LOCUS       pAS_4xMmaPylT(UCA)_EF1_ 8550 bp ds-DNA     circular     05-OCT-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     772..791
                     /label="BII(R)"
     misc_feature    complement(834..846)
                     /label="3'TR"
     misc_feature    847..877
                     /label="31bp spacer"
     misc_feature    complement(847..877)
                     /label="3'TR"
     misc_feature    complement(878..879)
                     /label="3'TR"
     misc_feature    complement(881..896)
                     /label="3'TR"
     enhancer        1072..1303
                     /label="core insul"
     misc_feature    1325..1567
                     /label="h7SK promoter"
     misc_feature    1568..1636
                     /label="PylT TGA"
     misc_feature    1654..1896
                     /label="h7SK promoter"
     misc_feature    1897..1965
                     /label="PylT TGA"
     misc_feature    1983..2225
                     /label="h7SK promoter"
     misc_feature    2226..2294
                     /label="PylT TGA"
     misc_feature    2312..2554
                     /label="h7SK promoter"
     misc_feature    2555..2623
                     /label="PylT TGA"
     promoter        2656..3201
                     /label="EF1"
     misc_feature    3220..3943
                     /label="sfGFP 150TGA"
     CDS             3220..3943
                     /label="Translation 3220-3943"
     misc_feature    3948..4536
                     /label="IRES"
     misc_feature    4537..4545
                     /label="Kozak consensus sequences"
     CDS             4542..4950
                     /label="Translation 4542-4950"
     misc_feature    4554..4949
                     /label="Blasticidin Resistance Gene (bsd)"
     misc_feature    5075..5078
                     /label="SV40 pA"
     misc_feature    5084..5210
                     /label="SV40 pA"
     enhancer        complement(5459..5689)
                     /label="core insul"
     misc_feature    5964..5998
                     /label="5'TR"
     primer_bind     complement(6517..6536)
                     /label="pLac(F)"
     CDS             complement(7653..8510)
                     /label="Amp"
ORIGIN
        1 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT
       61 CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT
      121 TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA
      181 TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA
      241 AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG
      301 GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA
      361 AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG
      421 CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA
      481 GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG
      541 GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT
      601 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA
      661 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA
      721 TTCACGTTTT TGAACCCGTG GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG
      781 TGATGGAGCA GATGAAGATG CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA
      841 AAGATAATCA TATTGTGACG TACGTTAAAG ATAATCATGT GTAAAATTGA CGCATGTGTT
      901 TTATCGGTCT GTATATCGAG GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA
      961 TTTACACTTA CATACTAATA ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT
     1021 TAAAAAAAAC AAAAACTCAA AATTTCTTCT ATAAAGTAAC AAAACTTTTA TGAGGGACAG
     1081 CCCCCCCCCA AAGCCCCCAG GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG
     1141 AGCCGCCCGG GGCTCCGCTC CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG
     1201 CGGGGACAGC CCGGGCACGG GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG
     1261 CTGCTCTTTG AGCCTGCAGA CACCTGGGGG GATACGGGGA AAAGGCCTCC ACgGCCaact
     1321 agttctgcag tatttagcat gccccaccca tctgcaaggc attctggata gtgtcaaaac
     1381 agccggaaat caagtccgtt tatctcaaac tttagcattt tgggaataaa tgatatttgc
     1441 tatgctggtt aaattagatt ttagttaaat ttcctgctga agctctagta cgataagtaa
     1501 cttgacctaa gtgtaaagtt gagatttcct tcaggtttat atagcttgtg cgccgcctgg
