LOCUS       pTV-Nanog_2-5prime-100 10205 bp ds-DNA     circular     08-AUG-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          complement(8..55)
                     /label="200110-sv40pAE-R-67G9"
                     /note="sequence: CAAAACTTTTAACTAGTATGTCGGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTAC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      13..453
                     /label="F1 ori"
                     /ApEinfo_revcolor=#4682b4
                     /ApEinfo_fwdcolor=#4682b4
     rep_origin      complement(135..441)
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(460..528)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          593..616
                     /label="190129-M13F-BamHI-58I6"
                     /note="sequence: CGGTAGAATTGGATCCCGACGTTGTAAAACGACGGCCAGT"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          595..616
                     /label="200104-M13-F-67C1 (67C4)"
                     /note="sequence: ACGTTGTAAAACGACGGCCAGT"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          595..616
                     /label="170612-M13-F-46B9"
                     /note="sequence: ACGTTGTAAAACGACGGCCAGT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          595..616
                     /label="190810-M13-F-62I7"
                     /note="sequence: ACGTTGTAAAACGACGGCCAGT"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     599..616
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     625..638
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     625..645
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(625..650)
                     /label="180528-pBSK-R-53B2"
                     /note="sequence: ATTCGCCCTATAGTGAGTCGTATTAC"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer_bind     626..638
                     /label="T7"
                     /ApEinfo_revcolor=#008b8b
                     /ApEinfo_fwdcolor=#008b8b
     promoter        626..638
                     /label="T7_promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        626..643
                     /label="T7_promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     626..645
                     /label="T7"
                     /ApEinfo_revcolor=#008b8b
                     /ApEinfo_fwdcolor=#008b8b
     primer          complement(628..653)
                     /label="190315-pBSK-R-60D6"
                     /note="sequence: ACAACTTGTCTTCGTTCGGTACCCAATTCGCCCTATAGTGAGTCGTAT"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(628..653)
                     /label="180801-pBSK-R-53F7"
                     /note="sequence: ACAGGTCTTCTCGAAGTACCCAATTCGCCCTATAGTGAGTCGTAT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          639..701
                     /label="200128-Nanog-5HAF-68A9"
                     /note="sequence: TATAGGGCGAATTGGTTTGACATGCCTGCAAGTGCAGGGCATGGACCAACTTACTAAGGTAGC"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     motif           654..676
                     /label="target_seq_sgRNA_target"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     None            654..676
                     /label="Target_sequence"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          770..793
                     /label="200227-Nanog-L-F-69G9"
                     /note="sequence: TATAGGGCGAATTGGGTACGATTTCCCCAGGTTTCCCAATGTG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          967..1002
                     /label="190926-Nanog-5-500F-63F8"
                     /note="sequence: TATAGGGCGAATTGGTTTGACATGCCTGCAAGTGCAGGGTTAAAAGATGAATAAAGTGAAATGAGGTAAAGCC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          1047..1066
                     /label="190209-promoter-qPCR-F-59A2"
                     /note="sequence: TAAGCTTTCCCTCCCTCCCA"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(1180..1199)
                     /label="190209-promoter-qPCR-F-59A9"
                     /note="sequence: TACCCACCCCCTATTCTCCC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          1318..1343
                     /label="190926-Nanog-5-150F-63F9"
                     /note="sequence: TATAGGGCGAATTGGTTTGACATGCCTGCAAGTGCAGGGGCTCACTTCCTTCTGACTTCTTGAT"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          1417..1467
                     /label="190926-Nanog-5-51F-63G1"
                     /note="sequence: TATAGGGCGAATTGGTAAAACCTTTTCAGAAATCCCTTCCCTCGCCATCACACTGACATGAGTGTGTATAGGGCGAATTGGTTTGACATGCCTGCAAGTGCAGGTAAAACCTTTTCAGAAATCCCTTCCCTCGCCATCACACTGACATGAGTGTG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(1444..1467)
                     /label="190926-Nanog-5-R-63G2"
                     /note="sequence: CTTATCGCTTACCTGCACACTCATGTCAGTGTGATGGCG"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          1447..1466
                     /label="190926-Nanog-sg-F-64A3"
                     /note="sequence: CACCGCATCACACTGACATGAGTGT"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          1468..1491
                     /label="190926-Nanog-3-F-63G3"
                     /note="sequence: GCAAGCAATTGCTTCGGTCTTCCTGGTCCCCACAGTTTG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(1468..1518)
                     /label="190926-Nanog-3-51F-63G7"
                     /note="sequence: GGGAACAAAAGCTGGTTTGACATGCCTGCAAGTGCAGGAGAATTCGATGCTTCCTCAGAACTAGGCAAACTGTGGGGACCAGGAAGACC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(1530..1569)
                     /label="200128-Nanog-5HAR-68B1"
                     /note="sequence: CTTATCGCTTACCTGGGGATGAAAAACTGCAGGCATTGAT"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          1554..1578
