTCTTTTTTGGGCCCATGAT"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             2821..2841
                     /label="NLS"
                     /ApEinfo_revcolor=#086e01
                     /ApEinfo_fwdcolor=#086e01
     misc_feature    2821..2844
                     /label="SV40 NLS1"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     CDS             2845..3462
                     /label="TetR"
                     /ApEinfo_revcolor=#0d02fd
                     /ApEinfo_fwdcolor=#0d02fd
     misc_feature    2920..2973
                     /label="HTH motif_Baumeister et al 1992"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2968..2970
                     /label="W43F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             3493..4197
                     /label="mNeonGreen"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             4288..4992
                     /label="mNeonGreen"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             5074..5778
                     /label="mNeonGreen"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(5779..5799)
                     /label="170109-tdiRFP670-R-41C3"
                     /note="sequence: ATCGATGCTAGGTCTCAAACCTCGAGTTTAAACGCGGATCC"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          5806..5829
                     /label="170209-Lam-F-44A3"
                     /note="sequence: TGCCCAGCCGCCTAATGAGCGGCCGCATAGATAACT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          5806..5829
                     /label="170110-Lam-F-41D1"
                     /note="sequence: ACTGATCAGGCGTCTCATAATGAGCGGCCGCATAGATAACT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          5806..5829
                     /label="180329-pLR5-mC-F-51C4"
                     /note="sequence: GGCCTGGACAGCACCTAATGAGCGGCCGCATAGATAACT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          5808..5832
                     /label="161230-FUGW-F-39B2"
                     /note="sequence: ATTGCTAGCTCGTCTCATGAGCGGCCGCATAGATAACTGAT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          5809..5829
                     /label="170109-PalMiRLN3-F-41B4"
                     /note="sequence: ATCGATCGATCGTCTCATAGTGAGCGGCCGCATAGATAACT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          5809..5832
                     /label="170206-mRuby2-F-43A4"
                     /note="sequence: ATCGGATCGTCTTAAGTGAGCGGCCGCATAGATAACTGAT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          5809..5834
                     /label="170209-NB2A-NICD-F-44A7"
                     /note="sequence: TGAAACCTGAAGGCGTCTAGTGAGCGGCCGCATAGATAACTGATCC"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          5818..5842
                     /label="181008-IRES-F-55F4 (56E4)"
                     /note="sequence: ATGCATGATCCGTCTCATGAGCGGCATAGATAACTGATCCAGTGTGCT"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          5818..5842
                     /label="180903-IRES-F-55A1"
                     /note="sequence: TTAATTAATGAGCGGCATAGATAACTGATCCAGTGTGCT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     intron          5844..6139
                     /label="synth int"
                     /ApEinfo_revcolor=#4682b4
                     /ApEinfo_fwdcolor=#4682b4
     primer          complement(5948..5971)
                     /label="180903-synint-seqF-55B1"
                     /note="sequence: CGCGGGCCAGGTGAATATCAAATC"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(6143..6166)
                     /label="180818-beforeIRES-R-53G2"
                     /note="sequence: CGGAATTGGCCGCCCTAGATGCAT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     misc_feature    6188..6770