     1561 gtacctcgga aacctgatca tgtagatcga acggactTCA aatccgttca gccgggttag
     1621 attcccgggg tttccggaca agtgcggttt ttcctgcagt atttagcatg ccccacccat
     1681 ctgcaaggca ttctggatag tgtcaaaaca gccggaaatc aagtccgttt atctcaaact
     1741 ttagcatttt gggaataaat gatatttgct atgctggtta aattagattt tagttaaatt
     1801 tcctgctgaa gctctagtac gataagtaac ttgacctaag tgtaaagttg agatttcctt
     1861 caggtttata tagcttgtgc gccgcctggg tacctcggaa acctgatcat gtagatcgaa
     1921 cggactTCAa atccgttcag ccgggttaga ttcccggggt ttccggacaa gtgcggtttt
     1981 tcctgcagta tttagcatgc cccacccatc tgcaaggcat tctggatagt gtcaaaacag
     2041 ccggaaatca agtccgttta tctcaaactt tagcattttg ggaataaatg atatttgcta
     2101 tgctggttaa attagatttt agttaaattt cctgctgaag ctctagtacg ataagtaact
     2161 tgacctaagt gtaaagttga gatttccttc aggtttatat agcttgtgcg ccgcctgggt
     2221 acctcggaaa cctgatcatg tagatcgaac ggactTCAaa tccgttcagc cgggttagat
     2281 tcccggggtt tccggacaag tgcggttttt cctgcagtat ttagcatgcc ccacccatct
     2341 gcaaggcatt ctggatagtg tcaaaacagc cggaaatcaa gtccgtttat ctcaaacttt
     2401 agcattttgg gaataaatga tatttgctat gctggttaaa ttagatttta gttaaatttc
     2461 ctgctgaagc tctagtacga taagtaactt gacctaagtg taaagttgag atttccttca
     2521 ggtttatata gcttgtgcgc cgcctgggta cctcggaaac ctgatcatgt agatcgaacg
     2581 gactTCAaat ccgttcagcc gggttagatt cccggggttt ccggacaagt gcggtttttc
     2641 aactagtgct ctagaaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac
     2701 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag
     2761 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2821 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2881 gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg
     2941 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg
     3001 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg
     3061 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct
     3121 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc
     3181 caagctgtga ccggcgccta ctctagagCT AGCGCCACCA TGGTGTCCAA GGGCGAGGAA
     3241 CTGTTCACCG GCGTGGTGCC CATCCTGGTG GAACTGGATG GCGACGTGAA CGGCCACAAG
     3301 TTCTCTGTGC GGGGAGAGGG CGAAGGCGAC GCCACAAATG GCAAGCTGAC CCTGAAGTTC
     3361 ATCTGCACCA CCGGCAAGCT GCCCGTGCCT TGGCCTACCC TCGTGACCAC ACTGACCTAC
     3421 GGCGTGCAGT GCTTCAGCAG ATACCCCGAC CATATGAAGC GGCACGACTT CTTCAAGAGC
     3481 GCCATGCCCG AGGGCTACGT GCAGGAACGG ACCATCAGCT TCAAGGACGA CGGCACCTAC
     3541 AAGACCAGAG CCGAAGTGAA GTTCGAGGGC GACACCCTCG TGAACCGGAT CGAGCTGAAG
     3601 GGCATCGATT TCAAAGAGGA CGGCAACATC CTGGGCCACA AGCTGGAGTA CAACTTCAAC
     3661 AGCCACTGAG TGTACATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG
     3721 ATCCGGCACA ACGTGGAAGA TGGCAGCGTG CAGCTGGCCG ACCACTACCA GCAGAACACC
     3781 CCCATCGGAG ATGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGAGCGTG
     3841 CTGAGCAAGG ACCCCAACGA GAAGCGGGAC CACATGGTGC TGCTGGAATT CGTGACCGCC
     3901 GCTGGCATCA CCCACGGCAT GGACGAGCTG TACAAGGGCA GCTAGgcGGC CGCgcccctc
     3961 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     4021 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     4081 tggccctgtc ttcttgacga gcattcctag gggtctttcc cCtctcgcca aaggaatgca
     4141 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     4201 gtctgTagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     4261 ccaaaagcca cgtgtataag atacacctgC aaaggcggca caaccccagt gccacgttgt
     4321 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaAggggct
     4381 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     4441 ctttacatgt gtttagtCga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     4501 ggttttcctt tgaaaaaCAC GATGATAATA TGGCCACAAC CATGgcgtcc ggaATGGCCA
     4561 AGCCTTTGTC TCAAGAAGAA TCCACCCTCA TTGAAAGAGC AACGGCTACA ATCAACAGCA
     4621 TCCCCATCTC TGAAGACTAC AGCGTCGCCA GCGCAGCTCT CTCTAGCGAC GGCCGCATCT
     4681 TCACTGGTGT CAATGTATAT CATTTTACTG GGGGACCTTG TGCAGAACTC GTGGTGCTGG
     4741 GCACTGCTGC TGCTGCGGCA GCTGGCAACC TGACTTGTAT CGTCGCGATC GGAAATGAGA
     4801 ACAGGGGCAT CTTGAGCCCC TGCGGACGGT GCCGACAGGT GCTTCTCGAT CTGCATCCTG
     4861 GGATCAAAGC CATAGTGAAG GACAGTGATG GACAGCCGAC GGCAGTTGGG ATTCGTGAAT