                     /label="181225-SD24_96SA-F-58H5"
                     /note="sequence: TATGTGTGGGAGGGCCAGGTAAGCGATAAGTATTCGATC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          1554..1578
                     /label="181225-SD24_96SA-F-58H5"
                     /note="sequence: TATGTGTGGGAGGGCCAGGTAAGCGATAAGTATTCGATC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1555..1562
                     /label="Splice_Donor"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          1555..1578
                     /label="190224-SD_MCS_SA-F-60B9"
                     /note="sequence: TATAGGGCGAATTGGGGTCTCACAGGTAAGCGATAAGTATTCGATC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     motif           1617..1637
                     /label="MS2_sirius1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(1634..1655)
                     /label="190926-MS2-sirius-seq-R-63I2(63I8)"
                     /note="sequence: ATGGTCCATCGTGGCATCGGAA"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     motif           1645..1665
                     /label="MS2_sirius2"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     motif           1673..1693
                     /label="MS2_sirius3"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     motif           1701..1721
                     /label="MS2_sirius4"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     motif           1729..1749
                     /label="MS2_sirius5"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     motif           1757..1777
                     /label="MS2_sirius6"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     motif           1785..1805
                     /label="MS2_sirius7"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     motif           1813..1831
                     /label="MS2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     motif           1845..1865
                     /label="MS2_sirius1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     motif           1873..1893
                     /label="MS2_sirius2"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     motif           1901..1921
                     /label="MS2_sirius3"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     motif           1929..1949
                     /label="MS2_sirius4"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     motif           1957..1977
                     /label="MS2_sirius5"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     motif           1985..2005
                     /label="MS2_sirius6"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     motif           2013..2033
                     /label="MS2_sirius7"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     regulatory      complement(2033..2042)
                     /label="Kozak sequence"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     motif           2041..2059
                     /label="MS2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     motif           2073..2093
                     /label="MS2_sirius1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     motif           2101..2121
                     /label="MS2_sirius2"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     motif           2129..2149
                     /label="MS2_sirius3"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     motif           2157..2177
                     /label="MS2_sirius4"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     motif           2185..2205
                     /label="MS2_sirius5"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     motif           2213..2233
                     /label="MS2_sirius6"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     motif           2241..2261
                     /label="MS2_sirius7"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     motif           2269..2287
                     /label="MS2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     binding_motif   complement(2345..2363)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(2374..2392)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(2403..2421)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(2448..2466)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(2477..2495)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(2506..2524)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(2551..2569)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(2580..2598)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(2609..2627)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(2654..2672)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(2683..2701)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(2712..2730)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(2757..2775)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(2786..2804)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(2815..2833)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(2860..2878)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(2889..2907)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(2918..2936)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(2963..2981)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(2992..3010)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3021..3039)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3066..3084)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3095..3113)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3124..3142)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3169..3187)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3198..3216)