LOCUS       MS2_SC2_S_Lambda        6264 bp ds-DNA     circular     13-AUG-2021
DEFINITION  .
KEYWORDS    "Antibiotic Resistance"                                            
FEATURES             Location/Qualifiers
     RBS             47..69
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             126..1307
                     /label="Maturation Protein"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    161..161
                     /label="g->a (remove BsaI)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    710..710
                     /label="g->a (remove BspMI)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    956..956
                     /label="t->c (remove BsmBI)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1331..1720
                     /label="Coat Protein"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1331..2131
                     /label="coat protein dimer"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1666..1666
                     /label="c->t (remove BsaI)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1724..1726
                     /label="Start of second coat dimer"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1763..1780
                     /label="His Tag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2074..2074
                     /label="c->t (remove BsaI)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      2145..2206
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2207..2251
                     /label="Spacer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          2271..2296
                     /label="MS2 SC2 Variant GG rev"
                     /note="sequence: TCACGTCTCAATAGGAGAGTCCAACCAACAGAATCTATTGT"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2271..3924
                     /label="insert"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             2271..3924
                     /label="Translation 2271-3924"
     misc_feature    2670..2672
                     /label="L452Q"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2784..2786
                     /label="F490S"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3891..3893
                     /label="T859N"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(3900..3924)
                     /label="MS2 SC2 Variant GG fwd"
                     /note="sequence: CCTCGTCTCCATGTCATTTCATCTGTGAGCAAAGGTGG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      3943..4004
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(4194..4876)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(4971..5768)
                     /label="KanR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        6210..6228
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     protein_bind    6229..6253
                     /label="lac operator"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        join(6262..6264,1..27)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 acactttatg cttccggctc gtatgtttct agagaattca aataattttg tttaacttta
       61 agaaggagat ttaaatTGGC TATCGCTGTA GGTAGCCGGA ATTCCATTCC TAGGAGGTTT
      121 GACCTgtgcg agcttttagt acccttgata gggagaacga aaccttcgtc ccctccgttc
      181 gcgtttacgc ggacggtgag actgaagata actcattctc tttaaaatat cgttcgaact
      241 ggactcccgg tcgttttaac tcgactgggg ccaaaacgaa acagtggcac tacccctctc
      301 cgtattcacg gggggcgtta agtgtcacat cgatagatca aggtgcctac aagcgaagtg
      361 ggtcatcgtg gggtcgcccg tacgaggaga aagccggttt cggcttctcc ctcgacgcac
      421 gctcctgcta cagcctcttc cctgtaagcc aaaacttgac ttacatcgaa gtgccgcaga