                     /label="internal ribosome entry site IRES"
                     /ApEinfo_revcolor=#c05e00
                     /ApEinfo_fwdcolor=#c05e00
     primer          6602..6625
                     /label="180903-IRES-Rseq-55A9"
                     /note="sequence: GGGGCTGAAGGATGCCCAGAAGGT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(6763..6786)
                     /label="181008-IRES-R-55F5 (56E5)"
                     /note="sequence: ATGCATGATCCGTCTCAGCTGCAGGAATTATCCCGGGTTGT"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(6763..6790)
                     /label="180903-IRES-R-55A2"
                     /note="sequence: ATTGGCTGCAGGAATTATCCCGGGTTGT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          6782..6805
                     /label="190718-newNeoR-F-62E8"
                     /note="sequence: TGTTTCCCGGCCTAGGGCAGCCAATATGGGATCGGCCATT"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          6782..6805
                     /label="190728-NeoR-F-62G1"
                     /note="sequence: AAGTGGGCTCGCGACGCAGCCAATATGGGATCGGCCATT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          6782..6806
                     /label="190817-NN-F-63A3"
                     /note="sequence: CTAGGGCAGCCAATATGGGATCGGCCATTG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          6782..6813
                     /label="190816-NewNeoR-F-62I9"
                     /note="sequence: TGTTTCCCGGCCTAGGGCAGCCAATATGGGATCGGCCATTGAACAAGA"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             6782..7591
                     /label="NeoR (PH(3')-II family aminoglycoside O-phosphotransferase)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(6966..6992)
                     /label="190911-IntN-NN-R-63E7"
                     /note="sequence: CTGCCTCGTCCTGCAGTTCATTCAGGG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(7568..7591)
                     /label="190817-NN-R-63A4"
                     /note="sequence: TGCTCCTGCTGTACCGAAGAACTCGTCAAGAAGGCGATA"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(7568..7591)
                     /label="190728-NeoR-R-62G2"
                     /note="sequence: GAAGAACTCGTCAAGGAAGAACTCGTCAAGAAGGCGATA"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(7568..7591)
                     /label="190718-newNeoR-R-62E9"
                     /note="sequence: AGCTCCAGCAGTTCCGAAGAACTCGTCAAGAAGGCGATA"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(7572..7591)
                     /label="190803-NewNeoR-R-62I3"
                     /note="sequence: CTTTACAGCCCCAGCTCCAGCTCCAGCAGTTCCGAAGAACTCGTCAAGAAGGC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          7592..7616
                     /label="170417-TetON-F-44I5"
                     /note="sequence: TGAGGGGATCAATTCTCTAGATAAC"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          7724..7749
                     /label="170101-TKHyg-F-39B7"
                     /note="sequence: ATCGTATCAAGAAGACAACTTGTTTATTGCAGCTTATAATGG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          7754..7791
                     /label="191215-TsixFra1-F-66I2"
                     /note="sequence: GATGCCTGGGACTGGGAGACAAGCTTCTGACCTCTTCTCTTCCTCCCACAGGAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    7754..7804
                     /label="SV40 early mRNA polyadenylation signal"
                     /ApEinfo_revcolor=#727272
                     /ApEinfo_fwdcolor=#727272
     primer          7904..7929
                     /label="191019-PB-invF-65B3"