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3227..3245)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3272..3290)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3301..3319)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3330..3348)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3375..3393)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3404..3422)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3433..3451)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3478..3496)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3507..3525)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3536..3554)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3581..3599)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3610..3628)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3639..3657)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3684..3702)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3713..3731)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3742..3760)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3787..3805)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3816..3834)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3845..3863)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3890..3908)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3919..3937)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3948..3966)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(3993..4011)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4022..4040)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4051..4069)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          4070..4093
                     /label="191019-7xopto-TetO-R-65A9"
                     /note="sequence: CACCTCCTCTCCTAGTTACCACAA"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     binding_motif   complement(4096..4114)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4125..4143)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4154..4172)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4199..4217)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4228..4246)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4257..4275)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(4276..4301)
                     /label="191019-7xopto-TetO-F-65A8"
                     /note="sequence: GGCCCCGAATTCGCCTATTTGATTAACTAGGAGGTGGAAGG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     binding_motif   complement(4302..4320)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4331..4349)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4360..4378)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4405..4423)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4434..4452)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4463..4481)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4508..4526)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4537..4555)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4566..4584)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4611..4629)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4640..4658)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4669..4687)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4714..4732)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4743..4761)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4772..4790)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4817..4835)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4846..4864)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4875..4893)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4920..4938)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4949..4967)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(4978..4996)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(5023..5041)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(5052..5070)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(5081..5099)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(5126..5144)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(5155..5173)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(5184..5202)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(5229..5247)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(5258..5276)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(5287..5305)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(5332..5350)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(5361..5379)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(5390..5408)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(5435..5453)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(5464..5482)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(5493..5511)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(5538..5556)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(5567..5585)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     binding_motif   complement(5596..5614)
                     /label="TetO_binding_seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(5629..5648)
                     /label="190731-oligo1-sense-62H4"
                     /note="sequence: CGGGCCCCCCCTCGATAGGGATAACAGGGTAATCC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          5631..5664
                     /label="190804-NBSI-F-62I4"
                     /note="sequence: GCAGCCTAGTTAGGGGTCGACAACTAACCTAGCCATATTACCCTGTTATCCCTACTCGACATCGATTAGC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(5641..5664)
                     /label="190804-NBSI-R-62I5"
                     /note="sequence: GCTAATCGATGTCGAGTAGGGATA"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(5669..5708)
