LOCUS       MS2_SC2_S_Alpha         6264 bp ds-DNA     circular     13-AUG-2021
DEFINITION  .
KEYWORDS    "Antibiotic Resistance"                                            
FEATURES             Location/Qualifiers
     misc_feature    464..464
                     /label="g->a (remove BspMI)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    710..710
                     /label="t->c (remove BsmBI)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1085..1474
                     /label="Coat Protein"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1085..1885
                     /label="coat protein dimer"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1420..1420
                     /label="c->t (remove BsaI)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1478..1480
                     /label="Start of second coat dimer"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1517..1534
                     /label="His Tag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1828..1828
                     /label="c->t (remove BsaI)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      1899..1960
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1961..2005
                     /label="Spacer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          2025..2050
                     /label="MS2 SC2 Variant GG rev"
                     /note="sequence: TCACGTCTCAATAGGAGAGTCCAACCAACAGAATCTATTGT"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             2025..3677
                     /label="Translation 2025-3677"
     misc_feature    2025..3678
                     /label="insert"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2571..2573
                     /label="N501Y"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2778..2780
                     /label="A570D"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3111..3113
                     /label="P681H"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    3216..3218
                     /label="T716I"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     terminator      3697..3758
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(3948..4630)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(4725..5522)
                     /label="KanR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        5964..5982
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     protein_bind    5983..6007
                     /label="lac operator"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        6016..6045
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     RBS             6065..6087
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             join(6144..6264,1..1061)
                     /label="Maturation Protein"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    6179..6179
                     /label="g->a (remove BsaI)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 ccggtcgttt taactcgact ggggccaaaa cgaaacagtg gcactacccc tctccgtatt
       61 cacggggggc gttaagtgtc acatcgatag atcaaggtgc ctacaagcga agtgggtcat
      121 cgtggggtcg cccgtacgag gagaaagccg gtttcggctt ctccctcgac gcacgctcct
      181 gctacagcct cttccctgta agccaaaact tgacttacat cgaagtgccg cagaacgttg
      241 cgaaccgggc gtcgaccgaa gtcctgcaaa aggtcaccca gggtaatttt aaccttggtg
      301 ttgctttagc agaggccagg tcgacagcct cacaactcgc gacgcaaacc attgcgctcg
      361 tgaaggcgta cactgccgct cgtcgcggta attggcgcca ggcgctccgc taccttgccc
      421 taaacgaaga tcgaaagttt cgatcaaaac acgtggccgg cagatggttg gagttgcagt