                     /note="sequence: AACCCCTATTTGCCCTCTGTATACCGTCGACATACTAGTTA"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          7904..7929
                     /label="191019-PB-invF-65B3"
                     /note="sequence: AACCCCTATTTGCCCTCTGTATACCGTCGACATACTAGTTA"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    7928..8169
                     /label="piggyBac 3 terminal repeat"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer          8202..8230
                     /label="180402-pBSK-BS-F-51D2"
                     /note="sequence: GATTTTTGAAATTTTGGAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          8207..8236
                     /label="180528-pBSK-F-53B3"
                     /note="sequence: CTTTTGTTCCCTTTAGTGAGGGTTAATTGC"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          8208..8231
                     /label="170610-pBSK-F-45H1"
                     /note="sequence: ATCGATGGCTCGTCTCACTGTTTTTGTTCCCTTTAGTGAGGGTTA"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          8208..8231
                     /label="170611-pBSK-F-45I7"
                     /note="sequence: GAATGGTCTTTCTGATTTTGTTCCCTTTAGTGAGGGTTA"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          8208..8231
                     /label="170610-pBSK-F-46A5"
                     /note="sequence: ATCGATGGCTCGTCTCACTGTTTTTGTTCCCTTTAGTGAGGGTTA"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          8208..8231
                     /label="170610-pBSK-F-45H9"
                     /note="sequence: AGAGTTGGTTGAAGGTTTTGTTCCCTTTAGTGAGGGTTA"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          8208..8237
                     /label="190315-pBSK-F-60D7"
                     /note="sequence: AAACATGTCTTCCTATTTTGTTCCCTTTAGTGAGGGTTAATTGCG"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          8208..8237
                     /label="180801-pBSK-F-53F8"
                     /note="sequence: AACACCGGGTCTTCGTTTTGTTCCCTTTAGTGAGGGTTAATTGCG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer_bind     complement(8214..8233)
                     /label="T3"
                     /ApEinfo_revcolor=#008b8b
                     /ApEinfo_fwdcolor=#008b8b
     promoter        complement(8216..8234)
                     /label="T3 promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     complement(8251..8271)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#008b8b
                     /ApEinfo_fwdcolor=#008b8b
     primer          complement(8255..8276)
                     /label="170612-M13-R-46C1"
                     /note="sequence: TCACACAGGAAACAGCTATGAC"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(8255..8276)
                     /label="190810-M13-R-62I6"
                     /note="sequence: TCACACAGGAAACAGCTATGAC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_binding    complement(8277..8299)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(8303..8333)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     Promoter        complement(8304..8333)
                     /label="lac"
                     /ApEinfo_revcolor=#4682b4
                     /ApEinfo_fwdcolor=#4682b4
     primer          complement(8325..8348)
                     /label="170828-tdmRuby2-R-47G9"
                     /note="sequence: TTAGGCACCCCAGGCTTTACACTT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          8334..8357
                     /label="170828-tdiRFP-F-47G7"
                     /note="sequence: GCCTGGGGTGCCTAATGAGTGAGC"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(8449..8472)
                     /label="181225-delta-sapI-pB-R-58H1"
                     /note="sequence: GCCCAATACGCAAACCGCCTCTCC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(8449..8475)