                     /label="190224-SD_MCS_SA-R-60C1"
                     /note="sequence: TCCCTGTGGGAGGAAGAGAAGAGGTCAGAAGCTTTATCGT"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    5675..5706
                     /label="Splice_Acceptor"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5681..5708)
                     /label="181225-SD24_96SA-R-58H6"
                     /note="sequence: CTGATCAGCGGGTTCCTAAAAAGCTGGTCCCTGTGGGAGGAAGAGAAGAGGTCAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(5681..5708)
                     /label="181225-SD24_96SA-R-58H6"
                     /note="sequence: CTGATCAGCGGGTTCCTAAAAAGCTGGTCCCTGTGGGAGGAAGAGAAGAGGTCAG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          5709..5730
                     /label="191019-IntN-F-64G9"
                     /note="sequence: ACACCACCAGCGGCCGCATCGATCACCGGTCGCCACCATGGTGGGGCCCGTACTTGTTTAGGTTCGCGAGGCTCACAGTGTCTGAGCTACGATACAGAGA"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          5709..5732
                     /label="190911-IntN-F-63E5"
                     /note="sequence: TGCCGGAGCAACCGGTGCCACCATGTGTCTGAGCTACGATACAGAGATC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          5709..5732
                     /label="200205-NNeoRC-F-68F9"
                     /note="sequence: TGTCTGAGCTACGATACAGAGATC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             5709..6011
                     /label="Cfa_intein_N"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          5802..5825
                     /label="190224-IntN-seqF-60C3"
                     /note="sequence: ACAGTGGACAAGAATGGCTTTGTC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          5878..5901
                     /label="190722-IntC-F-62E6 (62F4)"
                     /note="sequence: AGTATTGTCTCGAAGATGGGAGTA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(5952..5975)
                     /label="190722-IntC-R-62E7 (62F5)"
                     /note="sequence: CTCAAATATTTCGTCAATAGGCAG"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(5994..6017)
                     /label="191019-IntN-R-64H1"
                     /note="sequence: ATCGATGCCGCTTTATCCGGCGGGCAGCCCGTCAACCTG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          6037..6060
                     /label="190911-3xFLAG-F-63E8"
                     /note="sequence: AGCTTGCCACAACCCCGGGATAATTCCTGCGGGAAAGAGAAAACACATGTTT"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          6048..6071
                     /label="200301-FLAG-F-70B6"
                     /note="sequence: AAAAAAGAAAAGAAAAGTTGAAAAAACACATGTTTCCCGGCCTAGG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          6048..6071
                     /label="191201-3xFLAG-F-66F3"
                     /note="sequence: TGACCTCTTCTCTTCCTCCCACAGGGATGTCTGAGCAAAACACATGTTTCCCGGCCTAGG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     epitope         6072..6095
                     /label="FLAG-tag"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     epitope         6111..6134
                     /label="FLAG-tag"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     epitope         6150..6173
                     /label="FLAG-tag"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(6174..6197)
                     /label="191201-3xFLAG-R-66F4"
                     /note="sequence: GGGTTCCTAAAAAGCTGGTCTTACATGTTGCTATTAAGCCTAGGCCG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          6174..6199
                     /label="190817-N-F-63A5"
                     /note="sequence: CTAGGGCAGCCAATATGGGATCGGCCATTGAACGGCCTAGGCTTAATAGCAACATGGA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(6174..6199)
                     /label="200301-FLAG-R-70B7"
                     /note="sequence: CGCCCTTAGACACCATGGTGAATTCCATGTTGCTATTAAGCCTAGGCCG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             6174..6953
                     /label="NeoR (APH(3')-I family aminoglycoside O-phosphotransferase)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(6206..6233)
                     /label="190911-3xFLAG-R-63E9"
                     /note="sequence: TGCAGGAATTATCCCGTCGCGAGCCCACTTATATCCATAAAGG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(6210..6233)
                     /label="190728-MCS-R-62F8"
                     /note="sequence: GTCGCGAGCCCACTTATATCCATA"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          6215..6238
                     /label="190909-NeoR-F-63C1"
                     /note="sequence: ATATAAGTGGGCTCGCGACAATGT"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(6220..6244)
                     /label="190911-IntN-R-63E6"
                     /note="sequence: TGTCCTACATTGTCGCGAGCCCACT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(6354..6377)
                     /label="190722-NeoR-seqR-62E8 (62F6)"
                     /note="sequence: CATAAATTCTGTCAACCAGTTGAG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(6797..6820)
                     /label="190224-NeoR-seqR-60C4"
                     /note="sequence: TGGTATCTGTCGGCTATCCCAACG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(6929..6953)
                     /label="190911-3xFLAG-R-63F1"
                     /note="sequence: GAATTGATCCCCTCAGAAGAACTCGTCAAGCATCAGATGG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(6930..6955)
                     /label="190817-N-R-63A6"
                     /note="sequence: TGCTCCTGCTGTACCGAAGAACTCGTCAAGCATCAGATG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          6935..6959
                     /label="190926-NBSI-F-63F5"
                     /note="sequence: GACGAATTGTATAAGATGCTTGACGAGTTCTTCGGAACT"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          6939..6962
