LOCUS       pET_DUET_hRfc2_5_T        9849 bp ds-DNA   circular     10-AUG-2021
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1721..2350
                     /label=hRFC_155-363
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    join(2756..3031,2495..2677)
                     /label=hRFC3
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    2678..2719
                     /label=hRFC3(1)
                     /ApEinfo_label=hRFC3
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    join(1259..1564,1566..1686)
                     /label=hRFC4
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    910..1116
                     /label=hRFC5
                     /ApEinfo_fwdcolor=#cbff64
                     /ApEinfo_revcolor=#cbff64
     misc_feature    join(1688..1690,1692..2350)
                     /label=hRFC4(1)
                     /ApEinfo_label=hRFC4
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     rep_origin      5008..5463
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label=f1 ori
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     misc_feature    join(2756..3031,2495..2677)
                     /label=hRFC3_ORF
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     CDS             complement(5578..6438)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     I
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     S
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     W
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     A
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     S LIKHW"
                     /label=beta-lactamase
                     /ApEinfo_fwdcolor=#19c0cb
                     /ApEinfo_revcolor=pink
     misc_feature    2678..2719
                     /label=hRFC3_ORF(1)
                     /ApEinfo_label=hRFC3_ORF
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     promoter        complement(6439..6531)
                     /gene="bla"
                     /note="AmpR promoter"
                     /label=bla
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     misc_feature    3032..3565
                     /label=hRFC3_2
                     /ApEinfo_fwdcolor=#00ffbb
                     /ApEinfo_revcolor=green
     rep_origin      6612..7200
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label=ori
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     misc_feature    3708..4002
                     /label=hRFC2
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     CDS             complement(7630..7821)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low
                     copy number"
                     /note="rop"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     A DELYRSCLARFGDDGENL"
                     /label=Rop protein, which maintains plasmids at low copy 
                     /ApEinfo_fwdcolor=#19c0cb
                     /ApEinfo_revcolor=pink
     CDS             complement(8393..9475)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /note="lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     L
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSG
                     V
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFS
                     H
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWS
                     A
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSS
                     C
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASP
                     R ALADSLMQLARQVSRLESGQ"
                     /label=lac repressor
                     /ApEinfo_fwdcolor=#19c0cb
                     /ApEinfo_revcolor=pink
     misc_feature    349..1116
                     /label=Rfc5
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    3708..4772
                     /label=hRFC2(1)
                     /ApEinfo_label=hRFC2
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     promoter        complement(9476..9553)
                     /gene="lacI"
                     /note="lacI promoter"
                     /note=" "
                     /label=lacI
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     primer_bind     6..25
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     primer_bind     1171..1190
                     /label=T7(1)
                     /ApEinfo_label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     primer_bind     2407..2426
                     /label=T7(2)