      481 tcggttggtt accactaatg agtgatatcc agggtgcata tgagatgctt acgaaggttc
      541 accttcaaga gtttcttcct atgagagccg tacgtcaggt cggtactaac atcaagttag
      601 atggccgtct gtcgtatcca gctgcaaact tccagacaac gtgcaacata tcgcgacgta
      661 tcgtgatatg gttttacata aacgatgcac gtttggcatg gttgtcgtcc ctaggtatct
      721 tgaacccact aggtatagtg tgggaaaagg tgcctttctc attcgttgtc gactggctcc
      781 tacctgtagg taacatgctc gagggcctta cggcccccgt gggatgctcc tacatgtcag
      841 gaacagttac tgacgtaata acgggtgagt ccatcataag cgttgacgct ccctacgggt
      901 ggactgtgga gagacagggc actgctaagg cccaaatctc agccatgcat cgaggggtac
      961 aatccgtatg gccaacaact ggcgcgtacg taaagtctcc tttctcgatg gtccatacct
     1021 tagatgcgtt agcattaatc aggcaacggc tctctagata gAGCCCTCAA CCGGAGTTTG
     1081 AAGCATGGCT TCTAACTTTA CTCAGTTCGT TCTCGTCGAC AATGGCGGAA CTGGCGACGT
     1141 GACTGTCGCC CCAAGCAACT TCGCTAACGG GGTCGCTGAA TGGATCAGCT CTAACTCGCG
     1201 TTCACAGGCT TACAAAGTAA CCTGTAGCGT TCGTCAGAGC TCTGCGCAGA ATCGCAAATA
     1261 CACCATCAAA GTCGAGGTGC CTAAAGTGGC AACCCAGACT GTTGGTGGTG TAGAGCTTCC
     1321 TGTAGCCGCA TGGCGTTCGT ACTTAAATAT GGAACTAACC ATTCCAATTT TCGCTACGAA
     1381 TTCCGACTGC GAGCTTATTG TTAAGGCAAT GCAAGGTCTT CTAAAAGATG GAAACCCGAT
     1441 TCCCTCAGCA ATCGCAGCAA ACTCCGGCAT CTACGCTAAC TTTACTCAGT TCGTTCTCGT
     1501 CGACAATGGC GGTACCCATC ACCATCACCA TCACGGTACC GGCGACGTGA CTGTCGCCCC
     1561 AAGCAACTTC GCTAACGGGG TCGCTGAATG GATCAGCTCT AACTCGCGTT CACAGGCTTA
     1621 CAAAGTAACC TGTAGCGTtC GTCAGAGCTC TGCGCAGAAT CGCAAATACA CCATCAAAGT
     1681 CGAGGTGCCT AAAGTGGCAA CCCAGACTGT TGGTGGTGTA GAGCTTCCTG TAGCCGCATG
     1741 GCGTTCGTAC TTAAATATGG AACTAACCAT TCCAATTTTC GCTACGAATT CCGACTGCGA
     1801 GCTTATTGTT AAGGCAATGC AAGGTCTTCT AAAAGATGGA AACCCGATTC CCTCAGCAAT
     1861 CGCAGCAAAC TCCGGCATCT ACTAATAGAC GCCGGCCAcc gctgagcaat aactagcata
     1921 accccttggg gcctctaaac gggtcttgag gggttttttg TCGAACAGAA AGTAATCGTA
     1981 TTGTACACGG CCGCATAATC GAAATTAATA CGACTCACTA TAGGagagtc caaccaacag
     2041 aatctattgt tagatttcct aatattacaa acttgtgccc ttttggtgaa gtttttaacg
     2101 ccaccagatt tgcatctgtt tatgcttgga acaggaagag aatcagcaac tgtgttgctg
     2161 attattctgt cctatataat tccgcatcat tttccacttt taagtgttat ggagtgtctc
     2221 ctactaaatt aaatgatctc tgctttacta atgtctatgc agattcattt gtaattagag
     2281 gtgatgaagt cagacaaatc gctccagggc aaactggaaa gattgctgat tataattata
     2341 aattaccaga tgattttaca ggctgcgtta tagcttggaa ttctaacaat cttgattcta
     2401 aggttggtgg taattataat tacctgtata gattgtttag gaagtctaat ctcaaacctt
     2461 ttgagagaga tatttcaact gaaatctatc aggccggtag cacaccttgt aatggtgttg
     2521 aaggttttaa ttgttacttt cctttacaat catatggttt ccaacccact tatggtgttg
     2581 gttaccaacc atacagagta gtagtacttt cttttgaact tctacatgca ccagcaactg
     2641 tttgtggacc taaaaagtct actaatttgg ttaaaaacaa atgtgtcaat ttcaacttca
     2701 atggtttaac aggcacaggt gttcttactg agtctaacaa aaagtttctg cctttccaac
     2761 aatttggcag agacattgat gacactactg atgctgtccg tgatccacag acacttgaga
     2821 ttcttgacat tacaccatgt tcttttggtg gtgtcagtgt tataacacca ggaacaaata
     2881 cttctaacca ggttgctgtt ctttatcagg atgttaactg cacagaagtc cctgttgcta
     2941 ttcatgcaga tcaacttact cctacttggc gtgtttattc tacaggttct aatgtttttc
     3001 aaacacgtgc aggctgttta ataggggctg aacatgtcaa caactcatat gagtgtgaca
     3061 tacccattgg tgcaggtata tgcgctagtt atcagactca gactaattct catcggcggg
     3121 cacgtagtgt agctagtcaa tccatcattg cctacactat gtcacttggt gcagaaaatt
     3181 cagttgctta ctctaataac tctattgcca tacccataaa ttttactatt agtgttacca
     3241 cagaaattct accagtgtct atgaccaaga catcagtaga ttgtacaatg tacatttgtg
     3301 gtgattcaac tgaatgcagc aatcttttgt tgcaatatgg cagtttttgt acacaattaa