                     /label="190728-MCS-F-62F9"
                     /note="sequence: CTTGACGAGTTCTTCGGAACTGCT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(6939..6986)
                     /label="190803-NeoR-R-62H8"
                     /note="sequence: CTTTACAGCCCCAGCTCCAGCTCCAGCAGTTCCGAAGAACTCGTCAAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          6972..7070
                     /label="190803-NeoR-F-C-62H9"
                     /note="sequence: GCTGGGGCTGTAAAGGGAGGAGGTGGATCTGGTGGAGGAGGTAGTGGAGGAGGTGGAAGTGGTGGGGGAGTAAAGATCATCAGTCGAAAATCCCTGGGC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          7041..7064
                     /label="191019-IntC-F-64H6"
                     /note="sequence: TTTTTTCAGGTTGGACCGGTGCCACCATGGTGGTAAAGATCATCAGTCGAAAATCC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             7041..7151
                     /label="Cfa_intein_C_plusCF"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(7113..7139)
                     /label="200205-mutNNeoRC-R-68G2"
                     /note="sequence: CGTTGTCCCGAAGCAAGCGGCTGCAACCAGACCATTCTTCAGGAGAAA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(7124..7151)
                     /label="190909-NeoR-R-63C2"
                     /note="sequence: GTCTCAGAAGCAATTGCTTGCAACCAGACCATTC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(7128..7151)
                     /label="191019-IntC-R-64H7"
                     /note="sequence: GAAGCAATTGCTTGCAACCAGACC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(7128..7151)
                     /label="190718-IntC2-R-62F3"
                     /note="sequence: GGGGTCCGGCCTCGAGAAGCAATTGCTTGCAACCAGACC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          7132..7151
                     /label="190401-HA-left-61A3"
                     /note="sequence: TGGTTGCAAGCAATTGCTTC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          7137..7176
                     /label="200128-Nanog-3HAF-68B2"
                     /note="sequence: GCAAGCAATTGCTTCGAGAACTATTCTTGCTTACAAGGGT"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(7191..7214)
                     /label="190926-Nanog-3-150R-63G6"
                     /note="sequence: GGGAACAAAAGCTGGTTTGACATGCCTGCAAGTGCAGGTTCCTTACCAGCCTCTGTGCAGAG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(7323..7348)
                     /label="200227-Nanog-L-R-69H1"
                     /note="sequence: AATGCCTGGTGGTCATTCCGTCAGAGGGGTTTCCAGAAGAGTGATA"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(7343..7362)
                     /label="200227-sgNanog-sense-69I3"
                     /note="sequence: CACCGATCACCGGTCAAACTCAGAG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          7349..7372
                     /label="200227-Nanog-R-F-69H2"
                     /note="sequence: TGCATGGTTAGCTAGGGTACGTTTGACCGGTGATACGTTGGCCT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(7543..7564)
                     /label="190926-Nanog-3-500R-63G5"
                     /note="sequence: GGGAACAAAAGCTGGTTTGACATGCCTGCAAGTGCAGGAACTAACTGTATAACCTCACCA"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(7686..7715)
                     /label="190315int-Cas13-G1-S-9-60F5"
                     /note="sequence: AAACAAATCAAAAATTGAGGGAGGCAGAGGCAGAGTTGGGGTCTTCGAGAAGACCTTGAATGACTTAAACTGTGTACACTTGTTTG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          7737..7766
                     /label="190315-int-Cas13-G2-A-9-60F8"
                     /note="sequence: GTTGAGAGCATGTGTGTAGAGCATGTGTGTTTGTAGGTCTTCTCGAAGACCCCAACGCCAAGGTCTGAAGCTCATTGTCTGTACAG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(7959..8025)
                     /label="200128-Nanog-3HAR-68B3"
                     /note="sequence: GGGAACAAAAGCTGGTTTGACATGCCTGCAAGTGCAGGCAAGATGACAGACATTAACTTAAAAGCTC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(7961..7984)
                     /label="190926-Nanog-3-900R-63G4"
                     /note="sequence: GGGAACAAAAGCTGGTTTGACATGCCTGCAAGTGCAGGGATGACAGACATTAACTTAAAAGC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     motif           complement(7988..8010)
                     /label="target_seq_sgRNA_target"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     None            complement(7988..8010)
                     /label="Target_sequence"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          8011..8038
                     /label="180402-pBSK-BS-F-51D2"
                     /note="sequence: GATTTTTGAAATTTTGGAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          8015..8044
                     /label="180528-pBSK-F-53B3"
                     /note="sequence: CTTTTGTTCCCTTTAGTGAGGGTTAATTGC"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          8016..8039
                     /label="170610-pBSK-F-46A5"
                     /note="sequence: ATCGATGGCTCGTCTCACTGTTTTTGTTCCCTTTAGTGAGGGTTA"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          8016..8039
                     /label="170610-pBSK-F-45H1"
                     /note="sequence: ATCGATGGCTCGTCTCACTGTTTTTGTTCCCTTTAGTGAGGGTTA"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          8016..8039
                     /label="170611-pBSK-F-45I7"
                     /note="sequence: GAATGGTCTTTCTGATTTTGTTCCCTTTAGTGAGGGTTA"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          8016..8039
                     /label="170610-pBSK-F-45H9"
                     /note="sequence: AGAGTTGGTTGAAGGTTTTGTTCCCTTTAGTGAGGGTTA"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          8016..8045
