LOCUS       Exported                9498 bp ds-DNA    circular SYN 12-AUG-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pPB-MPP8-112–858_Flag2-Blast
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9498)
  AUTHORS   1234
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2021 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9498
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             2208..2520
                     /note="PB 3'LTR"
     misc_feature    2243..2262
                     /note="PB_RP1"
     misc_feature    complement(2496..2520)
                     /note="PB_FP1"
     misc_feature    2521..2554
                     /note="LoxP"
     enhancer        2566..2944
                     /note="CMV IE"
                     /note="CMV IE (human cytomegalovirus immediate early 
                     enhancer)"
     promoter        2946..3221
                     /note="actin promoter"
     exon            3222..3311
                     /note="chicken beta-actin splice donor from"
                     /note="chicken beta-actin splice donor from exon 1"
     intron          3312..4230
                     /note="chimeric intron"
     intron          3312..4180
                     /note="b-actin intron 1"
                     /note="b-actin intron 1 (lacking 33 most 3' nts)"
     intron          4190..4230
                     /note="rabbit beta-globin intron (3' part of)"
     exon            4232..4284
                     /note="rabbit beta-globin 3rd exon (5' part of)"
     misc_recomb     4295..4308
                     /note="attR1"
     protein_bind    4309..4318
                     /gene="mutant version of attL"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attL1"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    4333..4338
                     /note="Kozak"
     misc_feature    4342..6582
                     /note="MPP8 112–858"
     CDS             6589..6612
                     /codon_start=1
                     /note="Flag1"
                     /translation="DYKDDDDK"
     misc_feature    6613..6636
                     /note="Flag2"
     protein_bind    complement(6662..6671)
                     /gene="mutant version of attL"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attL2"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     6672..6686
                     /note="attR2"
     misc_feature    6906..6986
                     /note="IRES"
     misc_feature    6987..7404
                     /note="IRES"
     gene            7405..7809
                     /note="BSDR"
     polyA_site      7902..8353
                     /note="rabbit beta globin pA"
     polyA_site      8521..8549
                     /note="SV40 ori/pA"
                     /note="SV40 ori/pA (not sure the precise positions)"
     misc_feature    8569..8602
                     /note="LoxP"
     LTR             8605..8839
                     /note="PB 5'LTR"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtacctta accctagaaa
     2221 gatagtctgc gtaaaattga cgcatgcatt cttgaaatat tgctctctct ttctaaatag
     2281 cgcgaatccg tcgctgtgca tttaggacat ctcagtcgcc gcttggagct cccgtgaggc
     2341 gtgcttgtca atgcggtaag tgtcactgat tttgaactat aacgaccgcg tgagtcaaaa
     2401 tgacgcatga ttatctttta cgtgactttt aagatttaac tcatacgata attatattgt
     2461 tatttcatgt tctacttacg tgataactta ttatatatat attttcttgt tatagatatc
     2521 ataacttcgt ataatgtatg ctatacgaag ttatgcggcc ctcgacattg attattgact
     2581 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     2641 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     2701 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     2761 tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     2821 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     2881 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     2941 atgggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
     3001 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
     3061 gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag
     3121 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
     3181 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgttgcctt
     3241 cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
     3301 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
     3361 tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag ccttaaaggg ctccgggagg
     3421 gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     3481 gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
     3541 gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     3601 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
     3661 gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc cctccccgag ttgctgagca
     3721 cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc gcggggctcg ccgtgccggg
     3781 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     3841 ctcgggggag gggcgcggcg gccccggagc gccggcggct gtcgaggcgc ggcgagccgc
     3901 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     3961 tggcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc gggcgaagcg
     4021 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     4081 tccccttctc catctccagc ctcggggctg ccgcaggggg acggctgcct tcggggggga
     4141 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
     4201 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tgttgtgctg
     4261 tctcatcatt ttggcaaaga attcctcgaa tcaacaagtt tgtacaaaaa agcaggctcc
     4321 gaattcgccc ttgccaccat ggagaacaaa gctaaagcag tcaggaaaga tattcagaga
     4381 ctatctttaa acaatgacat atttgaggca gacagtgata gcgaccagca aagtgacaca
     4441 aaagaagata tttcaccaag gaagaaaaag aaaaaaataa agtgcaaaga agagacaagt
     4501 ccagaagatc tgaggaaaaa aaggacaaaa atgggaaaac taaaagacaa gttcaagaca