                     /label="181225-delta-Sap-R-58E2"
                     /note="sequence: GACCGAGCGCGCTCTTCAAGCGCCCAATACGCAAACCGCCTCTCC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          8480..8503
                     /label="181225-delta-sapI-pB-F-58G9"
                     /note="sequence: CGCTTCCTCGCTCACTGACTCGCT"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(8630..8653)
                     /label="190326-gRNA2-F-60H9"
                     /note="sequence: GCTATGCTGTTTTTTGAGGGGCCAGCAACGCGGCCTTTTTACGG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(8639..9267)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#4682b4
                     /ApEinfo_fwdcolor=#4682b4
     rep_origin      complement(8639..9321)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(8695..8718)
                     /label="190716-ColE1-seqF-62E4"
                     /note="sequence: GCCACCTCTGACTTGAGCGTCGAT"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          9315..9349
                     /label="181107-LWLP-F-56G8"
                     /note="sequence: GTCATGAGATTATCAAAAAGGATCTTCACCTAGAT"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          9419..9442
                     /label="171113-Amp-probe-F-48D7"
                     /note="sequence: CCAATGCTTAATCAGTGAGGCACC"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     CDS             complement(9419..10078)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(9419..10273)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          9843..9866
                     /label="191019-down-R-64H3"
                     /note="sequence: TCCTTCGGTCCTCCGATCGTTGTC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(10141..10164)
                     /label="171113-Amp-probe-R-48D8"
                     /note="sequence: TGGGTGCACGAGTGGGTTACATCG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     Promoter        complement(10318..10346)
                     /label="Amp prom"
                     /ApEinfo_revcolor=#4682b4
                     /ApEinfo_fwdcolor=#4682b4
ORIGIN
        1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
       61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
      121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC
      181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
      241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG
      301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA
      361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC
      421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCAT TCAGGCTGCG
      481 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG
      541 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG
      601 TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT TGGGGCGCGC
      661 CATTCTAGAT TAACCCTAGA AAGATAGTCT GCGTAAAATT GACGCATGCA TTCTTGAAAT
      721 ATTGCTCTCT CTTTCTAAAT AGCGCGAATC CGTCGCTGTG CATTTAGGAC ATCTCAGTCG
      781 CCGCTTGGAG CTCCCGTGAG GCGTGCTTGT CAATGCGGTA AGTGTCACTG ATTTTGAACT
      841 ATAACGACCG CGTGAGTCAA AATGACGCAT GATTATCTTT TACGTGACTT TTAAGATTTA
      901 ACTCATACGA TAATTATATT GTTATTTCAT GTTCTACTTA CGTGATAACT TATTATATAT
      961 ATATTTTCTT GTTATAGATA TCAACTAGAA TGCTAGTTAT TAATAGTAAT CAATTACGGG
     1021 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC
     1081 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT
     1141 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC
     1201 CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA
     1261 CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG
     1321 GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTC GAGGTGAGCC CCACGTTCTG