                     /label="190315-pBSK-F-60D7"
                     /note="sequence: AAACATGTCTTCCTATTTTGTTCCCTTTAGTGAGGGTTAATTGCG"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          8016..8045
                     /label="180801-pBSK-F-53F8"
                     /note="sequence: AACACCGGGTCTTCGTTTTGTTCCCTTTAGTGAGGGTTAATTGCG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer_bind     complement(8022..8041)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(8024..8042)
                     /label="T3 promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     complement(8026..8041)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(8026..8042)
                     /label="T3 promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     complement(8059..8079)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(8063..8084)
                     /label="170612-M13-R-46C1"
                     /note="sequence: TCACACAGGAAACAGCTATGAC"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(8063..8084)
                     /label="200104-M13-R-67C2 (67C5)"
                     /note="sequence: TCACACAGGAAACAGCTATGAC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(8063..8084)
                     /label="190810-M13-R-62I6"
                     /note="sequence: TCACACAGGAAACAGCTATGAC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_binding    complement(8085..8107)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(8111..8141)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     Promoter        complement(8112..8141)
                     /label="lac"
                     /ApEinfo_revcolor=#4682b4
                     /ApEinfo_fwdcolor=#4682b4
     primer          complement(8133..8156)
                     /label="170828-tdmRuby2-R-47G9"
                     /note="sequence: TTAGGCACCCCAGGCTTTACACTT"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          8142..8165
                     /label="170828-tdiRFP-F-47G7"
                     /note="sequence: GCCTGGGGTGCCTAATGAGTGAGC"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(8257..8280)
                     /label="181225-delta-Sap-R-58E2"
                     /note="sequence: GACCGAGCGCGCTCTTCAAGCGCCCAATACGCAAACCGCCTCTCC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(8257..8280)
                     /label="181225-delta-sapI-pB-R-58H1"
                     /note="sequence: GCCCAATACGCAAACCGCCTCTCC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          8281..8304
                     /label="181225-delta-sapI-pB-F-58G9"
                     /note="sequence: CGCTTCCTCGCTCACTGACTCGCT"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(8412..8435)
                     /label="200309-sgRNA_PCR-F-70F1"
                     /note="sequence: TACGGTTCCTGGCCTTTTGCTGGC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(8431..8454)
                     /label="190326-gRNA2-F-60H9"
                     /note="sequence: GCTATGCTGTTTTTTGAGGGGCCAGCAACGCGGCCTTTTTACGG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(8440..9068)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#4682b4
                     /ApEinfo_fwdcolor=#4682b4
     rep_origin      complement(8440..9122)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(8496..8519)
                     /label="190716-ColE1-seqF-62E4"
                     /note="sequence: GCCACCTCTGACTTGAGCGTCGAT"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          9116..9150
                     /label="181107-LWLP-F-56G8"
                     /note="sequence: GTCATGAGATTATCAAAAAGGATCTTCACCTAGAT"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          9220..9243
                     /label="171113-Amp-probe-F-48D7"
                     /note="sequence: CCAATGCTTAATCAGTGAGGCACC"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     CDS             complement(9220..10074)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(9346..9371)
                     /label="190926-NBSIR-63F6"
                     /note="sequence: GTGAGCGTGGCAGCCGCGGTATCATT"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          9644..9667
                     /label="191019-down-R-64H3"
                     /note="sequence: TCCTTCGGTCCTCCGATCGTTGTC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(9942..9965)
                     /label="171113-Amp-probe-R-48D8"
                     /note="sequence: TGGGTGCACGAGTGGGTTACATCG"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     Promoter        complement(10119..10147)
                     /label="Amp prom"
                     /ApEinfo_revcolor=#4682b4
                     /ApEinfo_fwdcolor=#4682b4
ORIGIN
        1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
       61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
      121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC
      181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
      241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG
      301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA
      361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC
      421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCAT TCAGGCTGCG
      481 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG
      541 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG
      601 TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT TGGTTTGACA
      661 TGCCTGCAAG TGCAGGGCAT GGACCAACTT ACTAAGGTAG CCCGAGTCTT AAGCAGGACA
      721 CAGGCTCTTT CTTCAGACTT GCGTTAAAAA GCCGCACTTT TGGAGGGAAG ATTTCCCCAG
      781 GTTTCCCAAT GTGAAGAGCA AGCAAGAAAC GCTGAGTGCT GAAAGGAAAG CCGTGTATAA
      841 ACAGAGACTT TGGCAGCAAG GTCTGACTCT TTCATGTCTG TAGAAAGAAT GGAAGAGGAA
