LOCUS       Exported                9828 bp ds-DNA    circular SYN 12-AUG-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pPB-MPP8_Flag2-Blast
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9828)
  AUTHORS   1234
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2021 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9828
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             2208..2520
                     /note="PB 3'LTR"
     misc_feature    2243..2262
                     /note="PB_RP1"
     misc_feature    complement(2496..2520)
                     /note="PB_FP1"
     misc_feature    2521..2554
                     /note="LoxP"
     enhancer        2566..2944
                     /note="CMV IE"
                     /note="CMV IE (human cytomegalovirus immediate early
                     enhancer)"
     promoter        2946..3221
                     /note="actin promoter"
     exon            3222..3311
                     /note="chicken beta-actin splice donor from"
                     /note="chicken beta-actin splice donor from exon 1"
     intron          3312..4230
                     /note="chimeric intron"
     intron          3312..4180
                     /note="b-actin intron 1"
                     /note="b-actin intron 1 (lacking 33 most 3' nts)"
     intron          4190..4230
                     /note="rabbit beta-globin intron (3' part of)"
     exon            4232..4284
                     /note="rabbit beta-globin 3rd exon (5' part of)"
     misc_recomb     4295..4308
                     /note="attR1"
     protein_bind    4309..4318
                     /gene="mutant version of attL"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attL1"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    4333..4338
                     /note="Kozak"
     misc_feature    4342..6912
                     /note="MPP8"
     CDS             6919..6942
                     /codon_start=1
                     /note="Flag1"
                     /translation="DYKDDDDK"
     misc_feature    6943..6966
                     /note="Flag2"
     protein_bind    complement(6992..7001)
                     /gene="mutant version of attL"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attL2"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     7002..7016
                     /note="attR2"
     misc_feature    7236..7316
                     /note="IRES"
     misc_feature    7317..7734
                     /note="IRES"
     gene            7735..8139
                     /note="BSDR"
     polyA_site      8232..8683
                     /note="rabbit beta globin pA"
     polyA_site      8851..8879
                     /note="SV40 ori/pA"
                     /note="SV40 ori/pA (not sure the precise positions)"
     misc_feature    8899..8932
                     /note="LoxP"
     LTR             8935..9169
                     /note="PB 5'LTR"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtacctta accctagaaa
     2221 gatagtctgc gtaaaattga cgcatgcatt cttgaaatat tgctctctct ttctaaatag
     2281 cgcgaatccg tcgctgtgca tttaggacat ctcagtcgcc gcttggagct cccgtgaggc
     2341 gtgcttgtca atgcggtaag tgtcactgat tttgaactat aacgaccgcg tgagtcaaaa
     2401 tgacgcatga ttatctttta cgtgactttt aagatttaac tcatacgata attatattgt
     2461 tatttcatgt tctacttacg tgataactta ttatatatat attttcttgt tatagatatc
     2521 ataacttcgt ataatgtatg ctatacgaag ttatgcggcc ctcgacattg attattgact
     2581 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     2641 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     2701 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     2761 tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     2821 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     2881 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     2941 atgggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
     3001 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
     3061 gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag
     3121 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
     3181 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgttgcctt
     3241 cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
     3301 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
     3361 tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag ccttaaaggg ctccgggagg
     3421 gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     3481 gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
     3541 gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     3601 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
     3661 gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc cctccccgag ttgctgagca
     3721 cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc gcggggctcg ccgtgccggg
     3781 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     3841 ctcgggggag gggcgcggcg gccccggagc gccggcggct gtcgaggcgc ggcgagccgc
     3901 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     3961 tggcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc gggcgaagcg
     4021 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     4081 tccccttctc catctccagc ctcggggctg ccgcaggggg acggctgcct tcggggggga
     4141 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
     4201 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tgttgtgctg
     4261 tctcatcatt ttggcaaaga attcctcgaa tcaacaagtt tgtacaaaaa agcaggctcc
     4321 gaattcgccc ttgccaccat ggcggcggcg gctgaggagg gcatgagtgc ggccgctcta
     4381 gttatgtcag ttcccgacag cattggccgt tctcccgagt ccgaaggagt tggagcaggg
     4441 gacgaagaga aggacgcagc caccaaagga acggtggccg ttggggacag cgaggaggat
     4501 ggggaggacg ttttcgaggt ggagaggatc ctggacatga agtgcgaggg aggtaagaat
     4561 ctttataaag ttcgatggaa aggatataca tctgaagatg atacctggga gcctgaggtt
     4621 catctggagg actgtaagga agttcttctt gaattcagga agaaacttgc tgagaacaaa
     4681 gctaaagcag tcaggaaaga tattcagaga ctatctttaa acaatgacat atttgaggca
