LOCUS       pAS_4xMmaPylT(UUA)_EF1_ 8549 bp ds-DNA     circular     06-AUG-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     772..791
                     /label="BII(R)"
     misc_feature    complement(834..846)
                     /label="3'TR"
     misc_feature    847..877
                     /label="31bp spacer"
     misc_feature    complement(847..877)
                     /label="3'TR"
     misc_feature    complement(878..879)
                     /label="3'TR"
     misc_feature    complement(881..896)
                     /label="3'TR"
     enhancer        1072..1303
                     /label="core insul"
     misc_feature    complement(1341..1409)
                     /label="PylT TAA"
     misc_feature    complement(1410..1652)
                     /label="h7SKpromoter"
     misc_feature    complement(1670..1738)
                     /label="PylT TAA"
     misc_feature    complement(1739..1981)
                     /label="h7SKpromoter"
     misc_feature    complement(1999..2067)
                     /label="PylT TAA"
     misc_feature    complement(2068..2310)
                     /label="h7SKpromoter"
     misc_feature    complement(2328..2396)
                     /label="PylT TAA"
     misc_feature    complement(2397..2639)
                     /label="h7SKpromoter"
     promoter        2655..3200
                     /label="EF1"
     misc_feature    3219..3944
                     /label="sfGFP 150TAA"
     CDS             3219..3944
                     /label="Translation 3219-3944"
     misc_feature    3947..4535
                     /label="IRES"
     misc_feature    4536..4544
                     /label="Kozak consensus sequences"
     CDS             4541..4951
                     /label="Translation 4541-4951"
     misc_feature    4553..4948
                     /label="Blasticidin Resistance Gene (bsd)"
     misc_feature    5074..5077
                     /label="SV40 pA"
     misc_feature    5083..5209
                     /label="SV40 pA"
     enhancer        complement(5458..5688)
                     /label="core insul"
     misc_feature    5963..5997
                     /label="5'TR"
     primer_bind     complement(6516..6535)
                     /label="pLac(F)"
     CDS             complement(7652..8509)
                     /label="Amp"
ORIGIN
        1 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT
       61 CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT
      121 TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA
      181 TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA
      241 AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG
      301 GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA
      361 AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG
      421 CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA
      481 GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG
      541 GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT
      601 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA
      661 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA
      721 TTCACGTTTT TGAACCCGTG GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG
      781 TGATGGAGCA GATGAAGATG CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA
      841 AAGATAATCA TATTGTGACG TACGTTAAAG ATAATCATGT GTAAAATTGA CGCATGTGTT
      901 TTATCGGTCT GTATATCGAG GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA
      961 TTTACACTTA CATACTAATA ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT
     1021 TAAAAAAAAC AAAAACTCAA AATTTCTTCT ATAAAGTAAC AAAACTTTTA TGAGGGACAG
     1081 CCCCCCCCCA AAGCCCCCAG GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG
     1141 AGCCGCCCGG GGCTCCGCTC CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG
     1201 CGGGGACAGC CCGGGCACGG GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG
     1261 CTGCTCTTTG AGCCTGCAGA CACCTGGGGG GATACGGGGA AAAGGCCTCC ACgGCCaact
     1321 agTGAAAAAC CGCACTTGTC CGGAAACCCC GGGAATCTAA CCCGGCTGAA CGGATTTAAA
     1381 GTCCGTTCGA TCTACATGAT CAGGTTTCCG AGGTACCCAG GCGGCGCACA AGCTATATAA
     1441 ACCTGAAGGA AATCTCAACT TTACACTTAG GTCAAGTTAC TTATCGTACT AGAGCTTCAG