      901 ACTCAGATCC CCCACTTGAC CTGAAACTTC CCACTAGAGA TCGCCAGGGT CTGGAGGTGC
      961 AGCCGTGGTT AAAAGATGAA TAAAGTGAAA TGAGGTAAAG CCTCTTTTTG GGGGGGGGGG
     1021 ATGTCTTTAG ATCAGAGGAT GCCCCCTAAG CTTTCCCTCC CTCCCAGTCT GGGTCACCTT
     1081 ACAGCTTCTT TTGCATTACA ATGTCCATGG TGGACCCTGC AGGTGGGATT AACTGTGAAT
     1141 TCACAGGGCT GGTGGGGCGT GGGTGCCGCC TGGGTGCCTG GGAGAATAGG GGGTGGGTAG
     1201 GGTAGGAGGC TTGAGGGGGG AGGAGCAGGA CCTACCCTTT AAATCTATCG CCTTGAGCCG
     1261 TTGGCCTTCA GATAGGCTGA TTTGGTTGGT GTCTTGCTCT TTCTGTGGGA AGGCTGCGGC
     1321 TCACTTCCTT CTGACTTCTT GATAATTTTG CATTAGACAT TTAACTCTTC TTTCTATGAT
     1381 CTTTCCTTCT AGACACTGAG TTTTTTGGTT GTTGCCTAAA ACCTTTTCAG AAATCCCTTC
     1441 CCTCGCCATC ACACTGACAT GAGTGTGGGT CTTCCTGGTC CCCACAGTTT GCCTAGTTCT
     1501 GAGGAAGCAT CGAATTCTGG GAACGCCTCA TCAATGCCTG CAGTTTTTCA TCCCCAGGTA
     1561 AGCGATAAGT ATTCGATCAT GCATTGGTTA GCGGCCGCGA ATTCGCCCTT GGATCCGGAA
     1621 TGACCACCAG GCATTCCGAT GCCACGATGG ACCATCAGGC CATCGAGCTG CAGAAGTGAC
     1681 GACCACGCAC TTCGGAGTGA CAGAGGAGGA TCACCCCTCT GGCCACCAGA GTAGAGCATC
     1741 AGCCTACTCA CAGAGCCTAC GGAGGACCAC CCCGTAGGAG TAGAGCGAGG AGCACCAGCC
     1801 CTCGCGTGTG ACGCACGAGC ATCAGCCGTG CGATGGTAAG ATCCGGAATG ACCACCAGGC
     1861 ATTCCTCCAA GGCGATGGAC CATCAGGCCA TCGAGGACTC GAAGTGACGA CCACGCACTT
     1921 CGTGAAGGCA GAGGAGGATC ACCCCTCTGC CCACAGGAGT AGAGCATCAG CCTACTCCAA
     1981 GCAGCTACGG AGGACCACCC CGTAGAAGTA GGGCGAGGAG CACCAGCCCT CGCCATGGTG
     2041 GCACGAGCAT CAGCCGTGCA GGGTATAGAT CCGGAATGAC CACCAGGCAT TCCTGGCCAA
     2101 CGATGGACCA TCAGGCCATC GGCATAGCGA AGTGACGACC ACGCACTTCA GGGTAGCAGA
     2161 GGAGGATCAC CCCTCTGGCC ACACGAGTAG AGCATCAGCC TACTCCACAG GACTACGGAG
     2221 GACCACCCCG TAGAAAGTGG GCGAGGAGCA CCAGCCCTCG CCGTGATGGC ACGAGCATCA
     2281 GCCGTGCGTT AGGAAGATCT CGATTCGATG CAGCCTAGTT AGGGGTCGAC TAGTCTACTC
     2341 ACTATCTCTA TCACTGATAG GGAAACCTTC CTCTCTCTAT CACTGATAGG GACCTTTTCC
     2401 CTTCTCTATC ACTGATAGGG ACCCATATTC TCCTAGTTTA ACTTCTATCT CTATCACTGA
     2461 TAGGGAAACT CACTTCTCTC TATCACTGAT AGGGACCTTA TCCCCTCTCT ATCACTGATA
     2521 GGGATATTAT ATTTCCTAGT ATTCACCTCC TCTCTATCAC TGATAGGGAT TCACCATTTT
     2581 CTCTATCACT GATAGGGATA TTTCCTTCTC TCTATCACTG ATAGGGACAT ATTTTTCCCT
     2641 AGTCAATCCT CACTCTCTAT CACTGATAGG GACTAACAAA ATTCTCTATC ACTGATAGGG
     2701 AAAACCAAAC ATCTCTATCA CTGATAGGGA TCATCATTTT CCTAGTATAA CCATACTCTC
     2761 TATCACTGAT AGGGATCACC CCCACTCTCT ATCACTGATA GGGATTACAC AACTTCTCTA
     2821 TCACTGATAG GGACTCTCTT TATCCTAGTC ACATTAACAT CTCTATCACT GATAGGGACA
     2881 CCTTTTCATC TCTATCACTG ATAGGGACAT CCATTATTCT CTATCACTGA TAGGGACATT
     2941 AACTCTCCTA GTAAAATCCC CATCTCTATC ACTGATAGGG AAACCTCATC ATCTCTATCA
     3001 CTGATAGGGA ATCTATTCCA TCTCTATCAC TGATAGGGAT ATCTTTTATC CTAGTCCTAA
     3061 CATCATCTCT ATCACTGATA GGGAACCCAA ACTTTCTCTA TCACTGATAG GGACCAATTT
     3121 ACCTCTCTAT CACTGATAGG GAATCTTCTT ATCCTAGTAC AAAACTAATC TCTATCACTG
     3181 ATAGGGAATC CCTCCCTTCT CTATCACTGA TAGGGATACC TTTCCTTCTC TATCACTGAT
     3241 AGGGAATTAC TTTTACCTAG TAATACCTCA ATCTCTATCA CTGATAGGGA AAATCACTAA
     3301 TCTCTATCAC TGATAGGGAT TTTTATTATT CTCTATCACT GATAGGGAAC TCCTTTAACC
     3361 TAGTCTATTC CATTTCTCTA TCACTGATAG GGATTCACTA CCCTCTCTAT CACTGATAGG
     3421 GATTATTTCT TCTCTCTATC ACTGATAGGG AATTTTTCCT TCCTAGTACC ATAACCCTCT
     3481 CTATCACTGA TAGGGAACAC CAATATTCTC TATCACTGAT AGGGACATTA TTTATTCTCT
     3541 ATCACTGATA GGGAATTATT ACTCCCTAGT TCTTACATTA TCTCTATCAC TGATAGGGAA
     3601 CCCCTCTCCT CTCTATCACT GATAGGGATA TCAATCACTC TCTATCACTG ATAGGGACCT
     3661 ATTATACCCT AGTCCAATAT ATATCTCTAT CACTGATAGG GACCACACTT CTTCTCTATC
     3721 ACTGATAGGG ATCACCTTAT ATCTCTATCA CTGATAGGGA CACTCTCTAT CCTAGTCCAA
     3781 TCCTTTTCTC TATCACTGAT AGGGATAAAC ACATCTCTCT ATCACTGATA GGGATACATT
     3841 TTACTCTCTA TCACTGATAG GGATTCTCCC TTTCCTAGTC TTAACTTCTT CTCTATCACT
     3901 GATAGGGATT TTTCCAATTC TCTATCACTG ATAGGGAATC ACTTCATTCT CTATCACTGA
     3961 TAGGGATTAT TTCTCCCCTA GTTCACTCCT ACTCTCTATC ACTGATAGGG ACTCCCAATA
     4021 TTCTCTATCA CTGATAGGGA TATTTTTCTC TCTCTATCAC TGATAGGGAC ACCTCCTCTC
     4081 CTAGTTACCA CAACTTCTCT ATCACTGATA GGGAATACTC TCAATCTCTA TCACTGATAG
     4141 GGATTTCTAT TTCTCTCTAT CACTGATAGG GAATACCCTC TACCTAGTAA CAAATCTTTC
     4201 TCTATCACTG ATAGGGAATA ATATATCTCT CTATCACTGA TAGGGATCTT ATACCCTCTC
     4261 TATCACTGAT AGGGACCTTC CACCTCCTAG TTAATCAAAT ATCTCTATCA CTGATAGGGA
     4321 TAATCTACTT TCTCTATCAC TGATAGGGAA ACTCATCCAT CTCTATCACT GATAGGGATT
     4381 TTTAACTTCC TAGTATCCAT TCTATCTCTA TCACTGATAG GGATAATACA AACTCTCTAT
     4441 CACTGATAGG GATACTCTCC TTTCTCTATC ACTGATAGGG ACCTTATTTT CCCTAGTATA
     4501 ATCCCTATCT CTATCACTGA TAGGGAACAT TAACTATCTC TATCACTGAT AGGGATTTTA
     4561 TTCCTTCTCT ATCACTGATA GGGAACTTAC CTATCCTAGT ATACCCATAA TCTCTATCAC
     4621 TGATAGGGAA ACCAAAATTT CTCTATCACT GATAGGGAAT CCAATTACTC TCTATCACTG
     4681 ATAGGGATTT CTATCTCCCT AGTTTAAAAC CAATCTCTAT CACTGATAGG GAACCCCTAA
     4741 ACTCTCTATC ACTGATAGGG ATTTCTCTTA TTCTCTATCA CTGATAGGGA CCTTCTCTCA
     4801 CCTAGTCTAC TCACTATCTC TATCACTGAT AGGGAAACCT TCCTCTCTCT ATCACTGATA
     4861 GGGACCTTTT CCCTTCTCTA TCACTGATAG GGACCCATAT TCTCCTAGTA CACTATAAAT
     4921 CTCTATCACT GATAGGGAAA ACATACCTTC TCTATCACTG ATAGGGACTA TATTTTTTCT
     4981 CTATCACTGA TAGGGATCTA ATACTTCCTA GTACTTTAAA TATCTCTATC ACTGATAGGG
     5041 AATCTACTAA ATCTCTATCA CTGATAGGGA CCCATAAACT TCTCTATCAC TGATAGGGAT
     5101 ATTCTTTAAC CTAGTACAAT ACCATTCTCT ATCACTGATA GGGACCCCCC TAATTCTCTA
     5161 TCACTGATAG GGAATTTTTT TCCTCTCTAT CACTGATAGG GATTATCTCT TCCCTAGTTC
     5221 CTCTTAAATC TCTATCACTG ATAGGGATCT CACTACTTCT CTATCACTGA TAGGGAAACT
     5281 CATAACTCTC TATCACTGAT AGGGAAAATT ATCTTCCTAG TAAAACACCT ATCTCTATCA
     5341 CTGATAGGGA TATTATACCA TCTCTATCAC TGATAGGGAT TTATCATTTT CTCTATCACT