                     /ApEinfo_label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     primer_bind     3620..3639
                     /label=T7(3)
                     /ApEinfo_label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     rep_origin      6536..7218
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     rep_origin      5025..5331
                     /label=F1 ori
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     rep_origin      5008..5463
                     /label=M13 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     misc_binding    25..47
                     /label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
     misc_binding    1190..1212
                     /label=LacO(1)
                     /ApEinfo_label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
     misc_binding    2426..2448
                     /label=LacO(2)
                     /ApEinfo_label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
     misc_binding    3639..3661
                     /label=LacO(3)
                     /ApEinfo_label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
     CDS             complement(8396..9352)
                     /label=LacI
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     CDS             complement(5581..6240)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     misc_feature    94..1116
                     /label=hRFC5(1)
                     /ApEinfo_label=hRFC5
                     /ApEinfo_fwdcolor=#d31eff
                     /ApEinfo_revcolor=green
     misc_feature    2723..2755
                     /label=hRFC3(2)
                     /ApEinfo_label=hRFC3
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    2723..2755
                     /label=hRFC3_ORF(2)
                     /ApEinfo_label=hRFC3_ORF
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
ORIGIN
        1 GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC CTCTAGAAAT
       61 AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGGAAA CCAGCGCCCT GAAACAGCAG
      121 GAACAGCCGG CCGCCACGAA AATTCGCAAC CTGCCGTGGG TGGAAAAATA CCGTCCGCAG
      181 ACCCTGAACG ACCTGATCTC CCACCAGGAT ATCCTGTCTA CCATTCAGAA ATTTATTAAC
      241 GAAGATCGCC TGCCGCATCT GCTGCTGTAT GGTCCGCCGG GCACGGGCAA AACCTCAACC
      301 ATTCTGGCCT GCGCAAAACA GCTGTATAAA GATAAAGAAT TTGGCAGTAT GGTTCTGGAA
      361 CTGAATGCGT CCGACGACCG TGGTATTGAT ATCATTCGCG GCCCGATCCT GTCCTTCGCA
      421 TCTACCCGTA CGATCTTTAA AAAAGGTTTC AAACTGGTCA TTCTGGATGA AGCTGATGCC
      481 ATGACCCAGG ATGCACAGAA TGCGCTGCGC CGTGTGATTG AAAAATTTAC CGAAAACACC
      541 CGTTTTTGCC TGATTTGCAA CTACCTGAGC AAAATCATTC CGGCGCTGCA GAGCCGCTGT
      601 ACCCGTTTTC GTTTTGGTCC GCTGACGCCG GAACTGATGG TTCCGCGTCT GGAACACGTT
      661 GTTGAAGAAG AAAAAGTCGA CATCAGCGAA GATGGTATGA AAGCGCTGGT TACCCTGTCG
      721 AGCGGCGATA TGCGTCGTGC CCTGAATATC CTGCAGTCCA CGAACATGGC GTTTGGCAAA
      781 GTGACCGAAG AAACCGTGTA TACCTGTACG GGCCATCCGC TGAAATCGGA TATCGCAAAT
      841 ATCCTGGATT GGATGCTGAA TCAGGATTTC ACCACCGCAT ATCGTAATAT CACCGAACTG
      901 AAAACCCTGA AAGGCCTGGC GCTGCATGAT ATTCTGACGG AAATTCATCT GTTTGTGCAT
      961 CGCGTGGATT TCCCGTCCAG CGTGCGCATT CATCTGCTGA CCAAAATGGC CGATATTGAA
     1021 TATCGTCTGT CAGTGGGCAC CAATGAAAAA ATTCAGCTGA GTTCTCTGAT CGCCGCCTTT
     1081 CAGGTGACGC GCGACCTGAT CGTTGCAGAA GCATAAGGAT CCACTAGCAT AACCCCTTGG
     1141 GGCCTCTAAA CGGGTCTTGG AGCTCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG
     1201 CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT
     1261 GCAGGCATTT CTGAAAGGTA CCTCCATTTC TACCAAACCG CCGCTGACCA AAGACCGTGG
     1321 TGTCGCAGCA TCCGCGGGCT CGTCTGGCGA AAATAAAAAA GCCAAACCGG TGCCGTGGGT
     1381 TGAAAAATAC CGTCCGAAAT GCGTGGATGA AGTTGCGTTC CAAGAAGAAG TTGTTGCTGT
     1441 GCTGAAAAAA TCGCTGGAAG GTGCCGATCT GCCGAACCTG CTGTTTTATG GCCCGCCGGG
     1501 TACCGGTAAA ACCAGTACCA TTCTGGCCGC TGCTCGTGAA CTGTTTGGCC CGGAACTGTT
     1561 CCGCCTGCGC GTCCTGGAAC TGAATGCCTC AGACGAACGT GGCATCCAGG TTGTGCGCGA
     1621 AAAAGTGAAA AATTTTGCGC AGCTGACCGT TAGCGGCAGT CGTAGCGATG GCAAACCGTG
     1681 TCCGCCGTTT AAAATTGTCA TTCTGGATGA AGCAGATAGT ATGACCTCGG CAGCCCAGGC
     1741 TGCCCTGCGT CGTACGATGG AAAAAGAATC TAAAACCACG CGCTTTTGTC TGATTTGCAA
     1801 TTATGTCTCT CGCATCATTG AACCGCTGAC CAGCCGTTGC TCTAAATTCC GTTTTAAACC
     1861 GCTGTCTGAT AAAATTCAGC AGCAGCGCCT GCTGGATATC GCGAAAAAAG AAAACGTCAA
     1921 AATCAGTGAC GAAGGCATCG CGTATCTGGT GAAAGTCTCA GAAGGCGATC TGCGCAAAGC
     1981 GATTACCTTC CTGCAGAGCG CAACCCGCCT GACGGGTGGC AAAGAAATTA CCGAAAAAGT