     1501 CAGGAAATTT AACTAAAATC TAATTTAACC AGCATAGCAA ATATCATTTA TTCCCAAAAT
     1561 GCTAAAGTTT GAGATAAACG GACTTGATTT CCGGCTGTTT TGACACTATC CAGAATGCCT
     1621 TGCAGATGGG TGGGGCATGC TAAATACTGC AGGAAAAACC GCACTTGTCC GGAAACCCCG
     1681 GGAATCTAAC CCGGCTGAAC GGATTTAAAG TCCGTTCGAT CTACATGATC AGGTTTCCGA
     1741 GGTACCCAGG CGGCGCACAA GCTATATAAA CCTGAAGGAA ATCTCAACTT TACACTTAGG
     1801 TCAAGTTACT TATCGTACTA GAGCTTCAGC AGGAAATTTA ACTAAAATCT AATTTAACCA
     1861 GCATAGCAAA TATCATTTAT TCCCAAAATG CTAAAGTTTG AGATAAACGG ACTTGATTTC
     1921 CGGCTGTTTT GACACTATCC AGAATGCCTT GCAGATGGGT GGGGCATGCT AAATACTGCA
     1981 GGAAAAACCG CACTTGTCCG GAAACCCCGG GAATCTAACC CGGCTGAACG GATTTAAAGT
     2041 CCGTTCGATC TACATGATCA GGTTTCCGAG GTACCCAGGC GGCGCACAAG CTATATAAAC
     2101 CTGAAGGAAA TCTCAACTTT ACACTTAGGT CAAGTTACTT ATCGTACTAG AGCTTCAGCA
     2161 GGAAATTTAA CTAAAATCTA ATTTAACCAG CATAGCAAAT ATCATTTATT CCCAAAATGC
     2221 TAAAGTTTGA GATAAACGGA CTTGATTTCC GGCTGTTTTG ACACTATCCA GAATGCCTTG
     2281 CAGATGGGTG GGGCATGCTA AATACTGCAG GAAAAACCGC ACTTGTCCGG AAACCCCGGG
     2341 AATCTAACCC GGCTGAACGG ATTTAAAGTC CGTTCGATCT ACATGATCAG GTTTCCGAGG
     2401 TACCCAGGCG GCGCACAAGC TATATAAACC TGAAGGAAAT CTCAACTTTA CACTTAGGTC
     2461 AAGTTACTTA TCGTACTAGA GCTTCAGCAG GAAATTTAAC TAAAATCTAA TTTAACCAGC
     2521 ATAGCAAATA TCATTTATTC CCAAAATGCT AAAGTTTGAG ATAAACGGAC TTGATTTCCG
     2581 GCTGTTTTGA CACTATCCAG AATGCCTTGC AGATGGGTGG GGCATGCTAA ATACTGCAGA
     2641 Actagtgctc tagaaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca
     2701 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga
     2761 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     2821 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     2881 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc
     2941 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
     3001 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc
     3061 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
     3121 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc
     3181 aagctgtgac cggcgcctac tctagagCTA GCGCCACCAT GGTGTCCAAG GGCGAGGAAC
     3241 TGTTCACCGG CGTGGTGCCC ATCCTGGTGG AACTGGATGG CGACGTGAAC GGCCACAAGT
     3301 TCTCTGTGCG GGGAGAGGGC GAAGGCGACG CCACAAATGG CAAGCTGACC CTGAAGTTCA
     3361 TCTGCACCAC CGGCAAGCTG CCCGTGCCTT GGCCTACCCT CGTGACCACA CTGACCTACG
     3421 GCGTGCAGTG CTTCAGCAGA TACCCCGACC ATATGAAGCG GCACGACTTC TTCAAGAGCG
     3481 CCATGCCCGA GGGCTACGTG CAGGAACGGA CCATCAGCTT CAAGGACGAC GGCACCTACA
     3541 AGACCAGAGC CGAAGTGAAG TTCGAGGGCG ACACCCTCGT GAACCGGATC GAGCTGAAGG
     3601 GCATCGATTT CAAAGAGGAC GGCAACATCC TGGGCCACAA GCTGGAGTAC AACTTCAACA
     3661 GCCACTAAGT GTACATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC AACTTCAAGA
     3721 TCCGGCACAA CGTGGAAGAT GGCAGCGTGC AGCTGGCCGA CCACTACCAG CAGAACACCC
     3781 CCATCGGAGA TGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGAGCGTGC
     3841 TGAGCAAGGA CCCCAACGAG AAGCGGGACC ACATGGTGCT GCTGGAATTC GTGACCGCCG
     3901 CTGGCATCAC CCACGGCATG GACGAGCTGT ACAAGGGCAG CTGAgcGGCC GCgcccctct
     3961 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     4021 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     4081 ggccctgtct tcttgacgag cattcctagg ggtctttccc Ctctcgccaa aggaatgcaa
     4141 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     4201 tctgTagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     4261 caaaagccac gtgtataaga tacacctgCa aaggcggcac aaccccagtg ccacgttgtg
     4321 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caAggggctg
     4381 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     4441 tttacatgtg tttagtCgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     4501 gttttccttt gaaaaaCACG ATGATAATAT GGCCACAACC ATGgcgtccg gaATGGCCAA
     4561 GCCTTTGTCT CAAGAAGAAT CCACCCTCAT TGAAAGAGCA ACGGCTACAA TCAACAGCAT
     4621 CCCCATCTCT GAAGACTACA GCGTCGCCAG CGCAGCTCTC TCTAGCGACG GCCGCATCTT
     4681 CACTGGTGTC AATGTATATC ATTTTACTGG GGGACCTTGT GCAGAACTCG TGGTGCTGGG
     4741 CACTGCTGCT GCTGCGGCAG CTGGCAACCT GACTTGTATC GTCGCGATCG GAAATGAGAA
     4801 CAGGGGCATC TTGAGCCCCT GCGGACGGTG CCGACAGGTG CTTCTCGATC TGCATCCTGG
     4861 GATCAAAGCC ATAGTGAAGG ACAGTGATGG ACAGCCGACG GCAGTTGGGA TTCGTGAATT