      481 acgttgcgaa ccgggcgtcg accgaagtcc tgcaaaaggt cacccagggt aattttaacc
      541 ttggtgttgc tttagcagag gccaggtcga cagcctcaca actcgcgacg caaaccattg
      601 cgctcgtgaa ggcgtacact gccgctcgtc gcggtaattg gcgccaggcg ctccgctacc
      661 ttgccctaaa cgaagatcga aagtttcgat caaaacacgt ggccggcaga tggttggagt
      721 tgcagttcgg ttggttacca ctaatgagtg atatccaggg tgcatatgag atgcttacga
      781 aggttcacct tcaagagttt cttcctatga gagccgtacg tcaggtcggt actaacatca
      841 agttagatgg ccgtctgtcg tatccagctg caaacttcca gacaacgtgc aacatatcgc
      901 gacgtatcgt gatatggttt tacataaacg atgcacgttt ggcatggttg tcgtccctag
      961 gtatcttgaa cccactaggt atagtgtggg aaaaggtgcc tttctcattc gttgtcgact
     1021 ggctcctacc tgtaggtaac atgctcgagg gccttacggc ccccgtggga tgctcctaca
     1081 tgtcaggaac agttactgac gtaataacgg gtgagtccat cataagcgtt gacgctccct
     1141 acgggtggac tgtggagaga cagggcactg ctaaggccca aatctcagcc atgcatcgag
     1201 gggtacaatc cgtatggcca acaactggcg cgtacgtaaa gtctcctttc tcgatggtcc
     1261 ataccttaga tgcgttagca ttaatcaggc aacggctctc tagatagAGC CCTCAACCGG
     1321 AGTTTGAAGC ATGGCTTCTA ACTTTACTCA GTTCGTTCTC GTCGACAATG GCGGAACTGG
     1381 CGACGTGACT GTCGCCCCAA GCAACTTCGC TAACGGGGTC GCTGAATGGA TCAGCTCTAA
     1441 CTCGCGTTCA CAGGCTTACA AAGTAACCTG TAGCGTTCGT CAGAGCTCTG CGCAGAATCG
     1501 CAAATACACC ATCAAAGTCG AGGTGCCTAA AGTGGCAACC CAGACTGTTG GTGGTGTAGA
     1561 GCTTCCTGTA GCCGCATGGC GTTCGTACTT AAATATGGAA CTAACCATTC CAATTTTCGC
     1621 TACGAATTCC GACTGCGAGC TTATTGTTAA GGCAATGCAA GGTCTTCTAA AAGATGGAAA
     1681 CCCGATTCCC TCAGCAATCG CAGCAAACTC CGGCATCTAC GCTAACTTTA CTCAGTTCGT
     1741 TCTCGTCGAC AATGGCGGTA CCCATCACCA TCACCATCAC GGTACCGGCG ACGTGACTGT
     1801 CGCCCCAAGC AACTTCGCTA ACGGGGTCGC TGAATGGATC AGCTCTAACT CGCGTTCACA
     1861 GGCTTACAAA GTAACCTGTA GCGTtCGTCA GAGCTCTGCG CAGAATCGCA AATACACCAT
     1921 CAAAGTCGAG GTGCCTAAAG TGGCAACCCA GACTGTTGGT GGTGTAGAGC TTCCTGTAGC
     1981 CGCATGGCGT TCGTACTTAA ATATGGAACT AACCATTCCA ATTTTCGCTA CGAATTCCGA
     2041 CTGCGAGCTT ATTGTTAAGG CAATGCAAGG TCTTCTAAAA GATGGAAACC CGATTCCCTC
     2101 AGCAATCGCA GCAAACTCCG GCATCTACTA ATAGACGCCG GCCAccgctg agcaataact
     2161 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgTCGA ACAGAAAGTA
     2221 ATCGTATTGT ACACGGCCGC ATAATCGAAA TTAATACGAC TCACTATAGG agagtccaac
     2281 caacagaatc tattgttaga tttcctaata ttacaaactt gtgccctttt ggtgaagttt
     2341 ttaacgccac cagatttgca tctgtttatg cttggaacag gaagagaatc agcaactgtg
     2401 ttgctgatta ttctgtccta tataattccg catcattttc cacttttaag tgttatggag
     2461 tgtctcctac taaattaaat gatctctgct ttactaatgt ctatgcagat tcatttgtaa
     2521 ttagaggtga tgaagtcaga caaatcgctc cagggcaaac tggaaagatt gctgattata
     2581 attataaatt accagatgat tttacaggct gcgttatagc ttggaattct aacaatcttg
     2641 attctaaggt tggtggtaat tataattacc agtatagatt gtttaggaag tctaatctca
     2701 aaccttttga gagagatatt tcaactgaaa tctatcaggc cggtagcaca ccttgtaatg
     2761 gtgttgaagg ttttaattgt tactctcctt tacaatcata tggtttccaa cccactaatg
     2821 gtgttggtta ccaaccatac agagtagtag tactttcttt tgaacttcta catgcaccag
     2881 caactgtttg tggacctaaa aagtctacta atttggttaa aaacaaatgt gtcaatttca
     2941 acttcaatgg tttaacaggc acaggtgttc ttactgagtc taacaaaaag tttctgcctt
     3001 tccaacaatt tggcagagac attgctgaca ctactgatgc tgtccgtgat ccacagacac
     3061 ttgagattct tgacattaca ccatgttctt ttggtggtgt cagtgttata acaccaggaa
     3121 caaatacttc taaccaggtt gctgttcttt atcaggatgt taactgcaca gaagtccctg
     3181 ttgctattca tgcagatcaa cttactccta cttggcgtgt ttattctaca ggttctaatg
     3241 tttttcaaac acgtgcaggc tgtttaatag gggctgaaca tgtcaacaac tcatatgagt
     3301 gtgacatacc cattggtgca ggtatatgcg ctagttatca gactcagact aattctcctc