     4741 gacagtgata gcgaccagca aagtgacaca aaagaagata tttcaccaag gaagaaaaag
     4801 aaaaaaataa agtgcaaaga agagacaagt ccagaagatc tgaggaaaaa aaggacaaaa
     4861 atgggaaaac taaaagacaa gttcaagaca gagctggaga gcacctctga aataatcggt
     4921 ttcgatgtaa agacgaagaa aagaatttgg gaagtcaagg aagaattaaa ggactccaaa
     4981 aagcccaaaa aggatgaaat aaaggaaacc aaggaattaa agaaagctaa taaaagggct
     5041 gaagtaagag atttaaagat taaaataaga gaagatgtca aagagaaccg aaaaacaaaa
     5101 aaagagagat atatagaatc tccgctggag tctgaatcgc ctaatgattc cctcattcta
     5161 gaggatgaca gtgaagactt tatttctgac aacagagaag agaatcagaa tgtgagaagt
     5221 gtaagagata agacagcaca ggagacagtg caagagggta tttttgagaa acatctggat
     5281 gaccttataa gtattgaaga ggatgctggt acccgagtta gaaggaagaa gacgaagcca
     5341 agaaagtttg aggaaccaaa agagatcaag aagcttgaaa gcacgaatgc ctttttagaa
     5401 aggagagcaa tacccaaaaa gcaaagaaac caagacaagg gcataagtaa cctagagtta
     5461 aataagctac catcacctgt gtttgcccaa accctgaaaa gttccagact gagtggggaa
     5521 gagaagagcc taaagtcccc tgacttggca gaagaggaga aagagaaaaa aaatgaaccc
     5581 aaaggaaaat accagaaaag gtatgatttg gacaaagaag aaaaagccag aaaagagcca
     5641 aaggtattaa aatcatttaa ggaaatcaga aatgcatttg atttatttaa aaaaacaaca
     5701 gaagaaaaaa atgatgttct tgagaataat tcaaaaagag aagaaatatc actggattct
     5761 aaaattatga atgataacaa aaccaaggac aagtgttcac ttaaagaaaa aagaaatact
     5821 cgagatgaga ctgacacttg ggcatacatt gctgcagaag gggatcagga agtttcagac
     5881 agtgtatgcc aaacagatga gacgtctgat ggcaggcaac cagttttgag tttgggtatg
     5941 gacctgcagc tggagtggat gaaattagaa gattttcaga agcatctgga tggggaagat
     6001 gagcccttta tcacaacaaa caggattcca aataatttgt tgagggatgc tgtgaaaaat
     6061 ggagattata ttgctgtgaa ggttgcactg aactcaaatg aagaatacaa cttggatcaa
     6121 gaggattcta ctgggatgac cctggtgatg ttggctgcag caggcggtca ggacgacctc
     6181 ctcagactcc ttatcaccaa aggtgcaaaa gtgaatgggc gccagaaaaa cgggactaca
     6241 gccctcattc atgccgctga gaagaacttt ttaacaactg tagctattct tttggaagct
     6301 ggagcttttg tgaatgttca gcaaagcaat ggtgagactg cgctcatgaa agcatgcaaa
     6361 agaggaaatt cggacattgt ccgccttgtg attgaatgtg gagctgactg caacattttg
     6421 tcaaagcacc aaaatagtgc attgtacttt gcaaagcagt gtaataatgt gcttgtatat
     6481 gaactgctga agagccactt ggagacactt tccagagttg cagaagaaac aatcagggat
     6541 tactttgaat ctcgccttgc tctactggaa cctgttttcc caatagcatg ccatcggctc
     6601 tgtgaggggc cggatttctc aacagatttc aattacatgc cccctcagaa catgcctgaa
     6661 ggctctggtg tcctcctctt tattttccat gcaaactttt tgggcaaaga cgttattgct
     6721 cggctttgtg gaccatgcag tgtacaagct gtggttttaa atgataaatt tcaacttcct
     6781 gtttttctgg acagtcactt tgtttattca ttcagtcctg ttgctggccc caataagttg
     6841 tttataaggt tgacggaagc accctttgcc aaggttaagt tgctaatagg tgcatacaga
     6901 gtacagctgc agggcgccga ctacaaggac gacgatgaca aggactacaa ggacgacgat
     6961 gacaagcgta ccggttagaa gggcgaattc gacccagctt tcttgtacaa agtggttgat
     7021 tcgaggccgc tcgataagct tgatatcgaa ttcactcctc aggtgcaggc tgcctatcag
     7081 aaggtggtgg ctggtgtggc caactggctc acaaatacca ctgagatcga cggtatcgat
     7141 aagcttgata tcgaattccg cccctctccc tccccccccc ctaacgttac tggccgaagc
     7201 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     7261 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     7321 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     7381 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     7441 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     7501 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     7561 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     7621 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc
     7681 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     7741 acaaccatgc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
     7801 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
     7861 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
     7921 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
     7981 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
     8041 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
     8101 cgtgaattgc tgccctctgg ttatgtgtgg gagggttaac tagataagta atgatcataa
     8161 tcagccatat cacatctgta gaggttttac ttgctttgtc cgacggcggc ccacgggtcc
     8221 caggggggtc gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     8281 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     8341 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     8401 gtatttggtt tagagtttgg caacatatgc catatgctgg ctgccatgaa caaaggtggc
     8461 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     8521 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     8581 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     8641 tcccagtcat agctgtccct cttctcttat gaagatccct cgacctgcag cccaagcttg
     8701 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8761 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     8821 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     8881 atccgcggcc gcgtcgagat aacttcgtat aatgtatgct atacgaagtt atgttttgtt
     8941 actttataga agaaattttg agtttttgtt tttttttaat aaataaataa acataaataa
     9001 attgtttgtt gaatttatta ttagtatgta agtgtaaata taataaaact taatatctat
     9061 tcaaattaat aaataaacct cgatatacag accgataaaa cacatgcgtc aattttacgc
     9121 atgattatct ttaacgtacg tcacaatatg attatctttc tagggttaaa gagctccaat
     9181 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     9241 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     9301 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     9361 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     9421 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     9481 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     9541 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     9601 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     9661 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     9721 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     9781 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttag
//