     2041 GATTACCGAT ATTGCAGGCG TCATCCCGGC AGAAAAAATT GATGGTGTGT TTGCGGCGTG
     2101 CCAGAGCGGT AGCTTTGACA AACTGGAAGC CGTGGTGAAA GACCTGATTG ATGAAGGTCA
     2161 TGCTGCGACC CAGCTGGTTA ATCAACTGCA CGACGTTGTT GTGGAAAACA ACCTGTCGGA
     2221 CAAACAGAAA TCAATTATTA CCGAAAAACT GGCTGAAGTG GATAAATGCC TGGCAGATGG
     2281 TGCAGACGAA CACCTGCAGC TGATTAGTCT GTGCGCGACC GTTATGCAGC AGCTGTCGCA
     2341 GAACTGCTAA GGATCCACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGGCGGCCG
     2401 CGAAATTAAT ACGACTCACT ATAGGGGAAT TGTGAGCGGA TAACAATTCC CCTCTAGAAA
     2461 TAATTTTGTT TAACTTTAAG AAGGAGATAT ACATATGAGC CTGTGGGTTG ATAAATATCG
     2521 TCCGTGCAGC CTGGGCCGCC TGGATTATCA TAAAGAACAG GCGGCGCAGC TGCGCAATCT
     2581 GGTGCAGTGC GGCGATTTTC CGCATCTGCT GGTGTATGGT CCGAGCGGCG CGGGCAAGAA
     2641 AACCCGTATT ATGTGTATTC TGCGTGAACT GTATGGCGTG GGCGTTGAAA AACTGCGCAT
     2701 TGAACATCAG ACCATTACCG ATCCGAGCAA AAAGAAAATT GAAATTAGCA CCATTGCGAG
     2761 CAACTATCAT CTGGAAGTGA ACCCGAGCGA TGCGGGCAAT AGCGATCGCG TGGTGATTCA
     2821 GGAAATGCTG AAAACCGTGG CGCAGAGCCA GCAGCTGGAA ACCAACAGCC AGCGCGATTT
     2881 TAAAGTGGTG CTGCTGACCG AAGTGGATAA ACTGACCAAA GATGCGCAGC ATGCGCTGCG
     2941 CCGTACCATG GAAAAATATA TGAGCACCTG CCGCCTGATT CTGTGTTGCA ACAGCACCAG
     3001 CAAAGTGATT CCGCCGATTC GTAGCCGTTG CCTGGCGGTG CGCGTGCCGG CGCCGAGCAT
     3061 TGAAGATATT TGTCATGTGC TGAGCACCGT GTGCAAAAAA GAAGGCCTGA ACCTGCCGAG
     3121 CCAGCTGGCG CATCGTCTGG CGGAAAAAAG CTGCCGTAAC CTGCGCAAAG CGCTGCTGAT
     3181 GTGTGAAGCG TGCCGCGTGC AGCAGTATCC GTTTACCGCG GATCAGGAAA TTCCGGAAAC
     3241 CGATTGGGAA GTGTATCTGC GCGAAACCGC GAATGCGATT GTTAGCCAGC AGACCCCGCA
     3301 GCGTCTGCTG GAAGTGCGTG GCCGTCTGTA TGAACTGCTG ACCCATTGCA TTCCGCCGGA
     3361 AATTATTATG AAAGGCCTGC TGAGCGAACT GCTGCATAAT TGCGATGGCC AGCTGAAAGG
     3421 CGAAGTGGCG CAGATGGCGG CGTATTATGA ACATCGCCTG CAGCTGGGTA GCAAAGCGAT
     3481 TTATCATCTG GAAGCGTTTG TTGCGAAATT TATGGCGCTG TATAAAAAAT TTATGGAAGA
     3541 TGGCCTGGAA GGCATGATGT TTTGAGGATC CACTAGCATA ACCCCTTGGG GCCTCTAAAC
     3601 GGGTCTTGAG ATCTGAAATT AATACGACTC ACTATAGGGG AATTGTGAGC GGATAACAAT
     3661 TCCCCTCTAG AAATAATTTT GTTTAACTTT AAGAAGGAGA TATACATATG GAAGTGGAAG
     3721 CGGTTTGCGG TGGTGCCGGC GAAGTTGAAG CGCAGGATAG CGATCCGGCC CCGGCGTTTA
     3781 GCAAAGCCCC GGGTAGCGCG GGCCATTATG AACTGCCGTG GGTTGAAAAA TATCGTCCGG
     3841 TTAAACTGAA CGAAATTGTT GGTAACGAAG ATACCGTTAG CCGCCTGGAA GTGTTTGCGC
     3901 GTGAAGGCAA CGTGCCGAAT ATTATTATCG CGGGCCCGCC GGGCACCGGC AAAACCACCA
     3961 GCATTCTGTG TCTGGCGCGC GCCCTGCTGG GTCCGGCGCT GAAAGATGCC ATGCTGGAAC
     4021 TGAACGCGAG CAACGATCGC GGCATTGATG TGGTTCGCAA CAAAATCAAA ATGTTCGCGC
     4081 AGCAGAAAGT TACCCTGCCG AAAGGTCGTC ACAAAATTAT TATTCTGGAT GAAGCGGATA
     4141 GCATGACCGA TGGCGCGCAG CAGGCGCTGC GTCGCACCAT GGAAATCTAT AGCAAAACCA
     4201 CCCGTTTTGC GCTGGCGTGC AATGCCAGCG ATAAAATTAT CGAACCGATC CAGAGCCGCT
     4261 GCGCGGTGCT GCGCTATACC AAACTGACCG ATGCCCAGAT CCTGACCCGT CTGATGAATG
     4321 TGATTGAAAA AGAACGCGTG CCGTATACCG ATGATGGCCT GGAAGCGATC ATTTTTACCG
     4381 CGCAGGGTGA TATGCGTCAG GCCCTGAACA ACCTGCAGAG CACCTTTAGC GGCTTTGGTT
     4441 TTATTAACAG CGAAAACGTG TTTAAAGTGT GCGATGAACC GCATCCGCTG CTGGTGAAAG
     4501 AAATGATTCA GCATTGCGTG AATGCCAATA TTGATGAAGC GTATAAAATT CTGGCGCATC
     4561 TGTGGCACCT GGGTTATAGC CCGGAAGATA TTATCGGCAA TATCTTTCGC GTTTGCAAAA
     4621 CCTTTCAGAT GGCGGAATAT CTGAAACTGG AATTTATTAA AGAAATTGGC TATACCCACA
     4681 TGAAAATTGC GGAAGGTGTT AATAGCCTGC TGCAGATGGC GGGCCTGCTG GCCCGCCTGT
     4741 GCCAGAAAAC CATGGCCCCG GTGGCCAGCT AAGGATCCAC TAGCATAACC CCTTGGGGCC
     4801 TCTAAACGGG TCTTGCTCGA GTCTGGTAAA GAAACCGCTG CTGCGAAATT TGAACGCCAG
     4861 CACATGGACT CGTCTACTAG CGCAGCTTAA TTAACCTAGG CTGCTGCCAC CGCTGAGCAA
     4921 TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA
     4981 GGAACTATAT CCGGATTGGC GAATGGGacg cgccctgtag cggcgcatta agcgcggcgg