     1381 CTTCACTCTC CCCATCTCCC CCCCCTCCCC ACCCCCAATT TTGTATTTAT TTATTTTTTA
     1441 ATTATTTTGT GCAGCGATGG GGGCGGGGGG GGGGGGGGGG CCCCCCCCAG GCGGGGCGGG
     1501 GCGGGGCGAG GGGCGGGGCG GGGCGAGGCG GAAAGGTGCG GCGGCAGCCA ATCAGAGCGG
     1561 CGCGCTCCGA AAGTTTCCTT TTATGGCGAG GCGGCGGCGG CGGCGGCCCT ATAAAAAGCG
     1621 AAGCGCGCGG CGGGCGGGAG TCGCTGCGCG CTGCCTTCGC CCCGTGCCCC GCTCCGCCGC
     1681 CGCCTCGCGC CGCCCGCCCC GGCTCTGACT GACCGCGTTA CTCCCACAGG TGAGCGGGCG
     1741 GGACGGCCCT TCTCCTCCGG GCTGTAATTA GCGCTTGGTT TAATGACGGC TTGTTTCTTT
     1801 TCTGTGGCTG CGTGAAAGCC TTGAGGGGCT CCGGGAGGGC CCTTTGTGCG GGGGGAGCGG
     1861 CTCGGGGGGT GCGTGCGTGT GTGTGTGCGT GGGGAGCGCC GCGTGCGGCT CCGCGCTGCC
     1921 CGGCGGCTGT GAGCGCTGCG GGCGCGGCGC GGGGCTTTGT GCGCTCCGCA GTGTGCGCGA
     1981 GGGGAGCGCG GCCGGGGGCG GTGCCCCGCG GTGCGGGGGG GGCTGCGAGG GGAACAAAGG
     2041 CTGCGTGCGG GGTGTGTGCG TGGGGGGGTG AGCAGGGGGT GTGGGCGCGT CGGTCGGGCT
     2101 GCAACCCCCC CTGCACCCCC CTCCCCGAGT TGCTGAGCAC GGCCCGGCTT CGGGTGCGGG
     2161 GCTCCGTACG GGGCGTGGCG CGGGGCTCGC CGTGCCGGGC GGGGGGTGGC GGCAGGTGGG
     2221 GGTGCCGGGC GGGGCGGGGC CGCCTCGGGC CGGGGAGGGC TCGGGGGAGG GGCGCGGCGG
     2281 CCCCCGGAGC GCCGGCGGCT GTCGAGGCGC GGCGAGCCGC AGCCATTGCC TTTTATGGTA
     2341 ATCGTGCGAG AGGGCGCAGG GACTTCCTTT GTCCCAAATC TGTGCGGAGC CGAAATCTGG
     2401 GAGGCGCCGC CGCACCCCCT CTAGCGGGCG CGGGGCGAAG CGGTGCGGCG CCGGCAGGAA
     2461 GGAAATGGGC GGGGAGGGCC TTCGTGCGTC GCCGCGCCGC CGTCCCCTTC TCCCTCTCCA
     2521 GCCTCGGGGC TGTCCGCGGG GGGACGGCTG CCTTCGGGGG GGACGGGGCA GGGCGGGGTT
     2581 CGGCTTCTGG CGTGTGACCG GCGGCTCTAG AGCCTCTGCT AACCATGTTC ATGCCTTCTT
     2641 CTTTTTCCTA CAGCTCCTGG GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA
     2701 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
     2761 CACTATAGGG AGACCCAAGC TTGGTATCGA CCTCGGATCG TCGCTAGCGA TATCATGGGC
     2821 CCAAAAAAGA AAAGAAAAGT TGGCTCTAGA TTAGATAAAA GTAAAGTGAT TAACAGCGCA
     2881 TTAGAGCTGC TTAATGAGGT CGGAATCGAA GGTTTAACAA CCCGTAAACT CGCCCAGAAG
     2941 CTAGGTGTAG AGCAGCCTAC ATTGTATTTC CATGTAAAAA ATAAGCGGGC TTTGCTCGAC
     3001 GCCTTAGCCA TTGAGATGTT AGATAGGCAC CATACTCACT TTTGCCCTTT AGAAGGGGAA
     3061 AGCTGGCAAG ATTTTTTACG TAATAACGCT AAAAGTTTTA GATGTGCTTT ACTAAGTCAT
     3121 CGCGATGGAG CAAAAGTACA TTTAGGTACA CGGCCTACAG AAAAACAGTA TGAAACTCTC
     3181 GAAAATCAAT TAGCCTTTTT ATGCCAACAA GGTTTTTCAC TAGAGAATGC ATTATATGCA
     3241 CTCAGCGCTG TGGGGCATTT TACTTTAGGT TGCGTATTGG AAGATCAAGA GCATCAAGTC
     3301 GCTAAAGAAG AAAGGGAAAC ACCTACTACT GATAGTATGC CGCCATTATT ACGACAAGCT
     3361 ATCGAATTAT TTGATCACCA AGGTGCAGAG CCAGCCTTCT TATTCGGCCT TGAATTGATC
     3421 ATATGCGGAT TAGAAAAACA ACTTAAATGT GAAAGTGGGT CTCCGCTAGC CTCCAAAACC
     3481 GAATTCACCA TGGTGAGTAA GGGAGAGGAA GATAATATGG CCTCCCTTCC CGCTACGCAC
     3541 GAACTCCACA TCTTCGGGTC AATCAACGGT GTTGACTTCG ACATGGTGGG CCAGGGCACC
     3601 GGCAATCCCA ATGACGGATA CGAAGAACTC AATTTGAAGA GTACAAAGGG CGATCTCCAA
     3661 TTCTCACCTT GGATTCTGGT TCCCCACATT GGATACGGAT TTCATCAGTA CCTGCCGTAC
     3721 CCCGATGGGA TGAGCCCATT TCAGGCTGCA ATGGTAGATG GTAGCGGTTA CCAAGTACAC
     3781 CGAACTATGC AATTTGAGGA CGGTGCCTCA CTGACAGTGA ACTATCGGTA TACTTACGAA
     3841 GGAAGCCACA TCAAGGGAGA GGCACAGGTC AAAGGAACCG GATTTCCAGC CGACGGGCCA
     3901 GTCATGACAA ACTCCCTGAC CGCCGCAGAT TGGTGCCGCA GCAAAAAGAC CTATCCAAAT
     3961 GACAAGACCA TTATCTCGAC ATTCAAATGG AGCTACACCA CCGGAAACGG CAAACGCTAT
     4021 CGGTCTACCG CCAGGACAAC CTACACATTT GCAAAACCTA TGGCCGCAAA CTATCTGAAA
     4081 AACCAGCCGA TGTATGTGTT CCGAAAGACG GAATTAAAAC ACTCGAAAAC AGAACTAAAC
     4141 TTTAAAGAGT GGCAGAAAGC CTTTACCGAC GTAATGGGCA TGGACGAGCT GTATAAGCCT
     4201 GCAGGCGGGT CGAGGCCGGA CCCCGCGCTG GCCGCACTCG GAGGCGGCGG AAGCGGTGGC