     4921 GCTGCCCTCT GGTTATGTGT GGGAGGGCTG ACCATGggtc gacaatcaac ctctggatta
     4981 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     5041 atacgctgct ttaatgcctt tgtatcatgc GTTAACTaaa ctTGTTTATT GCAGCTTATA
     5101 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC
     5161 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG AATTGACTCA
     5221 AATGATGTCA ATTAGTCTAT CAGAAGCTCA TCTGGTCTCC CTTCCGGGGG ACAAGACATC
     5281 CCTGTTTAAT ATTTAAACAG CAGTGTTCCC AAACTGGGTT CTTATATCCC TTGCTCTGGT
     5341 CAACCAGGTT GCAGGGTTTC CTGTCCTCAC AGGAACGAAG TCCCTAAAGA AACAGTGGCA
     5401 GCCAGGTTTA GCCCCGGAAT TGACTGGATT CCTTTTTTAG GGCCCATTGG TATGGCTTTT
     5461 TCCCCGTATC CCCCCAGGTG TCTGCAGGCT CAAAGAGCAG CGAGAAGCGT TCAGAGGAAA
     5521 GCGATCCCGT GCCACCTTCC CCGTGCCCGG GCTGTCCCCG CACGCTGCCG GCTCGGGGAT
     5581 GCGGGGGGAG CGCCGGACCG GAGCGGAGCC CCGGGCGGCT CGCTGCTGCC CCCTAGCGGG
     5641 GGAGGGACGT AATTACATCC CTGGGGGCTT TGGGGGGGGG CTGTCCCTGA TATCTATAAC
     5701 AAGAAAATAT ATATATAATA AGTTATCACG TAAGTAGAAC ATGAAATAAC AATATAATTA
     5761 TCGTATGAGT TAAATCTTAA AAGTCACGTA AAAGATAATC ATGCGTCATT TTGACTCACG
     5821 CGGTCGTTAT AGTTCAAAAT CAGTGACACT TACCGCATTG ACAAGCACGC CTCACGGGAG
     5881 CTCCAAGCGG CGACTGAGAT GTCCTAAATG CACAGCGACG GATTCGCGCT ATTTAGAAAG
     5941 AGAGAGCAAT ATTTCAAGAA TGCATGCGTC AATTTTACGC AGACTATCTT TCTAGGGTTA
     6001 ATCTAGCTGC ATCAGGATCA TATCGTCGGG TCTTTTTTCC GGCTCAGTCA TCGCCCAAGC
     6061 TGGCGCTATC TGGGCATCGG GGAGGAAGAA GCCCGTGCCT TTTCCCGCGA GGTTGAAGCG
     6121 GCATGGAAAG AGTTTGCCGA GGATGACTGC TGCTGCATTG ACGTTGAGCG AAAACGCACG
     6181 TTTACCATGA TGATTCGGGA AGGTGTGGCC ATGCACGCCT TTAACGGTGA ACTGTTCGTT
     6241 CAGGCCACCT GGGATACCAG TTCGTCGCGG CTTTTCCGGA CACAGTTCCG GATGGTCAGC
     6301 CCGAAGCGCA TCAGCAACCC GAACAATACC GGCGACAGCC GGAACTGCCG TGCCGGTGTG
     6361 CAGATTAATG ACAGCGGTGC GGCGCTGGGA TATTACGTCA GCGAGGACGG GTATCCTGGC
     6421 TGGATGCCGC AGAAATGGAC ATGGATACCC CGTGAGTTAC CCGGCGGGCG CGCTTGGCGT
     6481 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA
     6541 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT
     6601 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT
     6661 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT
     6721 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA
     6781 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA
     6841 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC
     6901 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA
     6961 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC
     7021 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT
     7081 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT
     7141 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG
     7201 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA
     7261 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT
     7321 ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA
     7381 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT
     7441 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA
     7501 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT
     7561 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA
     7621 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT
     7681 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA
     7741 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT
     7801 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG
     7861 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA
     7921 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT
     7981 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA
     8041 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA
     8101 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA
     8161 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT
     8221 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG
     8281 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC
     8341 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT
     8401 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA
     8461 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT
     8521 TTCAATATTA TTGAAGCATT TATCAGGGT
//