     5041 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5101 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5161 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5221 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5281 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5341 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5401 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     5461 tttctggcgg cacgatggca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     5521 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     5581 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     5641 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     5701 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     5761 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     5821 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     5881 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     5941 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     6001 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     6061 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     6121 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     6181 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     6241 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     6301 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     6361 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     6421 gaaatgttga atactcatac tcttcctttt tcaatcatga ttgaagcatt tatcagggtt
     6481 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggtcatg
     6541 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6601 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6661 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6721 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     6781 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6841 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6901 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6961 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7021 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7081 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7141 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7201 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7261 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7321 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7381 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat
     7441 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     7501 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     7561 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     7621 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag
     7681 ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc
     7741 gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc
     7801 ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt
     7861 aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc
     7921 ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag
     7981 aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg
     8041 tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg
     8101 cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc
     8161 agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta
     8221 accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgct
     8281 agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg
     8341 tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc
     8401 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8461 gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca
     8521 acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg
     8581 tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc
     8641 tgtcttcggt atcgtcgtat cccactaccg agatgtccgc accaacgcgc agcccggact
     8701 cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg
     8761 gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt
     8821 cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag
     8881 ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt
     8941 gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa
     9001 tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg
     9061 cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga
     9121 cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta
     9181 ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa
     9241 tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt
     9301 tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt
     9361 ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg
     9421 tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca
     9481 ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc
     9541 attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag
     9601 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag
     9661 atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg
     9721 ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg
     9781 ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc
     9841 gagatcgat     
//
