LOCUS       pCDF_hRFC1deltaN55        5344 bp ds-DNA   circular     10-AUG-2021
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     17..36
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    104..109
                     /label=linker
                     /ApEinfo_fwdcolor=#0060ff
                     /ApEinfo_revcolor=green
     misc_feature    104..1888
                     /label=hRFC1-delta555Nter
                     /ApEinfo_fwdcolor=#00e6ff
                     /ApEinfo_revcolor=green
     misc_binding    36..58
                     /label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
     CDS             complement(4158..5114)
                     /label=LacI
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
ORIGIN
        1 tcctgcatta ggaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc
       61 cctgtagaaa taattttgtt taactttaat aaggagatat accATGGACA AAGAACAGGT
      121 TGCCGAAGAA ACGTCTGGTG ATAGCAAAGC TCGTAATCTG GCGGATGATT CCAGCGAAAA
      181 TAAAGTTGAA AACTTGCTGT GGGTCGATAA ATATAAACCG ACCTCTCTGA AAACGATTAT
      241 CGGCCAGCAG GGCGACCAGT CTTGCGCCAA CAAACTGCTG CGCTGGCTGC GTAATTGGCA
      301 GAAAAGCTCG AGCGAAGATA AAAAACATGC CAAATTTGGC AAATTTAGCG GTAAAGACGA
      361 CGGCTCTTCC TTTAAAGCGG CTCTGCTGAG CGGTCCGCCG GGTGTTGGTA AAACCACCAC
      421 CGCCTCGCTG GTTTGCCAGG AACTGGGTTA CTCCTATGTG GAACTGAACG CGAGCGATAC
      481 GCGCAGCAAA TCCTCTCTGA AAGCAATTGT TGCTGAAAGC CTGAATAACA CGAGCATCAA
      541 AGGTTTTTAT TCTAACGGCG CAGCGTCTTC TGTGTCTACC AAACATGCCC TGATTATGGA
      601 CGAAGTGGAT GGCATGGCAG GCAATGAAGA TCGTGGTGGT ATCCAGGAAC TGATCGGTCT
      661 GATCAAACAT ACCAAAATTC CGATTATCTG TATGTGCAAC GATCGTAACC ACCCGAAAAT
      721 TCGTAGCCTG GTTCATTATT GCTTTGATCT GCGCTTTCAA CGTCCGCGTG TTGAACAGAT
      781 TAAAGGCGCA ATGATGTCCA TTGCCTTTAA AGAAGGTCTG AAAATTCCGC CGCCGGCGAT
      841 GAATGAAATT ATTCTGGGCG CCAACCAGGA TATTCGTCAA GTGCTGCATA ATCTGTCCAT
      901 GTGGTGTGCC CGCTCCAAAG CGCTGACCTA CGACCAGGCA AAAGCCGACT CACATCGTGC
      961 AAAGAAAGAC ATTAAAATGG GCCCGTTTGA TGTTGCGCGC AAAGTGTTCG CCGCGGGTGA
     1021 AGAAACCGCG CACATGTCGC TGGTGGATAA AAGCGATCTG TTCTTCCATG ATTATAGTAT
     1081 TGCACCGCTG TTCGTTCAAG AAAATTACAT TCATGTGAAA CCGGTTGCCG CCGGTGGCGA
     1141 TATGAAAAAA CATCTGATGC TGCTGTCGCG TGCGGCCGAT AGTATCTGTG ATGGTGATCT
     1201 GGTGGATTCA CAGATTCGTT CCAAACAGAA TTGGTCACTG CTGCCGGCCC AGGCGATTTA
     1261 TGCATCTGTG CTGCCGGGTG AACTGATGCG CGGTTATATG ACGCAGTTTC CGACGTTTCC
     1321 GAGTTGGCTG GGCAAACATT CCAGCACCGG TAAACATGAC CGTATCGTCC AAGACCTGGC
     1381 GCTGCACATG TCCCTGCGCA CCTACTCTTC CAAACGCACC GTCAATATGG ACTACCTGTC
     1441 CCTGCTGCGT GATGCACTGG TCCAGCCGCT GACGTCCCAA GGCGTCGACG GCGTGCAGGA
     1501 TGTTGTCGCG CTGATGGATA CCTATTACCT GATGAAAGAA GACTTCGAAA ACATTATGGA
     1561 AATTTCTTCT TGGGGTGGCA AACCGTCGCC GTTCAGTAAA CTGGACCCGA AAGTGAAAGC
     1621 CGCGTTTACC CGCGCATATA ATAAAGAAGC ACACCTGACC CCGTACTCTC TGCAGGCAAT
     1681 CAAAGCGTCC CGTCATTCGA CGTCTCCGTC TCTGGATTCC GAATATAACG AAGAACTGAA
     1741 TGAAGACGAT AGCCAGAGCG ATGAAAAAGA CCAAGATGCT ATTGAAACCG ACGCGATGAT
     1801 CAAGAAAAAG ACCAAAAGCT CCAAACCGTC TAAACCGGAA AAAGATAAAG AACCGCGTAA
     1861 AGGTAAAGGT AAAAGCTCGA AAAAATAAGG ATCCCAATTG GGAGCTCGTG TACACGGCGC
     1921 GCCTGCAGGT CGACAAGCTT GCGGCCGCAC TCGAGTCTGG TAAAgaaacc gctgctgcga
     1981 aatttgaacg ccagcacatg gactcgtcta ctagcgcagc ttaattaacc taggctgctg
     2041 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
     2101 ttttgctgaa acctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata
     2161 aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga
     2221 ccgggtcatc gtggccggat cttgcggccc ctcggcttga acgaattgtt agacattatt
     2281 tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg
     2341 cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga
     2401 cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg
     2461 cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct
     2521 catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa
     2581 atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa
     2641 cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct
     2701 cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag
     2761 ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga
     2821 gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc
     2881 gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct
     2941 tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat
     3001 ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt
     3061 ccctcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3121 gcggatacat atttgaatgt atttagaaaa ataaacaaat agctagctca ctcggtcgct
     3181 acgctccggg cgtgagactg cggcgggcgc tgcggacaca tacaaagtta cccacagatt
     3241 ccgtggataa gcaggggact aacatgtgag gcaaaacagc agggccgcgc cggtggcgtt
     3301 tttccatagg ctccgccctc ctgccagagt tcacataaac agacgctttt ccggtgcatc
     3361 tgtgggagcc gtgaggctca accatgaatc tgacagtacg ggcgaaaccc gacaggactt
     3421 aaagatcccc accgtttccg gcgggtcgct ccctcttgcg ctctcctgtt ccgaccctgc
     3481 cgtttaccgg atacctgttc cgcctttctc ccttacggga agtgtggcgc tttctcatag
     3541 ctcacacact ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa
     3601 gaactccccg ttcagcccga ctgctgcgcc ttatccggta actgttcact tgagtccaac
     3661 ccggaaaagc acggtaaaac gccactggca gcagccattg gtaactggga gttcgcagag
     3721 gatttgttta gctaaacacg cggttgctct tgaagtgtgc gccaaagtcc ggctacactg
     3781 gaaggacaga tttggttgct gtgctctgcg aaagccagtt accacggtta agcagttccc
     3841 caactgactt aaccttcgat caaaccacct ccccaggtgg ttttttcgtt tacagggcaa
     3901 aagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tactgaaccg
     3961 ctctagattt cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga
     4021 tataagttgt aattctcatg ttagtcatgc cccgcgccca ccggaaggag ctgactgggt
     4081 tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat
     4141 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     4201 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt
     4261 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt
     4321 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt
     4381 aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatgtcc
     4441 gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg
     4501 ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga
     4561 aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga
     4621 gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc
     4681 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta
     4741 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat
     4801 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga
     4861 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag
     4921 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg
     4981 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca
     5041 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa
     5101 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc
     5161 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat
     5221 aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc
     5281 ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg
     5341 cgac     
//