     3361 ggcgggcacg tagtgtagct agtcaatcca tcattgccta cactatgtca cttggtgcag
     3421 aaaattcagt tgcttactct aataactcta ttgccatacc cacaaatttt actattagtg
     3481 ttaccacaga aattctacca gtgtctatga ccaagacatc agtagattgt acaatgtaca
     3541 tttgtggtga ttcaactgaa tgcagcaatc ttttgttgca atatggcagt ttttgtacac
     3601 aattaaaccg tgctttaact ggaatagctg ttgaacaaga caaaaacacc caagaagttt
     3661 ttgcacaagt caaacaaatt tacaaaacac caccaattaa agattttggt ggttttaatt
     3721 tttcacaaat attaccagat ccatcaaaac caagcaagag gtcatttatt gaagatctac
     3781 ttttcaacaa agtgacactt gcagatgctg gcttcatcaa acaatatggt gattgccttg
     3841 gtgatattgc tgctagagac ctcatttgtg cacaaaagtt taacggcctt aatgttttgc
     3901 cacctttgct cacagatgaa atgaCATGAG GATCACCCAT GTccgctgag caataactag
     3961 cataacccct tggggcctct aaacgggtct tgaggggttt tttgTAATTT CACTGCCCGC
     4021 TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAACAT GGTCATAGCT GTTTCCTTGC
     4081 GTATTGGGCG CTCTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGGTAAA
     4141 GCCTGGGGTG CCTAATGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG
     4201 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA
     4261 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC
     4321 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC
     4381 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG
     4441 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC
     4501 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA
     4561 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG
     4621 AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG
     4681 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT
     4741 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA
     4801 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
     4861 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
     4921 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTATTAGAAA
     4981 AATTCATCCA GCAGACGATA AAACGCAATA CGCTGGCTAT CCGGTGCCGC AATGCCATAC
     5041 AGCACCAGAA AACGATCCGC CCATTCGCCG CCCAGTTCTT CCGCAATATC ACGGGTGGCC
     5101 AGCGCAATAT CCTGATAACG ATCCGCCACG CCCAGACGGC CGCAATCAAT AAAGCCGCTA
     5161 AAACGGCCAT TTTCCACCAT AATGTTCGGC AGGCACGCAT CACCATGGGT CACCACCAGA
     5221 TCTTCGCCAT CCGGCATGCT CGCTTTCAGA CGCGCAAACA GCTCTGCCGG TGCCAGGCCC
     5281 TGATGTTCTT CATCCAGATC ATCCTGATCC ACCAGGCCCG CTTCCATACG GGTACGCGCA
     5341 CGTTCAATAC GATGTTTCGC CTGATGATCA AACGGACAGG TCGCCGGGTC CAGGGTATGC
     5401 AGACGACGCA TGGCATCCGC CATAATGCTC ACTTTTTCTG CCGGCGCCAG ATGGCTAGAC
     5461 AGCAGATCCT GACCCGGCAC TTCGCCCAGC AGCAGCCAAT CACGGCCCGC TTCGGTCACC
     5521 ACATCCAGCA CCGCCGCACA CGGAACACCG GTGGTGGCCA GCCAGCTCAG ACGCGCCGCT
     5581 TCATCCTGCA GCTCGTTCAG CGCACCGCTC AGATCGGTTT TCACAAACAG CACCGGACGA
     5641 CCCTGCGCGC TCAGACGAAA CACCGCCGCA TCAGAGCAGC CAATGGTCTG CTGCGCCCAA
     5701 TCATAGCCAA ACAGACGTTC CACCCACGCT GCCGGGCTAC CCGCATGCAG GCCATCCTGT
     5761 TCAATCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     5821 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT
     5881 CCCCGAAAAG TGCCACCTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT
     5941 TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT
     6001 CAAAAGAATA GACCGAGATA GGGTTGAGTG GCCGCTACAG GGCGCTCCCA TTCGCCATTC
     6061 AGGCTGCGCA ACTGTTGGGA AGGGCGTTTC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT
     6121 GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC
     6181 ACGACGTTGT AAAACGACGG CCAGTGAGGt aatacgactc actatagggg aattgtgagc
     6241 ggataacaat tcccctctag attt
//