     4561 gagctggaga gcacctctga aataatcggt ttcgatgtaa agacgaagaa aagaatttgg
     4621 gaagtcaagg aagaattaaa ggactccaaa aagcccaaaa aggatgaaat aaaggaaacc
     4681 aaggaattaa agaaagctaa taaaagggct gaagtaagag atttaaagat taaaataaga
     4741 gaagatgtca aagagaaccg aaaaacaaaa aaagagagat atatagaatc tccgctggag
     4801 tctgaatcgc ctaatgattc cctcattcta gaggatgaca gtgaagactt tatttctgac
     4861 aacagagaag agaatcagaa tgtgagaagt gtaagagata agacagcaca ggagacagtg
     4921 caagagggta tttttgagaa acatctggat gaccttataa gtattgaaga ggatgctggt
     4981 acccgagtta gaaggaagaa gacgaagcca agaaagtttg aggaaccaaa agagatcaag
     5041 aagcttgaaa gcacgaatgc ctttttagaa aggagagcaa tacccaaaaa gcaaagaaac
     5101 caagacaagg gcataagtaa cctagagtta aataagctac catcacctgt gtttgcccaa
     5161 accctgaaaa gttccagact gagtggggaa gagaagagcc taaagtcccc tgacttggca
     5221 gaagaggaga aagagaaaaa aaatgaaccc aaaggaaaat accagaaaag gtatgatttg
     5281 gacaaagaag aaaaagccag aaaagagcca aaggtattaa aatcatttaa ggaaatcaga
     5341 aatgcatttg atttatttaa aaaaacaaca gaagaaaaaa atgatgttct tgagaataat
     5401 tcaaaaagag aagaaatatc actggattct aaaattatga atgataacaa aaccaaggac
     5461 aagtgttcac ttaaagaaaa aagaaatact cgagatgaga ctgacacttg ggcatacatt
     5521 gctgcagaag gggatcagga agtttcagac agtgtatgcc aaacagatga gacgtctgat
     5581 ggcaggcaac cagttttgag tttgggtatg gacctgcagc tggagtggat gaaattagaa
     5641 gattttcaga agcatctgga tggggaagat gagcccttta tcacaacaaa caggattcca
     5701 aataatttgt tgagggatgc tgtgaaaaat ggagattata ttgctgtgaa ggttgcactg
     5761 aactcaaatg aagaatacaa cttggatcaa gaggattcta ctgggatgac cctggtgatg
     5821 ttggctgcag caggcggtca ggacgacctc ctcagactcc ttatcaccaa aggtgcaaaa
     5881 gtgaatgggc gccagaaaaa cgggactaca gccctcattc atgccgctga gaagaacttt
     5941 ttaacaactg tagctattct tttggaagct ggagcttttg tgaatgttca gcaaagcaat
     6001 ggtgagactg cgctcatgaa agcatgcaaa agaggaaatt cggacattgt ccgccttgtg
     6061 attgaatgtg gagctgactg caacattttg tcaaagcacc aaaatagtgc attgtacttt
     6121 gcaaagcagt gtaataatgt gcttgtatat gaactgctga agagccactt ggagacactt
     6181 tccagagttg cagaagaaac aatcagggat tactttgaat ctcgccttgc tctactggaa
     6241 cctgttttcc caatagcatg ccatcggctc tgtgaggggc cggatttctc aacagatttc
     6301 aattacatgc cccctcagaa catgcctgaa ggctctggtg tcctcctctt tattttccat
     6361 gcaaactttt tgggcaaaga cgttattgct cggctttgtg gaccatgcag tgtacaagct
     6421 gtggttttaa atgataaatt tcaacttcct gtttttctgg acagtcactt tgtttattca
     6481 ttcagtcctg ttgctggccc caataagttg tttataaggt tgacggaagc accctttgcc
     6541 aaggttaagt tgctaatagg tgcatacaga gtacagctgc agggcgccga ctacaaggac
     6601 gacgatgaca aggactacaa ggacgacgat gacaagcgta ccggttagaa gggcgaattc
     6661 gacccagctt tcttgtacaa agtggttgat tcgaggccgc tcgataagct tgatatcgaa
     6721 ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caactggctc
     6781 acaaatacca ctgagatcga cggtatcgat aagcttgata tcgaattccg cccctctccc
     6841 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     6901 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     6961 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     7021 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     7081 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     7141 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     7201 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     7261 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     7321 acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     7381 tcctttgaaa aacacgatga taatatggcc acaaccatgc ctttgtctca agaagaatcc
     7441 accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc
     7501 gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat
     7561 tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct
     7621 ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc
     7681 ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac
     7741 agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg
     7801 gagggttaac tagataagta atgatcataa tcagccatat cacatctgta gaggttttac
     7861 ttgctttgtc cgacggcggc ccacgggtcc caggggggtc gatctttttc cctctgccaa
     7921 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     7981 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     8041 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     8101 catatgctgg ctgccatgaa caaaggtggc tataaagagg tcatcagtat atgaaacagc
     8161 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     8221 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     8281 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     8341 gaagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     8401 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     8461 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     8521 ttccagtcgg gaaacctgtc gtgccagcgg atccgcggcc gcgtcgagat aacttcgtat
     8581 aatgtatgct atacgaagtt atgttttgtt actttataga agaaattttg agtttttgtt
     8641 tttttttaat aaataaataa acataaataa attgtttgtt gaatttatta ttagtatgta
     8701 agtgtaaata taataaaact taatatctat tcaaattaat aaataaacct cgatatacag
     8761 accgataaaa cacatgcgtc aattttacgc atgattatct ttaacgtacg tcacaatatg
     8821 attatctttc tagggttaaa gagctccaat tcgccctata gtgagtcgta ttacgcgcgc
     8881 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     8941 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     9001 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     9061 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     9121 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     9181 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     9241 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     9301 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     9361 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     9421 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     9481 ttaacgctta caatttag
//