     3361 accgtgcttt aactggaata gctgttgaac aagacaaaaa cacccaagaa gtttttgcac
     3421 aagtcaaaca aatttacaaa acaccaccaa ttaaagattt tggtggtttt aatttttcac
     3481 aaatattacc agatccatca aaaccaagca agaggtcatt tattgaagat ctacttttca
     3541 acaaagtgac acttgcagat gctggcttca tcaaacaata tggtgattgc cttggtgata
     3601 ttgctgctag agacctcatt tgtgcacaaa agtttaacgg ccttactgtt ttgccacctt
     3661 tgctcacaga tgaaatgACA TGAGGATCAC CCATGTccgc tgagcaataa ctagcataac
     3721 cccttggggc ctctaaacgg gtcttgaggg gttttttgTA ATTTCACTGC CCGCTTTCCA
     3781 GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ACATGGTCAT AGCTGTTTCC TTGCGTATTG
     3841 GGCGCTCTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGG TAAAGCCTGG
     3901 GGTGCCTAAT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG
     3961 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG
     4021 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC
     4081 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG
     4141 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT
     4201 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC
     4261 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC
     4321 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG
     4381 TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA
     4441 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC
     4501 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT
     4561 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     4621 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     4681 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTATTA GAAAAATTCA
     4741 TCCAGCAGAC GATAAAACGC AATACGCTGG CTATCCGGTG CCGCAATGCC ATACAGCACC
     4801 AGAAAACGAT CCGCCCATTC GCCGCCCAGT TCTTCCGCAA TATCACGGGT GGCCAGCGCA
     4861 ATATCCTGAT AACGATCCGC CACGCCCAGA CGGCCGCAAT CAATAAAGCC GCTAAAACGG
     4921 CCATTTTCCA CCATAATGTT CGGCAGGCAC GCATCACCAT GGGTCACCAC CAGATCTTCG
     4981 CCATCCGGCA TGCTCGCTTT CAGACGCGCA AACAGCTCTG CCGGTGCCAG GCCCTGATGT
     5041 TCTTCATCCA GATCATCCTG ATCCACCAGG CCCGCTTCCA TACGGGTACG CGCACGTTCA
     5101 ATACGATGTT TCGCCTGATG ATCAAACGGA CAGGTCGCCG GGTCCAGGGT ATGCAGACGA
     5161 CGCATGGCAT CCGCCATAAT GCTCACTTTT TCTGCCGGCG CCAGATGGCT AGACAGCAGA
     5221 TCCTGACCCG GCACTTCGCC CAGCAGCAGC CAATCACGGC CCGCTTCGGT CACCACATCC
     5281 AGCACCGCCG CACACGGAAC ACCGGTGGTG GCCAGCCAGC TCAGACGCGC CGCTTCATCC
     5341 TGCAGCTCGT TCAGCGCACC GCTCAGATCG GTTTTCACAA ACAGCACCGG ACGACCCTGC
     5401 GCGCTCAGAC GAAACACCGC CGCATCAGAG CAGCCAATGG TCTGCTGCGC CCAATCATAG
     5461 CCAAACAGAC GTTCCACCCA CGCTGCCGGG CTACCCGCAT GCAGGCCATC CTGTTCAATC
     5521 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     5581 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA
     5641 AAAGTGCCAC CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT
     5701 AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG
     5761 AATAGACCGA GATAGGGTTG AGTGGCCGCT ACAGGGCGCT CCCATTCGCC ATTCAGGCTG
     5821 CGCAACTGTT GGGAAGGGCG TTTCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA
     5881 AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG
     5941 TTGTAAAACG ACGGCCAGTG AGGtaatacg actcactata ggggaattgt gagcggataa
     6001 caattcccct ctagatttac actttatgct tccggctcgt atgtttctag agaattcaaa
     6061 taattttgtt taactttaag aaggagattt aaatTGGCTA TCGCTGTAGG TAGCCGGAAT
     6121 TCCATTCCTA GGAGGTTTGA CCTgtgcgag cttttagtac ccttgatagg gagaacgaaa
     6181 ccttcgtccc ctccgttcgc gtttacgcgg acggtgagac tgaagataac tcattctctt
     6241 taaaatatcg ttcgaactgg actc
//