     4921 TGCTGCCCTC TGGTTATGTG TGGGAGGGCT GACCATGggt cgacaatcaa cctctggatt
     4981 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     5041 gatacgctgc tttaatgcct ttgtatcatg cGTTAACTaa actTGTTTAT TGCAGCTTAT
     5101 AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG
     5161 CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GAATTGACTC
     5221 AAATGATGTC AATTAGTCTA TCAGAAGCTC ATCTGGTCTC CCTTCCGGGG GACAAGACAT
     5281 CCCTGTTTAA TATTTAAACA GCAGTGTTCC CAAACTGGGT TCTTATATCC CTTGCTCTGG
     5341 TCAACCAGGT TGCAGGGTTT CCTGTCCTCA CAGGAACGAA GTCCCTAAAG AAACAGTGGC
     5401 AGCCAGGTTT AGCCCCGGAA TTGACTGGAT TCCTTTTTTA GGGCCCATTG GTATGGCTTT
     5461 TTCCCCGTAT CCCCCCAGGT GTCTGCAGGC TCAAAGAGCA GCGAGAAGCG TTCAGAGGAA
     5521 AGCGATCCCG TGCCACCTTC CCCGTGCCCG GGCTGTCCCC GCACGCTGCC GGCTCGGGGA
     5581 TGCGGGGGGA GCGCCGGACC GGAGCGGAGC CCCGGGCGGC TCGCTGCTGC CCCCTAGCGG
     5641 GGGAGGGACG TAATTACATC CCTGGGGGCT TTGGGGGGGG GCTGTCCCTG ATATCTATAA
     5701 CAAGAAAATA TATATATAAT AAGTTATCAC GTAAGTAGAA CATGAAATAA CAATATAATT
     5761 ATCGTATGAG TTAAATCTTA AAAGTCACGT AAAAGATAAT CATGCGTCAT TTTGACTCAC
     5821 GCGGTCGTTA TAGTTCAAAA TCAGTGACAC TTACCGCATT GACAAGCACG CCTCACGGGA
     5881 GCTCCAAGCG GCGACTGAGA TGTCCTAAAT GCACAGCGAC GGATTCGCGC TATTTAGAAA
     5941 GAGAGAGCAA TATTTCAAGA ATGCATGCGT CAATTTTACG CAGACTATCT TTCTAGGGTT
     6001 AATCTAGCTG CATCAGGATC ATATCGTCGG GTCTTTTTTC CGGCTCAGTC ATCGCCCAAG
     6061 CTGGCGCTAT CTGGGCATCG GGGAGGAAGA AGCCCGTGCC TTTTCCCGCG AGGTTGAAGC
     6121 GGCATGGAAA GAGTTTGCCG AGGATGACTG CTGCTGCATT GACGTTGAGC GAAAACGCAC
     6181 GTTTACCATG ATGATTCGGG AAGGTGTGGC CATGCACGCC TTTAACGGTG AACTGTTCGT
     6241 TCAGGCCACC TGGGATACCA GTTCGTCGCG GCTTTTCCGG ACACAGTTCC GGATGGTCAG
     6301 CCCGAAGCGC ATCAGCAACC CGAACAATAC CGGCGACAGC CGGAACTGCC GTGCCGGTGT
     6361 GCAGATTAAT GACAGCGGTG CGGCGCTGGG ATATTACGTC AGCGAGGACG GGTATCCTGG
     6421 CTGGATGCCG CAGAAATGGA CATGGATACC CCGTGAGTTA CCCGGCGGGC GCGCTTGGCG
     6481 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC
     6541 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA
     6601 TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT
     6661 TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC
     6721 TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA
     6781 AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA
     6841 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG
     6901 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG
     6961 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT
     7021 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT
     7081 TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC
     7141 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
     7201 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT
     7261 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC
     7321 TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA
     7381 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT
     7441 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT
     7501 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     7561 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     7621 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     7681 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     7741 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC
     7801 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     7861 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     7921 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     7981 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     8041 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     8101 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     8161 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     8221 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     8281 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     8341 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     8401 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     8461 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     8521 TTTCAATATT ATTGAAGCAT TTATCAGGGT
//