     4261 GGAGGGTCAG GCGGAGGTGG ATCCATGGTG AGTAAGGGAG AGGAAGATAA TATGGCCTCC
     4321 CTTCCCGCTA CGCACGAACT CCACATCTTC GGGTCAATCA ACGGTGTTGA CTTCGACATG
     4381 GTGGGCCAGG GCACCGGCAA TCCCAATGAC GGATACGAAG AACTCAATTT GAAGAGTACA
     4441 AAGGGCGATC TCCAATTCTC ACCTTGGATT CTGGTTCCCC ACATTGGATA CGGATTTCAT
     4501 CAGTACCTGC CGTACCCCGA TGGGATGAGC CCATTTCAGG CTGCAATGGT AGATGGTAGC
     4561 GGTTACCAAG TACACCGAAC TATGCAATTT GAGGACGGTG CCTCACTGAC AGTGAACTAT
     4621 CGGTATACTT ACGAAGGAAG CCACATCAAG GGAGAGGCAC AGGTCAAAGG AACCGGATTT
     4681 CCAGCCGACG GGCCAGTCAT GACAAACTCC CTGACCGCCG CAGATTGGTG CCGCAGCAAA
     4741 AAGACCTATC CAAATGACAA GACCATTATC TCGACATTCA AATGGAGCTA CACCACCGGA
     4801 AACGGCAAAC GCTATCGGTC TACCGCCAGG ACAACCTACA CATTTGCAAA ACCTATGGCC
     4861 GCAAACTATC TGAAAAACCA GCCGATGTAT GTGTTCCGAA AGACGGAATT AAAACACTCG
     4921 AAAACAGAAC TAAACTTTAA AGAGTGGCAG AAAGCCTTTA CCGACGTAAT GGGCATGGAC
     4981 GAGCTGTATA AGCTCTCCAG GCCGGACCCC GCGCTGGCCG CACTCGGAGG CGGCGGAAGC
     5041 GGTGGCGGAG GGTCAGGCGG AGGTGGATCC ATGGTGAGTA AGGGAGAGGA AGATAATATG
     5101 GCCTCCCTTC CCGCTACGCA CGAACTCCAC ATCTTCGGGT CAATCAACGG TGTTGACTTC
     5161 GACATGGTGG GCCAGGGCAC CGGCAATCCC AATGACGGAT ACGAAGAACT CAATTTGAAG
     5221 AGTACAAAGG GCGATCTCCA ATTCTCACCT TGGATTCTGG TTCCCCACAT TGGATACGGA
     5281 TTTCATCAGT ACCTGCCGTA CCCCGATGGG ATGAGCCCAT TTCAGGCTGC AATGGTAGAT
     5341 GGTAGCGGTT ACCAAGTACA CCGAACTATG CAATTTGAGG ACGGTGCCTC ACTGACAGTG
     5401 AACTATCGGT ATACTTACGA AGGAAGCCAC ATCAAGGGAG AGGCACAGGT CAAAGGAACC
     5461 GGATTTCCAG CCGACGGGCC AGTCATGACA AACTCCCTGA CCGCCGCAGA TTGGTGCCGC
     5521 AGCAAAAAGA CCTATCCAAA TGACAAGACC ATTATCTCGA CATTCAAATG GAGCTACACC
     5581 ACCGGAAACG GCAAACGCTA TCGGTCTACC GCCAGGACAA CCTACACATT TGCAAAACCT
     5641 ATGGCCGCAA ACTATCTGAA AAACCAGCCG ATGTATGTGT TCCGAAAGAC GGAATTAAAA
     5701 CACTCGAAAA CAGAACTAAA CTTTAAAGAG TGGCAGAAAG CCTTTACCGA CGTAATGGGC
     5761 ATGGACGAGC TGTATAAGGG ATCCGCGTTT AAACTCGAGG TTAATTAATG AGCGGCCGCA
     5821 TAGATAACTG ATCCAGTGTG CTGGAATTAA TTCGCTGTCT GCGAGGGCCA GCTGTTGGGG
     5881 TGAGTACTCC CTCTCAAAAG CGGGCATGAC TTCTGCGCTA AGATTGTCAG TTTCCAAAAA
     5941 CGAGGAGGAT TTGATATTCA CCTGGCCCGC GGTGATGCCT TTGAGGGTGG CCGCGTCCAT
     6001 CTGGTCAGAA AAGACAATCT TTTTGTTGTC AAGCTTGAGG TGTGGCAGGC TTGAGATCTG
     6061 GCCATACACT TGAGTGACAA TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC
     6121 CAGGTCCAAC TGCAGGTCGA GCATGCATCT AGGGCGGCCA ATTCCGCCCC TCTCCCCCCC
     6181 CCCCTTTTCC CTCCCCCCCC CCTAACGTTA CTGGCCGAAG CCGCTTGGAA TAAGGCCGGT
     6241 GTGCGTTTGT CTATATGTTA TTTTCCACCA TATTGCCGTC TTTTGGCAAT GTGAGGGCCC
     6301 GGAAACCTGG CCCTGTCTTC TTGACGAGCA TTCCTAGGGG TCTTTCCCCT CTCGCCAAAG
     6361 GAATGCAAGG TCTGTTGAAT GTCGTGAAGG AAGCAGTTCC TCTGGAAGCT TCTTGAAGAC
     6421 AAACAACGTC TGTAGCGACC CTTTGCAGGC AGCGGAACCC CCCACCTGGC GACAGGTGCC
     6481 TCTGCGGCCA AAAGCCACGT GTATAAGATA CACCTGCAAA GGCGGCACAA CCCCAGTGCC
     6541 ACGTTGTGAG TTGGATAGTT GTGGAAAGAG TCAAATGGCT CTCCTCAAGC GTATTCAACA
     6601 AGGGGCTGAA GGATGCCCAG AAGGTACCCC ATTGTATGGG ATCTGATCTG GGGCCTCGGT
     6661 GCACATGCTT TACATGTGTT TAGTCGAGGT TAAAAAAACG TCTAGGCCCC CCGAACCACG
     6721 GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAAGCTTG CCACAACCCG GGATAATTCC
     6781 TGCAGCCAAT ATGGGATCGG CCATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC
     6841 TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC
     6901 CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC
     6961 CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG
     7021 CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT
     7081 GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC
     7141 CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA
     7201 CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA
     7261 TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT
     7321 CAAGGCGCGC ATGCCCGACG GCGATGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC
     7381 GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT
     7441 GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG
     7501 CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT
     7561 CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGGGGAT CAATTCTCTA GATAACTGAT
     7621 CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT
     7681 CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT TTATTGCAGC
     7741 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
     7801 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTAACGCG TCGAGTGCAT
     7861 TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACA
     7921 TACTAGTTAA AAGTTTTGTT ACTTTATAGA AGAAATTTTG AGTTTTTGTT TTTTTTTAAT
     7981 AAATAAATAA ACATAAATAA ATTGTTTGTT GAATTTATTA TTAGTATGTA AGTGTAAATA
     8041 TAATAAAACT TAATATCTAT TCAAATTAAT AAATAAACCT CGATATACAG ACCGATAAAA
     8101 CACATGCGTC AATTTTACGC ATGATTATCT TTAACGTACG TCACAATATG ATTATCTTTC
     8161 TAGGGTTAAT CTAGTATACG CGTATGCGGC CGCTTAATTA ATCCAGCTTT TGTTCCCTTT
     8221 AGTGAGGGTT AATTGCGCGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT
     8281 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG
     8341 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT
     8401 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT
     8461 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC
     8521 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG
     8581 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG
     8641 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC
     8701 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG
     8761 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT
     8821 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG
     8881 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT
     8941 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC
     9001 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT
     9061 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC
     9121 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA
     9181 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT
     9241 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC
     9301 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT
     9361 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC
     9421 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG
     9481 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG
     9541 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC
     9601 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA
     9661 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG
     9721 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT
     9781 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA
     9841 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG
     9901 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA
     9961 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT
    10021 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA
    10081 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT
    10141 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT
    10201 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA
    10261 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT
    10321 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC
    10381 GCACATTTCC CCGAAAAGTG CCAC
//