     5401 GATAGGGATT TTTACCATCC TAGTTAAAAA TATCTCTCTA TCACTGATAG GGATCACTAA
     5461 CTCTCTCTAT CACTGATAGG GAACTATTCT ATTCTCTATC ACTGATAGGG ACACTTTTTA
     5521 ACCTAGTACA TTACCCATCT CTATCACTGA TAGGGAACCT ATCTCTTCTC TATCACTGAT
     5581 AGGGATATTT TTTCATCTCT ATCACTGATA GGGAAAACTT TACACCTAGG ATTACCCTGT
     5641 TATCCCTACT CGACATCGAT TAGCTGATAC GATAAAGCTT CTGACCTCTT CTCTTCCTCC
     5701 CACAGGGATG TCTGAGCTAC GATACAGAGA TCCTCACAGT TGAGTACGGA TTTCTGCCCA
     5761 TTGGCAAAAT AGTCGAGGAA AGGATTGAAT GCACAGTTTA CACAGTGGAC AAGAATGGCT
     5821 TTGTCTATAC ACAGCCAATA GCCCAGTGGC ATAACCGAGG AGAGCAAGAG GTATTCGAGT
     5881 ATTGTCTCGA AGATGGGAGT ATCATCCGAG CCACCAAAGA CCACAAATTC ATGACAACAG
     5941 ACGGACAAAT GCTGCCTATT GACGAAATAT TTGAGAGAGG TTTGGATCTG AAGCAGGTTG
     6001 ACGGGCTGCC CGCCGGAGCC GGTGCCGGAG CTGCAAGCGG GAAAGAGAAA ACACATGTTT
     6061 CCCGGCCTAG GGATTACAAG GATGACGACG ATAAGGGCAC TGCAAGCAGT GACTATAAGG
     6121 ATGATGATGA CAAAGGGTCT GGTGGGGGCG ACTATAAGGA TGACGATGAC AAGCGGCCTA
     6181 GGCTTAATAG CAACATGGAC GCAGACCTTT ATGGATATAA GTGGGCTCGC GACAATGTAG
     6241 GACAGAGCGG CGCTACTATT TATCGGCTCT ATGGAAAACC CGATGCTCCC GAGCTGTTCC
     6301 TGAAGCACGG CAAGGGATCC GTAGCAAACG ACGTTACAGA CGAAATGGTA CGACTCAACT
     6361 GGTTGACAGA ATTTATGCCC CTGCCCACTA TCAAGCACTT CATAAGAACT CCTGATGACG
     6421 CTTGGCTGCT GACAACCGCA ATCCCCGGTA AAACAGCATT CCAGGTCCTT GAAGAGTATC
     6481 CAGACTCTGG TGAGAATATT GTGGACGCAC TGGCAGTATT CCTCAGGCGG CTCCACAGTA
     6541 TTCCTGTTTG CAATTGTCCC TTTAACTCTG ACAGGGTATT CAGGCTCGCC CAGGCCCAAT
     6601 CACGAATGAA TAATGGCTTG GTTGATGCAA GCGATTTCGA TGATGAACGC AATGGCTGGC
     6661 CTGTTGAACA AGTTTGGAAA GAAATGCACA AGCTCCTGCC CTTTTCACCC GATTCTGTAG
     6721 TGACTCACGG GGACTTCAGC CTCGACAATT TGATCTTCGA TGAAGGAAAG CTTATCGGCT
     6781 GCATAGATGT TGGGCGCGTT GGGATAGCCG ACAGATACCA GGACCTGGCC ATATTGTGGA
     6841 ATTGTCTCGG CGAGTTTTCC CCTAGCTTGC AAAAACGGCT CTTTCAGAAG TATGGCATAG
     6901 ATAACCCCGA CATGAATAAA CTTCAATTCC ATCTGATGCT TGACGAGTTC TTCGGAACTG
     6961 CTGGAGCTGG AGCTGGGGCT GTAAAGGGAG GAGGTGGATC TGGTGGAGGA GGTAGTGGAG
     7021 GAGGTGGAAG TGGTGGGGGA GTAAAGATCA TCAGTCGAAA ATCCCTGGGC ACCCAAAATG
     7081 TATACGATAT TGGGGTCGAG AAAGATCACA ATTTTCTCCT GAAGAATGGT CTGGTTGCAA
     7141 GCAATTGCTT CGAGAACTAT TCTTGCTTAC AAGGGTCTGC TACTGAGATG CTCTGCACAG
     7201 AGGCTGGTAA GGAATTCAGT CCCCGAAGAA CCCAGTAAAT TAGGGTAAGA GTAGAAAAAA
     7261 CGGGCTGAAG GGTTATTGCT TCTTTATGAA CCGTAGTAGT CATTAACATA AGCGGGTGTC
     7321 CTTATCACTC TTCTGGAAAC CCCTCTGAGT TTGACCGGTG ATACGTTGGC CTTCTAGTCT
     7381 GAAATAGAGA TCCGGGACAC AGGACGGAGC AATATAAGGC TCTTGTGTGT ATTTGAGAAC
     7441 CTAAATATTT GGGCTCTTTG GAATATGTTC GGGGGCAGTG AGTGGTGTCT TCAGTAGCAG
     7501 GGAGACCTAA CATCCTAGCT TGGAATGAGC ACCCCATTTT TTTGGTGAGG TTATACAGTT
     7561 AGTTTGCAAT TTGCCCAAAT GGCCGTTTTC TCCAGCAGAA GCAGTTAGCA TTTCTCTCAA
     7621 CCTTTGGGAA TATGGATGTC ACTTAGCAGT TCTGGGTTTG TGAGCTCAGT GCTCCTTCCA
     7681 AACCCCAAAC AAGTGTACAC AGTTTAAGTC ATTCATTCTT ATAGCTAGCA TCTGGAGCCA
     7741 AGGTCTGAAG CTCATTGTCT GTACAGAGTC AGCCCAGTAC TCAGGCTTGT AAATGGTAGA
     7801 ACCAAGAGGC TGCTAGGCTG TGTGTGCCAC GGTGCTCAGG TGCGTGTTGT GATGCTCGGG
     7861 TGTGTGCAGC GATGCTCGGG TGTGTGCAGT GATGCTCGGG TGTGTGCAGC GGTGCTTGGG
     7921 TGTGTGGAGC GCTAGGAAGT GTGTGTCCCC TTTTTTCTGA GCTTTTAAGT TAATGTCTGT
     7981 CATCTTGCCT GCACTTGCAG GCATGTCAAA CCAGCTTTTG TTCCCTTTAG TGAGGGTTAA
     8041 TTGCGCGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA
     8101 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG
     8161 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT
     8221 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC
     8281 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC
     8341 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT
     8401 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
     8461 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     8521 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     8581 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     8641 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     8701 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     8761 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     8821 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     8881 CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     8941 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     9001 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
     9061 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT
     9121 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC
     9181 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC
     9241 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA
     9301 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGGCT
     9361 GCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG
     9421 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
     9481 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT
     9541 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG
     9601 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
     9661 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA
     9721 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
     9781 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA
     9841 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG
     9901 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
     9961 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG
    10021 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT
    10081 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
    10141 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT
    10201 GCCAC
//